LOCUS       pMS05_(plasmidsaurus_ver_04/25/2025;_clone_A)        5856 bp    DNA     circular SYN 13-MAY-2025
DEFINITION  pMS05a.
ACCESSION   pMS05a
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     Annotated with pLannotate v1.2.2.
FEATURES             Location/Qualifiers
     rep_origin      1..389
     CDS             complement(419..1042)
                     /note="pLannotate"
                     /standard_name="TetR"
     misc_feature    1313..1331
                     /standard_name="TetO"
     misc_feature    1375..1393
                     /standard_name="TetO"
     misc_feature    1423..1564
                     /standard_name="MCS B"
     misc_feature    1566..1634
                     /note="Geneious type: motif"
                     /standard_name="3xFLAG"
     CDS             1939..2736
                     /note="pLannotate"
                     /standard_name="ermF"
     CDS             2865..3599
                     /standard_name="repA"
     CDS             4586..5446
                     /note="pLannotate"
                     /standard_name="AmpR"
     oriT            complement(5577..5686)
                     /note="pLannotate"
                     /standard_name="IncP oriT"
     oriT            5622..5720
                     /standard_name="RP4 oriT"
ORIGIN      
        1 gatctgaaga tcagcagttc aacctgttga tagtacgtac taagctctca tgtttcacgt
       61 actaagctct catgtttaac gtactaagct ctcatgttta acgaactaaa ccctcatggc
      121 taacgtacta agctctcatg gctaacgtac taagctctca tgtttcacgt actaagctct
      181 catgtttgaa caataaaatt aatataaatc agcaacttaa atagcctcta aggttttaag
      241 ttttataaga aaaaaaagaa tatataaggc ttttaaagct tttaaggttt aacggttgtg
      301 gacaacaagc cagggatgta acgcactgag aagcccttag agcctctcaa agcaattttg
      361 agtgacacag gaacacttaa cggctgacat gggaattctt cccctccacc gcggtggctt
      421 aagacccact ttcacattta agttgttttt ctaatccgca tatgatcaat tcaaggccga
      481 ataagaaggc tggctctgca ccttggtgat caaataattc gatagcttgt cgtaataatg
      541 gcggcatact atcagtagta ggtgtttccc tttcttcttt agcgacttga tgctcttgat
      601 cttccaatac gcaacctaaa gtaaaatgcc ccacagcgct gagtgcatat aatgcattct
      661 ctagtgaaaa accttgttgg cataaaaagg ctaattgatt ttcgagagtt tcatactgtt
      721 tttctgtagg ccgtgtacct aaatgtactt ttgctccatc gcgatgactt agtaaagcac
      781 atctaaaact tttagcgtta ttacgtaaaa aatcttgcca gctttcccct tctaaagggc
      841 aaaagtgagt atggtgccta tctaacatct caatggctaa ggcgtcgagc aaagcccgct
      901 tattttttac atgccaatac aatgtaggct gctctacacc tagcttctgg gcgagtttac
      961 gggttgttaa accttcgatt ccgacctcat taagcagctc taatgcgctg ttaatcactt
     1021 tacttttatc taatctagac atattcgttt aatatcataa ataatttatt ttattttaaa
     1081 atgcgcgggt gcaaaggtaa gaggttttat tttaactacc aaatgttttc ggaagttttt
     1141 tcgcttttct ttttctatcg tttctcagac tctcttagcg aaagggaaag aaggtaaaga
     1201 agaaaaacaa aacgcctttt ctttttttgc acccgctttc caagagaaga aagccttgtt
     1261 aaattgactt agtgtaaaag cgcagtactg cttgaccata agaacaaaaa aatctctatc
     1321 actgataggg ataaagtttg gaagataaag ctaaaagttc ttatctttgc agtctcccta
     1381 tcagtgatag agacgaaata aagacatata aaagaaaaga caccccgggc atgccaggat
     1441 ccgaattcga gctcggcgcg cctgcaggtc gacaagcttg cggccgcata atgcttaagt
     1501 ccatatggca gatctcaatt ggatatcggc cggccacgcg atcgctgacg tcggtaccct
     1561 cgagtgacta caaggaccac gacggcgact ataaggacca cgacatcgac tacaaggatg
     1621 acgacgacaa gtgagggccc tataatttgg tgaattggag ttattttcat atttttgcat
     1681 cccgaagagt ttctcttaaa gagagaaaca tcttttgcat accttttccg accgaatttt
     1741 tatgtcgtaa agaggggctt tgcagggggt ggactcagaa agatgagaat agatgactat
     1801 tgtagttgaa acacatagaa agttgctgat atacagaccg atacgcatat cgggatgaac
     1861 catgagtacg ttcttttctc aaaaaacata aatattcgaa aagagatgca ataaattaag
     1921 gagaggttat agcttagcat gacaaaaaag aaattgcccg ttcgttttac gggtcagcac
     1981 tttactattg ataaagtgct aataaaagat gcaataagac aagcaaatat aagtaatcag
     2041 gatacggttt tagatattgg ggcaggcaag gggtttctta ctgttcattt attaaaaatc
     2101 gccaacaatg ttgttgctat tgaaaacgac acagctttgg ttgaacattt acgaaaatta
     2161 ttttctgatg cccgaaatgt tcaagttgtc ggttgtgatt ttaggaattt tgcagttccg
     2221 aaatttcctt tcaaagtggt gtcaaatatt ccttatggca ttacttccga tattttcaaa
     2281 atcctgatgt ttgagagtct tggaaatttt ctgggaggtt ccattgtcct tcaattagaa
     2341 cctacacaaa agttattttc gaggaagctt tacaatccat ataccgtttt ctatcatact
     2401 ttttttgatt tgaaacttgt ctatgaggta ggtcctgaaa gtttcttgcc accgccaact
     2461 gtcaaatcag ccctgttaaa cattaaaaga aaacacttat tttttgattt taagtttaaa
     2521 gccaaatact tagcatttat ttcctatctg ttagagaaac ctgatttatc tgtaaaaaca
     2581 gctttaaagt cgattttcag gaaaagtcag gtcaggtcaa tttcggaaaa attcggttta
     2641 aaccttaatg ctcaaattgt ttgtttgtct ccaagtcaat ggttaaactg ttttttggaa
     2701 atgctggaag ttgtccctga aaaatttcat ccttcgtagg catgcggcgc tccccgaccg
     2761 atgattttta agtgactgat tttgtgctgt tttgggggta tattaagaat aaaagaaata
     2821 gaataagtta agtacttgat acacaatata gggcattttc catattggaa attctcattt
     2881 tccaatctag aaaataccga tttcctaata tgatactaaa taggaaaata atatttccct
     2941 taatattgtt tttatggaaa ataatagttt actttgtgga gaataatatt tcccaaaaac
     3001 atatcaaaat ggaaaataaa aaagcagtta agttaaccga ttttcaaaag aacgaagaaa
     3061 atccttttat gaaacaagct atagaaggta ttgaaaatca tgttgttaaa aagtataaga
     3121 gtaatagtgg tggcgataag agagctgtag tagctttagc cgacactgaa actggagaag
     3181 tgtttaagac ttcgtttatc cgtcaaatag aagtagatga agaacaattc actaaattgt
     3241 atctttctaa ctttgctgca ttctttgacc tatcacaagc agctattcgg gtttttggtt
     3301 actttatgac ctgcatgaaa cccaaaaatg atttaatcat tttcaataga aaaaaatgcc
     3361 tagaatatac caaatacaaa acagacaaag ccgtttataa aggacttgca gaacttgtaa
     3421 aagctgaaat catagcccga ggaccagccg ataatctttg gtttattaat cctctgatag
     3481 tattcaatgg tgaccgagtg acatttgcta aaacatacgt tcggaaaaag actttagctg
     3541 cccaaaagaa agaagaagca gagaaacgac aattatcact tggctttgat gaacagtaac
     3601 actccattga gtgaagctgc cgtttggtcg ctcccccttt gggggcgggg gggggataga
     3661 taaagttcct ctatgtaaag ttataatggg ggatgaaagg caaggttcgc taaccttacc
     3721 ccgattgttt atgcttccca gcattcaatc tatccccgtt tatgggtttc tctttcaccc
     3781 tacaaagata acctcatggg ggaaaaatgt ccaagaatat ggggtaaaaa ctatcaagtc
     3841 ggtagaaaat aagtatcttt gaagtacatt tttagtagag gtactggtgt gcctaggcga
     3901 agcagatagg caaaaataaa gccgctggag atgtcggatg tccgtcaatt aactccagcg
     3961 gcttgtattt tggtaactct cccgtatcgg tcagctaccg tttggtcagc aacaaaggta
     4021 gtactttatt atcgcaaatc caaatgtttt tccctttatt tgtgcattta caaaacattc
     4081 actaacttcg cacctatgca gtatggtgat agcacactag caccctaaag agtgcgtgtg
     4141 taaggggaca agccccctta accccctgtc agccggctgt caccggctgt tatcgattgt
     4201 cacaaaaatg ttaacttatg ggagcaacaa gtattcatgt acaagcagtg aagccgggga
     4261 gcgagattca caactttagg gaaaaagagt tggactatgt tcgtcccgaa cttagtcatt
     4321 tgaatgaaag ctgggttgga gatagcattt cccatcggct ggagagtgca aaacaaagat
     4381 gctagcatat agcgactttt ttacaaaatg ctaaaatttg ttgatttccg tcaagcaatt
     4441 gttgagcaaa aatgtctttt acgataaaat gatacctcaa tatcaactgt ttagcaaaac
     4501 gatatttctc ttaaagagag aaacaccttt ttgttcacca atccccgact tttaatcccg
     4561 cggccatgat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     4621 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     4681 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     4741 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     4801 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     4861 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     4921 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     4981 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     5041 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     5101 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     5161 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     5221 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     5281 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     5341 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     5401 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     5461 ttactcataa cgcgtcaatt cgagggggat caattccgtg ataggtgggc tgcccttcct
     5521 ggttggcttg gtttcatcag ccatccgctt gccctcatct gttacgccgg cggtagccgg
     5581 ccagcctcgc agagcaggat tcccgttgag caccgccagg tgcgaataag ggacagtgaa
     5641 gaaggaacac ccgctcgcgg gtgggcctac ttcacctatc ctgcccggct gacgccgttg
     5701 gatacaccaa ggaaagtcta cacgaaccct ttggcaaaat cctgtatatc gtgcgaaaaa
     5761 ggatggatat accgaaaaaa tcgctataat gaccccgaag cagggttatg cagcggaaaa
     5821 cggaattgat ccggccacga tgcgtccggc gtagag
//
