LOCUS       CN2924_pAAV-3xcore2_eHGT_078h-minBglobin-SYFP2-WPRE3-BGHpA        4867 bp    DNA     circular UNA 02-OCT-2020
DEFINITION  .
ACCESSION   urn.local...hs-chbeq6i
VERSION     urn.local...hs-chbeq6i
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     ##Comment-START##
            Comment :: rAAV-ESYN-SYFP2-WPRE3-BGHpA
                       clone #8-1 correct , 8-2 backup
            ##Comment-END##
            ##Imported_From-START##
            Path     :: C:\VNTI Database
            Filename :: _exdata.ma4
            ##Imported_From-END##
FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /label="L-ITR"
     misc_feature    156..791
                     /Original_Bases="TAGTCTGCCTCAGGTACACACTGAGAAACTGCTTTAATGTA
                     ACCTGACCCACGGTTATTAGTGAAAATATCACTTTTGTTGTTACCTTATTCCCAACAA
                     ATTCATTTCTGCTTTAATGGAAAAGATCCGGGTTCACACTAATCAGGCCCAACGGAAG
                     GCCATATTAGCAATTTGGCAGGTACCCGAGGGCCATACCTAATCTGCATAAAATGTAG
                     TCTGCCTCAGGTACACACTGAGAAACTGCTTTAATGTAACCTGACCCACGGTTATTAG
                     TGAAAATATCACTTTTGTTGTTACCTTATTCCCAACAAATTCATTTCTGCTTTAATGG
                     AAAAGATCCGGGTTCACACTAATCAGGCCCAACGGAAGGCCATATTAGCAATTTGGCA
                     GGTACCCGAGGGCCATACCTAATCTGCATAAAATGTAGTCTGCCTCAGGTACACACTG
                     AGAAACTGCTTTAATGTAACCTGACCCACGGTTATTAGTGAAAATATCACTTTTGTTG
                     TTACCTTATTCCCAACAAATTCATTTCTGCTTTAATGGAAAAGATCCGGGTTCACACT
                     AATCAGGCCCAACGGAAGGCCATATTAGCAATTTGGCAGGTACCCGAGGGCCATACCT
                     AATCTGCATAAAATG"
                     /label="TAGTCTGCCTCAGGTACACACTGAGAAACTGCTTTAATGTAACCTGACCC
                     ACGGTTATTAGTGAAAATATCACTTTTGTTGTTACCTTATTCCCAACAAATTCATTTC
                     TGCTTTAATGGAAAAGATCCGGGTTCACACTAATCAGGCCCAACGGAAGGCCATATTA
                     GCAATTTGGCAGGTACCCGAGGGCCATACCTAATCTGCATAAAATGTAGTCTGCCTCA
                     GGTACACACTGAGAAACTGCTTTAATGTAACCTGACCCACGGTTATTAGTGAAAATAT
                     CACTTTTGTTGTTACCTTATTCCCAACAAATTCATTTCTGCTTTAATGGAAAAGATCC
                     GGGTTCACACTAATCAGGCCCAACGGAAGGCCATATTAGCAATTTGGCAGGTACCCGA
                     GGGCCATACCTAATCTGCATAAAATGTAGTCTGCCTCAGGTACACACTGAGAAACTGC
                     TTTAATGTAACCTGACCCACGGTTATTAGTGAAAATATCACTTTTGTTGTTACCTTAT
                     TCCCAACAAATTCATTTCTGCTTTAATGGAAAAGATCCGGGTTCACACTAATCAGGCC
                     CAACGGAAGGCCATATTAGCAATTTGGCAGGTACCCGAGGGCCATACCTAATCTGCAT
                     AAAATG"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    156..791
                     /created_by="ximena.opitzaraya"
                     /label="3xcore2_eHGT_078h"
     misc_feature    798..850
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Beta Globin minimal promoter"
     misc_feature    807..812
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="TATA box"
     misc_feature    839
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="transcriptional start"
     misc_feature    891..1610
                     /label="SYFP2"
     primer_bind     937..957
                     /label="EGFP-N-R"
     misc_feature    1645..1890
                     /label="WPRE3"
     misc_feature    1904..2107
                     /label="BGH-pA"
     primer          complement(2018..2037)
                     /label="BGH-R"
     misc_feature    2130..2270
                     /label="R-ITR"
     rep_origin      2362..2668
                     /label="f1 Origin"
     misc_feature    3187..4044
                     /label="AmpR"
     rep_origin      4195..4862
                     /label="pUC ori"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtattag caatttggca ggtacccgag
      181 ggccatacct aatctgcata aaatgaagca gattgcaacc gccctcatct tttttatttt
      241 taaactggtt tttgaagcag agcataaaat ctcagaggga gagacagaag atgctagtgc
      301 atacattttc cttcatgcct ttattttcat tctttttgca caaaccatct tcctgaatgg
      361 ctgtttaatt agcaatttgg caggtacccg agggccatac ctaatctgca taaaatgaag
      421 cagattgcaa ccgccctcat cttttttatt tttaaactgg tttttgaagc agagcataaa
      481 atctcagagg gagagacaga agatgctagt gcatacattt tccttcatgc ctttattttc
      541 attctttttg cacaaaccat cttcctgaat ggctgtttaa ttagcaattt ggcaggtacc
      601 cgagggccat acctaatctg cataaaatga agcagattgc aaccgccctc atctttttta
      661 tttttaaact ggtttttgaa gcagagcata aaatctcaga gggagagaca gaagatgcta
      721 gtgcatacat tttccttcat gcctttattt tcattctttt tgcacaaacc atcttcctga
      781 atggctgttt agagctcggg ctgggcataa aagtcagggc agagccatct attgcttaca
      841 tttgcttctg ggatccagat ctttcgaagc tagcgctacc ggtcgccacc atggtgagca
      901 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
      961 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     1021 ccctgaagct gatctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     1081 ccctgggcta cggcgtgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact
     1141 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     1201 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     1261 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     1321 acaactacaa cagccacaac gtctatatca ccgccgacaa gcagaagaac ggcatcaagg
     1381 ccaacttcaa gatccgccac aacatcgagg acggcggcgt gcagctcgcc gaccactacc
     1441 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct
     1501 accagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     1561 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa gtcgacggcg
     1621 cgccgcggcc gcgaattcga tatcataatc aacctctgga ttacaaaatt tgtgaaagat
     1681 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     1741 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     1801 ggttagttct tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     1861 ctcggctgtt gggcactgac aattccgtgg ctcgagagat cttcgactgt gccttctagt
     1921 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     1981 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     2041 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     2101 aggcatgcac gtgcggaccg agcggccgca ggaaccccta gtgatggagt tggccactcc
     2161 ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg
     2221 ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat
     2281 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca
     2341 tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     2401 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     2461 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     2521 tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt
     2581 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     2641 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcgggcta ttcttttgat
     2701 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     2761 tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca
     2821 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     2881 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     2941 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     3001 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     3061 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     3121 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     3181 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     3241 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     3301 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     3361 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     3421 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     3481 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     3541 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     3601 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     3661 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     3721 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     3781 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     3841 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     3901 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     3961 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     4021 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     4081 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     4141 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     4201 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     4261 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     4321 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     4381 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     4441 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     4501 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     4561 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     4621 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     4681 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     4741 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     4801 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     4861 cacatgt
//
