LOCUS       CN2364-rAAV-eHGT_523h-minBglobin-SYFP2-WPRE3-BGHpA        4857 bp    DNA     circular UNA 19-NOV-2019
DEFINITION  Gibson/Homology Cloning ligation product.
ACCESSION   urn.local...38h-b7vgqd4
VERSION     urn.local...38h-b7vgqd4
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /vntifkey=21
                     /locus_tag="L-ITR"
                     /ApEinfo_label="L-ITR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="L-ITR"
     misc_feature    complement(137..799)
                     /Source="referenceToDocument: urn:local:.:113-b7vf5z2"
                     /label="h523-RMSYFP2_seqR.ab1 (concatenated sequence 2)
                     (reversed)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    136^137
                     /Original_Bases="AGCGCGCNNGANGAGNGAGTGGCCAACTCCATCACTAGGGG
                     "
                     /label="AGCGCGCNNGANGAGNGAGTGGCCAACTCCATCACTAGGGG"
                     /note="Geneious type: Editing History Deletion"
     ligation        137..154
                     /label="Ligation"
     primer_bind     137..155
                     /Mismatches=0
                     /%GC="73.7"
                     /Hairpin_Tm="60.9"
                     /Self_Dimer_Tm="46.9"
                     /Tm="70.6"
                     /annotation_group="37090129783222967f6c89ce-f5b7-4ca9-99c6
                     -d41b791b8931"
                     /Sequence="TTCCTGCGGCCGCACGCGT"
                     /created_by="primer3"
                     /label="Gibson Assembly into CN1244 F'"
     misc_feature    156..775
                     /created_by="refugio.martinez"
                     /label="eHGT_523h"
     primer_bind     complement(776..799)
                     /Mismatches=0
                     /%GC="58.3"
                     /Hairpin_Tm="51.4"
                     /Self_Dimer_Tm="2.7"
                     /Tm="65.8"
                     /annotation_group="370901332325276730502e9a-c09a-494c-a385
                     -485471b2c645"
                     /Sequence="GACTTTTATGCCCAGCCCGAGCTC"
                     /created_by="primer3"
                     /label="Gibson Assembly into CN1244 R'"
     misc_feature    781..154
                     /Source="referenceToDocument: urn:local:.:79-akukj0o"
                     /label="CN1244_pAAV Linearized (digested by SacI, MluI)
                     Fragment 3 (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     ligation        781..799
                     /label="Ligation"
     misc_feature    782..834
                     /vntifkey=21
                     /locus_tag="Beta\Globin\minimal\promoter"
                     /ApEinfo_label="Beta\Globin\minimal\promoter"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Beta\Globin\minimal\promoter"
     misc_feature    791..796
                     /vntifkey=21
                     /locus_tag="TATA\box"
                     /ApEinfo_label="TATA\box"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="TATA\box"
     misc_feature    799^800
                     /Original_Bases="AGGGCAGAGCCATCTATTGCTTACANNGCTTCNGGGANCCA
                     GNNNNNNNNNNNNNNNNNNNNN"
                     /label="AGGGCAGAGCCATCTATTGCTTACANNGCTTCNGGGANCCAGNNNNNNNN
                     NNNNNNNNNNNNN"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    823
                     /vntifkey=21
                     /locus_tag="transcriptional\start"
                     /ApEinfo_label="transcriptional\start"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="transcriptional\start"
     misc_feature    875..1594
                     /vntifkey=21
                     /locus_tag="SYFP2"
                     /ApEinfo_label="SYFP2"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="SYFP2"
     misc_feature    1629..1874
                     /vntifkey=21
                     /locus_tag="WPRE3"
                     /ApEinfo_label="WPRE3"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="WPRE3"
     misc_feature    1888..2091
                     /vntifkey=21
                     /locus_tag="BGH-pA"
                     /ApEinfo_label="BGH-pA"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="BGH-pA"
     primer          complement(2002..2021)
                     /vntifkey=27
                     /locus_tag="BGH-R"
                     /ApEinfo_label="BGH-R"
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#80ff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="BGH-R"
     misc_feature    2120..2260
                     /vntifkey=21
                     /locus_tag="R-ITR"
                     /ApEinfo_label="R-ITR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="R-ITR"
     rep_origin      2352..2658
                     /vntifkey=33
                     /locus_tag="f1\Origin"
                     /ApEinfo_label="f1\Origin"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="f1\Origin"
     misc_feature    3177..4034
                     /vntifkey=21
                     /locus_tag="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="AmpR"
     rep_origin      4185..4852
                     /vntifkey=33
                     /locus_tag="pUC\ori"
                     /ApEinfo_label="pUC\ori"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="pUC\ori"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtcaggg gtgtggcatc agctatctgg
      181 ataaaaatga ggaattaaga gaatagcaga taacactagt acacagagta gtaaagtaag
      241 attggtgatg attttcaatc cacctctact tgggcttttt attcccttcg tcttaatttt
      301 ttctttctgt ctgatgttct ttggaaaaaa tcagagtgtg gcaagttggt cttgctcgta
      361 ggggtgctag aagaagttac atttacccaa tgcattggat attagatata cccaagataa
      421 ctgtcaggtc aggtggtgtg aagacaggga agattttgga gccagtaatc gggaatataa
      481 tgggggaagg aagagaagcc agaaaacaaa gactaaagta gtggttctta actctggctg
      541 tatattacag cacatatggt cctttggaaa aacacggaag ctggtgcccc acattcagag
      601 attcagatat aatcagagat tcagatataa taggtctgga tgaagcttgg gcattgctat
      661 ttctccaaat ctttctagat tattccaaag cacaggcaag actaaaaatt aatgtaaact
      721 aagagtaagg gtccctagta gatctttttc atatatgatg cgaagcccag gttcagagct
      781 cgggctgggc ataaaagtca gggcagagcc atctattgct tacatttgct tctgggatcc
      841 agatctttcg aagctagcgc taccggtcgc caccatggtg agcaagggcg aggagctgtt
      901 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      961 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agctgatctg
     1021 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctgg gctacggcgt
     1081 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     1141 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     1201 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     1261 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     1321 caacgtctat atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg
     1381 ccacaacatc gaggacggcg gcgtgcagct cgccgaccac taccagcaga acacccccat
     1441 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccaagctgag
     1501 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     1561 gatcactctc ggcatggacg agctgtacaa gtaagtcgac ggcgcgccgc ggccgcgaat
     1621 tcgatatcat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     1681 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     1741 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttag ttcttgccac
     1801 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     1861 tgacaattcc gtggctcgag agatcttcga ctgtgccttc tagttgccag ccatctgttg
     1921 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     1981 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     2041 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gagatctcac
     2101 gtgcggaccg agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc
     2161 gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg
     2221 gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt
     2281 ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc
     2341 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     2401 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     2461 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     2521 tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc
     2581 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     2641 tgttccaaac tggaacaaca ctcaacccta tctcgggcta ttcttttgat ttataaggga
     2701 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     2761 attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg
     2821 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     2881 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     2941 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     3001 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     3061 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     3121 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     3181 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     3241 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     3301 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     3361 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     3421 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     3481 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     3541 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     3601 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     3661 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     3721 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     3781 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     3841 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     3901 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     3961 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     4021 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     4081 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     4141 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     4201 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     4261 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     4321 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     4381 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     4441 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     4501 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     4561 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     4621 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     4681 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     4741 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     4801 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgt
//
