LOCUS       CN5290_pAAV-eHGT_456m-minBG-iCre(R297T)-BGHpA        5090 bp    DNA     linear       07-NOV-2023
DEFINITION  .
ACCESSION   urn.local...mnu-gxrigxp
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     rAAV-hI56i-minBG-IRES2-iCre(V318K)-BGHpA                           
            clone #1,2 correct                                                 
                                                                               
                                                                               
                                                                               
                                                                               
                                                                               
                                                                               
            This file is created by Vector NTI                                 
            http://www.invitrogen.com/                                         
            ORIGDB|GenBank                                                     
            VNTDATE|-13763174|                                                 
            VNTDBDATE|-13763174|                                               
            LSOWNER|                                                           
            VNTNAME|CN3084 pAAV-hI56i-minBglobin-iCre(R297T)-BGHpA (JT)|       
            VNTAUTHORNAME|Jonathan Ting|                                       
            VNTOAUTHORNAME|Feng Zhang|.
FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /vntifkey=21
                     /label="L-ITR"
     misc_feature    complement(<156..>950)
                     /Source="referenceToDocument: urn:local:.:mnv-gxrigxp"
                     /label="456m-RMSYFP2seqR.ab1 (concatenated sequence 2)
                     (reversed)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    156..950
                     /Color="#ffff55"
                     /created_by="refugio.martinez"
                     /label="eHGT_456m"
     misc_feature    156..950
                     /Original_Bases="ATAGGTACCGAGCTCTATGCACTCACAGTGGTTTGGCATGC
                     ATCTGGTGAATTTTTTTTAACGAAAAATTAGTGTTGGTTTCGATGTATGGTAGCATTC
                     TCCCTAACGTAATTTGAATAATTCAGCAAAGCCCCACTACCAGCTGTACTTCTGCAGC
                     CTCTTCCATTCTTTTCAGCATTATAATTTTGGTTAATTTTCAATTTTAGGTCCTACGT
                     CTCTGCAATTTGTGTATGAATAACAGAATAATTTCCCTCTTTTGTTTCGCCTTTCCTG
                     TTCCTGAATCTAAATAAAGATGGCTTTTTAGTATTAAAAGTGGAAGAAAATTACAGGT
                     AATTATCTTTGACGGTAAAAACGCTGTAATCAGCGGGCTACATGAAAAATTACTCTAA
                     TTATGGCTGCATTTAAGAGAATGGAAAAAAACCTTCTTGTGGATAAAAACCTTAAATT
                     GTCCCCAATGTCTGCTTCAAATTGGATGGCACTGCAGCTGGAGGCTTTGTTCAGAATT
                     GATCCTGGGGAGCTACGAACCCAAAGTTTCACAGTAGG"
                     /label="ATAGGTACCGAGCTCTATGCACTCACAGTGGTTTGGCATGCATCTGGTGA
                     ATTTTTTTTAACGAAAAATTAGTGTTGGTTTCGATGTATGGTAGCATTCTCCCTAACG
                     TAATTTGAATAATTCAGCAAAGCCCCACTACCAGCTGTACTTCTGCAGCCTCTTCCAT
                     TCTTTTCAGCATTATAATTTTGGTTAATTTTCAATTTTAGGTCCTACGTCTCTGCAAT
                     TTGTGTATGAATAACAGAATAATTTCCCTCTTTTGTTTCGCCTTTCCTGTTCCTGAAT
                     CTAAATAAAGATGGCTTTTTAGTATTAAAAGTGGAAGAAAATTACAGGTAATTATCTT
                     TGACGGTAAAAACGCTGTAATCAGCGGGCTACATGAAAAATTACTCTAATTATGGCTG
                     CATTTAAGAGAATGGAAAAAAACCTTCTTGTGGATAAAAACCTTAAATTGTCCCCAAT
                     GTCTGCTTCAAATTGGATGGCACTGCAGCTGGAGGCTTTGTTCAGAATTGATCCTGGG
                     GAGCTACGAACCCAAAGTTTCACAGTAGG"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    957..1009
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey=21
                     /label="Beta\Globin\minimal\promoter"
     misc_feature    966..971
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey=21
                     /label="TATA\box"
     misc_feature    998
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey=21
                     /label="transcriptional\start"
     misc_feature    1025
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    1038..2093
                     /vntifkey=21
                     /label="iCre(297T)"
     misc_feature    1038..1064
                     /vntifkey=21
                     /label="nls"
     misc_feature    1950..1952
                     /vntifkey=21
                     /label="R297T"
     misc_feature    2013..2015
                     /vntifkey=21
                     /label="V318"
     misc_feature    2081
                     /vntifkey=21
                     /label="G-->A\silent\mutation\to\remove\XhoI\site"
     misc_feature    2127..2330
                     /vntifkey=21
                     /label="BGH-pA"
     primer          complement(2241..2260)
                     /vntifkey=27
                     /label="BGH-R"
     misc_feature    2353..2493
                     /vntifkey=21
                     /label="R-ITR"
     rep_origin      2585..2891
                     /vntifkey=33
                     /label="f1\Origin"
     misc_feature    3410..4267
                     /vntifkey=21
                     /label="AmpR"
     rep_origin      4418..5085
                     /vntifkey=33
                     /label="pUC\ori"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgttggtt agcactattt tgaatggaca
      181 cttctataaa atttgtttta aaaacaatat ggaagagtca taagatgtct gggcacagtg
      241 cccccagtca actcatcaat gggattctgg aatccaaagg ttatatggtg taaaaacaca
      301 gagagcaaaa tgcacaagga tcttcataga accataattt ttaaaaagca aacacttccc
      361 ccaaaggagc atgtaagaca acttgaaggt attaaaatgg gattgtggtt ttattagtta
      421 gagaacatca tgcaaattat attatctagg cattataatg caaactttaa aaggaaatgc
      481 tcattatgta atgttaagtg aaaaaaatca ggccataaaa tgaaatgtgc attatgcatg
      541 catatgatga aaagacaaga aatagataag aatatgcaga aatacaaata gttggctgtg
      601 gtgtgggctt ctggcttgtt ttctgtttta aagccattac atcatctttc caatgacaaa
      661 ctacatttta agttattgaa aacaaaatct cgttagcctt gtccttgcat aaattcccag
      721 agaacacatt attcttgttc ccttctacct ctgctacttg tctttgattt ggacctgctc
      781 cacctcctct tacttttcag tcctgaacta gatggaaaaa agaaatagac cctcaggaaa
      841 caaggaagca aaaccaccca actgtgtgga agccgtgcat tcaagctgca aacgagtgga
      901 gtggcttcaa taccttaact tatccccatt gttgcaaagg gagacccaga gagctcgggc
      961 tgggcataaa agtcagggca gagccatcta ttgcttacat ttgcttctgg gatccagatc
     1021 tttcgaagct agccaccatg gtgcccaaga agaagaggaa agtctccaac ctgctgactg
     1081 tgcaccaaaa cctgcctgcc ctccctgtgg atgccacctc tgatgaagtc aggaagaacc
     1141 tgatggacat gttcagggac aggcaggcct tctctgaaca cacctggaag atgctcctgt
     1201 ctgtgtgcag atcctgggct gcctggtgca agctgaacaa caggaaatgg ttccctgctg
     1261 aacctgagga tgtgagggac tacctcctgt acctgcaagc cagaggcctg gctgtgaaga
     1321 ccatccaaca gcacctgggc cagctcaaca tgctgcacag gagatctggc ctgcctcgcc
     1381 cttctgactc caatgctgtg tccctggtga tgaggagaat cagaaaggag aatgtggatg
     1441 ctggggagag agccaagcag gccctggcct ttgaacgcac tgactttgac caagtcagat
     1501 ccctgatgga gaactctgac agatgccagg acatcaggaa cctggccttc ctgggcattg
     1561 cctacaacac cctgctgcgc attgccgaaa ttgccagaat cagagtgaag gacatctccc
     1621 gcaccgatgg tgggagaatg ctgatccaca ttggcaggac caagaccctg gtgtccacag
     1681 ctggtgtgga gaaggccctg tccctggggg ttaccaagct ggtggagaga tggatctctg
     1741 tgtctggtgt ggctgatgac cccaacaact acctgttctg ccgggtcaga aagaatggtg
     1801 tggctgcccc ttctgccacc tcccaactgt ccacccgggc cctggaaggg atctttgagg
     1861 ccacccaccg cctgatctat ggtgccaagg atgactctgg gcagagatac ctggcctggt
     1921 ctggccactc tgccagagtg ggtgctgcca ccgacatggc cagggctggt gtgtccatcc
     1981 ctgaaatcat gcaggctggt ggctggacca atgtgaacat tgtgatgaac tacatcagaa
     2041 acctggactc tgagactggg gccatggtga ggctgctcga agatggggac tgaggcgcgc
     2101 cgaattcaag cttctcgaga gatcttcgac tgtgccttct agttgccagc catctgttgt
     2161 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     2221 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     2281 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg cacgtgcgga
     2341 ccgagcggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct
     2401 cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct
     2461 cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat tttctcctta
     2521 cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag
     2581 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     2641 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     2701 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     2761 cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata
     2821 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     2881 aactggaaca acactcaacc ctatctcggg ctattctttt gatttataag ggattttgcc
     2941 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     3001 caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc
     3061 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     3121 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     3181 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
     3241 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     3301 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     3361 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     3421 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     3481 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     3541 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     3601 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     3661 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     3721 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     3781 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     3841 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     3901 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     3961 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     4021 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     4081 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     4141 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     4201 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     4261 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     4321 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     4381 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     4441 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     4501 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     4561 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     4621 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     4681 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     4741 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     4801 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     4861 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     4921 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     4981 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     5041 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
//
