LOCUS       CN2582\pAAV-eHGT_486m(3xCore)-minBG-SYFP2-WPRE3-BGHpA\(JT)        4969 bp    DNA     circular      7-JUL-2020
SOURCE      
  ORGANISM  
COMMENT     rAAV-hPTenh(FAM84b)-minBG-SYFP2-WPRE3-BGHpA
            
            clones 2,3,4 all good
            clone 1 low yield 
            
            
            
            
            
            
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-13418709|
COMMENT     VNTDBDATE|-13418709|
COMMENT     LSOWNER|
COMMENT     VNTNAME|CN2582 pAAV-eHGT_486m(3xCore)-minBG-SYFP2-WPRE3-BGHpA (JT)|
COMMENT     VNTAUTHORNAME|Jonathan Ting|
COMMENT     VNTOAUTHORNAME|Feng Zhang|
FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /vntifkey="21"
                     /label=L-ITR
     misc_feature    2232..2372
                     /vntifkey="21"
                     /label=R-ITR
     rep_origin      2464..2770
                     /vntifkey="33"
                     /label=f1\Origin
     misc_feature    3289..4146
                     /vntifkey="21"
                     /label=AmpR
     rep_origin      4297..4964
                     /vntifkey="33"
                     /label=pUC\ori
     primer          complement(2114..2133)
                     /vntifkey="27"
                     /label=BGH-R
     misc_feature    2000..2203
                     /vntifkey="21"
                     /label=BGH-pA
     misc_feature    1741..1986
                     /vntifkey="21"
                     /label=WPRE3
     misc_feature    1741..1900
                     /vntifkey="21"
                     /label=gamma
     misc_feature    1906..1986
                     /vntifkey="21"
                     /label=alpha
     misc_feature    complement(1854..1873)
                     /vntifkey="21"
                     /label=WPRE3-R\(JT)
     misc_feature    894..946
                     /vntifkey="21"
                     /label=Beta\Globin\minimal\promoter
     misc_feature    903..908
                     /vntifkey="21"
                     /label=TATA\box
     misc_feature    935..935
                     /vntifkey="21"
                     /label=transcriptional\start
     misc_feature    987..1706
                     /vntifkey="21"
                     /label=SYFP2
     misc_feature    complement(909..929)
                     /vntifkey="21"
                     /label=minBG-PCR-R
     misc_feature    156..887
                     /vntifkey="21"
                     /label=eHGT_486m(3xCore)
BASE COUNT     1193 a      1296 c      1221 g      1259 t 
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 
      121 actccatcac taggggttcc tgcggccgca cgcgtaaaat caaatcagtt gcaaaatcca 
      181 tacagtcttc cagtttctca gctgtggaaa ctttgtgtct cttcttttcc accaaaacag 
      241 ctatctcgtc tttcaagtcc ttgctgaaaa aaaaaagtgt taaatttttc tcaatttaga 
      301 cttccagcaa aacagattct gcttgttgaa aggcattggt gttcatgagt aagtgagttt 
      361 ccattttatt tcttcacaaa taagttacat gaagtactta aaatcaaatc agttgcaaaa 
      421 tccatacagt cttccagttt ctcagctgtg gaaactttgt gtctcttctt ttccaccaaa 
      481 acagctatct cgtctttcaa gtccttgctg aaaaaaaaaa gtgttaaatt tttctcaatt 
      541 tagacttcca gcaaaacaga ttctgcttgt tgaaaggcat tggtgttcat gagtaagtga 
      601 gtttccattt tatttcttca caaataagtt acatgaagta cttaaaatca aatcagttgc 
      661 aaaatccata cagtcttcca gtttctcagc tgtggaaact ttgtgtctct tcttttccac 
      721 caaaacagct atctcgtctt tcaagtcctt gctgaaaaaa aaaagtgtta aatttttctc 
      781 aatttagact tccagcaaaa cagattctgc ttgttgaaag gcattggtgt tcatgagtaa 
      841 gtgagtttcc attttatttc ttcacaaata agttacatga agtacttgag ctcgggctgg 
      901 gcataaaagt cagggcagag ccatctattg cttacatttg cttctgggat ccagatcttt 
      961 cgaagctagc gctaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg 
     1021 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg 
     1081 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagctgatc tgcaccaccg 
     1141 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gggctacggc gtgcagtgct 
     1201 tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag 
     1261 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg 
     1321 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca 
     1381 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct 
     1441 atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcaagatc cgccacaaca 
     1501 tcgaggacgg cggcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg 
     1561 gccccgtgct gctgcccgac aaccactacc tgagctacca gtccaagctg agcaaagacc 
     1621 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc 
     1681 tcggcatgga cgagctgtac aagtaagtcg acggcgcgcc gcggccgcga attcgatatc 
     1741 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg 
     1801 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc 
     1861 gtatggcttt cattttctcc tccttgtata aatcctggtt agttcttgcc acggcggaac 
     1921 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt 
     1981 ccgtggctcg agagatcttc gactgtgcct tctagttgcc agccatctgt tgtttgcccc 
     2041 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat 
     2101 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg 
     2161 caggacagca agggggagga ttgggaagac aatagcaggc atgagatctc acgtgcggac 
     2221 cgagcggccg caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc 
     2281 gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc 
     2341 agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt ttctccttac 
     2401 gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc 
     2461 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc 
     2521 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt 
     2581 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac 
     2641 ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag 
     2701 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa 
     2761 actggaacaa cactcaaccc tatctcgggc tattcttttg atttataagg gattttgccg 
     2821 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac 
     2881 aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca 
     2941 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg 
     3001 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg 
     3061 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta 
     3121 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat 
     3181 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg 
     3241 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa 
     3301 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac 
     3361 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac 
     3421 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt 
     3481 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc 
     3541 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca 
     3601 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc 
     3661 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag 
     3721 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa 
     3781 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg 
     3841 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa 
     3901 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg 
     3961 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt 
     4021 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt 
     4081 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag 
     4141 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat 
     4201 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct 
     4261 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct 
     4321 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca 
     4381 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc 
     4441 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc 
     4501 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct 
     4561 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag 
     4621 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc 
     4681 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg 
     4741 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag 
     4801 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt 
     4861 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac 
     4921 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgt 
//
