LOCUS       CN2471\pAAV-eHGT_536h-minBG-SYFP2-WPRE3-BGHpA        4559 bp    DNA     circular     13-DEC-2019
SOURCE      
  ORGANISM  
COMMENT     rAAV-ESYN-SYFP2-WPRE3-BGHpA
            clone #8-1 correct , 8-2 backup
            
            
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-13120942|
COMMENT     VNTDBDATE|-13120942|
COMMENT     LSOWNER|
COMMENT     VNTNAME|CN2471 pAAV-eHGT_536h-minBG-SYFP2-WPRE3-BGHpA|
COMMENT     VNTAUTHORNAME|Jonathan Ting|
COMMENT     VNTOAUTHORNAME|Feng Zhang|
FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /vntifkey="21"
                     /label=L-ITR
     misc_feature    1822..1962
                     /vntifkey="21"
                     /label=R-ITR
     rep_origin      2054..2360
                     /vntifkey="33"
                     /label=f1\Origin
     misc_feature    2879..3736
                     /vntifkey="21"
                     /label=AmpR
     rep_origin      3887..4554
                     /vntifkey="33"
                     /label=pUC\ori
     primer          complement(1710..1729)
                     /vntifkey="27"
                     /label=BGH-R
     misc_feature    1596..1799
                     /vntifkey="21"
                     /label=BGH-pA
     misc_feature    1337..1582
                     /vntifkey="21"
                     /label=WPRE3
     misc_feature    583..1302
                     /vntifkey="21"
                     /label=SYFP2
     misc_feature    490..542
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=Beta\Globin\minimal\promoter
     misc_feature    499..504
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=TATA\box
     misc_feature    531..531
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=transcriptional\start
     primer_bind     156..173
                     /%GC="66.7"
                     /Hairpin_Tm="40.2"
                     /Self_Dimer_Tm="None"
                     /Tm="62.1"
                     /Sequence="CCAGGAGCAAAGCAGCGC"
                     /created_by="primer3"
                     /annotation_group="1190081581932442fd16773b-0e7c-489e-8eda
                     -b9812b67edf9eHGT_536h.fasta"
                     /#_Off-target_Sites=0
                     /Off-target_Template="eHGT_536h.fasta"
                     /vntifkey="28"
                     /label=33\F
     primer_bind     complement(462..483)
                     /Sequence="TCTTCAAAGGAGAAGCCACAGG"
                     /Hairpin_Tm="43.6"
                     /Tm="60.2"
                     /Self_Dimer_Tm="None"
                     /%GC="50.0"
                     /created_by="primer3"
                     /vntifkey="28"
                     /label=536h_R
     misc_feature    156..483
                     /vntifkey="21"
                     /label=eHGT_536h
BASE COUNT     1033 a      1246 c      1206 g      1074 t 
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 
      121 actccatcac taggggttcc tgcggccgca cgcgtccagg agcaaagcag cgcgccaggc 
      181 tggagctgtt ccgtcattac cgccacgtct gcgcttcggt tgtgagaacc gattcgttac 
      241 cctaacctct taggactaag agccagtctt ctggtggcgc tcgctttcac ttacttttta 
      301 tttccctttc tattcccgcg agctaggggg agaaagtgta aatcccgccc gcttctccag 
      361 ggctgaaaat ggagaggctg aggcggcttg gagagtgcgc actgcgcatg ctccgcagag 
      421 catccccgca ggtcaaccgg ggcaagggag agacatgaaa gcctgtggct tctcctttga 
      481 agagagctcg ggctgggcat aaaagtcagg gcagagccat ctattgctta catttgcttc 
      541 tgggatccag atctttcgaa gctagcgcta ccggtcgcca ccatggtgag caagggcgag 
      601 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac 
      661 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag 
      721 ctgatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgggc 
      781 tacggcgtgc agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag 
      841 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac 
      901 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg 
      961 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac 
     1021 aacagccaca acgtctatat caccgccgac aagcagaaga acggcatcaa ggccaacttc 
     1081 aagatccgcc acaacatcga ggacggcggc gtgcagctcg ccgaccacta ccagcagaac 
     1141 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag ctaccagtcc 
     1201 aagctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc 
     1261 gccgccggga tcactctcgg catggacgag ctgtacaagt aagtcgacgg cgcgccgcgg 
     1321 ccgcgaattc gatatcataa tcaacctctg gattacaaaa tttgtgaaag attgactggt 
     1381 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat 
     1441 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttagtt 
     1501 cttgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg 
     1561 ttgggcactg acaattccgt ggctcgagag atcttcgact gtgccttcta gttgccagcc 
     1621 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt 
     1681 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct 
     1741 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc 
     1801 acgtgcggac cgagcggccg caggaacccc tagtgatgga gttggccact ccctctctgc 
     1861 gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc 
     1921 gggcggcctc agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt 
     1981 ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc 
     2041 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac 
     2101 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt 
     2161 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc 
     2221 tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc 
     2281 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact 
     2341 cttgttccaa actggaacaa cactcaaccc tatctcgggc tattcttttg atttataagg 
     2401 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc 
     2461 gaattttaac aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc 
     2521 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg 
     2581 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat 
     2641 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg 
     2701 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt 
     2761 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 
     2821 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat 
     2881 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt 
     2941 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg 
     3001 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga 
     3061 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg 
     3121 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt 
     3181 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg 
     3241 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg 
     3301 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga 
     3361 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc 
     3421 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc 
     3481 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc 
     3541 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg 
     3601 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac 
     3661 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc 
     3721 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt 
     3781 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac 
     3841 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa 
     3901 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc 
     3961 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt 
     4021 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg 
     4081 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc 
     4141 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt 
     4201 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga 
     4261 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct 
     4321 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg 
     4381 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca 
     4441 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa 
     4501 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgt 
//
