LOCUS       CN4211_pAAV-3xhI56i(core)-minBG-mScarlet-WPRE3-BGHpA        4604 bp    DNA     circular UNA 22-JUL-2022
DEFINITION  .
ACCESSION   urn.local...h8-eu184d9
VERSION     urn.local...h8-eu184d9
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     ##Author-START##
            Name :: Jonathan Ting
            ##Author-END##
            ##Comment-START##
            Comment :: rAAV-hPTenh(FAM84b)-minBG-SYFP2-WPRE3-BGHpA
                       clones 2,3,4 all good
                       clone 1 low yield
            ##Comment-END##
            ##Imported_From-START##
            Path     :: C:\VNTI Database
            Filename :: _exdata.ma4
            ##Imported_From-END##
FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /label="L-ITR"
     misc_feature    162..553
                     /label="3xhI56i core"
     misc_feature    560..612
                     /label="Beta Globin minimal promoter"
     misc_feature    569..574
                     /label="TATA box"
     misc_feature    complement(575..595)
                     /label="minBG-PCR-R"
     misc_feature    601
                     /label="transcriptional start"
     misc_feature    <637..>1330
                     /Source="referenceToDocument: urn:local:.:u-f2r659a"
                     /label="CN1244_pAAV Linearized (digested by SacI, MluI)
                     Fragment 3 (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    639..1316
                     /Original_Bases="TACCGGTCGCCACCATGGTGAGCAAGGGCGAGGAGCTGTTC
                     ACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCA
                     GCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGCTGAT
                     CTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGGGCTAC
                     GGCGTGCAGTGCTTCGCCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGT
                     CCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAA
                     CTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAG
                     CTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACA
                     ACTACAACAGCCACAACGTCTATATCACCGCCGACAAGCAGAAGAACGGCATCAAGGC
                     CAACTTCAAGATCCGCCACAACATCGAGGACGGCGGCGTGCAGCTCGCCGACCACTAC
                     CAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGA
                     GCTACCAGTCCAAGCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCT
                     GGAGTTCGTGACCGCCGCCGGGATCACTCT"
                     /label="TACCGGTCGCCACCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTG
                     GTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCG
                     GCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGCTGATCTGCACCAC
                     CGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGGGCTACGGCGTGCAG
                     TGCTTCGCCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGC
                     CCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGAC
                     CCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGC
                     ATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACA
                     GCCACAACGTCTATATCACCGCCGACAAGCAGAAGAACGGCATCAAGGCCAACTTCAA
                     GATCCGCCACAACATCGAGGACGGCGGCGTGCAGCTCGCCGACCACTACCAGCAGAAC
                     ACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCTACCAGT
                     CCAAGCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGT
                     GACCGCCGCCGGGATCACTCT"
                     /note="Geneious type: Editing History Replacement"
     gene            643..>1341
                     /modified_by="erikh"
                     /modified_by="refugio.martinez"
                     /Color="#ff0084"
                     /modified_by="ximena.opitzaraya"
                     /label="mScarlet"
     misc_feature    1376..1621
                     /label="WPRE3"
     misc_feature    1376..1535
                     /label="gamma"
     misc_feature    complement(1489..1508)
                     /label="WPRE3-R (JT)"
     misc_feature    1541..1621
                     /label="alpha"
     misc_feature    1635..1838
                     /label="BGH-pA"
     primer          complement(1749..1768)
                     /label="BGH-R"
     misc_feature    1867..2007
                     /label="R-ITR"
     rep_origin      2099..2405
                     /label="f1 Origin"
     misc_feature    2924..3781
                     /label="AmpR"
     rep_origin      3932..4599
                     /label="pUC ori"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtggtac cctaaataaa gatggctttt
      181 tagtattaaa agtggaagaa aattacaggt aattatcttt gacggtaaaa acgctgtaat
      241 cagcgggcta catgaaaaat tactctaatt atggctgcat ttaagagaat ggctaaataa
      301 agatggcttt ttagtattaa aagtggaaga aaattacagg taattatctt tgacggtaaa
      361 aacgctgtaa tcagcgggct acatgaaaaa ttactctaat tatggctgca tttaagagaa
      421 tggctaaata aagatggctt tttagtatta aaagtggaag aaaattacag gtaattatct
      481 ttgacggtaa aaacgctgta atcagcgggc tacatgaaaa attactctaa ttatggctgc
      541 atttaagaga atggagctcg ggctgggcat aaaagtcagg gcagagccat ctattgctta
      601 catttgcttc tgggatccag atctttcgaa gctagcgcca ccatggtgag caagggcgag
      661 gcagtgatca aggagttcat gcggttcaag gtgcacatgg agggctccat gaacggccac
      721 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
      781 ctgaaggtga ccaagggtgg ccccctgccc ttctcctggg acatcctgtc ccctcagttc
      841 atgtacggct ccagggcctt caccaagcac cccgccgaca tccccgacta ctataagcag
      901 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgccgtg
      961 accgtgaccc aggacacctc cctggaggac ggcaccctga tctacaaggt gaagctccgc
     1021 ggcaccaact tccctcctga cggccccgta atgcagaaga agacaatggg ctgggaagcg
     1081 tccaccgagc ggttgtaccc cgaggacggc gtgctgaagg gcgacattaa gatggccctg
     1141 cgcctgaagg acggcggccg ctacctggcg gacttcaaga ccacctacaa ggccaagaag
     1201 cccgtgcaga tgcccggcgc ctacaacgtc gaccgcaagt tggacatcac ctcccacaac
     1261 gaggactaca ccgtggtgga acagtacgaa cgctccgagg gccgccactc caccggcggc
     1321 atggacgagc tgtacaagta agtcgacggc gcgccgcggc cgcgaattcg atatcataat
     1381 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     1441 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     1501 gctttcattt tctcctcctt gtataaatcc tggttagttc ttgccacggc ggaactcatc
     1561 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     1621 gctcgagaga tcttcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     1681 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     1741 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     1801 cagcaagggg gaggattggg aagacaatag caggcatgag atctcacgtg cggaccgagc
     1861 ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca
     1921 ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga
     1981 gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc cttacgcatc
     2041 tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc
     2101 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     2161 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     2221 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     2281 ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     2341 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     2401 aacaacactc aaccctatct cgggctattc ttttgattta taagggattt tgccgatttc
     2461 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     2521 attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     2581 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     2641 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     2701 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     2761 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     2821 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     2881 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     2941 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     3001 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     3061 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     3121 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     3181 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     3241 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     3301 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     3361 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     3421 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     3481 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     3541 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     3601 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     3661 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     3721 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     3781 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     3841 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     3901 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     3961 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     4021 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     4081 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     4141 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     4201 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     4261 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     4321 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     4381 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     4441 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     4501 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     4561 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgt
//
