LOCUS       Exported                6332 bp ds-DNA     circular SYN 16-APR-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6332)
  AUTHORS   EMBL Monterotondo
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Nov 12, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6332
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(18..39)
                     /label=1.4
                     /note="color: black; sequence: cacctccccctgaacctgaaac;
                     added: 2021-04-12"
     primer_bind     18..39
                     /label=3.7
                     /note="color: black; sequence: GTTTCAGGTTCAGGGGGAGGTG;
                     added: 2024-04-15"
     CDS             complement(40..1065)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /note="color: #ccffcc"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     821..863
                     /label=2.5
                     /note="color: black; sequence: 
                     tccccaatgtcaagcacttccggaatcgggagcgcggccgatg; added: 
                     2024-04-15"
     primer_bind     complement(823..845)
                     /label=2.6
                     /note="color: black; sequence: ttccggaagtgcttgacattggg;
                     added: 2024-04-15"
     CDS             complement(1072..1125)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /note="color: #cc99b2
                     Cleavage site after base 1074"
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(1073..1096)
                     /label=1.54
                     /note="color: black; sequence: 
                     tgacgtcgaggagaatcctggcccaGTGAGCAAGGGCGAGGAGC; added: 
                     2020-11-26"
     primer_bind     1125..1138
                     /label=1.52
                     /note="color: black; sequence: 
                     tatgtttcaggttcagggggaggtgTTATCTAGATCCGGTGGATCCTACC; added: 
                     2020-11-20"
     CDS             complement(1135..1155)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     CDS             complement(1159..1179)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     CDS             complement(1183..1203)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     primer_bind     complement(1206..1227)
                     /label=3.85
                     /note="color: black; sequence: TCCGGACTCAGATCTCGAGCTG;
                     added: 2024-04-15"
     CDS             complement(1228..1926)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /note="color: #3366ff"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     primer_bind     1228..1243
                     /label=3.80
                     /note="color: black; sequence: 
                     actagtgagctcgtcgacgtaggCTAGGATCCATTAAGCTTGTGCCCC; added: 
                     2024-04-15"
     primer_bind     complement(1944..1991)
                     /label=1.51
                     /note="color: black; sequence: 
                     ccactgcttactggcttatcgaaatataACCGTCAGATCCGCTAGCGC; added: 
                     2020-11-20"
     primer_bind     1949..1972
                     /label=1.98
                     /note="color: black; sequence: AGCGGATCTGACGGTtatatttcg;
                     added: 2024-04-15"
     primer_bind     1964..1991
                     /label=MP1.6
                     /note="color: black; sequence: 
                     tccgcgcccgatggtgggacggtatatttcgataagccagtaagcagtgg; added: 
                     2020-09-25"
     primer_bind     complement(1965..1991)
                     /label=1.48
                     /note="color: black; sequence: 
                     CCACTGCTTACTGGCTTATCGAAATatgaccgagtacaagcccacg; added: 
                     2021-04-12"
     primer_bind     1967..1991
                     /label=1.7
                     /note="color: black; sequence: atttcgataagccagtaagcagtgg;
                     added: 2020-11-20"
     primer_bind     complement(1986..2006)
                     /label=pcDNA3_for
                     /note="color: black; sequence: GGCTAACTAGAGAACCCACTG;
                     added: 2021-10-18"
     promoter        complement(2011..2511)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(2191..2210)
                     /label=SEQA
                     /note="color: black; sequence: GATGGGCTGTGGCCAATAGC; added:
                     2021-10-26"
     misc_feature    2393..4222
                     /label=PCR1
                     /note="color: #a6acb3"
     primer_bind     2393..2414
                     /label=4.3
                     /note="color: black; sequence: CTGCGAACGGACGTGAAGAATG;
                     added: 2024-04-15"
     misc_feature    2516..2535
                     /label=sgRNA ActB_FA
                     /note="color: #a6acb3; direction: RIGHT
                     Cleavage site after base 2532"
     primer_bind     2516..2535
                     /label=2.78
                     /note="color: black; sequence: CACCGACAGCTCCCCACACACCAC;
                     added: 2024-04-15"
     CDS             2752..2805
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /note="color: #cc99b2
                     Cleavage site after base 2802"
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     2781..2804
                     /label=1.54
                     /note="color: black; sequence: 
                     tgacgtcgaggagaatcctggcccaGTGAGCAAGGGCGAGGAGC; added: 
                     2020-11-26"
     regulatory      2812..2821
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
     CDS             2818..3525
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /note="color: #ff0000"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3062..3081)
                     /label=3.13
                     /note="color: black; sequence: CAAGTAGTCGGGGATGTCGG; added:
                     2024-04-15"
     primer_bind     complement(3504..3525)
                     /label=1.38
                     /note="color: black; sequence: 
                     CGACAAGTGAGCTCGTCGACGTAGGttagactttcctcttcttcttgggagaaccacca
                      gaCTTGTACAGCTCGTCCATGCCG; added: 2024-04-15"
     primer_bind     complement(3504..3525)
                     /label=2.89
                     /note="color: black; sequence: 
                     TATCTATGCGGCCGCACTAGTATTTttacttgtacagctcgtccatgccg; added: 
                     2024-04-15"
     primer_bind     complement(3505..3527)
                     /label=3.43
                     /note="color: black; sequence: 
                     CCCTGAAAATACAGGTTTTCtccgctgcccttgtacagctcgtccatgcc; added: 
                     2024-04-15"
     primer_bind     complement(3505..3525)
                     /label=1.9
                     /note="color: black; sequence: 
                     tatgtttcaggttcagggggaggtgttacttgtacagctcgtccatgcc; added: 
                     2021-04-12"
     primer_bind     complement(3505..3525)
                     /label=3.74
                     /note="color: black; sequence: 
                     ctagtgagctcgtcgacgtaggTTACTTGTACAGCTCGTCCATGCC; added: 
                     2024-04-15"
     CDS             3541..3597
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /note="color: #cc99b2
                     Cleavage site after base 3594"
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     3562..3621
                     /label=1.66
                     /note="color: black; sequence: 
                     gcctgctgaagcaggctggagacgtggaggagaaccctggacctatgaccgagtacaag
                      cccacg; added: 2021-04-12"
     primer_bind     3571..3622
                     /label=1.82
                     /note="color: black; sequence: 
                     gcggagagggcagaggaagtcttctaacatgcggtgacgtggaggagaatcccggccct
                      agcaccgagtacaagcccacgg; added: 2021-04-12"
     primer_bind     3571..3621
                     /label=1.71
                     /note="color: black; sequence: 
                     aagcttctgacctcttctcttcctcccacagggcctcgagagatctggcagcggagagg
                      
                     gcagaggaagtcttctaacatgcggtgacgtggaggagaatcccggccctaggctcgag
                      atgaccgagtacaagcccacg; added: 2021-02-15"
     primer_bind     3571..3621
                     /label=1.71bis
                     /note="color: black; sequence: 
                     ctggcagcggagagggcagaggaagtcttctaacatgcggtgacgtggaggagaatccc
                      ggccctaggctcgagatgaccgagtacaagcccacg; added: 2021-03-09"
     primer_bind     complement(3583..3625)
                     /label=2.72
                     /note="color: black; sequence: 
                     gcaccgtgggcttgtactcggtcatcccgGGACCGGGGTTTTC; added: 
                     2022-10-18"
     misc_feature    3601..4485
                     /label=form psBIG1a SA T2A puro...#1
                     /note="color: #a6acb3"
     CDS             3601..4200
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /note="color: #ccffcc"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     3601..3621
                     /label=1.48
                     /note="color: black; sequence: 
                     CCACTGCTTACTGGCTTATCGAAATatgaccgagtacaagcccacg; added: 
                     2021-04-12"
     primer_bind     3601..3621
                     /label=2.73
                     /note="color: black; sequence: atgaccgagtacaagcccacg;
                     added: 2022-10-18"
     primer_bind     3601..3621
                     /label=2.10
                     /note="color: black; sequence: 
                     atacattatacgaagttatctggttatgaccgagtacaagcccacg; added: 
                     2024-04-15"
     primer_bind     3604..3618
                     /label=3.51
                     /note="color: black; sequence: 
                     AGAGACAGGATGAGGATCGTTTCGCATGGGTACCGAGTACAAGCCC; added: 
                     2024-04-15"
     primer_bind     complement(4074..4092)
                     /label=1.84
                     /note="color: black; sequence: CGCGGAGGTCTCCAGGAAG; added: 
                     2024-04-15"
     primer_bind     4093..4110
                     /label=1.83
                     /note="color: black; sequence: CCCCGCAACCTCCCCTTC; added: 
                     2024-04-15"
     primer_bind     complement(4181..4225)
                     /label=1.10
                     /note="color: black; sequence: 
                     tatgtttcaggttcagggggaggtgtcaggcaccgggcttgcggg; added: 
                     2021-04-12"
     primer_bind     complement(4200..4225)
                     /label=1.9
                     /note="color: black; sequence: 
                     tatgtttcaggttcagggggaggtgttacttgtacagctcgtccatgcc; added: 
                     2021-04-12"
     primer_bind     complement(4200..4225)
                     /label=1.52
                     /note="color: black; sequence: 
                     tatgtttcaggttcagggggaggtgTTATCTAGATCCGGTGGATCCTACC; added: 
                     2020-11-20"
     primer_bind     4201..4222
                     /label=1.4
                     /note="color: black; sequence: cacctccccctgaacctgaaac;
                     added: 2021-04-12"
     primer_bind     complement(4201..4222)
                     /label=3.7
                     /note="color: black; sequence: GTTTCAGGTTCAGGGGGAGGTG;
                     added: 2024-04-15"
     polyA_signal    4249..4383
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(4372..4408)
                     /label=1.73
                     /note="color: black; sequence: 
                     tatcgaggctaactgaaacacggaaCAAGCAGTGATCAGATCCAGACATG; added: 
                     2021-02-15"
     primer_bind     complement(4372..4384)
                     /label=1.61
                     /note="color: black; sequence: 
                     ggttatgatagttattgctcagcgCTCAAGCAGTGATCAGATCCAGACATG; added:
                     2021-04-12"
     primer_bind     complement(4372..4384)
                     /label=1.32
                     /note="color: black; sequence: 
                     cagagtgccagccctgggaccgaacCAAGCAGTGATCAGATCCAGACATG; added: 
                     2021-04-12"
     primer_bind     4384..4405
                     /label=1.44
                     /note="color: black; sequence: ttccgtgtttcagttagcctcg;
                     added: 2020-10-22"
     misc_feature    4410..4432
                     /label=bigbac_A
                     /note="color: #00ccff"
     primer_bind     4437..4467
                     /label=2.9
                     /note="color: black; sequence: 
                     aaccagataacttcgtataatgtatgctatacg; added: 2024-04-15"
     primer_bind     complement(4437..4459)
                     /label=2.10
                     /note="color: black; sequence: 
                     atacattatacgaagttatctggttatgaccgagtacaagcccacg; added: 
                     2024-04-15"
     protein_bind    complement(4441..4474)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
                     /note="color: #31849b; direction: LEFT"
     primer_bind     complement(4460..4509)
                     /label=2.74
                     /note="color: black; sequence: 
                     tatgtcaactgggttcgtgccttcgccgcggtaccataacttcgtatagc; added: 
                     2022-10-18"
     misc_feature    4486..5275
                     /label=form psBIG1a SA T2A puro...#2
                     /note="color: #a6acb3"
     primer_bind     4486..4506
                     /label=2.75
                     /note="color: black; sequence: gaaggcacgaacccagttgac;
                     added: 2022-10-18"
     promoter        4502..4530
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
                     /note="This forward directional feature has 3 segments:
                     1: 4502 .. 4507 / #ffffff / -35
                     2: 4508 .. 4524 / #ffffff
                     3: 4525 .. 4530 / #ffffff / -10"
     CDS             4719..5252
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /note="color: #ccffcc"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     primer_bind     5253..5298
                     /label=2.77
                     /note="color: black; sequence: 
                     caattcgttcaagccgagatcggggccaggaaccgtaaaaaggccg; added: 
                     2022-10-18"
     primer_bind     complement(5253..5275)
                     /label=2.76
                     /note="color: black; sequence: ccgatctcggcttgaacgaattg;
                     added: 2022-10-18"
     misc_feature    5276..5953
                     /label=form psBIG1a SA T2A puro...#3
                     /note="color: #a6acb3"
     rep_origin      complement(5312..5900)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     primer_bind     complement(5929..5953)
                     /label=1.2
                     /note="color: black; sequence: gagttttcgttccactgagcgtcag;
                     added: 2021-04-12"
     primer_bind     5930..5975
                     /label=2.71
                     /note="color: black; sequence: 
                     tgacgctcagtggaacgaaaactccggatctcctgtttaaaccccg; added: 
                     2022-10-18"
     misc_feature    join(5954..6332,1..3600)
                     /label=ORDER
                     /note="color: #a6acb3"
     misc_feature    5968..5990
                     /label=bigbac_B
                     /note="color: #3366ff"
     primer_bind     complement(6138..6163)
                     /label=1.68
                     /note="color: black; sequence: aaatctatatctcaatcggggttacg; 
                     added: 2021-04-12"
     misc_feature    6141..6163
                     /label=bigbac_omega
                     /note="color: #ff0000"
     primer_bind     6164..6187
                     /label=1.67
                     /note="color: black; sequence: aaatctttagaccatagagcgttc;
                     added: 2021-04-12"
     misc_feature    6164..6186
                     /label=bigbac_alpha
                     /note="color: #ffff00"
     primer_bind     6194..6243
                     /label=1.70
                     /note="color: black; sequence: 
                     cagagtgccagccctgggaccgaacTCTTCTAGGCTCAAGCAGTGATCAG; added: 
                     2021-02-15"
     primer_bind     6194..6218
                     /label=1.32
                     /note="color: black; sequence: 
                     cagagtgccagccctgggaccgaacCAAGCAGTGATCAGATCCAGACATG; added: 
                     2021-04-12"
     primer_bind     complement(6201..6218)
                     /label=1.23
                     /note="color: black; sequence: gttcggtcccagggctgg; added: 
                     2020-10-01"
     polyA_signal    6243..6324
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
ORIGIN
        1 caattgcatt catttttgtt tcaggttcag ggggaggtgc tattcctttg ccctcggacg
       61 agtgctgggg cgtcggtttc cactatcggc gagtacttct acacagccat cggtccagac
      121 ggccgcgctt ctgcgggcga tttgtgtacg cccgacagtc ccggctccgg atcggacgat
      181 tgcgtcgcat cgaccctgcg cccaagctgc atcatcgaaa ttgccgtcaa ccaagctctg
      241 atagagttgg tcaagaccaa tgcggagcat atacgcccgg agccgcggcg atcctgcaag
      301 ctccggatgc ctccgctcga agtagcgcgt ctgctgctcc atacaagcca accacggcct
      361 ccagaagaag atgttggcga cctcgtattg ggaatccccg aacatcgcct cgctccagtc
      421 aatgaccgct gttatgcggc cattgtccgt caggacattg ttggagccga aatccgcgtg
      481 cacgaggtgc cggacttcgg ggcagtcctc ggcccaaagc atcagctcat cgagagcctg
      541 cgcgacggac gcactgacgg tgtcgtccat cacagtttgc cagtgataca catggggatc
      601 agcaatcgcg catatgaaat cacgccatgt agtgtattga ccgattcctt gcggtccgaa
      661 tgggccgaac ccgctcgtct ggctaagatc ggccgcagcg atcgcatcca tggcctccgc
      721 gaccggctgc agaacagcgg gcagttcggt ttcaggcagg tcttgcaacg tgacaccctg
      781 tgcacggcgg gagatgcaat aggtcaggct ctcgctgaat tccccaatgt caagcacttc
      841 cggaatcggg agcgcggccg atgcaaagtg ccgataaaca taacgatctt tgtagaaacc
      901 atcggcgcag ctatttaccc gcaggacata tccacgccct cctacatcga agctgaaagc
      961 acgagattct tcgccctccg agagctgcat caggtcggag acgctgtcga acttttcgat
     1021 cagaaacttc tcgacagacg tcgcggtgag ttcaggcttt ttcatgtata cggggccagg
     1081 attctcctcg acgtcaccgc atgttagcag acttcctctg ccctcggtgg atcctacctt
     1141 tctcttcttt tttggatcta cctttctctt cttttttgga tctacctttc tcttcttttt
     1201 tggatcagct cgagatctga gtccggaatt aagcttgtgc cccagtttgc tagggaggtc
     1261 gcagtatctg gccactgcca cctcgtgctg ctcgacgtag gtctcgttgt tggcctcctt
     1321 gattctttcc agtctgtagt ccacatagta gacgccaggc atcttgaggt tcttagcggg
     1381 tttcttggat ctatatgtgg tcttgatgtt tgcgatcaga tggctcccgc ccacgagctt
     1441 cagggccatg tcgtttctgc cttccaggcc gccgtcagcg gggtacagcg tctcggtgaa
     1501 ggcctcccag ccgagtgttt tcttctgcat cacagggccg ttggatgtga agttcacccc
     1561 tctgatcttg acgttgtaga tgaggcagcc gtcctggagg ctggtgtcct gggtagcggt
     1621 cagcacgccc ccgtcttcgt atgtggtgac tctctcccat gtgaagccct cagggaagga
     1681 ctgcttgaag aagtcgggga tgccctgggt gtggttgatg aaggtcttgc tgccgtagag
     1741 gaagctagta gccaggatgt cgaaggcgaa ggggagaggg ccgccctcga ccaccttgat
     1801 tctcatggtc tgggtgccct cgtagggctt gccttcgccc tcggatgtgc acttgaagtg
     1861 atggttgtcc acggtgccct ccatgtacag cttcatgtgc atgttctcct taatcagctc
     1921 gctcatggtg gcgaccggta tacgcgctag cggatctgac ggttatattt cgataagcca
     1981 gtaagcagtg ggttctctag ttagccagag ctggggagag aggtcggtga ttcggtcaac
     2041 gagggagccg actgccgacg tgcgctccgg aggcttgcag aatgcggaac accgcgcggg
     2101 caggaacagg gcccacacta ccgccccaca ccccgcctcc cgcaccgccc cttcccggcc
     2161 gctgctctcg gcgcgccctg ctgagcagcc gctattggcc acagcccatc gcggtcggcg
     2221 cgctgccatt gctccctggc gctgtccgtc tgcgagggta ctagtgagac gtgcggcttc
     2281 cgtttgtcac gtccggcacg ccgcgaaccg caaggaacct tcccgactta ggggcggagc
     2341 aggaagcgtc gccggggggc ccacaagggt agcggcgaag atccgggtga cgctgcgaac
     2401 ggacgtgaag aatgtgcgag acccagggtc ggcgccgctg cgtttcccgg aaccacgccc
     2461 agagcagccg cgtccctgcg caaacccagg gctgccttgg aaaaggcgca aatgagacag
     2521 ctccccacac accacagggt gtgtggggag ctgtcacatc cagggtcctc actgcctgtc
     2581 cccttccctc ctcagatcat tgctcctcct gagcgcaagt actccgtgtg gatcggcggc
     2641 tccatcctgg cctcgctgtc caccttccag cagatgtgga tcagcaagca ggagtatgac
     2701 gagtccggcc cctccatcgt ccaccgcaaa tgcttcgggg ccggatcggg agagggcaga
     2761 ggaagtctgc taacatgcgg tgacgtcgag gagaatcctg gccccggggt cgccaccatg
     2821 gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg
     2881 cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
     2941 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc
     3001 gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc
     3061 gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg
     3121 atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc
     3181 gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg
     3241 cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc
     3301 ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag
     3361 gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac
     3421 atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc
     3481 gccgagggcc gccactccac cggcggcatg gacgagctgt acaagggggc cggatcggga
     3541 gccacgaact tctctctgtt aaagcaagca ggagacgtgg aagaaaaccc cggtcccggg
     3601 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
     3661 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
     3721 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     3781 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
     3841 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     3901 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     3961 cccgcgtggt tcctggccac cgtcggagtc tcgcccgacc accagggcaa gggtctgggc
     4021 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     4081 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     4141 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
     4201 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     4261 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     4321 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     4381 atcttccgtg tttcagttag cctcgataaa aactttagac catagagcgt ttaaacccag
     4441 ataacttcgt ataatgtatg ctatacgaag ttatggtacc gcggcgaagg cacgaaccca
     4501 gttgacataa gcctgttcgg ttcgtaaact gtaatgcaag tagcgtatgc gctcacgcaa
     4561 ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc
     4621 ttgttatgac tgtttttttg tacagtctat gcctcgggca tccaagcagc aagcgcgtta
     4681 cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc agcaacgatg
     4741 ttacgcagca gggcagtcgc cctaaaacaa agttaggtgg ctcaagtatg ggcatcattc
     4801 gcacatgtag gctcggccct gaccaagtca aatccatgcg ggctgctctt gatcttttcg
     4861 gtcgtgagtt cggagacgta gccacctact cccaacatca gccggactcc gattacctcg
     4921 ggaacttgct ccgtagtaag acattcatcg cgcttgctgc cttcgaccaa gaagcggttg
     4981 ttggcgctct cgcggcttac gttctgccca agtttgagca gccgcgtagt gagatctata
     5041 tctatgatct cgcagtctcc ggcgagcacc ggaggcaggg cattgccacc gcgctcatca
     5101 atctcctcaa gcatgaggcc aacgcgcttg gtgcttatgt gatctacgtg caagcagatt
     5161 acggtgacga tcccgcagtg gctctctata caaagttggg catacgggaa gaagtgatgc
     5221 actttgatat cgacccaagt accgccacct aacaattcgt tcaagccgag atcggggcca
     5281 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     5341 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     5401 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     5461 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     5521 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     5581 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     5641 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     5701 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     5761 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     5821 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     5881 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     5941 ggaacgaaaa ctccggatct cctgtttaaa ccccgattga gatatagttt ttaattaacc
     6001 cgtagtggct atggcagggc ttgccgcccc gacgttggct gcgagccctg ggccttcacc
     6061 cgaacttggg ggttggggtg gggaaaagga agaaacgcgg gcgtattggt cccaatgggg
     6121 tctcggtggg gtatcgacgt aaccccgatt gagatataga tttaaatctt tagaccatag
     6181 agcgttcact tgacagagtg ccagccctgg gaccgaactc ttctaggctc aagcagtgat
     6241 cagcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     6301 attataagct gcaataaaca agttaacaac aa
//
