LOCUS       CN3591_pAAV-3xcore3_eHGT_512h-minBG-SYFP2-WPRE3-BGHpA        4789 bp    DNA     circular UNA 27-JUL-2021
DEFINITION  .
ACCESSION   urn.local...5t-dnv0pwk
VERSION     urn.local...5t-dnv0pwk
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     ##Author-START##
            Name :: Jonathan Ting
            ##Author-END##
            ##Comment-START##
            Comment :: rAAV-hPTenh(FAM84b)-minBG-SYFP2-WPRE3-BGHpA
                       clones 2,3,4 all good
                       clone 1 low yield
            ##Comment-END##
            ##Imported_From-START##
            Path     :: C:\VNTI Database
            Filename :: _exdata.ma4
            ##Imported_From-END##
FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /label="L-ITR"
     misc_feature    156..707
                     /Original_Bases="GGTACCCTAAATAAAGATGGCTTTTTAGTATTAAAAGTGGA
                     AGAAAATTACAGGTAATTATCTTTGACGGTAAAAACGCTGTAATCAGCGGGCTACATG
                     AAAAATTACTCTAATTATGGCTGCATTTAAGAGAATGGCTAAATAAAGATGGCTTTTT
                     AGTATTAAAAGTGGAAGAAAATTACAGGTAATTATCTTTGACGGTAAAAACGCTGTAA
                     TCAGCGGGCTACATGAAAAATTACTCTAATTATGGCTGCATTTAAGAGAATGGCTAAA
                     TAAAGATGGCTTTTTAGTATTAAAAGTGGAAGAAAATTACAGGTAATTATCTTTGACG
                     GTAAAAACGCTGTAATCAGCGGGCTACATGAAAAATTACTCTAATTATGGCTGCATTT
                     AAGAGAATG"
                     /label="GGTACCCTAAATAAAGATGGCTTTTTAGTATTAAAAGTGGAAGAAAATTA
                     CAGGTAATTATCTTTGACGGTAAAAACGCTGTAATCAGCGGGCTACATGAAAAATTAC
                     TCTAATTATGGCTGCATTTAAGAGAATGGCTAAATAAAGATGGCTTTTTAGTATTAAA
                     AGTGGAAGAAAATTACAGGTAATTATCTTTGACGGTAAAAACGCTGTAATCAGCGGGC
                     TACATGAAAAATTACTCTAATTATGGCTGCATTTAAGAGAATGGCTAAATAAAGATGG
                     CTTTTTAGTATTAAAAGTGGAAGAAAATTACAGGTAATTATCTTTGACGGTAAAAACG
                     CTGTAATCAGCGGGCTACATGAAAAATTACTCTAATTATGGCTGCATTTAAGAGAATG
                     "
                     /note="Geneious type: Editing History Replacement"
     misc_feature    156..707
                     /created_by="ximena.opitzaraya"
                     /label="3xcore3_eHGT_512h"
     misc_feature    714..766
                     /label="Beta Globin minimal promoter"
     misc_feature    723..728
                     /label="TATA box"
     misc_feature    complement(729..749)
                     /label="minBG-PCR-R"
     misc_feature    755
                     /label="transcriptional start"
     misc_feature    807..1526
                     /label="SYFP2"
     misc_feature    1561..1806
                     /label="WPRE3"
     misc_feature    1561..1720
                     /label="gamma"
     misc_feature    complement(1674..1693)
                     /label="WPRE3-R (JT)"
     misc_feature    1726..1806
                     /label="alpha"
     misc_feature    1820..2023
                     /label="BGH-pA"
     primer          complement(1934..1953)
                     /label="BGH-R"
     misc_feature    2052..2192
                     /label="R-ITR"
     rep_origin      2284..2590
                     /label="f1 Origin"
     misc_feature    3109..3966
                     /label="AmpR"
     rep_origin      4117..4784
                     /label="pUC ori"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtttgaa atcccatttc agcagaaaac
      181 tggctgggtt cctagccaaa aaagaacacc tgccagactg acttttccct aggtaatgta
      241 agaagcacag atgtcatcag tcactgttta cttaacaata tatttccact ggccacagtc
      301 tggccttgtt tcttccattt ctgcttccac ctctcatact tgaaatccca tttcagcaga
      361 aaactggctg ggttcctagc caaaaaagaa cacctgccag actgactttt ccctaggtaa
      421 tgtaagaagc acagatgtca tcagtcactg tttacttaac aatatatttc cactggccac
      481 agtctggcct tgtttcttcc atttctgctt ccacctctca tacttgaaat cccatttcag
      541 cagaaaactg gctgggttcc tagccaaaaa agaacacctg ccagactgac ttttccctag
      601 gtaatgtaag aagcacagat gtcatcagtc actgtttact taacaatata tttccactgg
      661 ccacagtctg gccttgtttc ttccatttct gcttccacct ctcatacgag ctcgggctgg
      721 gcataaaagt cagggcagag ccatctattg cttacatttg cttctgggat ccagatcttt
      781 cgaagctagc gctaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg
      841 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
      901 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagctgatc tgcaccaccg
      961 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gggctacggc gtgcagtgct
     1021 tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     1081 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     1141 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     1201 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     1261 atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcaagatc cgccacaaca
     1321 tcgaggacgg cggcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     1381 gccccgtgct gctgcccgac aaccactacc tgagctacca gtccaagctg agcaaagacc
     1441 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     1501 tcggcatgga cgagctgtac aagtaagtcg acggcgcgcc gcggccgcga attcgatatc
     1561 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     1621 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     1681 gtatggcttt cattttctcc tccttgtata aatcctggtt agttcttgcc acggcggaac
     1741 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     1801 ccgtggctcg agagatcttc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     1861 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     1921 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     1981 caggacagca agggggagga ttgggaagac aatagcaggc atgagatctc acgtgcggac
     2041 cgagcggccg caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc
     2101 gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc
     2161 agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt ttctccttac
     2221 gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc
     2281 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     2341 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     2401 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     2461 ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag
     2521 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     2581 actggaacaa cactcaaccc tatctcgggc tattcttttg atttataagg gattttgccg
     2641 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     2701 aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca
     2761 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     2821 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     2881 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
     2941 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     3001 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     3061 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     3121 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     3181 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     3241 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     3301 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     3361 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     3421 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     3481 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     3541 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     3601 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     3661 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     3721 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     3781 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     3841 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     3901 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     3961 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     4021 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     4081 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     4141 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     4201 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     4261 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     4321 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     4381 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     4441 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     4501 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     4561 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     4621 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     4681 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     4741 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgt
//
