LOCUS       CN4286_pAAV-3xc3_eHGT_356h-minBglobin-SYFP2-WPRE3-BGHpA        4675 bp    DNA     circular UNA 25-AUG-2022
DEFINITION  .
ACCESSION   urn.local...l7-f85bpfm
VERSION     urn.local...l7-f85bpfm
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     ##Comment-START##
            Comment :: rAAV-ESYN-SYFP2-WPRE3-BGHpA
                       clone #8-1 correct , 8-2 backup
            ##Comment-END##
            ##Imported_From-START##
            Path     :: C:\VNTI Database
            Filename :: _exdata.ma4
            ##Imported_From-END##
FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /label="L-ITR"
     misc_feature    156..598
                     /Original_Bases="ATAGGTACCGAGCTCTATGCACTCACAGTGGTTTGGCATGC
                     ATCTGGTGAATTTTTTTTAACGAAAAATTAGTGTTGGTTTCGATGTATGGTAGCATTC
                     TCCCTAACGTAATTTGAATAATTCAGCAAAGCCCCACTACCAGCTGTACTTCTGCAGC
                     CTCTTCCATTCTTTTCAGCATTATAATTTTGGTTAATTTTCAATTTTAGGTCCTACGT
                     CTCTGCAATTTGTGTATGAATAACAGAATAATTTCCCTCTTTTGTTTCGCCTTTCCTG
                     TTCCTGAATCTAAATAAAGATGGCTTTTTAGTATTAAAAGTGGAAGAAAATTACAGGT
                     AATTATCTTTGACGGTAAAAACGCTGTAATCAGCGGGCTACATGAAAAATTACTCTAA
                     TTATGGCTGCATTTAAGAGAATGGAAAAAAACCTTCTTGTGGATAAAAACCTTAAATT
                     GTCCCCAATGTCTGCTTCAAATTGGATGGCACTGCAGCTGGAGGCTTTGTTCAGAATT
                     GATCCTGGGGAGCTACGAACCCAAAGTTTCACAGTAG"
                     /label="ATAGGTACCGAGCTCTATGCACTCACAGTGGTTTGGCATGCATCTGGTGA
                     ATTTTTTTTAACGAAAAATTAGTGTTGGTTTCGATGTATGGTAGCATTCTCCCTAACG
                     TAATTTGAATAATTCAGCAAAGCCCCACTACCAGCTGTACTTCTGCAGCCTCTTCCAT
                     TCTTTTCAGCATTATAATTTTGGTTAATTTTCAATTTTAGGTCCTACGTCTCTGCAAT
                     TTGTGTATGAATAACAGAATAATTTCCCTCTTTTGTTTCGCCTTTCCTGTTCCTGAAT
                     CTAAATAAAGATGGCTTTTTAGTATTAAAAGTGGAAGAAAATTACAGGTAATTATCTT
                     TGACGGTAAAAACGCTGTAATCAGCGGGCTACATGAAAAATTACTCTAATTATGGCTG
                     CATTTAAGAGAATGGAAAAAAACCTTCTTGTGGATAAAAACCTTAAATTGTCCCCAAT
                     GTCTGCTTCAAATTGGATGGCACTGCAGCTGGAGGCTTTGTTCAGAATTGATCCTGGG
                     GAGCTACGAACCCAAAGTTTCACAGTAG"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    156..599
                     /created_by="ximena.opitzaraya"
                     /label="3xc3_eHGT_356h"
     misc_feature    606..658
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Beta Globin minimal promoter"
     misc_feature    615..620
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="TATA box"
     misc_feature    647
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="transcriptional start"
     misc_feature    699..1418
                     /label="SYFP2"
     primer_bind     745..765
                     /label="EGFP-N-R"
     misc_feature    1453..1698
                     /label="WPRE3"
     misc_feature    1712..1915
                     /label="BGH-pA"
     primer          complement(1826..1845)
                     /label="BGH-R"
     misc_feature    1938..2078
                     /label="R-ITR"
     rep_origin      2170..2476
                     /label="f1 Origin"
     misc_feature    2995..3852
                     /label="AmpR"
     rep_origin      4003..4670
                     /label="pUC ori"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtggtgt gacagcctac tgcagctgtc
      181 cagccaatta agccctgtag gaagaatggc tgatgccgca gtctatgtga tgctgaactg
      241 cggtagccag gctgacctgg ccccaatccc gggctgtgac tgaccttcag atgcaggcag
      301 cagaggtgac agcctactgc agctgtccag ccaattaagc cctgtaggaa gaatggctga
      361 tgccgcagtc tatgtgatgc tgaactgcgg tagccaggct gacctggccc caatcccggg
      421 ctgtgactga ccttcagatg caggcagcag aggtgacagc ctactgcagc tgtccagcca
      481 attaagccct gtaggaagaa tggctgatgc cgcagtctat gtgatgctga actgcggtag
      541 ccaggctgac ctggccccaa tcccgggctg tgactgacct tcagatgcag gcagcagagg
      601 agctcgggct gggcataaaa gtcagggcag agccatctat tgcttacatt tgcttctggg
      661 atccagatct ttcgaagcta gcgctaccgg tcgccaccat ggtgagcaag ggcgaggagc
      721 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
      781 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagctga
      841 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgggctacg
      901 gcgtgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
      961 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     1021 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     1081 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     1141 gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaaga
     1201 tccgccacaa catcgaggac ggcggcgtgc agctcgccga ccactaccag cagaacaccc
     1261 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagctac cagtccaagc
     1321 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     1381 ccgggatcac tctcggcatg gacgagctgt acaagtaagt cgacggcgcg ccgcggccgc
     1441 gaattcgata tcataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     1501 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     1561 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttagttcttg
     1621 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     1681 gcactgacaa ttccgtggct cgagagatct tcgactgtgc cttctagttg ccagccatct
     1741 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     1801 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     1861 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgcacgt
     1921 gcggaccgag cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc
     1981 tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc
     2041 ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct
     2101 ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc
     2161 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     2221 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     2281 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     2341 cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc
     2401 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     2461 ttccaaactg gaacaacact caaccctatc tcgggctatt cttttgattt ataagggatt
     2521 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     2581 tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat
     2641 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     2701 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     2761 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
     2821 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     2881 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     2941 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     3001 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     3061 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     3121 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     3181 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     3241 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     3301 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     3361 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     3421 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     3481 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     3541 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     3601 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     3661 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     3721 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     3781 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     3841 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     3901 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     3961 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     4021 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     4081 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     4141 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     4201 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     4261 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     4321 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     4381 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     4441 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     4501 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     4561 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     4621 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgt
//
