LOCUS       CN2786_pAAV-3xcore_eHGT_121h-minBG-SYFP2-WPRE3-BGHpA        5137 bp    DNA     circular UNA 26-AUG-2020
DEFINITION  .
ACCESSION   urn.local...ao-cc01kdg
VERSION     urn.local...ao-cc01kdg
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     ##Author-START##
            Name :: Jonathan Ting
            ##Author-END##
            ##Comment-START##
            Comment :: rAAV-hPTenh(FAM84b)-minBG-SYFP2-WPRE3-BGHpA
                       clones 2,3,4 all good
                       clone 1 low yield
            ##Comment-END##
            ##Imported_From-START##
            Path     :: C:\VNTI Database
            Filename :: _exdata.ma4
            ##Imported_From-END##
FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /label="L-ITR"
     misc_feature    156..1055
                     /Original_Bases="GGTACCCTAAATAAAGATGGCTTTTTAGTATTAAAAGTGGA
                     AGAAAATTACAGGTAATTATCTTTGACGGTAAAAACGCTGTAATCAGCGGGCTACATG
                     AAAAATTACTCTAATTATGGCTGCATTTAAGAGAATGGCTAAATAAAGATGGCTTTTT
                     AGTATTAAAAGTGGAAGAAAATTACAGGTAATTATCTTTGACGGTAAAAACGCTGTAA
                     TCAGCGGGCTACATGAAAAATTACTCTAATTATGGCTGCATTTAAGAGAATGGCTAAA
                     TAAAGATGGCTTTTTAGTATTAAAAGTGGAAGAAAATTACAGGTAATTATCTTTGACG
                     GTAAAAACGCTGTAATCAGCGGGCTACATGAAAAATTACTCTAATTATGGCTGCATTT
                     AAGAGAATG"
                     /label="GGTACCCTAAATAAAGATGGCTTTTTAGTATTAAAAGTGGAAGAAAATTA
                     CAGGTAATTATCTTTGACGGTAAAAACGCTGTAATCAGCGGGCTACATGAAAAATTAC
                     TCTAATTATGGCTGCATTTAAGAGAATGGCTAAATAAAGATGGCTTTTTAGTATTAAA
                     AGTGGAAGAAAATTACAGGTAATTATCTTTGACGGTAAAAACGCTGTAATCAGCGGGC
                     TACATGAAAAATTACTCTAATTATGGCTGCATTTAAGAGAATGGCTAAATAAAGATGG
                     CTTTTTAGTATTAAAAGTGGAAGAAAATTACAGGTAATTATCTTTGACGGTAAAAACG
                     CTGTAATCAGCGGGCTACATGAAAAATTACTCTAATTATGGCTGCATTTAAGAGAATG
                     "
                     /note="Geneious type: Editing History Replacement"
     misc_feature    156..455
                     /label="eHGT_121h"
     misc_feature    156..1055
                     /created_by="ximena.opitzaraya"
                     /label="3xcore_eHGT_121h"
     misc_feature    456..755
                     /label="eHGT_121h"
     misc_feature    756..1055
                     /label="eHGT_121h"
     misc_feature    1062..1114
                     /label="Beta Globin minimal promoter"
     misc_feature    1071..1076
                     /label="TATA box"
     misc_feature    complement(1077..1097)
                     /label="minBG-PCR-R"
     misc_feature    1103
                     /label="transcriptional start"
     misc_feature    1155..1874
                     /label="SYFP2"
     misc_feature    1909..2154
                     /label="WPRE3"
     misc_feature    1909..2068
                     /label="gamma"
     misc_feature    complement(2022..2041)
                     /label="WPRE3-R (JT)"
     misc_feature    2074..2154
                     /label="alpha"
     misc_feature    2168..2371
                     /label="BGH-pA"
     primer          complement(2282..2301)
                     /label="BGH-R"
     misc_feature    2400..2540
                     /label="R-ITR"
     rep_origin      2632..2938
                     /label="f1 Origin"
     misc_feature    3457..4314
                     /label="AmpR"
     rep_origin      4465..5132
                     /label="pUC ori"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtctatc tggtttataa ttagaagtga
      181 aatgatttta aatggcttcc acatggctta gttttccaag cagttccagt tacatagaac
      241 ttcatataaa cagataatga gaggctagag ttggagagca cagttcctgc ttccaaattg
      301 gcttgaagtg gggacatctg ctctgcatga agcctgggtc aggctgcccc tctcatctgt
      361 gacctcctgt ggtcgggagg gacttgctct ggattatata aaggcttcac aagagtactg
      421 gaagcgggga cggaatcaac ttgagcaaaa aacctctatc tggtttataa ttagaagtga
      481 aatgatttta aatggcttcc acatggctta gttttccaag cagttccagt tacatagaac
      541 ttcatataaa cagataatga gaggctagag ttggagagca cagttcctgc ttccaaattg
      601 gcttgaagtg gggacatctg ctctgcatga agcctgggtc aggctgcccc tctcatctgt
      661 gacctcctgt ggtcgggagg gacttgctct ggattatata aaggcttcac aagagtactg
      721 gaagcgggga cggaatcaac ttgagcaaaa aacctctatc tggtttataa ttagaagtga
      781 aatgatttta aatggcttcc acatggctta gttttccaag cagttccagt tacatagaac
      841 ttcatataaa cagataatga gaggctagag ttggagagca cagttcctgc ttccaaattg
      901 gcttgaagtg gggacatctg ctctgcatga agcctgggtc aggctgcccc tctcatctgt
      961 gacctcctgt ggtcgggagg gacttgctct ggattatata aaggcttcac aagagtactg
     1021 gaagcgggga cggaatcaac ttgagcaaaa aacctgagct cgggctgggc ataaaagtca
     1081 gggcagagcc atctattgct tacatttgct tctgggatcc agatctttcg aagctagcgc
     1141 taccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     1201 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     1261 gcgatgccac ctacggcaag ctgaccctga agctgatctg caccaccggc aagctgcccg
     1321 tgccctggcc caccctcgtg accaccctgg gctacggcgt gcagtgcttc gcccgctacc
     1381 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     1441 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     1501 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     1561 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcaccgccg
     1621 acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc gaggacggcg
     1681 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     1741 tgcccgacaa ccactacctg agctaccagt ccaagctgag caaagacccc aacgagaagc
     1801 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     1861 agctgtacaa gtaagtcgac ggcgcgccgc ggccgcgaat tcgatatcat aatcaacctc
     1921 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     1981 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     2041 ttttctcctc cttgtataaa tcctggttag ttcttgccac ggcggaactc atcgccgcct
     2101 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggctcgag
     2161 agatcttcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     2221 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     2281 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     2341 ggggaggatt gggaagacaa tagcaggcat gagatctcac gtgcggaccg agcggccgca
     2401 ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc
     2461 cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg
     2521 agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     2581 tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc
     2641 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     2701 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     2761 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     2821 aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     2881 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     2941 ctcaacccta tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     3001 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     3061 tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     3121 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     3181 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     3241 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     3301 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     3361 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     3421 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     3481 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     3541 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     3601 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     3661 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     3721 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     3781 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     3841 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     3901 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     3961 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     4021 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     4081 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     4141 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     4201 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     4261 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     4321 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     4381 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     4441 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     4501 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     4561 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     4621 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     4681 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     4741 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     4801 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     4861 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     4921 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     4981 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     5041 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     5101 cggttcctgg ccttttgctg gccttttgct cacatgt
//
