LOCUS       pPLP1H-mCf              4916 bp    DNA     circular UNA 12-NOV-2024
DEFINITION  .
ACCESSION   urn.local...k8-ifxjivp
VERSION     urn.local...k8-ifxjivp
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     promoter        1..19
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /label="T7 promoter"
     RBS             33..55
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
                     /label="RBS"
     misc_feature    complement(42..61)
                     /created_by="User"
                     /label="pEN6 LIC tag - Vector R"
     misc_feature    43..65
                     /created_by="User"
                     /label="pEN6 LIC tag - Insert F"
     CDS             63..1244
                     /created_by="User"
                     /modified_by="User"
                     /label="Maturation Protein A"
     CDS             1268..2068
                     /created_by="User"
                     /modified_by="User"
                     /label="Single Chain Coat Protein Dimer "
     misc_feature    1700..1717
                     /created_by="User"
                     /modified_by="User"
                     /label="6xHis tag"
     terminator      2087..2139
                     /created_by="User"
                     /modified_by="User"
                     /label="Lambda T1 terminator"
     promoter        2147..2165
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     misc_feature    complement(2192..2212)
                     /created_by="User"
                     /modified_by="User"
                     /label="Ctrl LIC tag - vector R"
     misc_feature    2193..2213
                     /created_by="User"
                     /modified_by="User"
                     /label="Ctrl LIC tag - Insert F"
     misc_RNA        2213..2751
                     /created_by="User"
                     /modified_by="User"
                     /label="mCherry fragment"
     misc_feature    complement(2752..2773)
                     /created_by="User"
                     /modified_by="User"
                     /label="Ctrl LIC tag - Insert R"
     misc_feature    2759..2774
                     /created_by="User"
                     /modified_by="User"
                     /label="Ctrl LIC tag - Insert F"
     misc_RNA        2775..2793
                     /created_by="User"
                     /modified_by="User"
                     /label="TMpac"
     misc_feature    complement(2800..2819)
                     /created_by="User"
                     /label="pEN6 LIC tag - Insert R"
     misc_feature    2801..2820
                     /created_by="User"
                     /label="pEN6 LIC tag - Vector F"
     terminator      2827..2878
                     /created_by="User"
                     /label="T0 terminator"
     terminator      complement(2896..2929)
                     /created_by="User"
                     /modified_by="User"
                     /label="BBa_B1002 terminator"
     CDS             complement(2936..3796)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /label="AmpR"
     promoter        complement(3797..3901)
                     /gene="bla"
                     /label="AmpR promoter"
     terminator      complement(3917..3964)
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
                     /label="T7 terminator"
     rep_origin      4076..4621
                     /created_by="User"
                     /label="p15A ori"
ORIGIN      
        1 taatacgact cactataggg ataatccatc attttgttta actttaagaa ggagataaga
       61 acatgcgagc ttttagtacc cttgataggg agaacgagac cttcgtcccc tccgttcgcg
      121 tttacgcgga cggtgagact gaagataact cattctcttt aaaatatcgt tcgaactgga
      181 ctcccggtcg ttttaactcg actggggcca aaacgaaaca gtggcactac ccctctccgt
      241 attcacgggg ggcgttaagt gtcacatcga tagatcaagg tgcctacaag cgaagtgggt
      301 catcgtgggg tcgcccgtac gaggagaaag ccggtttcgg cttctccctc gacgcacgct
      361 cctgctacag cctcttccct gtaagccaga acttgactta catcgaagtg ccgcagaacg
      421 ttgcgaaccg ggcgtcgacc gaagtcctgc aaaaggtcac ccagggtaat tttaaccttg
      481 gtgttgcttt agcagaggcc aggtcgacag cctcacaact cgcgacgcaa accattgcgc
      541 tcgtgaaggc gtacactgcc gctcgtcgcg gtaattggcg ccaggcgctc cgctaccttg
      601 ccctaaacga agatcgaaag tttcgatcaa aacacgtggc cggcaggtgg ttggagttgc
      661 agttcggttg gttaccacta atgagtgata tccagggtgc ctatgagatg cttacgaagg
      721 ttcaccttca agagtttctt cctatgagag ccgtacgtca ggtcggtact aacatcaagt
      781 taaatggccg tctgtcgtat ccagctgcaa acttccagac aacgtgcaac atatcgcgac
      841 gtatcgtgat atggttttac ataaacgatg cacgtttggc atggttgtcg tctctaggta
      901 tcttgaaccc actaggtata gtgtgggaaa aggtgccttt ctcattcgtt gtcgactggc
      961 tcctacctgt aggtaacatg ctcgagggcc ttacggcccc cgtgggatgc tcctacatgt
     1021 caggaacagt tactgacgta ataacgggtg agtccatcat aagcgttgac gctccctacg
     1081 ggtggactgt ggagagacag ggcactgcta aggcccaaat ctcagccatg catcgagggg
     1141 tacaatccgt atggccaaca actggcgcgt acgtaaagtc tcctttctcg atggtccata
     1201 ccttagatgc gttagcatta atcaggcaac ggctctctag atagagccct caaccggagt
     1261 ttgaagcatg gcttctaact ttactcagtt cgttctcgtc gacaatggcg gaactggcga
     1321 cgtgactgtg gccccaagca acttcgctaa cggggtcgct gaatggatca gctctaactc
     1381 gcgttcacag gcttacaaag taacctgtag cgttcgtcag agctctgcgc agaatcgcaa
     1441 atacaccatc aaagtcgagg tgcctaaagt ggcaacccag actgttggtg gtgtagagct
     1501 tcctgtagcc gcatggcgtt cgtacttaaa tatggaacta accattccaa ttttcgctac
     1561 gaattccgac tgcgagctta ttgttaaggc aatgcaaggt ctcctaaaag atggaaaccc
     1621 gattccctca gcaatcgcag caaactccgg catctacgct aactttacgc aatttgtctt
     1681 ggtagataac ggcggtaccc atcaccatca tcaccacggc accggtgatg taaccgtagc
     1741 tccgtctaat tttgcaaatg gcgttgccga gtggatttcg agcaattccc gcagtcaagc
     1801 atataaggtg acgtgctccg tccggcaatc atcagctcaa aaccggaagt atacgattaa
     1861 ggttgaagtc ccgaaggtag ccactcaaac cgtagggggg gttgaattac cagttgcggc
     1921 gtggcgctca tatctcaaca tggagctgac gatcccgatc tttgcaacca actctgattg
     1981 tgaactgatc gtaaaagcta tgcagggcct gcttaaggac ggtaatccaa tcccatccgc
     2041 tattgcggcc aatagcggga tttactaata ataagcgcaa aaaaccctgt aacagagcat
     2101 tagcgcaagg tgatttttgt cttcttgcgc taatttttta gtgccgtaat acgactcact
     2161 atagggatct tagtatatta gttaagtata acaggataat aagtgtataa atccaaggag
     2221 ttcatgcggt ttaaggtcca tatggaaggg tctgtcaacg ggcatgaatt cgaaatcgaa
     2281 ggggagggcg agggtcgtcc gtacgaaggg acccaaacgg cgaagcttaa ggtaacgaag
     2341 ggtggtccac ttccgtttgc gtgggatatt cttagtccgc aatttatgta tggctccaaa
     2401 gcatacgtta aacaccctgc cgacattccg gactatctca aactctcgtt cccggaaggt
     2461 tttaagtggg agcgggtcat gaactttgag gacggtggcg tggtgactgt gacacaggat
     2521 tcatctttgc aagacgggga gttcatttac aaagtaaagt tgcgggggac gaacttcccg
     2581 tcggacggtc cggttatgca gaagaaaacc atggggtggg aagcctccag tgagcgtatg
     2641 taccctgaag atggtgctct taagggcgaa attaaacagc gccttaaatt gaaagatggc
     2701 ggtcattacg atgcagaggt aaagacaacg tataaggcga agaaaccagt ttgatgagat
     2761 ttccataacc aacgacatga ggatcaccca tgttaataag attatcatca actcatacag
     2821 agaggattgt tcagaacgct cggtcttgca caccgggcgt tttttctttg tgagtccaaa
     2881 ctcgagtggc gcgccgcgaa aaaaccccgc cgaagcgggg ttttttgcgt gacagttacc
     2941 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     3001 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     3061 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     3121 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     3181 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     3241 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     3301 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     3361 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     3421 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     3481 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     3541 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     3601 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     3661 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     3721 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     3781 aatgttgaat actcatactc ttcctttttc aatgttattg aagcatttat cagggttatt
     3841 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     3901 gcacatttgc ggccgccaaa aaacccctca agacccgttt agaggcccca aggggttatg
     3961 ctagtcattt atttaacaac ttatatcgta tggggctgac ttcaggtgct acatttgaag
     4021 agataaattg cactgaaatc tagaaatatt ttatctgatt aataagatga tcttcttgag
     4081 atcgttttgg tctgcgcgta atctcttgct ctgaaaacga aaaaaccgcc ttgcagggcg
     4141 gtttttcgaa ggttctctga gctaccaact ctttgaaccg aggtaactgg cttggaggag
     4201 cgcagtcacc aaaacttgtc ctttcagttt agccttaacc ggcgcatgac ttcaagacta
     4261 actcctctaa atcaattacc agtggctgct gccagtggtg cttttgcatg tctttccggg
     4321 ttggactcaa gacgatagtt accggataag gcgcagcggt cggactgaac ggggggttcg
     4381 tgcatacagt ccagcttgga gcgaactgcc tacccggaac tgagtgtcag gcgtggaatg
     4441 agacaaacgc ggccataaca gcggaatgac accggtaaac cgaaaggcag gaacaggaga
     4501 gcgcacgagg gagccgccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     4561 ccaccactga tttgagcgtc agatttcgtg atgcttgtca ggggggcgga gcctatggaa
     4621 aaacggcttt gccgcggccc tctcacttcc ctgttaagta tcttcctggc atcttccagg
     4681 aaatctccgc cccgttcgta agccatttcc gctcgccgca gtcgaacgac cgagcgtagc
     4741 gagtcagtga gcgaggaagc ggaatatatc ctgtatcaca tattctgctg acgcaccggt
     4801 gcagcctttt ttctcctgcc acatgaagca cttcactgac accctcatca gtgccaacat
     4861 agtaagccag tatacactcc gctagcgctg acctgcagga gcgatcgcag ggaatt
//
