LOCUS       CN2425-rAAV-eHGT_465m-minBglobin-SYFP2-WPRE3-BGHpA        4629 bp    DNA     circular UNA 11-DEC-2019
DEFINITION  Gibson/Homology Cloning ligation product.
ACCESSION   urn.local...8w8-baztwu5
VERSION     urn.local...8w8-baztwu5
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /vntifkey=21
                     /locus_tag="L-ITR"
                     /ApEinfo_label="L-ITR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="L-ITR"
     misc_feature    complement(137..571)
                     /Source="referenceToDocument: urn:local:.:8av-baztis9"
                     /label="465m-RMSYFP2seqR.ab1 (concatenated sequence 2)
                     (reversed)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    136^137
                     /Original_Bases="GAGCGCGCAGAGAGGGAGTGGCCAACTCCATCACTAGGGG"
                     /label="GAGCGCGCAGAGAGGGAGTGGCCAACTCCATCACTAGGGG"
                     /note="Geneious type: Editing History Deletion"
     ligation        137..154
                     /label="Ligation"
     primer_bind     137..155
                     /Mismatches=0
                     /%GC="73.7"
                     /Hairpin_Tm="60.9"
                     /Self_Dimer_Tm="46.9"
                     /Tm="70.6"
                     /Pair_Dimer_Tm="None"
                     /annotation_group="559076725184158066c97c9b-2832-4624-8b12
                     -2c5a76791314"
                     /Sequence="TTCCTGCGGCCGCACGCGT"
                     /created_by="primer3"
                     /Product_Size=435
                     /label="Gibson Assembly into CN1244 F'"
     misc_feature    156..547
                     /created_by="refugio.martinez"
                     /label="eHGT_465m"
     primer_bind     complement(548..571)
                     /Mismatches=0
                     /%GC="58.3"
                     /Hairpin_Tm="51.4"
                     /Self_Dimer_Tm="2.7"
                     /Tm="65.8"
                     /Pair_Dimer_Tm="None"
                     /annotation_group="559076725184158066c97c9b-2832-4624-8b12
                     -2c5a76791314"
                     /Sequence="GACTTTTATGCCCAGCCCGAGCTC"
                     /created_by="primer3"
                     /Product_Size=435
                     /label="Gibson Assembly into CN1244 R'"
     misc_feature    553..154
                     /Source="referenceToDocument: urn:local:.:3by-b7vj7bw"
                     /label="CN1244_pAAV Linearized (digested by SacI, MluI)
                     Fragment 3 (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     ligation        553..571
                     /label="Ligation"
     misc_feature    554..606
                     /vntifkey=21
                     /locus_tag="Beta\Globin\minimal\promoter"
                     /ApEinfo_label="Beta\Globin\minimal\promoter"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Beta\Globin\minimal\promoter"
     misc_feature    563..568
                     /vntifkey=21
                     /locus_tag="TATA\box"
                     /ApEinfo_label="TATA\box"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="TATA\box"
     misc_feature    571^572
                     /Original_Bases="AGGGCAGAGCCATCTATTGCTTNCATTNNNNNNNNNNNNNN
                     NNNNNTNNNNNNNNNNNNNNNNNNNNNNNN"
                     /label="AGGGCAGAGCCATCTATTGCTTNCATTNNNNNNNNNNNNNNNNNNNTNNN
                     NNNNNNNNNNNNNNNNNNNNN"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    595
                     /vntifkey=21
                     /locus_tag="transcriptional\start"
                     /ApEinfo_label="transcriptional\start"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="transcriptional\start"
     misc_feature    647..1366
                     /vntifkey=21
                     /locus_tag="SYFP2"
                     /ApEinfo_label="SYFP2"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="SYFP2"
     misc_feature    1401..1646
                     /vntifkey=21
                     /locus_tag="WPRE3"
                     /ApEinfo_label="WPRE3"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="WPRE3"
     misc_feature    1660..1863
                     /vntifkey=21
                     /locus_tag="BGH-pA"
                     /ApEinfo_label="BGH-pA"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="BGH-pA"
     primer          complement(1774..1793)
                     /vntifkey=27
                     /locus_tag="BGH-R"
                     /ApEinfo_label="BGH-R"
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#80ff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="BGH-R"
     misc_feature    1892..2032
                     /vntifkey=21
                     /locus_tag="R-ITR"
                     /ApEinfo_label="R-ITR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="R-ITR"
     rep_origin      2124..2430
                     /vntifkey=33
                     /locus_tag="f1\Origin"
                     /ApEinfo_label="f1\Origin"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="f1\Origin"
     misc_feature    2949..3806
                     /vntifkey=21
                     /locus_tag="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="AmpR"
     rep_origin      3957..4624
                     /vntifkey=33
                     /locus_tag="pUC\ori"
                     /ApEinfo_label="pUC\ori"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="pUC\ori"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtactga ccgtttgcac agtgcctatg
      181 ttcatttata ataaacctgc gtgtcacgaa ttccaaccca aagtgtgtgc gaataatgga
      241 aaatataaga aaaatctact taagcatctg gcaggaaaat accaataatt atggtgtttc
      301 aagtcaattt gtttcttttt caagattttc tttgaaagta tacatgaaac ctgtaaaaag
      361 gaaactgatt gcttcctcag ggtaaacagc tctcgatgat agaagtcatt tacttttgat
      421 ttccttttag gtgagtattc agaacgatac tcaaatgtga aacaaattta atcaagggga
      481 aaaaggtgca aaaatactat ggaaaatgtc aatcactagt tttatggaga agctgaaaat
      541 tcgtcctgag ctcgggctgg gcataaaagt cagggcagag ccatctattg cttacatttg
      601 cttctgggat ccagatcttt cgaagctagc gctaccggtc gccaccatgg tgagcaaggg
      661 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
      721 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
      781 gaagctgatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
      841 gggctacggc gtgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt
      901 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
      961 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     1021 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     1081 ctacaacagc cacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa
     1141 cttcaagatc cgccacaaca tcgaggacgg cggcgtgcag ctcgccgacc actaccagca
     1201 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctacca
     1261 gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     1321 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaagtcg acggcgcgcc
     1381 gcggccgcga attcgatatc ataatcaacc tctggattac aaaatttgtg aaagattgac
     1441 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     1501 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     1561 agttcttgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     1621 gctgttgggc actgacaatt ccgtggctcg agagatcttc gactgtgcct tctagttgcc
     1681 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     1741 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     1801 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     1861 atgagatctc acgtgcggac cgagcggccg caggaacccc tagtgatgga gttggccact
     1921 ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg
     1981 ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg
     2041 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac
     2101 catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     2161 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     2221 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     2281 gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta
     2341 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     2401 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcgggc tattcttttg
     2461 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     2521 aatttaacgc gaattttaac aaaatattaa cgtttacaat tttatggtgc actctcagta
     2581 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
     2641 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     2701 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
     2761 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     2821 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     2881 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     2941 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     3001 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     3061 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     3121 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     3181 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     3241 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     3301 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     3361 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     3421 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     3481 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     3541 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     3601 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     3661 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     3721 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     3781 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     3841 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     3901 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     3961 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     4021 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     4081 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     4141 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     4201 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     4261 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     4321 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     4381 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     4441 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     4501 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     4561 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     4621 ctcacatgt
//
