LOCUS       CN5150_rAAV-3xcore2_eHGT_078h_minBglobin-mTFP1-10aa-H2B-WPRE3-BGHpA        5262 bp    DNA     circular UNA 23-AUG-2023
DEFINITION  Gibson/Homology Cloning ligation product.
ACCESSION   urn.local...c9k-bb65pqx
VERSION     urn.local...c9k-bb65pqx
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     ##Imported_From-START##
            Path     :: \\allen\programs\celltypes\workgroups\0285\CN-VectorNTI
            Filename :: CN2437-rAAV-eHGT_607h-minBglobin-SYFP2-WPRE3-BGHpA.gb
            ##Imported_From-END##
FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /vntifkey=21
                     /locus_tag="L-ITR"
                     /ApEinfo_label="L-ITR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="L-ITR"
     ligation        137..154
                     /label="Ligation"
     misc_feature    137..815
                     /Source="referenceToDocument: urn:local:.:41n-fn0ztff"
                     /label="eHGT_607h.fasta (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     primer_bind     137..155
                     /Mismatches=0
                     /%GC="73.7"
                     /Hairpin_Tm="60.9"
                     /Self_Dimer_Tm="46.9"
                     /Tm="70.6"
                     /annotation_group="3039569766122666ca885a7a-4c46-4b6b-9979
                     -bf25f2d0d94b"
                     /Sequence="TTCCTGCGGCCGCACGCGT"
                     /created_by="primer3"
                     /locus_tag="Gibson Assembly into CN1244 F'"
                     /ApEinfo_label="Gibson Assembly into CN1244 F'"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Gibson Assembly into CN1244 F'"
     misc_feature    138..814
                     /Original_Bases="GAGGCTCTCCCAGATTCTGAAGCTGACCGGCTCACAGACGG
                     CAGTAGTTCTTTGTCGAATGGGGAGCACAGAGCTGGATATGCAGCAGTAAATCATGAC
                     ACAATTATTGAAGCCCAGCCAGTGCCCCCTGGCACATCAGCACAAAAGGCTAAAATCA
                     TTGATCTTACTCAAGCATTAATGTTGGGACAAGGGAAAAAACTTAACATCTATACAGA
                     TTCTAAATATGCATTCCTTGTGGTTCATGCTCATACCGCCATCAGGAAAGAAAGGGGA
                     TTACTTACTAGCAAGCACTCCCCTATAGAGCATGGACCTGAAATTCTGCAGCTATTGA
                     AGGCAATACACCTGCCAAAGGCCATAGCTATCATTCATTGTAGGGGGCATCAAAGGGA
                     CTTAACCCCTATAGCACAAGGGAATGGAAAGGCTGACGGAAGCCAAAGCAAAAGCCCT
                     CAGCGTGCAATCCCAACAGATCCTAGCACTGCTTCCTTTCTACAATTCCCCAGTAGAA
                     CCCAAATACACACCACAGGAAGAACAGCTAATAAAAAAAGCAAGGGAGACAAAAACAA
                     GGATCCTGGTGGCATATGGAGCCAAAAATATATCTCCCTCAGGCAGCCCAGTGGAGAG
                     TTATAAAAGCCCTGCATGACTCTTTCCATGTGGGGAGGGATGCGGCTCTGGCCATGGT
                     AAACAAGTTCTTTACTGGGTTTAACTCAGCTTCAATGGCTAAGCAGGTCTGTCAAGCC
                     TGCTCACTGTGTGCATTTAACAATCCAGGCATCAAAATGCCCCCTCTAATAAAACCAG
                     TCCAAAGGAGGGGAACTTATCCAGGGGAAGACTGGCAGTTAGACGTCATCCATATGCC
                     AGCTTGCAGAGGATACAAGCTTTTGTTAATACTAATAGATACTTTTAGGTTGGGTCAA
                     AGGTTAC"
                     /label="GAGGCTCTCCCAGATTCTGAAGCTGACCGGCTCACAGACGGCAGTAGTTC
                     TTTGTCGAATGGGGAGCACAGAGCTGGATATGCAGCAGTAAATCATGACACAATTATT
                     GAAGCCCAGCCAGTGCCCCCTGGCACATCAGCACAAAAGGCTAAAATCATTGATCTTA
                     CTCAAGCATTAATGTTGGGACAAGGGAAAAAACTTAACATCTATACAGATTCTAAATA
                     TGCATTCCTTGTGGTTCATGCTCATACCGCCATCAGGAAAGAAAGGGGATTACTTACT
                     AGCAAGCACTCCCCTATAGAGCATGGACCTGAAATTCTGCAGCTATTGAAGGCAATAC
                     ACCTGCCAAAGGCCATAGCTATCATTCATTGTAGGGGGCATCAAAGGGACTTAACCCC
                     TATAGCACAAGGGAATGGAAAGGCTGACGGAAGCCAAAGCAAAAGCCCTCAGCGTGCA
                     ATCCCAACAGATCCTAGCACTGCTTCCTTTCTACAATTCCCCAGTAGAACCCAAATAC
                     ACACCACAGGAAGAACAGCTAATAAAAAAAGCAAGGGAGACAAAAACAAGGATCCTGG
                     TGGCATATGGAGCCAAAAATATATCTCCCTCAGGCAGCCCAGTGGAGAGTTATAAAAG
                     CCCTGCATGACTCTTTCCATGTGGGGAGGGATGCGGCTCTGGCCATGGTAAACAAGTT
                     CTTTACTGGGTTTAACTCAGCTTCAATGGCTAAGCAGGTCTGTCAAGCCTGCTCACTG
                     TGTGCATTTAACAATCCAGGCATCAAAATGCCCCCTCTAATAAAACCAGTCCAAAGGA
                     GGGGAACTTATCCAGGGGAAGACTGGCAGTTAGACGTCATCCATATGCCAGCTTGCAG
                     AGGATACAAGCTTTTGTTAATACTAATAGATACTTTTAGGTTGGGTCAAAGGTTAC"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    156..791
                     /created_by="ximena.opitzaraya"
                     /label="3xcore2_eHGT_078h"
     primer_bind     complement(792..815)
                     /Mismatches=0
                     /%GC="58.3"
                     /Hairpin_Tm="51.4"
                     /Self_Dimer_Tm="2.7"
                     /Tm="65.8"
                     /annotation_group="29463186574981419038fb14-2e20-4d98-85ef
                     -510a13f70738"
                     /Sequence="GACTTTTATGCCCAGCCCGAGCTC"
                     /created_by="primer3"
                     /locus_tag="Gibson Assembly into CN1244 R'"
                     /ApEinfo_label="Gibson Assembly into CN1244 R'"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Gibson Assembly into CN1244 R'"
     ligation        797..815
                     /label="Ligation"
     misc_feature    797..154
                     /Source="referenceToDocument: urn:local:.:41m-fn0ztff"
                     /label="CN1244_pAAV Linearized (digested by SacI, MluI)
                     Fragment 3 (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    798..850
                     /vntifkey=21
                     /locus_tag="Beta\Globin\minimal\promoter"
                     /ApEinfo_label="Beta\Globin\minimal\promoter"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Beta\Globin\minimal\promoter"
     misc_feature    807..812
                     /vntifkey=21
                     /locus_tag="TATA\box"
                     /ApEinfo_label="TATA\box"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="TATA\box"
     misc_feature    839
                     /vntifkey=21
                     /locus_tag="transcriptional\start"
                     /ApEinfo_label="transcriptional\start"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="transcriptional\start"
     misc_feature    <875..>1586
                     /Source="referenceToDocument: urn:local:.:41o-fn0ztff"
                     /label="CN1244_pAAV Linearized (digested by SacI, MluI)
                     Fragment 3 (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    <875..>1586
                     /Source="referenceToDocument: urn:local:.:41p-fn0ztff"
                     /label="CN1244_pAAV Linearized (digested by SacI, MluI)
                     Fragment 3 (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    877..1998
                     /Original_Bases="TACCGGTCGCCACCATGGTGAGCAAGGGCGAGGAGCTGTTC
                     ACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCA
                     GCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGCTGAT
                     CTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGGGCTAC
                     GGCGTGCAGTGCTTCGCCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGT
                     CCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAA
                     CTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAG
                     CTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACA
                     ACTACAACAGCCACAACGTCTATATCACCGCCGACAAGCAGAAGAACGGCATCAAGGC
                     CAACTTCAAGATCCGCCACAACATCGAGGACGGCGGCGTGCAGCTCGCCGACCACTAC
                     CAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGA
                     GCTACCAGTCCAAGCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCT
                     GGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTA"
                     /label="TACCGGTCGCCACCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTG
                     GTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCG
                     GCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGCTGATCTGCACCAC
                     CGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGGGCTACGGCGTGCAG
                     TGCTTCGCCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGC
                     CCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGAC
                     CCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGC
                     ATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACA
                     GCCACAACGTCTATATCACCGCCGACAAGCAGAAGAACGGCATCAAGGCCAACTTCAA
                     GATCCGCCACAACATCGAGGACGGCGGCGTGCAGCTCGCCGACCACTACCAGCAGAAC
                     ACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCTACCAGT
                     CCAAGCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGT
                     GACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTA"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    881..>1586
                     /label="mTFP1"
     misc_feature    1589..1618
                     /vntifkey=21
                     /label="10\a.a.\linker"
     misc_feature    1619..1993
                     /vntifkey=21
                     /label="H2B"
     misc_feature    complement(1974..1996)
                     /vntifkey=21
                     /label="H2B-R"
     misc_feature    2034..2279
                     /vntifkey=21
                     /locus_tag="WPRE3"
                     /ApEinfo_label="WPRE3"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="WPRE3"
     misc_feature    2293..2496
                     /vntifkey=21
                     /locus_tag="BGH-pA"
                     /ApEinfo_label="BGH-pA"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="BGH-pA"
     primer          complement(2407..2426)
                     /vntifkey=27
                     /locus_tag="BGH-R"
                     /ApEinfo_label="BGH-R"
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#80ff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="BGH-R"
     misc_feature    2525..2665
                     /vntifkey=21
                     /locus_tag="R-ITR"
                     /ApEinfo_label="R-ITR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="R-ITR"
     rep_origin      2757..3063
                     /vntifkey=33
                     /locus_tag="f1\Origin"
                     /ApEinfo_label="f1\Origin"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="f1\Origin"
     misc_feature    3582..4439
                     /vntifkey=21
                     /locus_tag="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="AmpR"
     rep_origin      4590..5257
                     /vntifkey=33
                     /locus_tag="pUC\ori"
                     /ApEinfo_label="pUC\ori"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="pUC\ori"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtattag caatttggca ggtacccgag
      181 ggccatacct aatctgcata aaatgaagca gattgcaacc gccctcatct tttttatttt
      241 taaactggtt tttgaagcag agcataaaat ctcagaggga gagacagaag atgctagtgc
      301 atacattttc cttcatgcct ttattttcat tctttttgca caaaccatct tcctgaatgg
      361 ctgtttaatt agcaatttgg caggtacccg agggccatac ctaatctgca taaaatgaag
      421 cagattgcaa ccgccctcat cttttttatt tttaaactgg tttttgaagc agagcataaa
      481 atctcagagg gagagacaga agatgctagt gcatacattt tccttcatgc ctttattttc
      541 attctttttg cacaaaccat cttcctgaat ggctgtttaa ttagcaattt ggcaggtacc
      601 cgagggccat acctaatctg cataaaatga agcagattgc aaccgccctc atctttttta
      661 tttttaaact ggtttttgaa gcagagcata aaatctcaga gggagagaca gaagatgcta
      721 gtgcatacat tttccttcat gcctttattt tcattctttt tgcacaaacc atcttcctga
      781 atggctgttt agagctcggg ctgggcataa aagtcagggc agagccatct attgcttaca
      841 tttgcttctg ggatccagat ctttcgaagc tagcgccacc atggtgagca agggcgagga
      901 gaccacaatg ggcgtaatca agcccgacat gaagatcaag ctgaagatgg agggcaacgt
      961 gaatggccac gccttcgtga tcgagggcga gggcgagggc aagccctacg acggcaccaa
     1021 caccatcaac ctggaggtga aggagggagc ccccctgccc ttctcctacg acattctgac
     1081 caccgcgttc gcctacggca acagggcctt caccaagtac cccgacgaca tccccaacta
     1141 cttcaagcag tccttccccg agggctactc ttgggagcgc accatgacct tcgaggacaa
     1201 gggcatcgtg aaggtgaagt ccgacatctc catggaggag gactccttca tctacgagat
     1261 acacctcaag ggcgagaact tcccccccaa cggccccgtg atgcagaaga agaccaccgg
     1321 ctgggacgcc tccaccgaga ggatgtacgt gcgcgacggc gtgctgaagg gcgacgtcaa
     1381 gcacaagctg ctgctggagg gcggcggcca ccaccgcgtt gacttcaaga ccatctacag
     1441 ggccaagaag gcggtgaagc tgcccgacta tcactttgtg gaccaccgca tcgagatcct
     1501 gaaccacgac aaggactaca acaaggtgac cgtttacgag agcgccgtgg cccgcaactc
     1561 caccgacggc atggacgagc tgtacaagtc cggactcaga tctggaggct ccggaggccc
     1621 agagccagcg aagtctgctc ccgccccgaa aaagggctcc aagaaggcgg tgactaaggc
     1681 gcagaagaaa ggcggcaaga agcgcaagcg cagccgcaag gagagctatt ccatctatgt
     1741 gtataaggtt ctgaagcagg tccaccctga caccggcatt tcgtccaagg ccatgggcat
     1801 catgaactcg tttgtgaacg acattttcga gcgcatcgca ggtgaggctt cccgcctggc
     1861 gcattacaac aagcgctcga ccatcacctc cagggagatc cagacggccg tgcgcctgct
     1921 gctgcctggg gagttggcca agcacgccgt gtccgagggt actaaggcca tcaccaagta
     1981 caccagcgct aagtaatgag tcgacggcgc gccgcggccg cgaattcgat atcataatca
     2041 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     2101 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     2161 tttcattttc tcctccttgt ataaatcctg gttagttctt gccacggcgg aactcatcgc
     2221 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggc
     2281 tcgagagatc ttcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     2341 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     2401 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     2461 gcaaggggga ggattgggaa gacaatagca ggcatgagat ctcacgtgcg gaccgagcgg
     2521 ccgcaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact
     2581 gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc
     2641 gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt attttctcct tacgcatctg
     2701 tgcggtattt cacaccgcat acgtcaaagc aaccatagta cgcgccctgt agcggcgcat
     2761 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     2821 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     2881 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     2941 ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     3001 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     3061 caacactcaa ccctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg
     3121 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     3181 taacgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     3241 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     3301 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     3361 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
     3421 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     3481 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     3541 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     3601 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     3661 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     3721 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     3781 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     3841 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     3901 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     3961 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     4021 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     4081 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     4141 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     4201 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     4261 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     4321 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     4381 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     4441 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     4501 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     4561 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     4621 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     4681 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     4741 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     4801 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     4861 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     4921 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     4981 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     5041 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     5101 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     5161 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     5221 ttttacggtt cctggccttt tgctggcctt ttgctcacat gt
//
