LOCUS       CN2844_rAAV-eHGT_664m-minBglobin-SYFP2-WPRE3-BGHpA        5027 bp    DNA     circular UNA 28-AUG-2020
DEFINITION  Gibson/Homology Cloning ligation product.
ACCESSION   urn.local...3y8-ccal1ju
VERSION     urn.local...3y8-ccal1ju
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /vntifkey=21
                     /locus_tag="L-ITR"
                     /ApEinfo_label="L-ITR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="L-ITR"
     misc_feature    complement(137..969)
                     /Source="referenceToDocument: urn:local:.:3ox-ccakua6"
                     /label="664m-RMSYFP2seqR.ab1 (concatenated sequence 2)
                     (reversed)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    136^137
                     /Original_Bases="NGCGNNNCNNGNNNGNNGGAGTGGCCAACTCCATCACTAGG
                     GG"
                     /label="NGCGNNNCNNGNNNGNNGGAGTGGCCAACTCCATCACTAGGGG"
                     /note="Geneious type: Editing History Deletion"
     ligation        137..154
                     /label="Ligation"
     primer_bind     137..155
                     /%GC="73.7"
                     /Hairpin_Tm="60.9"
                     /Self_Dimer_Tm="46.9"
                     /Tm="70.6"
                     /Pair_Dimer_Tm="None"
                     /annotation_group="1479674966058356e348cd71-b704-4f3c-a8f5
                     -10daf13542eb"
                     /created_by="primer3"
                     /Sequence="TTCCTGCGGCCGCACGCGT"
                     /Mismatches=0
                     /Product_Size=833
                     /#_Off-target_Sites=0
                     /Off-target_Template="664m-RMSYFP2seqR.ab1 (reversed)"
                     /label="Gibson Assembly into CN1244 F'"
     misc_feature    156..945
                     /Color="#ffff55"
                     /created_by="refugio.martinez"
                     /label="eHGT_664m"
     primer_bind     complement(946..969)
                     /%GC="58.3"
                     /Hairpin_Tm="51.4"
                     /Self_Dimer_Tm="2.7"
                     /Tm="65.8"
                     /Pair_Dimer_Tm="None"
                     /annotation_group="1479674966058356e348cd71-b704-4f3c-a8f5
                     -10daf13542eb"
                     /created_by="primer3"
                     /Sequence="GACTTTTATGCCCAGCCCGAGCTC"
                     /Mismatches=0
                     /Product_Size=833
                     /#_Off-target_Sites=0
                     /Off-target_Template="664m-RMSYFP2seqR.ab1 (reversed)"
                     /label="Gibson Assembly into CN1244 R'"
     misc_feature    951..154
                     /Source="referenceToDocument: urn:local:.:3by-b7vj7bw"
                     /label="CN1244_pAAV Linearized (digested by SacI, MluI)
                     Fragment 3 (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     ligation        951..969
                     /label="Ligation"
     misc_feature    952..1004
                     /vntifkey=21
                     /locus_tag="Beta\Globin\minimal\promoter"
                     /ApEinfo_label="Beta\Globin\minimal\promoter"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Beta\Globin\minimal\promoter"
     misc_feature    961..966
                     /vntifkey=21
                     /locus_tag="TATA\box"
                     /ApEinfo_label="TATA\box"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="TATA\box"
     misc_feature    969^970
                     /Original_Bases="AGGGCAGAGCCATCTATTGCTTACATTTGCTTNNNNANCCA
                     GANNTNNNNNNNNNNNNNNNN"
                     /label="AGGGCAGAGCCATCTATTGCTTACATTTGCTTNNNNANCCAGANNTNNNN
                     NNNNNNNNNNNN"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    993
                     /vntifkey=21
                     /locus_tag="transcriptional\start"
                     /ApEinfo_label="transcriptional\start"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="transcriptional\start"
     misc_feature    1045..1764
                     /vntifkey=21
                     /locus_tag="SYFP2"
                     /ApEinfo_label="SYFP2"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="SYFP2"
     misc_feature    1799..2044
                     /vntifkey=21
                     /locus_tag="WPRE3"
                     /ApEinfo_label="WPRE3"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="WPRE3"
     misc_feature    2058..2261
                     /vntifkey=21
                     /locus_tag="BGH-pA"
                     /ApEinfo_label="BGH-pA"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="BGH-pA"
     primer          complement(2172..2191)
                     /vntifkey=27
                     /locus_tag="BGH-R"
                     /ApEinfo_label="BGH-R"
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#80ff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="BGH-R"
     misc_feature    2290..2430
                     /vntifkey=21
                     /locus_tag="R-ITR"
                     /ApEinfo_label="R-ITR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="R-ITR"
     rep_origin      2522..2828
                     /vntifkey=33
                     /locus_tag="f1\Origin"
                     /ApEinfo_label="f1\Origin"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="f1\Origin"
     misc_feature    3347..4204
                     /vntifkey=21
                     /locus_tag="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="AmpR"
     rep_origin      4355..5022
                     /vntifkey=33
                     /locus_tag="pUC\ori"
                     /ApEinfo_label="pUC\ori"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="pUC\ori"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtttgaa cagattgcat gtttccttta
      181 atatgaaata tcttaaatag gtaaacccat aaacagaaag cattccagtg tttgccagga
      241 atggggaagc gggaactggg agtcactgtg taatgggagt ggagggatac aaacattgga
      301 aaacatggga gaagtaaacg attaatggat atgtttcatg ccaccgaatt atataccaga
      361 aaatgattaa aatagttagc atttatatga tgtttattct gccacaacaa aaataattga
      421 agagagaggg agagggagag agaatgtcaa tggtattcat ggacctggcc cctagcaagt
      481 gtgatgggaa ctgatgtttc cataggaatg ttacctaaaa ctctcatgtg ccagacagct
      541 tttcacactg tgacaagatt tctgagaaaa cgatttcagg gaagatgggc ttcctggctt
      601 taggcctgag atggaaaaga acatcatggt ggtagcctgt gcaggaggag gaagctgctc
      661 cccacacaca ttcagcagag agagagagag agagatgagg ggtgggcagg gaggaagacc
      721 aagccaaccc aggctggcag cttgccttcc cttctttatc tgactcctca gcctaaggga
      781 tggcaccacc aatactcatg gtagatttca ccttcaacca ctgtagcaca tgtcagttgt
      841 ctctgactgt cagatgtctg tctcacgtga ttgtgccgtc acatgcacat ccagaagagt
      901 catttcttcg tcttctaggt atctttcaat ccagtccagg tcaccgagct cgggctgggc
      961 ataaaagtca gggcagagcc atctattgct tacatttgct tctgggatcc agatctttcg
     1021 aagctagcgc taccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg
     1081 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     1141 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agctgatctg caccaccggc
     1201 aagctgcccg tgccctggcc caccctcgtg accaccctgg gctacggcgt gcagtgcttc
     1261 gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     1321 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     1381 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     1441 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     1501 atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc
     1561 gaggacggcg gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     1621 cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccaagctgag caaagacccc
     1681 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     1741 ggcatggacg agctgtacaa gtaagtcgac ggcgcgccgc ggccgcgaat tcgatatcat
     1801 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     1861 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     1921 atggctttca ttttctcctc cttgtataaa tcctggttag ttcttgccac ggcggaactc
     1981 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     2041 gtggctcgag agatcttcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     2101 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     2161 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     2221 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gagatctcac gtgcggaccg
     2281 agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc
     2341 tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag
     2401 tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc
     2461 atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg
     2521 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     2581 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     2641 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     2701 cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac
     2761 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     2821 tggaacaaca ctcaacccta tctcgggcta ttcttttgat ttataaggga ttttgccgat
     2881 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     2941 aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata
     3001 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     3061 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     3121 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
     3181 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     3241 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     3301 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     3361 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     3421 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     3481 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     3541 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     3601 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     3661 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     3721 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     3781 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     3841 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     3901 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     3961 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     4021 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     4081 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     4141 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     4201 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     4261 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     4321 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     4381 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     4441 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     4501 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     4561 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     4621 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     4681 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     4741 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     4801 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     4861 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     4921 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     4981 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgt
//
