LOCUS       CN5080_pAAV-3xc2_eHGT_724m-minBglobin-SYFP2-WPRE3-BGHpA        4831 bp    DNA     circular UNA 14-AUG-2023
DEFINITION  .
ACCESSION   urn.local...eg-gh1m4gx
VERSION     urn.local...eg-gh1m4gx
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     ##Comment-START##
            Comment :: rAAV-ESYN-SYFP2-WPRE3-BGHpA
                       clone #8-1 correct , 8-2 backup
            ##Comment-END##
            ##Imported_From-START##
            Path     :: C:\VNTI Database
            Filename :: _exdata.ma4
            ##Imported_From-END##
FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /label="L-ITR"
     misc_feature    156..755
                     /Original_Bases="ATAGGTACCGAGCTCTATGCACTCACAGTGGTTTGGCATGC
                     ATCTGGTGAATTTTTTTTAACGAAAAATTAGTGTTGGTTTCGATGTATGGTAGCATTC
                     TCCCTAACGTAATTTGAATAATTCAGCAAAGCCCCACTACCAGCTGTACTTCTGCAGC
                     CTCTTCCATTCTTTTCAGCATTATAATTTTGGTTAATTTTCAATTTTAGGTCCTACGT
                     CTCTGCAATTTGTGTATGAATAACAGAATAATTTCCCTCTTTTGTTTCGCCTTTCCTG
                     TTCCTGAATCTAAATAAAGATGGCTTTTTAGTATTAAAAGTGGAAGAAAATTACAGGT
                     AATTATCTTTGACGGTAAAAACGCTGTAATCAGCGGGCTACATGAAAAATTACTCTAA
                     TTATGGCTGCATTTAAGAGAATGGAAAAAAACCTTCTTGTGGATAAAAACCTTAAATT
                     GTCCCCAATGTCTGCTTCAAATTGGATGGCACTGCAGCTGGAGGCTTTGTTCAGAATT
                     GATCCTGGGGAGCTACGAACCCAAAGTTTCACAGTAGG"
                     /label="ATAGGTACCGAGCTCTATGCACTCACAGTGGTTTGGCATGCATCTGGTGA
                     ATTTTTTTTAACGAAAAATTAGTGTTGGTTTCGATGTATGGTAGCATTCTCCCTAACG
                     TAATTTGAATAATTCAGCAAAGCCCCACTACCAGCTGTACTTCTGCAGCCTCTTCCAT
                     TCTTTTCAGCATTATAATTTTGGTTAATTTTCAATTTTAGGTCCTACGTCTCTGCAAT
                     TTGTGTATGAATAACAGAATAATTTCCCTCTTTTGTTTCGCCTTTCCTGTTCCTGAAT
                     CTAAATAAAGATGGCTTTTTAGTATTAAAAGTGGAAGAAAATTACAGGTAATTATCTT
                     TGACGGTAAAAACGCTGTAATCAGCGGGCTACATGAAAAATTACTCTAATTATGGCTG
                     CATTTAAGAGAATGGAAAAAAACCTTCTTGTGGATAAAAACCTTAAATTGTCCCCAAT
                     GTCTGCTTCAAATTGGATGGCACTGCAGCTGGAGGCTTTGTTCAGAATTGATCCTGGG
                     GAGCTACGAACCCAAAGTTTCACAGTAGG"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    156..755
                     /created_by="ximena.opitzaraya"
                     /label="3xc2_eHGT_724m"
     misc_feature    762..814
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Beta Globin minimal promoter"
     misc_feature    771..776
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="TATA box"
     misc_feature    803
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="transcriptional start"
     misc_feature    855..1574
                     /label="SYFP2"
     primer_bind     901..921
                     /label="EGFP-N-R"
     misc_feature    1609..1854
                     /label="WPRE3"
     misc_feature    1868..2071
                     /label="BGH-pA"
     primer          complement(1982..2001)
                     /label="BGH-R"
     misc_feature    2094..2234
                     /label="R-ITR"
     rep_origin      2326..2632
                     /label="f1 Origin"
     misc_feature    3151..4008
                     /label="AmpR"
     rep_origin      4159..4826
                     /label="pUC ori"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtttgga agctgtgctg gcaaattgtc
      181 aatggaacac atcaggatcc cggagccttc aacactaaga gaggcaacat cctttattca
      241 aatgcacaaa atatgacttg aaatttttcc cagactctac tccattccaa catttgtatc
      301 actgctatta atatatttat agtcttgaga aaaaggcata acactataaa caagattgga
      361 agctgtgctg gcaaattgtc aatggaacac atcaggatcc cggagccttc aacactaaga
      421 gaggcaacat cctttattca aatgcacaaa atatgacttg aaatttttcc cagactctac
      481 tccattccaa catttgtatc actgctatta atatatttat agtcttgaga aaaaggcata
      541 acactataaa caagattgga agctgtgctg gcaaattgtc aatggaacac atcaggatcc
      601 cggagccttc aacactaaga gaggcaacat cctttattca aatgcacaaa atatgacttg
      661 aaatttttcc cagactctac tccattccaa catttgtatc actgctatta atatatttat
      721 agtcttgaga aaaaggcata acactataaa caagagagct cgggctgggc ataaaagtca
      781 gggcagagcc atctattgct tacatttgct tctgggatcc agatctttcg aagctagcgc
      841 taccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
      901 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
      961 gcgatgccac ctacggcaag ctgaccctga agctgatctg caccaccggc aagctgcccg
     1021 tgccctggcc caccctcgtg accaccctgg gctacggcgt gcagtgcttc gcccgctacc
     1081 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     1141 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     1201 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     1261 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcaccgccg
     1321 acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc gaggacggcg
     1381 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     1441 tgcccgacaa ccactacctg agctaccagt ccaagctgag caaagacccc aacgagaagc
     1501 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     1561 agctgtacaa gtaagtcgac ggcgcgccgc ggccgcgaat tcgatatcat aatcaacctc
     1621 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     1681 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     1741 ttttctcctc cttgtataaa tcctggttag ttcttgccac ggcggaactc atcgccgcct
     1801 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggctcgag
     1861 agatcttcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     1921 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     1981 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     2041 ggggaggatt gggaagacaa tagcaggcat gcacgtgcgg accgagcggc cgcaggaacc
     2101 cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg
     2161 accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg
     2221 cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     2281 acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg
     2341 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     2401 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     2461 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     2521 gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     2581 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     2641 cctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     2701 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca
     2761 attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     2821 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     2881 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     2941 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     3001 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     3061 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     3121 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     3181 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     3241 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     3301 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     3361 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     3421 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     3481 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     3541 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     3601 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     3661 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     3721 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     3781 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     3841 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     3901 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     3961 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     4021 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     4081 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     4141 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     4201 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     4261 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     4321 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     4381 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     4441 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     4501 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     4561 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     4621 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     4681 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     4741 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     4801 ctggcctttt gctggccttt tgctcacatg t
//
