LOCUS       CN2570_pAAV-3xcore_eHGT_128h-minBglobin-SYFP2-WPRE3-BGHpA        5515 bp    DNA     circular UNA 11-APR-2020
DEFINITION  .
ACCESSION   urn.local...2a-bsfxu9o
VERSION     urn.local...2a-bsfxu9o
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     ##Comment-START##
            Comment :: rAAV-ESYN-SYFP2-WPRE3-BGHpA
                       clone #8-1 correct , 8-2 backup
            ##Comment-END##
            ##Imported_From-START##
            Path     :: C:\VNTI Database
            Filename :: _exdata.ma4
            ##Imported_From-END##
FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /label="L-ITR"
     misc_feature    156..1438
                     /Original_Bases="ATAGGTACCGAGCTCTATGCACTCACAGTGGTTTGGCATGC
                     ATCTGGTGAATTTTTTTTAACGAAAAATTAGTGTTGGTTTCGATGTATGGTAGCATTC
                     TCCCTAACGTAATTTGAATAATTCAGCAAAGCCCCACTACCAGCTGTACTTCTGCAGC
                     CTCTTCCATTCTTTTCAGCATTATAATTTTGGTTAATTTTCAATTTTAGGTCCTACGT
                     CTCTGCAATTTGTGTATGAATAACAGAATAATTTCCCTCTTTTGTTTCGCCTTTCCTG
                     TTCCTGAATCTAAATAAAGATGGCTTTTTAGTATTAAAAGTGGAAGAAAATTACAGGT
                     AATTATCTTTGACGGTAAAAACGCTGTAATCAGCGGGCTACATGAAAAATTACTCTAA
                     TTATGGCTGCATTTAAGAGAATGGAAAAAAACCTTCTTGTGGATAAAAACCTTAAATT
                     GTCCCCAATGTCTGCTTCAAATTGGATGGCACTGCAGCTGGAGGCTTTGTTCAGAATT
                     GATCCTGGGGAGCTACGAACCCAAAGTTTCACAGTAG"
                     /label="ATAGGTACCGAGCTCTATGCACTCACAGTGGTTTGGCATGCATCTGGTGA
                     ATTTTTTTTAACGAAAAATTAGTGTTGGTTTCGATGTATGGTAGCATTCTCCCTAACG
                     TAATTTGAATAATTCAGCAAAGCCCCACTACCAGCTGTACTTCTGCAGCCTCTTCCAT
                     TCTTTTCAGCATTATAATTTTGGTTAATTTTCAATTTTAGGTCCTACGTCTCTGCAAT
                     TTGTGTATGAATAACAGAATAATTTCCCTCTTTTGTTTCGCCTTTCCTGTTCCTGAAT
                     CTAAATAAAGATGGCTTTTTAGTATTAAAAGTGGAAGAAAATTACAGGTAATTATCTT
                     TGACGGTAAAAACGCTGTAATCAGCGGGCTACATGAAAAATTACTCTAATTATGGCTG
                     CATTTAAGAGAATGGAAAAAAACCTTCTTGTGGATAAAAACCTTAAATTGTCCCCAAT
                     GTCTGCTTCAAATTGGATGGCACTGCAGCTGGAGGCTTTGTTCAGAATTGATCCTGGG
                     GAGCTACGAACCCAAAGTTTCACAGTAG"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    156..1439
                     /created_by="ximena.opitzaraya"
                     /label="3xcore_eHGT_128h"
     misc_feature    1446..1498
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Beta Globin minimal promoter"
     misc_feature    1455..1460
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="TATA box"
     misc_feature    1487
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="transcriptional start"
     misc_feature    1539..2258
                     /label="SYFP2"
     primer_bind     1585..1605
                     /label="EGFP-N-R"
     misc_feature    2293..2538
                     /label="WPRE3"
     misc_feature    2552..2755
                     /label="BGH-pA"
     primer          complement(2666..2685)
                     /label="BGH-R"
     misc_feature    2778..2918
                     /label="R-ITR"
     rep_origin      3010..3316
                     /label="f1 Origin"
     misc_feature    3835..4692
                     /label="AmpR"
     rep_origin      4843..5510
                     /label="pUC ori"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtctagt atcatgagga ggatctcaaa
      181 gcagggaagg gaagccagct gagggacccc tgagaggaga tcaggggata aatactttgc
      241 aacagaatcc ccatgggaaa catgacaaaa ttagtaacag gaatggagcc tccctcagag
      301 gatacatgaa tcgttgagtt gaaaataacc acaaggtcat caaatccagc tgccaccaac
      361 tgcctgaata ttttcaacaa atttttgaac aagcagtcct ccagcctcct ccagctgcat
      421 ccagcagctc agtgctccac ctgacagcct cttctgatag cgtgaaggct cagaaagccc
      481 ctgtggggcc cagcacatcc tggggcaggc ctctagtggg cagagggcag catactgggg
      541 tctggatcta acacactcct ccatccaggt ggtatattta tagctagtat catgaggagg
      601 atctcaaagc agggaaggga agccagctga gggacccctg agaggagatc aggggataaa
      661 tactttgcaa cagaatcccc atgggaaaca tgacaaaatt agtaacagga atggagcctc
      721 cctcagagga tacatgaatc gttgagttga aaataaccac aaggtcatca aatccagctg
      781 ccaccaactg cctgaatatt ttcaacaaat ttttgaacaa gcagtcctcc agcctcctcc
      841 agctgcatcc agcagctcag tgctccacct gacagcctct tctgatagcg tgaaggctca
      901 gaaagcccct gtggggccca gcacatcctg gggcaggcct ctagtgggca gagggcagca
      961 tactggggtc tggatctaac acactcctcc atccaggtgg tatatttata gctagtatca
     1021 tgaggaggat ctcaaagcag ggaagggaag ccagctgagg gacccctgag aggagatcag
     1081 gggataaata ctttgcaaca gaatccccat gggaaacatg acaaaattag taacaggaat
     1141 ggagcctccc tcagaggata catgaatcgt tgagttgaaa ataaccacaa ggtcatcaaa
     1201 tccagctgcc accaactgcc tgaatatttt caacaaattt ttgaacaagc agtcctccag
     1261 cctcctccag ctgcatccag cagctcagtg ctccacctga cagcctcttc tgatagcgtg
     1321 aaggctcaga aagcccctgt ggggcccagc acatcctggg gcaggcctct agtgggcaga
     1381 gggcagcata ctggggtctg gatctaacac actcctccat ccaggtggta tatttatagg
     1441 agctcgggct gggcataaaa gtcagggcag agccatctat tgcttacatt tgcttctggg
     1501 atccagatct ttcgaagcta gcgctaccgg tcgccaccat ggtgagcaag ggcgaggagc
     1561 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     1621 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagctga
     1681 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgggctacg
     1741 gcgtgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     1801 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     1861 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     1921 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     1981 gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaaga
     2041 tccgccacaa catcgaggac ggcggcgtgc agctcgccga ccactaccag cagaacaccc
     2101 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagctac cagtccaagc
     2161 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     2221 ccgggatcac tctcggcatg gacgagctgt acaagtaagt cgacggcgcg ccgcggccgc
     2281 gaattcgata tcataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     2341 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     2401 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttagttcttg
     2461 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     2521 gcactgacaa ttccgtggct cgagagatct tcgactgtgc cttctagttg ccagccatct
     2581 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     2641 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     2701 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgcacgt
     2761 gcggaccgag cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc
     2821 tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc
     2881 ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct
     2941 ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc
     3001 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     3061 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     3121 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     3181 cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc
     3241 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     3301 ttccaaactg gaacaacact caaccctatc tcgggctatt cttttgattt ataagggatt
     3361 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     3421 tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat
     3481 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     3541 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     3601 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
     3661 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     3721 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     3781 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     3841 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     3901 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     3961 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     4021 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     4081 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     4141 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     4201 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     4261 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     4321 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     4381 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     4441 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     4501 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     4561 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     4621 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     4681 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     4741 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     4801 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     4861 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     4921 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     4981 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     5041 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     5101 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     5161 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     5221 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     5281 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     5341 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     5401 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     5461 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgt
//
