LOCUS       pCLYBL-Neo_TRE3V        9237 bp    DNA     circular SYN 24-SEP-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 9237)
  AUTHORS   Bertero
  TITLE     Direct Submission
  JOURNAL   Exported Nov 14, 2024 from SnapGene 8.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9237
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     CDS             114..923
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /note="color: #ccffcc"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      1101..1689
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     protein_bind    1977..1998
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     promoter        join(2013..2018,2019..2036,2037..2043)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This forward directional feature has 3 segments:
                        1: 2013 .. 2018 / #ffffff / -35
                        2: 2019 .. 2036 / #ffffff
                        3: 2037 .. 2043 / #ffffff / -10"
     protein_bind    2051..2067
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     2075..2091
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     misc_feature    2111..2943
                     /label=CYLBL_HA-L
                     /note="color: #ff00ff"
     protein_bind    2952..2985
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
                     /note="color: #31849b; direction: RIGHT"
     misc_feature    2986..3011
                     /label=SA
                     /note="splice acceptor site"
                     /note="color: #a6acb3"
     CDS             3035..3088
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /note="color: #cc99b2
                     Cleavage site after base 3086"
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3092..3883
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /note="color: #ccffcc"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    3918..4142
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     protein_bind    4143..4176
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
                     /note="color: #31849b; direction: RIGHT"
     misc_feature    4202..5386
                     /label=Gallus gallus HS4 insulator
                     /note="color: #000000"
     primer_bind     5375..5415
                     /label=oMG014
                     /note="color: black; sequence: 
                     cagtgagaatattggcgcgccGGTGGGCTCTATGGTCACTC; added: 
                     2024-02-19"
     polyA_signal    5395..5409
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     promoter        5416..5765
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
                     /note="color: #ffffff; direction: RIGHT"
     protein_bind    5424..5442
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    5460..5478
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    5496..5514
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    5532..5550
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    5568..5586
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    5604..5622
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    5640..5658
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     primer_bind     5757..5793
                     /label=oMG010
                     /note="color: black; sequence: 
                     CTTATACTTACTAGTGCCACCatggtgagcaagggcg; added: 2023-12-11"
     misc_feature    5772..5781
                     /label=KOZAK SEQUENCE
                     /note="color: #a6acb3"
     CDS             5778..6488
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /note="color: #ff0000"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     6259..6278
                     /label=oEC158
                     /note="color: black; sequence: CCCTGAAGGGCGAGATCAAG; added:
                     2020-01-03"
     primer_bind     complement(6464..6508)
                     /label=oMG001
                     /note="color: black; sequence: 
                     CGGGTACCGAGCTCGAATTCttacttgtacagctcgtccatgccg; added: 
                     2023-12-11"
     primer_bind     complement(6465..6488)
                     /label=oEC134
                     /note="color: black; sequence: 
                     tgaggagtgaattcacgcgtttacttgtacagctcgtccatgcc; added: 
                     2020-01-03"
     primer_bind     6489..6506
                     /label=OSB023
                     /note="color: black; sequence: GAATTCGAGCTCGGTACC; added: 
                     2023-10-26"
     polyA_signal    6685..6766
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(6761..6807)
                     /label=oMG015
                     /note="color: black; sequence: 
                     gttaacttaattaaggtaccCGACTTAAGAGTACTGTCAAGGCAGTG; added: 
                     2024-02-19"
     misc_feature    6845..8029
                     /label=Gallus Gallus HS4 insulator
                     /note="color: #000000"
     misc_feature    8039..8836
                     /label=CYLBL_HA-R
                     /note="color: #ff00ff"
     primer_bind     complement(8843..8859)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
ORIGIN
        1 tgcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat
       61 catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc
      121 atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct gatttatatg
      181 ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg
      241 ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg
      301 ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca
      361 agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggaaaaa
      421 cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg
      481 cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc
      541 gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg
      601 attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac
      661 ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta
      721 tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc
      781 gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga
      841 aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt
      901 tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacat tattcagatt
      961 gggcttgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     1021 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     1081 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     1141 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     1201 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     1261 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     1321 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     1381 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     1441 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     1501 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     1561 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     1621 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     1681 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     1741 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     1801 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     1861 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     1921 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     1981 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     2041 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     2101 gccgcggccg cagcaggaga atcatgccaa tgggccaata tacattctga cccacagttt
     2161 cataataaaa taaaatggtt gtggttgtaa gccactatgt ttcagagtgg tttgttacac
     2221 agcaataaat aactaatata gtaggcatac catcaagtcc aaagtaggta gagaagaatg
     2281 taaatagcag agcaaaacag catgactggt ggctgggagg cttaaaactg ggacaggatc
     2341 agagtcatga aagaagtcaa agaaatggtt cagaagtaag gctgagactg acttacaaaa
     2401 gctgaaagtc cctttaagtt ggtgtttggt gcattggcag gggcaggtat ggtgacttaa
     2461 aagagccatg ctcaacaaga tcaagcacaa cacaatcacg ggtcacccca gcagacctta
     2521 gcgagtctag ccatttcttt ggtggtggtc acagtcatgc ttcagcccag tttccacttg
     2581 gacaaatggt acatattttc aatgagatga aaattaagat acaatccatg tgctcagaga
     2641 gtgatcacag ctctgactaa acactgtgcc ccaaagtgtt gaggaattgg gaaaacctag
     2701 ctgagttagt ggtctctttt ctgttacaat aaagctcata atgaaaatta gccttctttg
     2761 ttcttcccca agtctttctt tctagacgaa actactttca actgttttaa cttccttact
     2821 gttaacttcc atatttctag atagtatggt cagactgtta tttcttgact tttaaatgta
     2881 agatattatc tactgacttc cttctatgta agatgaggat tgagctctct tacccttctc
     2941 ccagcggccg cataacttcg tatagcatac attatacgaa gttatctgac ctcttctctt
     3001 cctcccacag ggcctcgaga gatctggcag cggagagggc agaggaagtc ttctaacatg
     3061 cggtgacgtg gaggagaatc ccggccctag gattgaacaa gatggattgc acgcaggttc
     3121 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     3181 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     3241 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc
     3301 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     3361 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     3421 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     3481 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     3541 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     3601 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     3661 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     3721 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     3781 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     3841 gcagcgcatc gccttctatc gccttcttga cgagttcttc taacacctgg tgcatgaccc
     3901 gcaagcccgg tgcctgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     3961 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     4021 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     4081 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     4141 ggataacttc gtatagcata cattatacga agttatgagc tcacggggac agcccccccc
     4201 caaagccccc agggatgtaa ttacgtccct cccccgctag ggggcagcag cgagccgccc
     4261 ggggctccgc tccggtccgg cgctcccccc gcatccccga gccggcagcg tgcggggaca
     4321 gcccgggcac ggggaaggtg gcacgggatc gctttcctct gaacgcttct cgctgctctt
     4381 tgagcctgca gacacctggg gggatacggg gaaaaagctt taggctgaaa gagagattta
     4441 gaatgacaga atcatagaac ggcctgggtt gcaaaggagc acagtgctca tccagatcca
     4501 accccctgct atgtgcaggg tcatcaacca gcagcccagg ctgcccagag ccacatccag
     4561 cctggccttg aatgcctgca gggatggggc atccacagcc tccttgggca acctgttcag
     4621 tgcgtcacca ccctctgggg gaaaaactgc ctcctcatat ccaacccaaa cctcccctgt
     4681 ctcagtgtaa agccattccc ccttgtccta tcaaggggga gtttgctgtg acattgttgg
     4741 tctggggtga cacatgtttg ccaattcagt gcatcacgga gaggcagatc ttggggataa
     4801 ggaagtgcag gacagcatgg acgtgggaca tgcaggtgtt gagggctctg ggacactctc
     4861 caagtcacag cgttcagaac agccttaagg ataagaagat aggatagaag gacaaagagc
     4921 aagttaaaac ccagcatgga gaggagcaca aaaaggccac agacactgct ggtccctgtg
     4981 tctgagcctg catgtttgat ggtgtctgga tgcaagcaga aggggtggaa gagcttgcct
     5041 ggagagatac agctgggtca gtaggactgg gacaggcagc tggagaattg ccatgtagat
     5101 gttcatacaa tcgtcaaatc atgaaggctg gaaaagccct ccaagatccc caagaccaac
     5161 cccaacccac ccaccgtgcc cactggccat gtccctcagt gccacatccc cacagttctt
     5221 catcacctcc agggacggtg acccccccac ctccgtgggc agctgtgcca ctgcagcacc
     5281 gctctttgga gaaggtaaat cttgctaaat ccagcccgac cctcccctgg cacaacgtaa
     5341 ggccattatc tctcatccaa ctccaggacg gagtcagtga gaatattggc gcgccggtgg
     5401 gctctatggt cactcgagtt tactccctat cagtgataga gaacgtatga agagtttact
     5461 ccctatcagt gatagagaac gtatgcagac tttactccct atcagtgata gagaacgtat
     5521 aaggagttta ctccctatca gtgatagaga acgtatgacc agtttactcc ctatcagtga
     5581 tagagaacgt atctacagtt tactccctat cagtgataga gaacgtatat ccagtttact
     5641 ccctatcagt gatagagaac gtatgtcgag gtaggcgtgt acggtgggcg cctataaaag
     5701 cagagctcgt ttagtgaacc gtcagatcgc ctggagcaat tccacaacac ttttgtctta
     5761 tacttactag tgccaccatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg
     5821 agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg
     5881 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
     5941 agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca
     6001 aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg
     6061 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg
     6121 actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc
     6181 cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga
     6241 tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg
     6301 gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc
     6361 ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca
     6421 tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt
     6481 acaagtaaga attcgagctc ggtacccggg gatcctctag tcagctgacg cgtgctagcg
     6541 cggccgcatc gataagcttg tcgacgatat ctctagagga tcataatcag ccataccaca
     6601 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     6661 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     6721 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgccttg acagtactct
     6781 taagtcgggt accttaatta agttaacatg catagtactg agctcacggg gacagccccc
     6841 ccccaaagcc cccagggatg taattacgtc cctcccccgc tagggggcag cagcgagccg
     6901 cccggggctc cgctccggtc cggcgctccc cccgcatccc cgagccggca gcgtgcgggg
     6961 acagcccggg cacggggaag gtggcacggg atcgctttcc tctgaacgct tctcgctgct
     7021 ctttgagcct gcagacacct ggggggatac ggggaaaaag ctttaggctg aaagagagat
     7081 ttagaatgac agaatcatag aacggcctgg gttgcaaagg agcacagtgc tcatccagat
     7141 ccaaccccct gctatgtgca gggtcatcaa ccagcagccc aggctgccca gagccacatc
     7201 cagcctggcc ttgaatgcct gcagggatgg ggcatccaca gcctccttgg gcaacctgtt
     7261 cagtgcgtca ccaccctctg ggggaaaaac tgcctcctca tatccaaccc aaacctcccc
     7321 tgtctcagtg taaagccatt cccccttgtc ctatcaaggg ggagtttgct gtgacattgt
     7381 tggtctgggg tgacacatgt ttgccaattc agtgcatcac ggagaggcag atcttgggga
     7441 taaggaagtg caggacagca tggacgtggg acatgcaggt gttgagggct ctgggacact
     7501 ctccaagtca cagcgttcag aacagcctta aggataagaa gataggatag aaggacaaag
     7561 agcaagttaa aacccagcat ggagaggagc acaaaaaggc cacagacact gctggtccct
     7621 gtgtctgagc ctgcatgttt gatggtgtct ggatgcaagc agaaggggtg gaagagcttg
     7681 cctggagaga tacagctggg tcagtaggac tgggacaggc agctggagaa ttgccatgta
     7741 gatgttcata caatcgtcaa atcatgaagg ctggaaaagc cctccaagat ccccaagacc
     7801 aaccccaacc cacccaccgt gcccactggc catgtccctc agtgccacat ccccacagtt
     7861 cttcatcacc tccagggacg gtgacccccc cacctccgtg ggcagctgtg ccactgcagc
     7921 accgctcttt ggagaaggta aatcttgcta aatccagccc gaccctcccc tggcacaacg
     7981 taaggccatt atctctcatc caactccagg acggagtcag tgagaatatt gtttaaactc
     8041 ctcatccttc caacataaat atattttggg attatatcaa cattcaatgt tacttaaagt
     8101 gaccttgtaa atattttcac aactgagcca tgtttgattt gtatacttat gtttacttta
     8161 ctgtttttcc tgaagttaat aattgccttg aatttattta tttctttaaa aatgtttcat
     8221 tactcaggac tgtagtttac attacgattc tttgtgttat acagttgatg ggtttctttt
     8281 ctttcttaat ttctttaaaa aatagagatg gggtcttact atattaccca ggctggtctt
     8341 gaagtcctgg gctcaagtga tcttcctgtc tcagcctacc aagtagctga gactataggt
     8401 gcaaaaaagc cactatacct ggctagttta caggttttaa caaatgcatt atgccacgta
     8461 tccattatta caggatcaca caagatattt tcattaccct gaaagcatcc ctgtgttcca
     8521 ccaattcatc ctgcctccat gagccgctgg caaccactga tctctatagt tttgcctttt
     8581 ctaaaatgtc atataattgg aatcatacag tctgtagcat tttcagacta gcttttaaaa
     8641 tttggcaata tgcatttaag gttcctcctt aaatgtgaag ggcatagcca atgtggcttg
     8701 atagctcatt tctttttatt ggtgaatatt tcattgtctg gatgttccac agtttgttta
     8761 tccattcacc tattcaattt gctttttttc tgtgtatcta tcactaattc aatactggac
     8821 tctccaacag agccgtttaa acactggccg tcgttttaca acgtcgtgac tgggaaaacc
     8881 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     8941 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc
     9001 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca
     9061 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
     9121 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     9181 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcga
//
