LOCUS       pAAV-UAS-mIFNγ        5088 bp DNA     circular SYN 19-JUN-2024
DEFINITION  SPARK reporter gene.
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-UAS-Luciferase
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5088)
  AUTHORS   Kim MW, Wang W, Sanchez MI, Coukos R, von Zastrow M, Ting AY
  TITLE     Time-gated detection of protein-protein interactions with 
            transcriptional readout.
  JOURNAL   Elife. 2017 Nov 30;6. doi: 10.7554/eLife.30233.
  PUBMED    29189201
REFERENCE   2  (bases 1 to 5088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 3, 2024 from SnapGene Viewer 6.2.0
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife. 2017
            Nov 30;6. doi: 10.7554/eLife.30233."
FEATURES             Location/Qualifiers
     source          1..5088
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
                     /note="color: #ffcc99"
     promoter        145..674
                     /label=UAS Promoter
                     /note="color: #ff99cc; direction: RIGHT"
     5'UTR           619..661
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     630..653
                     /label=NAK-XenGlob 5' UTR-F
                     /note="color: black; sequence: tgcagaagctcagaataaacgctc; 
                     added: 2019-08-16"
     regulatory      720..728
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
                     /regulatory_class="other"
     CDS             726..1190
                     /codon_start=1
                     /product="Human codon-optimized"
                     /label=Mouse IFNg
                     /note="color: #33cccc"
                     /translation="MNATHCILALQLFLMAVSGCYCHGTVIESLESLNNYFNSSGIDVE
                     EKSLFLDIWRNWQKDGDMKILQSQIISFYLRLFEVLKDNQAISNNISVIESHLITTFFS
                     NSKAKKDAFMSIAKFEVNNPQVQRQAFNELIRVVHQLLPESSLRKRKRSRC"
     misc_feature    1197..1231
                     /label=Sequence Validated (NAK-P1; Order 600014)
                     /note="color: #99ccff"
     misc_feature    1215..1803
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     primer_bind     complement(1268..1288)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
                     /note="color: black; sequence: CATAGCGTAAAAGGAGCAACA"
     CDS             complement(1686..1697)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 1685"
                     /translation="IEGR"
     polyA_signal    1835..2311
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(2034..2053)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
                     /note="color: black; sequence: CCAGCTTGGTTCCCAATAGA"
     repeat_region   2351..2491
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     repeat_region   2351..2480
                     /label=AAV2 ITR
                     /note="color: #ffcc99"
     rep_origin      2566..3021
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     primer_bind     complement(2653..2672)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
                     /note="color: black; sequence: AGGGAAGAAAGCGAAAGGAG"
     primer_bind     2863..2884
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
                     /note="color: black; sequence: GTGGACTCTTGTTCCAAACTGG"
     primer_bind     complement(3038..3057)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
                     /note="color: black; sequence: CGGCATCAGAGCAGATTGTA"
     primer_bind     3157..3179
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
                     /note="color: black; sequence: CCGGGAGCTGCATGTGTCAGAGG"
     primer_bind     complement(3217..3235)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
                     /note="color: black; sequence: AATAGGCGTATCACGAGGC"
     promoter        3303..3407
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             3408..4268
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 3408 .. 3476 / #ccffcc / signal sequence
                        2: 3477 .. 4268 / #ccffcc
                     Cleavage site after base 3476"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3626..3645)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
                     /note="color: black; sequence: ATAATACCGCGCCACATAGC"
     rep_origin      4439..5027
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     primer_bind     4928..4947
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
                     /note="color: black; sequence: GGGAAACGCCTGGTATCTTT"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgcaaaa agcttaggcc tccaaggcgg agtactgtcc
      181 tccgggctgg cggagtactg tcctccggca aggtcggagt actgtcctcc gacactagag
      241 gtcggagtac tgtcctccga cgcaaggcgg agtactgtcc tccgggctgc ggagtactgt
      301 cctccggcaa ggtcggagta ctgtcctccg acactagagg tcggagtact gtcctccgac
      361 gcaaggtcgg agtactgtcc tccgacacta gaggtcggag tactgtcctc cgacgcaagg
      421 tcggagtact gtcctccgac actagaggtc ggagtactgt cctccgacgc aaggcggagt
      481 actgtcctcc gggctggcgg agtactgtcc tccggcaagg gtcgactcta gagggtatat
      541 aatggatccc atcgcgtctc agcctcactt tgagctcctc cacacgaatt ccccgatacc
      601 gtcgattcaa ggagcttgct tgttcttttt gcagaagctc agaataaacg ctcaactttg
      661 gcagatctac caagcttggt accgagctcg gatccactag tgatccccgg gtaccggtcg
      721 ccaccatgaa cgccacgcac tgcatcctgg ctctccaact ctttcttatg gcggtcagtg
      781 gctgctactg tcacggtacc gtaatcgaga gcctggagtc cctcaataat tattttaatt
      841 ccagtgggat tgatgtagaa gaaaagagtc tctttctgga catctggaga aactggcaga
      901 aagacggcga catgaagatc ctccagtccc aaataatatc attttacttg cgactgtttg
      961 aggtcttgaa ggataatcag gccatctcaa acaatattag tgtaatcgaa tctcacctca
     1021 ttaccacttt tttttctaat tccaaggcga aaaaggacgc ttttatgtca atagctaaat
     1081 tcgaggtgaa taatcctcag gtacaacggc aggctttcaa tgaactgata cgcgtcgtcc
     1141 atcaattgct cccggagagc agcctccgga agaggaagcg cagccggtgc tgataggata
     1201 tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     1261 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     1321 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     1381 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     1441 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     1501 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     1561 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     1621 ttccttggct gctcgcctat gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     1681 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     1741 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     1801 cgcatcgata ccgagcgctg ctcgagagat ctacgggtgg catccctgtg acccctcccc
     1861 agtgcctctc ctggccctgg aagttgccac tccagtgccc accagccttg tcctaataaa
     1921 attaagttgc atcattttgt ctgactaggt gtccttctat aatattatgg ggtggagggg
     1981 ggtggtatgg agcaaggggc aagttgggaa gacaacctgt agggcctgcg gggtctattg
     2041 ggaaccaagc tggagtgcag tggcacaatc ttggctcact gcaatctccg cctcctgggt
     2101 tcaagcgatt ctcctgcctc agcctcccga gttgttggga ttccaggcat gcatgaccag
     2161 gctcagctaa tttttgtttt tttggtagag acggggtttc accatattgg ccaggctggt
     2221 ctccaactcc taatctcagg tgatctaccc accttggcct cccaaattgc tgggattaca
     2281 ggcgtgaacc actgctccct tccctgtcct tctgattttg taggtaacca cgtgcggacc
     2341 gagcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg
     2401 ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca
     2461 gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt tctccttacg
     2521 catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg
     2581 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     2641 ccttagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     2701 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     2761 tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga
     2821 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     2881 ctggaacaac actcaactct atctcgggct attcttttga tttataaggg attttgccga
     2941 tttcggtcta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     3001 aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat
     3061 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     3121 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     3181 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     3241 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     3301 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     3361 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     3421 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     3481 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     3541 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     3601 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     3661 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     3721 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     3781 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     3841 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     3901 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     3961 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     4021 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     4081 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     4141 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     4201 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     4261 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     4321 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     4381 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     4441 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     4501 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     4561 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     4621 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     4681 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     4741 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     4801 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     4861 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     4921 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     4981 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     5041 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgt
//
