LOCUS       CN4186_pAAV-3xmPT(L5)corei-minBglobin-iCre(R297T)-BGHpA        4766 bp    DNA     circular     13-MAY-2022
DEFINITION  .
ACCESSION   urn.local...1ic-etaucea
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     ##Author-START##
            Name :: Jonathan Ting
            ##Author-END##
            ##Imported_From-START##
            Path     :: \\allen\programs\celltypes\workgroups\0285\CN-VectorNTI
            Filename :: CN3084 (JT).gb
            ##Imported_From-END##
FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /vntifkey=21
                     /label="L-ITR"
     misc_feature    156..625
                     /Original_Bases="ATAGGTACCGAGCTCTATGCACTCACAGTGGTTTGGCATGC
                     ATCTGGTGAATTTTTTTTAACGAAAAATTAGTGTTGGTTTCGATGTATGGTAGCATTC
                     TCCCTAACGTAATTTGAATAATTCAGCAAAGCCCCACTACCAGCTGTACTTCTGCAGC
                     CTCTTCCATTCTTTTCAGCATTATAATTTTGGTTAATTTTCAATTTTAGGTCCTACGT
                     CTCTGCAATTTGTGTATGAATAACAGAATAATTTCCCTCTTTTGTTTCGCCTTTCCTG
                     TTCCTGAATCTAAATAAAGATGGCTTTTTAGTATTAAAAGTGGAAGAAAATTACAGGT
                     AATTATCTTTGACGGTAAAAACGCTGTAATCAGCGGGCTACATGAAAAATTACTCTAA
                     TTATGGCTGCATTTAAGAGAATGGAAAAAAACCTTCTTGTGGATAAAAACCTTAAATT
                     GTCCCCAATGTCTGCTTCAAATTGGATGGCACTGCAGCTGGAGGCTTTGTTCAGAATT
                     GATCCTGGGGAGCTACGAACCCAAAGTTTCACAGTAG"
                     /label="ATAGGTACCGAGCTCTATGCACTCACAGTGGTTTGGCATGCATCTGGTGA
                     ATTTTTTTTAACGAAAAATTAGTGTTGGTTTCGATGTATGGTAGCATTCTCCCTAACG
                     TAATTTGAATAATTCAGCAAAGCCCCACTACCAGCTGTACTTCTGCAGCCTCTTCCAT
                     TCTTTTCAGCATTATAATTTTGGTTAATTTTCAATTTTAGGTCCTACGTCTCTGCAAT
                     TTGTGTATGAATAACAGAATAATTTCCCTCTTTTGTTTCGCCTTTCCTGTTCCTGAAT
                     CTAAATAAAGATGGCTTTTTAGTATTAAAAGTGGAAGAAAATTACAGGTAATTATCTT
                     TGACGGTAAAAACGCTGTAATCAGCGGGCTACATGAAAAATTACTCTAATTATGGCTG
                     CATTTAAGAGAATGGAAAAAAACCTTCTTGTGGATAAAAACCTTAAATTGTCCCCAAT
                     GTCTGCTTCAAATTGGATGGCACTGCAGCTGGAGGCTTTGTTCAGAATTGATCCTGGG
                     GAGCTACGAACCCAAAGTTTCACAGTAG"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    156..620
                     /label="3xmPT(L5)core"
     misc_feature    633..685
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey=21
                     /label="Beta\Globin\minimal\promoter"
     misc_feature    642..647
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey=21
                     /label="TATA\box"
     misc_feature    674
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey=21
                     /label="transcriptional\start"
     misc_feature    714..1769
                     /vntifkey=21
                     /label="iCre(297T)"
     misc_feature    714..740
                     /vntifkey=21
                     /label="nls"
     misc_feature    1626..1628
                     /vntifkey=21
                     /label="R297T"
     misc_feature    1689..1691
                     /vntifkey=21
                     /label="V318"
     misc_feature    1757
                     /vntifkey=21
                     /label="G-->A\silent\mutation\to\remove\XhoI\site"
     misc_feature    1803..2006
                     /vntifkey=21
                     /label="BGH-pA"
     primer          complement(1917..1936)
                     /vntifkey=27
                     /label="BGH-R"
     misc_feature    2029..2169
                     /vntifkey=21
                     /label="R-ITR"
     rep_origin      2261..2567
                     /vntifkey=33
                     /label="f1\Origin"
     misc_feature    3086..3943
                     /vntifkey=21
                     /label="AmpR"
     rep_origin      4094..4761
                     /vntifkey=33
                     /label="pUC\ori"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtttacc ctgttgaata tcactgactc
      181 actaacttgc attgccatgc taacttgctt tcagagagat ctcagaacac atcatcttct
      241 gctatttcaa tacatgcaca ttaatttcct atcaacgtgt gctgatcagg aactctgtaa
      301 tctggcaccg ttaccctgtt gaatatcact gactcactaa cttgcattgc catgctaact
      361 tgctttcaga gagatctcag aacacatcat cttctgctat ttcaatacat gcacattaat
      421 ttcctatcaa cgtgtgctga tcaggaactc tgtaatctgg caccgttacc ctgttgaata
      481 tcactgactc actaacttgc attgccatgc taacttgctt tcagagagat ctcagaacac
      541 atcatcttct gctatttcaa tacatgcaca ttaatttcct atcaacgtgt gctgatcagg
      601 aactctgtaa tctggcaccg cttaaggagc tcgggctggg cataaaagtc agggcagagc
      661 catctattgc ttacatttgc ttctgggatc cagatctttc caagctagcc accatggtgc
      721 ccaagaagaa gaggaaagtc tccaacctgc tgactgtgca ccaaaacctg cctgccctcc
      781 ctgtggatgc cacctctgat gaagtcagga agaacctgat ggacatgttc agggacaggc
      841 aggccttctc tgaacacacc tggaagatgc tcctgtctgt gtgcagatcc tgggctgcct
      901 ggtgcaagct gaacaacagg aaatggttcc ctgctgaacc tgaggatgtg agggactacc
      961 tcctgtacct gcaagccaga ggcctggctg tgaagaccat ccaacagcac ctgggccagc
     1021 tcaacatgct gcacaggaga tctggcctgc ctcgcccttc tgactccaat gctgtgtccc
     1081 tggtgatgag gagaatcaga aaggagaatg tggatgctgg ggagagagcc aagcaggccc
     1141 tggcctttga acgcactgac tttgaccaag tcagatccct gatggagaac tctgacagat
     1201 gccaggacat caggaacctg gccttcctgg gcattgccta caacaccctg ctgcgcattg
     1261 ccgaaattgc cagaatcaga gtgaaggaca tctcccgcac cgatggtggg agaatgctga
     1321 tccacattgg caggaccaag accctggtgt ccacagctgg tgtggagaag gccctgtccc
     1381 tgggggttac caagctggtg gagagatgga tctctgtgtc tggtgtggct gatgacccca
     1441 acaactacct gttctgccgg gtcagaaaga atggtgtggc tgccccttct gccacctccc
     1501 aactgtccac ccgggccctg gaagggatct ttgaggccac ccaccgcctg atctatggtg
     1561 ccaaggatga ctctgggcag agatacctgg cctggtctgg ccactctgcc agagtgggtg
     1621 ctgccaccga catggccagg gctggtgtgt ccatccctga aatcatgcag gctggtggct
     1681 ggaccaatgt gaacattgtg atgaactaca tcagaaacct ggactctgag actggggcca
     1741 tggtgaggct gctcgaagat ggggactgag gcgcgccgaa ttcaagcttc tcgagagatc
     1801 ttcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     1861 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     1921 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     1981 ggattgggaa gacaatagca ggcatgcacg tgcggaccga gcggccgcag gaacccctag
     2041 tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa
     2101 aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct
     2161 gcctgcaggg gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     2221 gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     2281 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     2341 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     2401 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt
     2461 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     2521 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     2581 ctcgggctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     2641 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt
     2701 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
     2761 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     2821 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     2881 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     2941 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     3001 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     3061 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     3121 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     3181 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     3241 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     3301 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     3361 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     3421 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     3481 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     3541 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     3601 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     3661 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     3721 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     3781 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     3841 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     3901 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     3961 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     4021 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     4081 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     4141 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     4201 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     4261 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     4321 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     4381 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     4441 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     4501 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     4561 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     4621 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     4681 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     4741 cttttgctgg ccttttgctc acatgt
//
