LOCUS       pPLP3H-mCf              5017 bp    DNA     circular UNA 12-NOV-2024
DEFINITION  .
ACCESSION   urn.local...l0-ifxjs3c
VERSION     urn.local...l0-ifxjs3c
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     promoter        1..19
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /label="T7 promoter"
     RBS             33..55
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
                     /label="RBS"
     misc_feature    complement(42..61)
                     /created_by="User"
                     /label="pEN6 LIC tag - Vector R"
     misc_feature    43..65
                     /created_by="User"
                     /label="pEN6 LIC tag - Insert F "
     CDS             63..851
                     /created_by="User"
                     /modified_by="User"
                     /label="Single Chain Coat Protein Dimer "
     misc_feature    complement(470..487)
                     /created_by="User"
                     /modified_by="User"
                     /label="CPD LIC tag - Vector R"
     misc_feature    471..488
                     /created_by="User"
                     /modified_by="User"
                     /label="CPD LIC tag - Insert F"
     misc_feature    489..506
                     /created_by="User"
                     /modified_by="User"
                     /label="6xHis tag"
     misc_feature    complement(507..536)
                     /created_by="User"
                     /modified_by="User"
                     /label="CPD LIC tag - Insert R"
     misc_feature    523..537
                     /created_by="User"
                     /modified_by="User"
                     /label="CPD LIC tag - Vector F"
     terminator      860..901
                     /created_by="User"
                     /label="T22, P22 late terminator"
     promoter        909..927
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /label="T7 promoter"
     RBS             949..963
                     /created_by="User"
                     /modified_by="User"
                     /label="BBa_B0030 RBS"
     CDS             970..2151
                     /created_by="User"
                     /modified_by="User"
                     /label="Manturation Protein A"
     terminator      2168..2220
                     /created_by="User"
                     /modified_by="User"
                     /label="Lambda T1 terminator"
     promoter        2228..2246
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     promoter        2228..2246
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /label="T7 promoter"
     misc_feature    complement(2255..2275)
                     /created_by="User"
                     /modified_by="User"
                     /label="Ctrl LIC tag - Vector R"
     misc_feature    2256..2276
                     /created_by="User"
                     /modified_by="User"
                     /label="Ctrl LIC tag - Insert F"
     misc_RNA        2277..2814
                     /created_by="User"
                     /modified_by="User"
                     /label="mCherry fragment"
     misc_feature    complement(2815..2836)
                     /created_by="User"
                     /modified_by="User"
                     /label="Ctrl LIC tag - Insert R"
     misc_feature    2822..2837
                     /created_by="User"
                     /modified_by="User"
                     /label="Ctrl LIC tag - Vector F"
     misc_RNA        2838..2856
                     /created_by="User"
                     /modified_by="User"
                     /label="TMpac"
     misc_feature    2857..2875
                     /created_by="User"
                     /modified_by="User"
                     /label="pac spacer"
     misc_RNA        2876..2894
                     /created_by="User"
                     /modified_by="User"
                     /label="TMpac"
     misc_feature    complement(2901..2920)
                     /created_by="User"
                     /label="pEN6 LIC tag - Insert R"
     misc_feature    2902..2921
                     /created_by="User"
                     /modified_by="User"
                     /label="pEN6 LIC tag - Vector F"
     terminator      2928..2979
                     /created_by="User"
                     /label="T0 terminator"
     terminator      complement(2997..3030)
                     /created_by="User"
                     /modified_by="User"
                     /label="BBa_B1002 terminator"
     gene            complement(3031..4009)
                     /created_by="User"
                     /label="AmpR cassette"
     CDS             order(complement(<3190..3897),complement(3037..>3189))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /label="AmpR"
     promoter        complement(3898..4002)
                     /gene="bla"
                     /label="AmpR promoter"
     terminator      complement(4018..4065)
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
                     /label="T7 terminator"
     rep_origin      4177..4722
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
                     /label="p15A ori"
ORIGIN      
        1 taatacgact cactataggg ataatccatc attttgttta actttaagaa ggagataaga
       61 acatggcttc taactttact cagttcgttc tcgtcgacaa tggcggaact ggcgacgtga
      121 ctgtggcccc aagcaacttc gctaacgggg tcgctgaatg gatcagctct aactcgcgtt
      181 cacaggctta caaagtaacc tgtagcgttc gtcagagctc tgcgcagaat cgcaaataca
      241 ccatcaaagt cgaggtgcct aaagtggcaa cccagactgt tggtggtgta gagcttcctg
      301 tagccgcatg gcgttcgtac ttaaatatgg aactaaccat tccaattttc gctacgaatt
      361 ccgactgcga gcttattgtt aaggcaatgc aaggtctcct aaaagatgga aacccgattc
      421 cctcagcaat cgcagcaaac tccggcatct acgctaactt tacgcaattc gtgttggttg
      481 ataatggcca tcaccatcat caccacggtg atgtaaccgt agctccatct aacttcgcaa
      541 atggcgttgc cgagtggatt tcgagcaatt cccgcagtca agcatataag gtgacgtgct
      601 ccgtccggca atcatcagct caaaaccgga agtatacgat taaggttgaa gtcccgaagg
      661 tagccactca aaccgtaggc ggtgttgaat taccagttgc ggcgtggcgc tcatatctca
      721 acatggagct gacgatcccg atctttgcaa ccaactctga ttgtgaactg atcgtaaaag
      781 ctatgcaggg cctgcttaag gacggtaatc caatcccatc cgctattgcg gccaatagcg
      841 ggatttacta ataaaaacca aataaagccc tgagtttaac cgctcggggc tttttgcgtt
      901 tagtgccgta atacgactca ctatagggaa atacaacctt cttgagtcat taaagaggag
      961 aaaatacata tgcgagcttt tagtaccctt gatagggaga acgagacctt cgtcccctcc
     1021 gttcgcgttt acgcggacgg tgagactgaa gataactcat tctctttaaa atatcgttcg
     1081 aactggactc ccggtcgttt taactcgact ggggccaaaa cgaaacagtg gcactacccc
     1141 tctccgtatt cacggggggc gttaagtgtc acatcgatag atcaaggtgc ctacaagcga
     1201 agtgggtcat cgtggggtcg cccgtacgag gagaaagccg gtttcggctt ctccctcgac
     1261 gcacgctcct gctacagcct cttccctgta agccagaact tgacttacat cgaagtgccg
     1321 cagaacgttg cgaaccgggc gtcgaccgaa gtcctgcaaa aggtcaccca gggtaatttt
     1381 aaccttggtg ttgctttagc agaggccagg tcgacagcct cacaactcgc gacgcaaacc
     1441 attgcgctcg tgaaggcgta cactgccgct cgtcgcggta attggcgcca ggcgctccgc
     1501 taccttgccc taaacgaaga tcgaaagttt cgatcaaaac acgtggccgg caggtggttg
     1561 gagttgcagt tcggttggtt accactaatg agtgatatcc agggtgccta tgagatgctt
     1621 acgaaggttc accttcaaga gtttcttcct atgagagccg tacgtcaggt cggtactaac
     1681 atcaagttaa atggccgtct gtcgtatcca gctgcaaact tccagacaac gtgcaacata
     1741 tcgcgacgta tcgtgatatg gttttacata aacgatgcac gtttggcatg gttgtcgtct
     1801 ctaggtatct tgaacccact aggtatagtg tgggaaaagg tgcctttctc attcgttgtc
     1861 gactggctcc tacctgtagg taacatgctc gagggcctta cggcccccgt gggatgctcc
     1921 tacatgtcag gaacagttac tgacgtaata acgggtgagt ccatcataag cgttgacgct
     1981 ccctacgggt ggactgtgga gagacagggc actgctaagg cccaaatctc agccatgcat
     2041 cgaggggtac aatccgtatg gccaacaact ggcgcgtacg taaagtctcc tttctcgatg
     2101 gtccatacct tagatgcgtt agcattaatc aggcaacggc tctctagata atagatccaa
     2161 ctccatactg taacagagca ttagcgcaag gtgatttttg tcttcttgcg ctaatttttt
     2221 agtgccgtaa tacgactcac tatagggagg tcgacaggat aataagtgta taaatccaag
     2281 gagttcatgc ggtttaaggt ccatatggaa gggtctgtca acgggcatga attcgaaatc
     2341 gaaggggagg gcgagggtcg tccgtacgaa gggacccaaa cggcgaagct taaggtaacg
     2401 aagggtggtc cacttccgtt tgcgtgggat attcttagtc cgcaatttat gtatggctcc
     2461 aaagcatacg ttaaacaccc tgccgacatt ccggactatc tcaaactctc gttcccggaa
     2521 ggttttaagt gggagcgggt catgaacttt gaggacggtg gcgtggtgac tgtgacacag
     2581 gattcatctt tgcaagacgg ggagttcatt tacaaagtaa agttgcgggg gacgaacttc
     2641 ccgtcggacg gtccggttat gcagaagaaa accatggggt gggaagcctc cagtgagcgt
     2701 atgtaccctg aagatggtgc tcttaagggc gaaattaaac agcgccttaa attgaaagat
     2761 ggcggtcatt acgatgcaga ggtaaagaca acgtataagg cgaagaaacc agtttgatga
     2821 gatttccata accaacgaca tgaggatcac ccatgtctgc aggtcgactc tagaaacatg
     2881 aggatcaccc atgttaataa gattatcatc aactcataca gagaggattg ttcagaacgc
     2941 tcggtcttgc acaccgggcg ttttttcttt gtgagtccaa actcgagtgg cgcgccgcga
     3001 aaaaaccccg ccgaagcggg gttttttgcg tgacagttac caatgcttaa tcagtgaggc
     3061 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     3121 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     3181 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     3241 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     3301 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     3361 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     3421 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     3481 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     3541 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     3601 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     3661 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     3721 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     3781 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     3841 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     3901 cttccttttt caatgttatt gaagcattta tcagggttat tgtctcatga gcggatacat
     3961 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttg cggccgccaa
     4021 aaaacccctc aagacccgtt tagaggcccc aaggggttat gctagtcatt tatttaacaa
     4081 cttatatcgt atggggctga cttcaggtgc tacatttgaa gagataaatt gcactgaaat
     4141 ctagaaatat tttatctgat taataagatg atcttcttga gatcgttttg gtctgcgcgt
     4201 aatctcttgc tctgaaaacg aaaaaaccgc cttgcagggc ggtttttcga aggttctctg
     4261 agctaccaac tctttgaacc gaggtaactg gcttggagga gcgcagtcac caaaacttgt
     4321 cctttcagtt tagccttaac cggcgcatga cttcaagact aactcctcta aatcaattac
     4381 cagtggctgc tgccagtggt gcttttgcat gtctttccgg gttggactca agacgatagt
     4441 taccggataa ggcgcagcgg tcggactgaa cggggggttc gtgcatacag tccagcttgg
     4501 agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagacaaacg cggccataac
     4561 agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccgcca
     4621 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccaccactg atttgagcgt
     4681 cagatttcgt gatgcttgtc aggggggcgg agcctatgga aaaacggctt tgccgcggcc
     4741 ctctcacttc cctgttaagt atcttcctgg catcttccag gaaatctccg ccccgttcgt
     4801 aagccatttc cgctcgccgc agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag
     4861 cggaatatat cctgtatcac atattctgct gacgcaccgg tgcagccttt tttctcctgc
     4921 cacatgaagc acttcactga caccctcatc agtgccaaca tagtaagcca gtatacactc
     4981 cgctagcgct gacctgcagg agcgatcgca gggaatt
//
