LOCUS       CN2860_rAAV-eHGT_700m-minBglobin-SYFP2-WPRE3-BGHpA        4835 bp    DNA     circular UNA 28-AUG-2020
DEFINITION  Gibson/Homology Cloning ligation product.
ACCESSION   urn.local...3x2-ccal1j1
VERSION     urn.local...3x2-ccal1j1
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /vntifkey=21
                     /locus_tag="L-ITR"
                     /ApEinfo_label="L-ITR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="L-ITR"
     misc_feature    complement(137..777)
                     /Source="referenceToDocument: urn:local:.:3ox-ccakua6"
                     /label="m700-RMSYFP2seqR.ab1 (concatenated sequence 2)
                     (reversed)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    136^137
                     /Original_Bases="GAGCGCGCAGAGAGGGAGTGGCCANNTCCATCACTAGGGG"
                     /label="GAGCGCGCAGAGAGGGAGTGGCCANNTCCATCACTAGGGG"
                     /note="Geneious type: Editing History Deletion"
     ligation        137..154
                     /label="Ligation"
     primer_bind     137..155
                     /%GC="73.7"
                     /Hairpin_Tm="60.9"
                     /Self_Dimer_Tm="46.9"
                     /Tm="70.6"
                     /Pair_Dimer_Tm="None"
                     /annotation_group="147966971202425185ee57c2-4fc1-4fa3-ba15
                     -5334f77b4114"
                     /created_by="primer3"
                     /Sequence="TTCCTGCGGCCGCACGCGT"
                     /Mismatches=0
                     /Product_Size=641
                     /#_Off-target_Sites=0
                     /Off-target_Template="m700-RMSYFP2seqR.ab1 (reversed)"
                     /label="Gibson Assembly into CN1244 F'"
     misc_feature    156..753
                     /Color="#ffff55"
                     /created_by="refugio.martinez"
                     /label="eHGT_700m"
     primer_bind     complement(754..777)
                     /%GC="58.3"
                     /Hairpin_Tm="51.4"
                     /Self_Dimer_Tm="2.7"
                     /Tm="65.8"
                     /Pair_Dimer_Tm="None"
                     /annotation_group="147966971202425185ee57c2-4fc1-4fa3-ba15
                     -5334f77b4114"
                     /created_by="primer3"
                     /Sequence="GACTTTTATGCCCAGCCCGAGCTC"
                     /Mismatches=0
                     /Product_Size=641
                     /#_Off-target_Sites=0
                     /Off-target_Template="m700-RMSYFP2seqR.ab1 (reversed)"
                     /label="Gibson Assembly into CN1244 R'"
     misc_feature    759..154
                     /Source="referenceToDocument: urn:local:.:3by-b7vj7bw"
                     /label="CN1244_pAAV Linearized (digested by SacI, MluI)
                     Fragment 3 (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     ligation        759..777
                     /label="Ligation"
     misc_feature    760..812
                     /vntifkey=21
                     /locus_tag="Beta\Globin\minimal\promoter"
                     /ApEinfo_label="Beta\Globin\minimal\promoter"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Beta\Globin\minimal\promoter"
     misc_feature    769..774
                     /vntifkey=21
                     /locus_tag="TATA\box"
                     /ApEinfo_label="TATA\box"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="TATA\box"
     misc_feature    777^778
                     /Original_Bases="AGGGCAGAGCCATCTATTGCTTACATTTGCNNNNNNNNNNN
                     NNNNNNNNTNNNNNNNNNNNNNNNNNN"
                     /label="AGGGCAGAGCCATCTATTGCTTACATTTGCNNNNNNNNNNNNNNNNNNNT
                     NNNNNNNNNNNNNNNNNN"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    801
                     /vntifkey=21
                     /locus_tag="transcriptional\start"
                     /ApEinfo_label="transcriptional\start"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="transcriptional\start"
     misc_feature    853..1572
                     /vntifkey=21
                     /locus_tag="SYFP2"
                     /ApEinfo_label="SYFP2"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="SYFP2"
     misc_feature    1607..1852
                     /vntifkey=21
                     /locus_tag="WPRE3"
                     /ApEinfo_label="WPRE3"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="WPRE3"
     misc_feature    1866..2069
                     /vntifkey=21
                     /locus_tag="BGH-pA"
                     /ApEinfo_label="BGH-pA"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="BGH-pA"
     primer          complement(1980..1999)
                     /vntifkey=27
                     /locus_tag="BGH-R"
                     /ApEinfo_label="BGH-R"
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#80ff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="BGH-R"
     misc_feature    2098..2238
                     /vntifkey=21
                     /locus_tag="R-ITR"
                     /ApEinfo_label="R-ITR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="R-ITR"
     rep_origin      2330..2636
                     /vntifkey=33
                     /locus_tag="f1\Origin"
                     /ApEinfo_label="f1\Origin"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="f1\Origin"
     misc_feature    3155..4012
                     /vntifkey=21
                     /locus_tag="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="AmpR"
     rep_origin      4163..4830
                     /vntifkey=33
                     /locus_tag="pUC\ori"
                     /ApEinfo_label="pUC\ori"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="pUC\ori"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtttggc tttccttcca agttgtattt
      181 tggtgaagtt tgactcctgg aatctcactt ctgcacttta gattagcata ctttaaaaag
      241 gaacattaca gtacaatgga aaagtaaaga tctgggtttt atctcagctt tagttagtat
      301 ttataggaag catggattac ataaagagat gacattattt cagtctgaaa ttgatttgta
      361 ctatacgaca tttcattttg tttgtgcaca ttgattagat aattcctcaa atcagtttga
      421 aaatttcatc tctgttcata gctgaagata accaccaaat tgcttttaac taatttacat
      481 aataagtata gtagaggttt attctatata aagtatcaaa tataaatatt catctcaaat
      541 tctaaaggat ttagcaatgt aaattactta tgaattacat acacatttgt atttaaatct
      601 tttttcactt ttattaaaaa tagatccttt tctcatacaa tatatcctga ttgcagttcc
      661 ctcccctcta ctcctcccag ttccttccca cctccctttc catctggatc tctctcctat
      721 taaaggttgg acagaagaga tggcactgca tgtgagctcg ggctgggcat aaaagtcagg
      781 gcagagccat ctattgctta catttgcttc tgggatccag atctttcgaa gctagcgcta
      841 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      901 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      961 gatgccacct acggcaagct gaccctgaag ctgatctgca ccaccggcaa gctgcccgtg
     1021 ccctggccca ccctcgtgac caccctgggc tacggcgtgc agtgcttcgc ccgctacccc
     1081 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     1141 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     1201 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1261 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat caccgccgac
     1321 aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcggc
     1381 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1441 cccgacaacc actacctgag ctaccagtcc aagctgagca aagaccccaa cgagaagcgc
     1501 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1561 ctgtacaagt aagtcgacgg cgcgccgcgg ccgcgaattc gatatcataa tcaacctctg
     1621 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     1681 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     1741 ttctcctcct tgtataaatc ctggttagtt cttgccacgg cggaactcat cgccgcctgc
     1801 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggctcgagag
     1861 atcttcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     1921 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     1981 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     2041 ggaggattgg gaagacaata gcaggcatga gatctcacgt gcggaccgag cggccgcagg
     2101 aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg
     2161 ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag
     2221 cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     2281 tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc
     2341 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     2401 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     2461 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     2521 acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     2581 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     2641 caaccctatc tcgggctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     2701 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
     2761 tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     2821 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     2881 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     2941 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     3001 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     3061 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     3121 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     3181 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     3241 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     3301 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     3361 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     3421 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     3481 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     3541 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     3601 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     3661 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     3721 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     3781 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     3841 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     3901 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     3961 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     4021 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     4081 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     4141 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     4201 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     4261 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     4321 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     4381 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     4441 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     4501 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     4561 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     4621 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     4681 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     4741 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     4801 gttcctggcc ttttgctggc cttttgctca catgt
//
