LOCUS       CN3600_pAAV-3xcore3_eHGT_528h-minBG-SYFP2-WPRE3-BGHpA        4939 bp    DNA     circular UNA 27-JUL-2021
DEFINITION  .
ACCESSION   urn.local...7k-dnv38a1
VERSION     urn.local...7k-dnv38a1
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     ##Author-START##
            Name :: Jonathan Ting
            ##Author-END##
            ##Comment-START##
            Comment :: rAAV-hPTenh(FAM84b)-minBG-SYFP2-WPRE3-BGHpA
                       clones 2,3,4 all good
                       clone 1 low yield
            ##Comment-END##
            ##Imported_From-START##
            Path     :: C:\VNTI Database
            Filename :: _exdata.ma4
            ##Imported_From-END##
FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /label="L-ITR"
     misc_feature    156..856
                     /Original_Bases="GGTACCCTAAATAAAGATGGCTTTTTAGTATTAAAAGTGGA
                     AGAAAATTACAGGTAATTATCTTTGACGGTAAAAACGCTGTAATCAGCGGGCTACATG
                     AAAAATTACTCTAATTATGGCTGCATTTAAGAGAATGGCTAAATAAAGATGGCTTTTT
                     AGTATTAAAAGTGGAAGAAAATTACAGGTAATTATCTTTGACGGTAAAAACGCTGTAA
                     TCAGCGGGCTACATGAAAAATTACTCTAATTATGGCTGCATTTAAGAGAATGGCTAAA
                     TAAAGATGGCTTTTTAGTATTAAAAGTGGAAGAAAATTACAGGTAATTATCTTTGACG
                     GTAAAAACGCTGTAATCAGCGGGCTACATGAAAAATTACTCTAATTATGGCTGCATTT
                     AAGAGAAT"
                     /label="GGTACCCTAAATAAAGATGGCTTTTTAGTATTAAAAGTGGAAGAAAATTA
                     CAGGTAATTATCTTTGACGGTAAAAACGCTGTAATCAGCGGGCTACATGAAAAATTAC
                     TCTAATTATGGCTGCATTTAAGAGAATGGCTAAATAAAGATGGCTTTTTAGTATTAAA
                     AGTGGAAGAAAATTACAGGTAATTATCTTTGACGGTAAAAACGCTGTAATCAGCGGGC
                     TACATGAAAAATTACTCTAATTATGGCTGCATTTAAGAGAATGGCTAAATAAAGATGG
                     CTTTTTAGTATTAAAAGTGGAAGAAAATTACAGGTAATTATCTTTGACGGTAAAAACG
                     CTGTAATCAGCGGGCTACATGAAAAATTACTCTAATTATGGCTGCATTTAAGAGAAT"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    156..857
                     /created_by="ximena.opitzaraya"
                     /label="3xcore3_eHGT_528h"
     misc_feature    864..916
                     /label="Beta Globin minimal promoter"
     misc_feature    873..878
                     /label="TATA box"
     misc_feature    complement(879..899)
                     /label="minBG-PCR-R"
     misc_feature    905
                     /label="transcriptional start"
     misc_feature    957..1676
                     /label="SYFP2"
     misc_feature    1711..1956
                     /label="WPRE3"
     misc_feature    1711..1870
                     /label="gamma"
     misc_feature    complement(1824..1843)
                     /label="WPRE3-R (JT)"
     misc_feature    1876..1956
                     /label="alpha"
     misc_feature    1970..2173
                     /label="BGH-pA"
     primer          complement(2084..2103)
                     /label="BGH-R"
     misc_feature    2202..2342
                     /label="R-ITR"
     rep_origin      2434..2740
                     /label="f1 Origin"
     misc_feature    3259..4116
                     /label="AmpR"
     rep_origin      4267..4934
                     /label="pUC ori"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtataca ttgattttca ttctgatgta
      181 agccatttca cttttgattg ggacttcaca gaattctttt catagctggc ctctctcctc
      241 attctgatgg aaatctgcaa gctaaaggca gtcctgatga taggaagcaa aacagcaacc
      301 tcagaaggat ttcaaaatga ttcattttct ttacaaaaat atatatcatt agcctaacat
      361 aagtaagcgt tgaatattat gatcttgaga tacattgatt ttcattctga tgtaagccat
      421 ttcacttttg attgggactt cacagaattc ttttcatagc tggcctctct cctcattctg
      481 atggaaatct gcaagctaaa ggcagtcctg atgataggaa gcaaaacagc aacctcagaa
      541 ggatttcaaa atgattcatt ttctttacaa aaatatatat cattagccta acataagtaa
      601 gcgttgaata ttatgatctt gagatacatt gattttcatt ctgatgtaag ccatttcact
      661 tttgattggg acttcacaga attcttttca tagctggcct ctctcctcat tctgatggaa
      721 atctgcaagc taaaggcagt cctgatgata ggaagcaaaa cagcaacctc agaaggattt
      781 caaaatgatt cattttcttt acaaaaatat atatcattag cctaacataa gtaagcgttg
      841 aatattatga tcttgaggag ctcgggctgg gcataaaagt cagggcagag ccatctattg
      901 cttacatttg cttctgggat ccagatcttt cgaagctagc gctaccggtc gccaccatgg
      961 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     1021 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     1081 agctgaccct gaagctgatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     1141 tgaccaccct gggctacggc gtgcagtgct tcgcccgcta ccccgaccac atgaagcagc
     1201 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     1261 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     1321 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     1381 tggagtacaa ctacaacagc cacaacgtct atatcaccgc cgacaagcag aagaacggca
     1441 tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg cggcgtgcag ctcgccgacc
     1501 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     1561 tgagctacca gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     1621 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaagtcg
     1681 acggcgcgcc gcggccgcga attcgatatc ataatcaacc tctggattac aaaatttgtg
     1741 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     1801 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     1861 aatcctggtt agttcttgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     1921 caggggctcg gctgttgggc actgacaatt ccgtggctcg agagatcttc gactgtgcct
     1981 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     2041 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     2101 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     2161 aatagcaggc atgagatctc acgtgcggac cgagcggccg caggaacccc tagtgatgga
     2221 gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc
     2281 ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gctgcctgca
     2341 ggggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg
     2401 tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     2461 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     2521 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     2581 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat
     2641 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     2701 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcgggc
     2761 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     2821 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat tttatggtgc
     2881 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
     2941 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     3001 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
     3061 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     3121 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     3181 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     3241 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     3301 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     3361 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     3421 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     3481 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     3541 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     3601 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     3661 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     3721 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     3781 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     3841 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     3901 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     3961 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     4021 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     4081 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     4141 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     4201 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     4261 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     4321 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     4381 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     4441 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     4501 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     4561 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     4621 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     4681 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     4741 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     4801 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     4861 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     4921 tggccttttg ctcacatgt
//
