LOCUS       CN3554_pAAV-3xcore2_eHGT_017h-minBG-SYFP2-WPRE3-BGHpA        4714 bp    DNA     circular UNA 26-JUL-2021
DEFINITION  .
ACCESSION   urn.local...82-dnr4b2u
VERSION     urn.local...82-dnr4b2u
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     ##Author-START##
            Name :: Jonathan Ting
            ##Author-END##
            ##Comment-START##
            Comment :: rAAV-hPTenh(FAM84b)-minBG-SYFP2-WPRE3-BGHpA
                       clones 2,3,4 all good
                       clone 1 low yield
            ##Comment-END##
            ##Imported_From-START##
            Path     :: C:\VNTI Database
            Filename :: _exdata.ma4
            ##Imported_From-END##
FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /label="L-ITR"
     misc_feature    156..632
                     /Original_Bases="GGTACCCTAAATAAAGATGGCTTTTTAGTATTAAAAGTGGA
                     AGAAAATTACAGGTAATTATCTTTGACGGTAAAAACGCTGTAATCAGCGGGCTACATG
                     AAAAATTACTCTAATTATGGCTGCATTTAAGAGAATGGCTAAATAAAGATGGCTTTTT
                     AGTATTAAAAGTGGAAGAAAATTACAGGTAATTATCTTTGACGGTAAAAACGCTGTAA
                     TCAGCGGGCTACATGAAAAATTACTCTAATTATGGCTGCATTTAAGAGAATGGCTAAA
                     TAAAGATGGCTTTTTAGTATTAAAAGTGGAAGAAAATTACAGGTAATTATCTTTGACG
                     GTAAAAACGCTGTAATCAGCGGGCTACATGAAAAATTACTCTAATTATGGCTGCATTT
                     AAGAGAATG"
                     /label="GGTACCCTAAATAAAGATGGCTTTTTAGTATTAAAAGTGGAAGAAAATTA
                     CAGGTAATTATCTTTGACGGTAAAAACGCTGTAATCAGCGGGCTACATGAAAAATTAC
                     TCTAATTATGGCTGCATTTAAGAGAATGGCTAAATAAAGATGGCTTTTTAGTATTAAA
                     AGTGGAAGAAAATTACAGGTAATTATCTTTGACGGTAAAAACGCTGTAATCAGCGGGC
                     TACATGAAAAATTACTCTAATTATGGCTGCATTTAAGAGAATGGCTAAATAAAGATGG
                     CTTTTTAGTATTAAAAGTGGAAGAAAATTACAGGTAATTATCTTTGACGGTAAAAACG
                     CTGTAATCAGCGGGCTACATGAAAAATTACTCTAATTATGGCTGCATTTAAGAGAATG
                     "
                     /note="Geneious type: Editing History Replacement"
     misc_feature    156..632
                     /created_by="ximena.opitzaraya"
                     /label="3xcore2_eHGT_017h"
     misc_feature    639..691
                     /label="Beta Globin minimal promoter"
     misc_feature    648..653
                     /label="TATA box"
     misc_feature    complement(654..674)
                     /label="minBG-PCR-R"
     misc_feature    680
                     /label="transcriptional start"
     misc_feature    732..1451
                     /label="SYFP2"
     misc_feature    1486..1731
                     /label="WPRE3"
     misc_feature    1486..1645
                     /label="gamma"
     misc_feature    complement(1599..1618)
                     /label="WPRE3-R (JT)"
     misc_feature    1651..1731
                     /label="alpha"
     misc_feature    1745..1948
                     /label="BGH-pA"
     primer          complement(1859..1878)
                     /label="BGH-R"
     misc_feature    1977..2117
                     /label="R-ITR"
     rep_origin      2209..2515
                     /label="f1 Origin"
     misc_feature    3034..3891
                     /label="AmpR"
     rep_origin      4042..4709
                     /label="pUC ori"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtagcag gcgcccctcc tccccacctc
      181 gctaaacaat cattgcacaa aatatgcaaa tggtataatt actgttattt gttttgctga
      241 tataagtgtt tgaaatgcaa atgtcaagtt tgggcgcctt catttttcca accctctcac
      301 ccggacattt gcaaagcagg cgcccctcct ccccacctcg ctaaacaatc attgcacaaa
      361 atatgcaaat ggtataatta ctgttatttg ttttgctgat ataagtgttt gaaatgcaaa
      421 tgtcaagttt gggcgccttc atttttccaa ccctctcacc cggacatttg caaagcaggc
      481 gcccctcctc cccacctcgc taaacaatca ttgcacaaaa tatgcaaatg gtataattac
      541 tgttatttgt tttgctgata taagtgtttg aaatgcaaat gtcaagtttg ggcgccttca
      601 tttttccaac cctctcaccc ggacatttgc aagagctcgg gctgggcata aaagtcaggg
      661 cagagccatc tattgcttac atttgcttct gggatccaga tctttcgaag ctagcgctac
      721 cggtcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
      781 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
      841 atgccaccta cggcaagctg accctgaagc tgatctgcac caccggcaag ctgcccgtgc
      901 cctggcccac cctcgtgacc accctgggct acggcgtgca gtgcttcgcc cgctaccccg
      961 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     1021 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     1081 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     1141 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc accgccgaca
     1201 agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag gacggcggcg
     1261 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     1321 ccgacaacca ctacctgagc taccagtcca agctgagcaa agaccccaac gagaagcgcg
     1381 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     1441 tgtacaagta agtcgacggc gcgccgcggc cgcgaattcg atatcataat caacctctgg
     1501 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     1561 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     1621 tctcctcctt gtataaatcc tggttagttc ttgccacggc ggaactcatc gccgcctgcc
     1681 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gctcgagaga
     1741 tcttcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     1801 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     1861 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     1921 gaggattggg aagacaatag caggcatgag atctcacgtg cggaccgagc ggccgcagga
     1981 acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg
     2041 gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc
     2101 gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     2161 ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg
     2221 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     2281 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     2341 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     2401 cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     2461 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     2521 aaccctatct cgggctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     2581 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt
     2641 acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
     2701 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     2761 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     2821 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     2881 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     2941 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     3001 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     3061 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     3121 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     3181 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     3241 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     3301 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     3361 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     3421 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     3481 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     3541 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     3601 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     3661 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     3721 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     3781 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     3841 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     3901 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     3961 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     4021 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     4081 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     4141 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     4201 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     4261 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     4321 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     4381 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     4441 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     4501 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     4561 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     4621 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     4681 ttcctggcct tttgctggcc ttttgctcac atgt
//
