LOCUS       Exported                6304 bp ds-DNA     circular SYN 10-JAN-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6304)
  AUTHORS   EMBL Monterotondo
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Nov 12, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6304
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     1..26
                     /label=1.68
                     /note="color: black; sequence: aaatctatatctcaatcggggttacg; 
                     added: 2021-04-12"
     misc_feature    1..23
                     /label=bigbac_omega
                     /note="color: #ff0000"
     misc_feature    174..196
                     /label=bigbac_B
                     /note="color: #3366ff"
     primer_bind     complement(189..234)
                     /label=2.71
                     /note="color: black; sequence: 
                     tgacgctcagtggaacgaaaactccggatctcctgtttaaaccccg; added: 
                     2022-10-18"
     primer_bind     211..235
                     /label=1.2
                     /note="color: black; sequence: gagttttcgttccactgagcgtcag;
                     added: 2021-04-12"
     rep_origin      264..852
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     primer_bind     complement(866..911)
                     /label=2.77
                     /note="color: black; sequence: 
                     caattcgttcaagccgagatcggggccaggaaccgtaaaaaggccg; added: 
                     2022-10-18"
     primer_bind     889..911
                     /label=2.76
                     /note="color: black; sequence: ccgatctcggcttgaacgaattg;
                     added: 2022-10-18"
     CDS             complement(912..1445)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /note="color: #ccffcc"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(1634..1662)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
                     /note="This reverse directional feature has 3 segments:
                     1: 1634 .. 1639 / #ffffff / -10
                     2: 1640 .. 1656 / #ffffff
                     3: 1657 .. 1662 / #ffffff / -35"
     primer_bind     1655..1704
                     /label=2.74
                     /note="color: black; sequence: 
                     tatgtcaactgggttcgtgccttcgccgcggtaccataacttcgtatagc; added: 
                     2022-10-18"
     primer_bind     complement(1658..1678)
                     /label=2.75
                     /note="color: black; sequence: gaaggcacgaacccagttgac;
                     added: 2022-10-18"
     protein_bind    1690..1723
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
                     /note="color: #31849b; direction: RIGHT"
     misc_feature    1732..1754
                     /label=bigbac_A
                     /note="color: #00ccff"
     primer_bind     1756..1792
                     /label=1.73
                     /note="color: black; sequence: 
                     tatcgaggctaactgaaacacggaaCAAGCAGTGATCAGATCCAGACATG; added: 
                     2021-02-15"
     primer_bind     complement(1759..1780)
                     /label=1.44
                     /note="color: black; sequence: ttccgtgtttcagttagcctcg;
                     added: 2020-10-22"
     primer_bind     1780..1792
                     /label=1.61
                     /note="color: black; sequence: 
                     ggttatgatagttattgctcagcgCTCAAGCAGTGATCAGATCCAGACATG; added:
                     2021-04-12"
     primer_bind     1780..1792
                     /label=1.32
                     /note="color: black; sequence: 
                     cagagtgccagccctgggaccgaacCAAGCAGTGATCAGATCCAGACATG; added: 
                     2021-04-12"
     polyA_signal    1781..1915
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     1939..1983
                     /label=1.10
                     /note="color: black; sequence: 
                     tatgtttcaggttcagggggaggtgtcaggcaccgggcttgcggg; added: 
                     2021-04-12"
     primer_bind     1939..1964
                     /label=1.9
                     /note="color: black; sequence: 
                     tatgtttcaggttcagggggaggtgttacttgtacagctcgtccatgcc; added: 
                     2021-04-12"
     primer_bind     1939..1964
                     /label=1.52
                     /note="color: black; sequence: 
                     tatgtttcaggttcagggggaggtgTTATCTAGATCCGGTGGATCCTACC; added: 
                     2020-11-20"
     primer_bind     complement(1942..1963)
                     /label=1.4
                     /note="color: black; sequence: cacctccccctgaacctgaaac;
                     added: 2021-04-12"
     CDS             complement(1964..2563)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /note="color: #ccffcc"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2542..2593)
                     /label=1.82
                     /note="color: black; sequence: 
                     gcggagagggcagaggaagtcttctaacatgcggtgacgtggaggagaatcccggccct
                      agcaccgagtacaagcccacgg; added: 2021-04-12"
     primer_bind     complement(2543..2602)
                     /label=1.66
                     /note="color: black; sequence: 
                     gcctgctgaagcaggctggagacgtggaggagaaccctggacctatgaccgagtacaag
                      cccacg; added: 2021-04-12"
     primer_bind     complement(2543..2593)
                     /label=1.71
                     /note="color: black; sequence: 
                     aagcttctgacctcttctcttcctcccacagggcctcgagagatctggcagcggagagg
                      
                     gcagaggaagtcttctaacatgcggtgacgtggaggagaatcccggccctaggctcgag
                      atgaccgagtacaagcccacg; added: 2021-02-15"
     primer_bind     complement(2543..2593)
                     /label=1.71bis
                     /note="color: black; sequence: 
                     ctggcagcggagagggcagaggaagtcttctaacatgcggtgacgtggaggagaatccc
                      ggccctaggctcgagatgaccgagtacaagcccacg; added: 2021-03-09"
     primer_bind     complement(2543..2563)
                     /label=1.48
                     /note="color: black; sequence: 
                     CCACTGCTTACTGGCTTATCGAAATatgaccgagtacaagcccacg; added: 
                     2021-04-12"
     primer_bind     complement(2543..2563)
                     /label=2.73
                     /note="color: black; sequence: atgaccgagtacaagcccacg;
                     added: 2022-10-18"
     CDS             complement(2567..2623)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /note="color: #cc99b2
                     Cleavage site after base 2569"
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(2624..3367)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /note="This feature has 8 segments:
                     1: 2624 .. 2629 / #cc99b2
                     2: 2630 .. 2665 / #993366 / VP16 minimal transcriptio... 3:
                     2666 .. 2668 / #cc99b2
                     4: 2669 .. 2704 / #993366 / VP16 minimal transcriptio... 5:
                     2705 .. 2707 / #cc99b2
                     6: 2708 .. 2743 / #993366 / VP16 minimal transcriptio... 7:
                     2744 .. 2749 / #cc99b2
                     8: 2750 .. 3367 / #993366 / rTetR"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(3368..3421)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /note="color: #cc99b2
                     Cleavage site after base 3370"
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(3369..3392)
                     /label=1.54
                     /note="color: black; sequence: 
                     tgacgtcgaggagaatcctggcccaGTGAGCAAGGGCGAGGAGC; added: 
                     2020-11-26"
     misc_feature    complement(3638..3657)
                     /label=sgRNA ActB_FA
                     /note="color: #a6acb3; direction: LEFT
                     Cleavage site after base 3640"
     promoter        3662..4162
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     4167..4187
                     /label=pcDNA3_for
                     /note="color: black; sequence: GGCTAACTAGAGAACCCACTG;
                     added: 2021-10-18"
     primer_bind     4182..4224
                     /label=1.51
                     /note="color: black; sequence: 
                     ccactgcttactggcttatcgaaatataACCGTCAGATCCGCTAGCGC; added: 
                     2020-11-20"
     primer_bind     complement(4182..4209)
                     /label=MP1.6
                     /note="color: black; sequence: 
                     tccgcgcccgatggtgggacggtatatttcgataagccagtaagcagtgg; added: 
                     2020-09-25"
     primer_bind     4182..4208
                     /label=1.48
                     /note="color: black; sequence: 
                     CCACTGCTTACTGGCTTATCGAAATatgaccgagtacaagcccacg; added: 
                     2021-04-12"
     primer_bind     complement(4182..4206)
                     /label=1.7
                     /note="color: black; sequence: atttcgataagccagtaagcagtgg;
                     added: 2020-11-20"
     primer_bind     4201..4245
                     /label=3.83
                     /note="color: black; sequence: 
                     cgaaatataACCGTCAGATCCGCTGCCACCATGGTGAGCGTGATC; added: 
                     2023-10-20"
     primer_bind     complement(4201..4224)
                     /label=1.98
                     /note="color: black; sequence: AGCGGATCTGACGGTtatatttcg;
                     added: 2023-10-18"
     regulatory      4225..4234
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
     CDS             4231..4908
                     /codon_start=1
                     /product="humanized monomeric Azami-Green fluorescent
                     protein"
                     /label=hmAzami-Green
                     /note="mammalian codon-optimized"
                     /note="color: #05fd14"
                     /translation="MVSVIKPEMKIKLCMRGTVNGHNFVIEGEGKGNPYEGTQILDLNV
                     TEGAPLPFAYDILTTVFQYGNRAFTKYPADIQDYFKQTFPEGYHWERSMTYEDQGICTA
                     TSNISMRGDCFFYDIRFDGTNFPPNGPVMQKKTLKWEPSTEKMYVEDGVLKGDVNMRLL
                     LEGGGHYRCDFKTTYKAKKEVRLPDAHKIDHRIEILKHDKDYNKVKLYENAVARYSMLP
                     SQAK"
     primer_bind     complement(4890..4932)
                     /label=3.84
                     /note="color: black; sequence: 
                     ATCAGCTCGAGATCTGAGTCCGGACTTGGCCTGGCTGGGCAGC; added: 
                     2023-10-20"
     primer_bind     4909..4930
                     /label=3.85
                     /note="color: black; sequence: TCCGGACTCAGATCTCGAGCTG;
                     added: 2023-10-20"
     CDS             4933..4953
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     CDS             4957..4977
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     CDS             4981..5001
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     primer_bind     complement(4998..5011)
                     /label=1.52
                     /note="color: black; sequence: 
                     tatgtttcaggttcagggggaggtgTTATCTAGATCCGGTGGATCCTACC; added: 
                     2020-11-20"
     CDS             5011..5064
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /note="color: #cc99b2
                     Cleavage site after base 5061"
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     5040..5063
                     /label=1.54
                     /note="color: black; sequence: 
                     tgacgtcgaggagaatcctggcccaGTGAGCAAGGGCGAGGAGC; added: 
                     2020-11-26"
     CDS             5071..6096
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /note="color: #ccffcc"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(5273..5315)
                     /label=2.5
                     /note="color: black; sequence: 
                     tccccaatgtcaagcacttccggaatcgggagcgcggccgatg; added: 
                     2023-10-18"
     primer_bind     5291..5313
                     /label=2.6
                     /note="color: black; sequence: ttccggaagtgcttgacattggg;
                     added: 2023-10-18"
     primer_bind     6097..6118
                     /label=1.4
                     /note="color: black; sequence: cacctccccctgaacctgaaac;
                     added: 2021-04-12"
     polyA_signal    6144..6225
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(6225..6274)
                     /label=1.70
                     /note="color: black; sequence: 
                     cagagtgccagccctgggaccgaacTCTTCTAGGCTCAAGCAGTGATCAG; added: 
                     2021-02-15"
     primer_bind     complement(6250..6274)
                     /label=1.32
                     /note="color: black; sequence: 
                     cagagtgccagccctgggaccgaacCAAGCAGTGATCAGATCCAGACATG; added: 
                     2021-04-12"
     primer_bind     6250..6267
                     /label=1.23
                     /note="color: black; sequence: gttcggtcccagggctgg; added: 
                     2020-10-01"
     primer_bind     complement(6281..6304)
                     /label=1.67
                     /note="color: black; sequence: aaatctttagaccatagagcgttc;
                     added: 2021-04-12"
     misc_feature    6282..6304
                     /label=bigbac_alpha
                     /note="color: #ffff00"
ORIGIN
        1 aaatctatat ctcaatcggg gttacgtcga taccccaccg agaccccatt gggaccaata
       61 cgcccgcgtt tcttcctttt ccccacccca acccccaagt tcgggtgaag gcccagggct
      121 cgcagccaac gtcggggcgg caagccctgc catagccact acgggttaat taaaaactat
      181 atctcaatcg gggtttaaac aggagatccg gagttttcgt tccactgagc gtcagacccc
      241 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
      301 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
      361 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
      421 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
      481 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
      541 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
      601 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
      661 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
      721 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
      781 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
      841 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcccc gatctcggct
      901 tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac ttcttcccgt
      961 atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc ttgcacgtag
     1021 atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag cgcggtggca
     1081 atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat ctcactacgc
     1141 ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc caacaaccgc ttcttggtcg
     1201 aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta atcggagtcc
     1261 ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag atcaagagca
     1321 gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat gcccatactt
     1381 gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt gctgctgcgt
     1441 aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt
     1501 ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga aaaccgccac
     1561 tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg agcgcatacg
     1621 ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc gtgccttcgc
     1681 cgcggtacca taacttcgta tagcatacat tatacgaagt tatctgggtt taaacgctct
     1741 atggtctaaa gtttttatcg aggctaactg aaacacggaa gatccagaca tgataagata
     1801 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     1861 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     1921 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtcaggca ccgggcttgc
     1981 gggtcatgca ccaggtgcgc ggtccttcgg gcacctcgac gtcggcggtg acggtgaagc
     2041 cgagccgctc gtagaagggg aggttgcggg gcgcggaggt ctccaggaag gcgggcaccc
     2101 cggcgcgctc ggccgcctcc actccgggga gcacgacggc gctgcccaga cccttgccct
     2161 ggtggtcggg cgagactccg acggtggcca ggaaccacgc gggctccttg ggccggtgcg
     2221 gcgccaggag gccttccatc tgttgctgcg cggccagccg ggaaccgctc aactcggcca
     2281 tgcgcgggcc gatctcggcg aacaccgccc ccgcttcgac gctctccggc gtggtccaga
     2341 ccgccaccgc ggcgccgtcg tccgcgaccc acaccttgcc gatgtcgagc ccgacgcgcg
     2401 tgaggaagag ttcttgcagc tcggtgaccc gctcgatgtg gcggtccgga tcgacggtgt
     2461 ggcgcgtggc ggggtagtcg gcgaacgcgg cggcgagggt gcgtacggcc ctggggacgt
     2521 cgtcgcgggt ggcgaggcgc accgtgggct tgtactcggt catcccagga ccggggtttt
     2581 cttccacgtc tcctgcttgc tttaacagag agaagttcgt ggccccgggg agcatgtcaa
     2641 ggtcaaaatc gtcaagagcg tcagcaggca gcatatcaag gtcaaagtcg tcaagggcat
     2701 cggctgggag catgtctaag tcaaaatcgt caagggcgtc ggccggcccg ccgctttcgc
     2761 actttagctg tttctccagg ccacatatga ttagttccag gccgaaaagg aaggcaggtt
     2821 cggctccctg ccggtcgaac agctcaattg cttgtctcag aagtgggggc atagaatcgg
     2881 tggtaggtgt ctctctttcc tcttttgcta cttgatgctc ctgttcctcc aatacgcagc
     2941 ccagtgtaaa gtggcccacg gcggacagag cgtacagtgc gttctccagg gagaagcctt
     3001 gctgacacag gaacgcgagc tgattttcca gggtttcgta ctgtttctct gttgggcggg
     3061 tgccgagatg cactttagcc ccgtcgcgat gtgagaggag agcacagcgg aatgacttgg
     3121 cgttgttccg cagaaagtct tgccatgact cgccttccag ggggcagaag tgggtatgat
     3181 gcctgtccag catctcgatt ggcagggcat cgagcagggc ccgcttgttc ttcacgtgcc
     3241 agtacagggt aggctgctca actcccagct tttgagcgag tttccttgtc gtcaggcctt
     3301 cgataccgac tccattgagt aattccagag cgccgtttat gactttgctc ttgtccagtc
     3361 tagacatggg gccaggattc tcctcgacgt caccgcatgt tagcagactt cctctgccct
     3421 ctcccgatcc ggccccgaag catttgcggt ggacgatgga ggggccggac tcgtcatact
     3481 cctgcttgct gatccacatc tgctggaagg tggacagcga ggccaggatg gagccgccga
     3541 tccacacgga gtacttgcgc tcaggaggag caatgatctg aggagggaag gggacaggca
     3601 gtgaggaccc tggatgtgac agctccccac acaccctgtg gtgtgtgggg agctgtctca
     3661 tttgcgcctt ttccaaggca gccctgggtt tgcgcaggga cgcggctgct ctgggcgtgg
     3721 ttccgggaaa cgcagcggcg ccgaccctgg gtctcgcaca ttcttcacgt ccgttcgcag
     3781 cgtcacccgg atcttcgccg ctacccttgt gggccccccg gcgacgcttc ctgctccgcc
     3841 cctaagtcgg gaaggttcct tgcggttcgc ggcgtgccgg acgtgacaaa cggaagccgc
     3901 acgtctcact agtaccctcg cagacggaca gcgccaggga gcaatggcag cgcgccgacc
     3961 gcgatgggct gtggccaata gcggctgctc agcagggcgc gccgagagca gcggccggga
     4021 aggggcggtg cgggaggcgg ggtgtggggc ggtagtgtgg gccctgttcc tgcccgcgcg
     4081 gtgttccgca ttctgcaagc ctccggagcg cacgtcggca gtcggctccc tcgttgaccg
     4141 aatcaccgac ctctctcccc agctctggct aactagagaa cccactgctt actggcttat
     4201 cgaaatataa ccgtcagatc cgctgccacc atggtgagcg tgatcaagcc cgagatgaag
     4261 atcaagctgt gcatgagggg caccgtgaac ggccacaact tcgtgatcga gggcgagggc
     4321 aagggcaacc cctacgaggg cacccagatc ctggacctga acgtgaccga gggcgccccc
     4381 ctgcccttcg cctacgacat cctgaccacc gtgttccagt acggcaacag ggccttcacc
     4441 aagtaccccg ccgacatcca ggactacttc aagcagacct tccccgaggg ctaccactgg
     4501 gagaggagca tgacctacga ggaccagggc atctgcaccg ccaccagcaa catcagcatg
     4561 aggggcgact gcttcttcta cgacatcagg ttcgacggca ccaacttccc ccccaacggc
     4621 cccgtgatgc agaagaagac cctgaagtgg gagcccagca ccgagaagat gtacgtggag
     4681 gacggcgtgc tgaagggcga cgtgaacatg aggctgctgc tggagggcgg cggccactac
     4741 aggtgcgact tcaagaccac ctacaaggcc aagaaggagg tgaggctgcc cgacgcccac
     4801 aagatcgacc acaggatcga gatcctgaag cacgacaagg actacaacaa ggtgaagctg
     4861 tacgagaacg ccgtggccag gtactccatg ctgcccagcc aggccaagtc cggactcaga
     4921 tctcgagctg atccaaaaaa gaagagaaag gtagatccaa aaaagaagag aaaggtagat
     4981 ccaaaaaaga agagaaaggt aggatccacc gagggcagag gaagtctgct aacatgcggt
     5041 gacgtcgagg agaatcctgg ccccgtatac atgaaaaagc ctgaactcac cgcgacgtct
     5101 gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag
     5161 ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta
     5221 aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc
     5281 gcgctcccga ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc
     5341 atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct
     5401 gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg
     5461 agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc
     5521 atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc
     5581 agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa
     5641 gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc
     5701 ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc
     5761 aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag
     5821 cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt
     5881 cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag
     5941 ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc
     6001 cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac
     6061 cgacgcccca gcactcgtcc gagggcaaag gaatagcacc tccccctgaa cctgaaacaa
     6121 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     6181 gcaatagcat cacaaatttc acaaataaag catttttttc actgctgatc actgcttgag
     6241 cctagaagag ttcggtccca gggctggcac tctgtcaagt gaacgctcta tggtctaaag
     6301 attt
//
