LOCUS       CN4760_pAAV-MGT_E135-minBglobin-iCre-BGHpA        5946 bp    DNA     circular     18-JAN-2023
DEFINITION  .
ACCESSION   urn.local...d0-ft0u3j3
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     ##Author-START##
            Name :: Jonathan Ting
            ##Author-END##
            ##Imported_From-START##
            Path     :: \\allen\programs\celltypes\workgroups\0285\CN-VectorNTI
            Filename :: CN3084 (JT).gb
            ##Imported_From-END##
FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /vntifkey=21
                     /label="L-ITR"
     misc_feature    156..1803
                     /Original_Bases="ATAGGTACCGAGCTCTATGCACTCACAGTGGTTTGGCATGC
                     ATCTGGTGAATTTTTTTTAACGAAAAATTAGTGTTGGTTTCGATGTATGGTAGCATTC
                     TCCCTAACGTAATTTGAATAATTCAGCAAAGCCCCACTACCAGCTGTACTTCTGCAGC
                     CTCTTCCATTCTTTTCAGCATTATAATTTTGGTTAATTTTCAATTTTAGGTCCTACGT
                     CTCTGCAATTTGTGTATGAATAACAGAATAATTTCCCTCTTTTGTTTCGCCTTTCCTG
                     TTCCTGAATCTAAATAAAGATGGCTTTTTAGTATTAAAAGTGGAAGAAAATTACAGGT
                     AATTATCTTTGACGGTAAAAACGCTGTAATCAGCGGGCTACATGAAAAATTACTCTAA
                     TTATGGCTGCATTTAAGAGAATGGAAAAAAACCTTCTTGTGGATAAAAACCTTAAATT
                     GTCCCCAATGTCTGCTTCAAATTGGATGGCACTGCAGCTGGAGGCTTTGTTCAGAATT
                     GATCCTGGGGAGCTACGAACCCAAAGTTTCACAGTAG"
                     /label="ATAGGTACCGAGCTCTATGCACTCACAGTGGTTTGGCATGCATCTGGTGA
                     ATTTTTTTTAACGAAAAATTAGTGTTGGTTTCGATGTATGGTAGCATTCTCCCTAACG
                     TAATTTGAATAATTCAGCAAAGCCCCACTACCAGCTGTACTTCTGCAGCCTCTTCCAT
                     TCTTTTCAGCATTATAATTTTGGTTAATTTTCAATTTTAGGTCCTACGTCTCTGCAAT
                     TTGTGTATGAATAACAGAATAATTTCCCTCTTTTGTTTCGCCTTTCCTGTTCCTGAAT
                     CTAAATAAAGATGGCTTTTTAGTATTAAAAGTGGAAGAAAATTACAGGTAATTATCTT
                     TGACGGTAAAAACGCTGTAATCAGCGGGCTACATGAAAAATTACTCTAATTATGGCTG
                     CATTTAAGAGAATGGAAAAAAACCTTCTTGTGGATAAAAACCTTAAATTGTCCCCAAT
                     GTCTGCTTCAAATTGGATGGCACTGCAGCTGGAGGCTTTGTTCAGAATTGATCCTGGG
                     GAGCTACGAACCCAAAGTTTCACAGTAG"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    <156..172
                     /note="/Source=referenceToDocument:
                     urn:local:.:45g-dob6a3e"
                     /label="AiP1218 - pAAV-MGT_E57-minBGprom-FlpO-WPRE-hGHp"
                     /note="Geneious type: Concatenated sequence"
     enhancer        179..1796
                     /note="/created_by=francoise.haeseleer"
                     /modified_by="jacqueline.bendrick"
                     /label="MGT_E135"
     primer_bind     complement(1780..1822)
                     /Sequence="TATGCCCAGCCCGAGCTCCGCCGGCGAAGGTCTGCCGTGCCAC"
                     /%GC="72.1"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="MG_3436"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:francoise.haeseleer:b5-dqw9roo"">MG_3436<
                     /a>"
                     /label="MG_3436"
     misc_feature    1803..1809
                     /note="/Source=referenceToDocument:
                     urn:local:.:45g-dob6a3e"
                     /label="AiP1218 - pAAV-MGT_E57-minBGprom-FlpO-WPRE-hGHp"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    complement(1806..1828)
                     /note="/Source=referenceToDocument:
                     urn:local:.:45h-dob6a3e"
                     /label="& Trimmed m453-RMSYFP2seqR.ab1 (concatenated se"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    join(1806..1809,1810..1828)
                     /label="Ligation"
     promoter        1811..1863
                     /locus_tag="Beta\Globin\minimal\promoter"
                     /note="/ApEinfo_fwdcolor=#7eff74"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1}
                     {} 0}<br>width 5 offset 0"
                     /note="/ApEinfo_label=Beta\Globin\minimal\promoter"
                     /note="/ApEinfo_revcolor=#7eff74"
                     /note="/vntifkey=21"
                     /modified_by="jacqueline.bendrick"
                     /label="beta-globin minimal promoter"
     misc_feature    1820..1825
                     /locus_tag="TATA\box"
                     /note="/ApEinfo_fwdcolor=#7eff74"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1}
                     {} 0}<br>width 5 offset 0"
                     /note="/ApEinfo_label=TATA\box"
                     /note="/ApEinfo_revcolor=#7eff74"
                     /note="/vntifkey=21"
                     /label="TATA box"
     misc_feature    1852
                     /locus_tag="transcriptional\start"
                     /note="/ApEinfo_fwdcolor=#7eff74"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1}
                     {} 0}<br>width 5 offset 0"
                     /note="/ApEinfo_label=transcriptional\start"
                     /note="/ApEinfo_revcolor=#7eff74"
                     /note="/vntifkey=21"
                     /label="transcriptional start"
     misc_feature    1879
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    1888..2946
                     /vntifkey=21
                     /label="iCre"
     misc_feature    1888..2946
                     /Original_Bases="CACCATGGTGCCCAAGAAGAAGAGGAAAGTCTCCAACCTGC
                     TGACTGTGCACCAAAACCTGCCTGCCCTCCCTGTGGATGCCACCTCTGATGAAGTCAG
                     GAAGAACCTGATGGACATGTTCAGGGACAGGCAGGCCTTCTCTGAACACACCTGGAAG
                     ATGCTCCTGTCTGTGTGCAGATCCTGGGCTGCCTGGTGCAAGCTGAACAACAGGAAAT
                     GGTTCCCTGCTGAACCTGAGGATGTGAGGGACTACCTCCTGTACCTGCAAGCCAGAGG
                     CCTGGCTGTGAAGACCATCCAACAGCACCTGGGCCAGCTCAACATGCTGCACAGGAGA
                     TCTGGCCTGCCTCGCCCTTCTGACTCCAATGCTGTGTCCCTGGTGATGAGGAGAATCA
                     GAAAGGAGAATGTGGATGCTGGGGAGAGAGCCAAGCAGGCCCTGGCCTTTGAACGCAC
                     TGACTTTGACCAAGTCAGATCCCTGATGGAGAACTCTGACAGATGCCAGGACATCAGG
                     AACCTGGCCTTCCTGGGCATTGCCTACAACACCCTGCTGCGCATTGCCGAAATTGCCA
                     GAATCAGAGTGAAGGACATCTCCCGCACCGATGGTGGGAGAATGCTGATCCACATTGG
                     CAGGACCAAGACCCTGGTGTCCACAGCTGGTGTGGAGAAGGCCCTGTCCCTGGGGGTT
                     ACCAAGCTGGTGGAGAGATGGATCTCTGTGTCTGGTGTGGCTGATGACCCCAACAACT
                     ACCTGTTCTGCCGGGTCAGAAAGAATGGTGTGGCTGCCCCTTCTGCCACCTCCCAACT
                     GTCCACCCGGGCCCTGGAAGGGATCTTTGAGGCCACCCACCGCCTGATCTATGGTGCC
                     AAGGATGACTCTGGGCAGAGATACCTGGCCTGGTCTGGCCACTCTGCCAGAGTGGGTG
                     CTGCCACCGACATGGCCAGGGCTGGTGTGTCCATCCCTGAAATCATGCAGGCTGGTGG
                     CTGGACCAATGTGAACATTGTGATGAACTACATCAGAAACCTGGACTCTGAGACTGGG
                     GCCATGGTGAGGCTGCTCGAAGATGGGGAC"
                     /label="CACCATGGTGCCCAAGAAGAAGAGGAAAGTCTCCAACCTGCTGACTGTGC
                     ACCAAAACCTGCCTGCCCTCCCTGTGGATGCCACCTCTGATGAAGTCAGGAAGAACCT
                     GATGGACATGTTCAGGGACAGGCAGGCCTTCTCTGAACACACCTGGAAGATGCTCCTG
                     TCTGTGTGCAGATCCTGGGCTGCCTGGTGCAAGCTGAACAACAGGAAATGGTTCCCTG
                     CTGAACCTGAGGATGTGAGGGACTACCTCCTGTACCTGCAAGCCAGAGGCCTGGCTGT
                     GAAGACCATCCAACAGCACCTGGGCCAGCTCAACATGCTGCACAGGAGATCTGGCCTG
                     CCTCGCCCTTCTGACTCCAATGCTGTGTCCCTGGTGATGAGGAGAATCAGAAAGGAGA
                     ATGTGGATGCTGGGGAGAGAGCCAAGCAGGCCCTGGCCTTTGAACGCACTGACTTTGA
                     CCAAGTCAGATCCCTGATGGAGAACTCTGACAGATGCCAGGACATCAGGAACCTGGCC
                     TTCCTGGGCATTGCCTACAACACCCTGCTGCGCATTGCCGAAATTGCCAGAATCAGAG
                     TGAAGGACATCTCCCGCACCGATGGTGGGAGAATGCTGATCCACATTGGCAGGACCAA
                     GACCCTGGTGTCCACAGCTGGTGTGGAGAAGGCCCTGTCCCTGGGGGTTACCAAGCTG
                     GTGGAGAGATGGATCTCTGTGTCTGGTGTGGCTGATGACCCCAACAACTACCTGTTCT
                     GCCGGGTCAGAAAGAATGGTGTGGCTGCCCCTTCTGCCACCTCCCAACTGTCCACCCG
                     GGCCCTGGAAGGGATCTTTGAGGCCACCCACCGCCTGATCTATGGTGCCAAGGATGAC
                     TCTGGGCAGAGATACCTGGCCTGGTCTGGCCACTCTGCCAGAGTGGGTGCTGCCACCG
                     ACATGGCCAGGGCTGGTGTGTCCATCCCTGAAATCATGCAGGCTGGTGGCTGGACCAA
                     TGTGAACATTGTGATGAACTACATCAGAAACCTGGACTCTGAGACTGGGGCCATGGTG
                     AGGCTGCTCGAAGATGGGGAC"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    complement(1992..2012)
                     /vntifkey=21
                     /label="iCre-seq-R\(JT)"
     misc_feature    2547
                     /vntifkey=21
                     /label="G-A\mutation?"
     misc_feature    2983..3186
                     /vntifkey=21
                     /label="BGH-pA"
     primer          complement(3097..3116)
                     /vntifkey=27
                     /label="BGH-R"
     misc_feature    3209..3349
                     /vntifkey=21
                     /label="R-ITR"
     rep_origin      3441..3747
                     /vntifkey=33
                     /label="f1\Origin"
     misc_feature    4266..5123
                     /vntifkey=21
                     /label="AmpR"
     rep_origin      5274..5941
                     /vntifkey=33
                     /label="pUC\ori"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgttgtaa gagtaataat cgccggcgtc
      181 gggaacatgg gggtcaagtg agagagccag gtgtctccag cactcagcat gatatctgac
      241 acaaatcaga cccctcactt ttaacactgt accctaagac ttggggcaca ggccagggac
      301 tttccgttct aggctctctg gctcctgctt cttggattct gatctccggt cttatcgact
      361 tcatttccaa attagaggtt ttttggaacc caggggctac caggccaacc tggaagtccg
      421 actgcacatg ctgtatccgt gacagagtta cagagaacag ggactgccct gagtgggaca
      481 ggccgaagag ggactgccag agaggtacag accccacccc cacctttgta agcctgattg
      541 cctacccagg ccaccacaac tccatccagc tctgtccccg cctttgttcc tgagtctcca
      601 aggccccctt tcctgccaaa caaagccccc agccacccga ctgccgccca acaggcacct
      661 ggggcctctg ggcctgggaa tcaaagcctg ttttgcaaag gaggcccagt gtttggagaa
      721 cacccatccc ctgctgcccg gtccttcaaa gaaccggcat tgttctgcgc tgagaaggcg
      781 cctcttgacg cgctgggcag ggtcggcttg acgctggtgg acctctgatc tcaggccagg
      841 cccccagcca cttgggggga gggtggactt aggggccaaa gagggagcaa cctgtccttg
      901 gggcactgtg gtccttcctc attctctgcc cacccgagtt cccatcaccg agttcattga
      961 gacccaggct cttctacctg gtgatctgga caaggcaggc ctcaggaggc accaggacaa
     1021 gagatgagat agcgggcagg attttagtga ccataacagg tacgtcagag actgcaaacg
     1081 tattaggcct cagtggcatg taactgtgat tctagtactt gggatgctga ggcaggagaa
     1141 tcatgagttt gaagttaacc ggatcttcaa ggcaagaccc tgtctctaaa tcatagatca
     1201 caggggagtg tgtgtttgtg tgtgtgtgtg tgcgtgcgtg cgcgcatgcg cgtgtacacg
     1261 tgcgtgtgca tatgtatgca tgttctcatt ttacagctca cagaggctag agaggaaact
     1321 gggcccagag cccggttcaa gctgtgaact gacaggcccc gttggctctt ttatttgttg
     1381 aagaaatatt tcccaattac tttcttgtcc attgtggatt ccactgcaag ctaacattag
     1441 taaaggcatt taaataaaag tctgcccaga cacaaactaa ggccacagaa ctggctgtgg
     1501 tgcccaggcg ggtcgtgagg agcccaaata acactcaaaa gccaccttta tttgtccttc
     1561 ccctctgcca gtcacagcat catgagcttc tggggtggtg tgtgtgtggg gggggtgtta
     1621 gtctcactcc tgtggcactg cagaagtctc tcaaggcaca gagagagggc aagcaatgtg
     1681 ttcaaagtca cccagaaact gaggttagat tggagcggag agcctcatct caggagctca
     1741 ttctgtccgg tcctcccctc agatcacact tctgatcaag tggcacggca gaccttcgcc
     1801 ggcggagctc gggctgggca taaaagtcag ggcagagcca tctattgctt acatttgctt
     1861 ctgggatcca gatctttcga agctagcacc atggtgccca agaagaagag gaaagtctcc
     1921 aacctgctga ctgtgcacca aaacctgcct gccctccctg tggatgccac ctctgatgaa
     1981 gtcaggaaga acctgatgga catgttcagg gacaggcagg ccttctctga acacacctgg
     2041 aagatgctcc tgtctgtgtg cagatcctgg gctgcctggt gcaagctgaa caacaggaaa
     2101 tggttccctg ctgaacctga ggatgtgagg gactacctcc tgtacctgca agccagaggc
     2161 ctggctgtga agaccatcca acagcacctg ggccagctca acatgctgca caggagatct
     2221 ggcctgcctc gcccttctga ctccaatgct gtgtccctgg tgatgaggag aatcagaaag
     2281 gagaatgtgg atgctgggga gagagccaag caggccctgg cctttgaacg cactgacttt
     2341 gaccaagtca gatccctgat ggagaactct gacagatgcc aggacatcag gaacctggcc
     2401 ttcctgggca ttgcctacaa caccctgctg cgcattgccg aaattgccag aatcagagtg
     2461 aaggacatct cccgcaccga tggtgggaga atgctgatcc acattggcag gaccaagacc
     2521 ctggtgtcca cagctggtgt ggagaaggcc ctgtccctgg gggttaccaa gctggtggag
     2581 agatggatct ctgtgtctgg tgtggctgat gaccccaaca actacctgtt ctgccgggtc
     2641 agaaagaatg gtgtggctgc cccttctgcc acctcccaac tgtccacccg ggccctggaa
     2701 gggatctttg aggccaccca ccgcctgatc tatggtgcca aggatgactc tgggcagaga
     2761 tacctggcct ggtctggcca ctctgccaga gtgggtgctg ccagggacat ggccagggct
     2821 ggtgtgtcca tccctgaaat catgcaggct ggtggctgga ccaatgtgaa cattgtgatg
     2881 aactacatca gaaacctgga ctctgagact ggggccatgg tgaggctgct cgaggatggg
     2941 gactaatgag gcgcgccgaa ttcaagcttc tcgagagatc ttcgactgtg ccttctagtt
     3001 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     3061 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     3121 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     3181 ggcatgcacg tgcggaccga gcggccgcag gaacccctag tgatggagtt ggccactccc
     3241 tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc
     3301 tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg
     3361 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat
     3421 agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     3481 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     3541 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     3601 ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg
     3661 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     3721 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcgggctat tcttttgatt
     3781 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     3841 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa
     3901 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     3961 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     4021 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     4081 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     4141 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     4201 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     4261 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     4321 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     4381 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     4441 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     4501 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     4561 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     4621 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     4681 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     4741 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     4801 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     4861 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     4921 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     4981 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     5041 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     5101 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     5161 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     5221 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     5281 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     5341 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     5401 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     5461 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     5521 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     5581 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     5641 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     5701 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     5761 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     5821 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     5881 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     5941 acatgt
//
