LOCUS       pMS07_(plasmidsaurus_ver_04/25/2025;_clone_A)        6049 bp    DNA     circular SYN 16-MAY-2025
DEFINITION  pMS07a.
ACCESSION   pMS07a
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     Annotated with pLannotate v1.2.2.
FEATURES             Location/Qualifiers
     rep_origin      1..389
     CDS             complement(408..1307)
                     /standard_name="BT_3768 RhaR repressor"
     regulatory      complement(1308..1422)
                     /note="Geneious type: promoter"
                     /standard_name="BT3768 promoter (RhaR)"
     regulatory      1423..1616
                     /note="Geneious type: promoter"
                     /standard_name="BT3763 promoter (rhamnose inducible)"
     misc_feature    1617..1757
                     /standard_name="MCS B"
     misc_feature    1759..1827
                     /note="Geneious type: motif"
                     /standard_name="3xFLAG"
     CDS             2132..2929
                     /note="pLannotate"
                     /standard_name="ermF"
     CDS             3058..3792
                     /standard_name="repA"
     CDS             4779..5639
                     /note="pLannotate"
                     /standard_name="AmpR"
     oriT            complement(5770..5879)
                     /note="pLannotate"
                     /standard_name="oriT"
ORIGIN      
        1 gatctgaaga tcagcagttc aacctgttga tagtacgtac taagctctca tgtttcacgt
       61 actaagctct catgtttaac gtactaagct ctcatgttta acgaactaaa ccctcatggc
      121 taacgtacta agctctcatg gctaacgtac taagctctca tgtttcacgt actaagctct
      181 catgtttgaa caataaaatt aatataaatc agcaacttaa atagcctcta aggttttaag
      241 ttttataaga aaaaaaagaa tatataaggc ttttaaagct tttaaggttt aacggttgtg
      301 gacaacaagc cagggatgta acgcactgag aagcccttag agcctctcaa agcaattttg
      361 agtgacacag gaacacttaa cggctgacat gggaattccc ctccacctta ttcttcccga
      421 ccgaacgagc ggtattcaag cggagtaagc ccggtacgtt ttttgaaaaa ggagaaaaaa
      481 tattcggtag actggaagcc cagaaaaaaa gaaatttctt ttactgactg ggaagttccc
      541 accaacattt gcttggcttt acgaagtttc agttcctgaa aatatttggc gggtgcatag
      601 ccggtatatt ccttaaaaac cctgcggaac catgagtaac tgatattcag tctcatcgcc
      661 agttcctccg gatcaatatt gccggataca ttttcattca taagtatctt tgcctgctcg
      721 atcttctgat cgacatcact catctcgaag accttattct tggaaatcga aagaatcata
      781 ccaatcatgt gaagtacgat tccggaaagg tattgctggg cagatatttt atccgcttct
      841 gccacttcca aagcgcggga aaacaaagat accagttctt cattaatacc gacttccaat
      901 atctgtcttt cctgagaaag aaaggcatcg cccacaatcg tatcaatcgc cggaccttca
      961 aaaccaatgt aatattccgt ccatccggtc tgccgcaatg gataataagt atgccactgt
     1021 ccgggaaaca acaccatcag ccgaccttta caaacttgtc tttcaggagt agaatcggaa
     1081 gaaaacaaac cgcgcccttt tgtaatatag accaattgat attcccgcaa tactcttcct
     1141 ttttcggcat tgaaatagta accggacgga tgctccttca gcggataagg agaatcggga
     1201 gggatagact gatagccaac cgtattcacc cacagaccga atttgcggtc catgtcatta
     1261 actatcaagt atttaaattc taatcccaaa ttattatcct cagtcataca ggcgtgtttt
     1321 catacaattg aattgaacaa aagtaggaat tattttcaaa aaacacacgc taaacagtaa
     1381 aaaaacatcc agagcacctg tccagatcct ccggatgctt attatttcgg agaaaacatg
     1441 cataaatcat gctttttttg cataaaaagt aaaatttata ctgatgtaag gtttggctat
     1501 gcagatttgt gtcaaaatgc acatcctttc tatcaaaatg cgtaaggaaa aggaggaagg
     1561 aaccgcctat ctttgcaatg taggtaaatg gataccttaa atatatagac aaaatacccg
     1621 ggcatgccag gatccgaatt cgagctcggc gcgcctgcag gtcgacaagc ttgcggccgc
     1681 ataatgctta agtccatatg gcagatctca attggatatc ggccggccac gcgatcgctg
     1741 acgtcggtac cctcgagtga ctacaaggac cacgacggcg actataagga ccacgacatc
     1801 gactacaagg atgacgacga caagtgaggg ccctataatt tggtgaattg gagttatttt
     1861 catatttttg catcccgaag agtttctctt aaagagagaa acatcttttg catacctttt
     1921 ccgaccgaat ttttatgtcg taaagagggg ctttgcaggg ggtggactca gaaagatgag
     1981 aatagatgac tattgtagtt gaaacacata gaaagttgct gatatacaga ccgatacgca
     2041 tatcgggatg aaccatgagt acgttctttt ctcaaaaaac ataaatattc gaaaagagat
     2101 gcaataaatt aaggagaggt tatagcttag catgacaaaa aagaaattgc ccgttcgttt
     2161 tacgggtcag cactttacta ttgataaagt gctaataaaa gatgcaataa gacaagcaaa
     2221 tataagtaat caggatacgg ttttagatat tggggcaggc aaggggtttc ttactgttca
     2281 tttattaaaa atcgccaaca atgttgttgc tattgaaaac gacacagctt tggttgaaca
     2341 tttacgaaaa ttattttctg atgcccgaaa tgttcaagtt gtcggttgtg attttaggaa
     2401 ttttgcagtt ccgaaatttc ctttcaaagt ggtgtcaaat attccttatg gcattacttc
     2461 cgatattttc aaaatcctga tgtttgagag tcttggaaat tttctgggag gttccattgt
     2521 ccttcaatta gaacctacac aaaagttatt ttcgaggaag ctttacaatc catataccgt
     2581 tttctatcat actttttttg atttgaaact tgtctatgag gtaggtcctg aaagtttctt
     2641 gccaccgcca actgtcaaat cagccctgtt aaacattaaa agaaaacact tattttttga
     2701 ttttaagttt aaagccaaat acttagcatt tatttcctat ctgttagaga aacctgattt
     2761 atctgtaaaa acagctttaa agtcgatttt caggaaaagt caggtcaggt caatttcgga
     2821 aaaattcggt ttaaacctta atgctcaaat tgtttgtttg tctccaagtc aatggttaaa
     2881 ctgttttttg gaaatgctgg aagttgtccc tgaaaaattt catccttcgt aggcatgcgg
     2941 cgctccccga ccgatgattt ttaagtgact gattttgtgc tgttttgggg gtatattaag
     3001 aataaaagaa atagaataag ttaagtactt gatacacaat atagggcatt ttccatattg
     3061 gaaattctca ttttccaatc tagaaaatac cgatttccta atatgatact aaataggaaa
     3121 ataatatttc ccttaatatt gtttttatgg aaaataatag tttactttgt ggagaataat
     3181 atttcccaaa aacatatcaa aatggaaaat aaaaaagcag ttaagttaac cgattttcaa
     3241 aagaacgaag aaaatccttt tatgaaacaa gctatagaag gtattgaaaa tcatgttgtt
     3301 aaaaagtata agagtaatag tggtggcgat aagagagctg tagtagcttt agccgacact
     3361 gaaactggag aagtgtttaa gacttcgttt atccgtcaaa tagaagtaga tgaagaacaa
     3421 ttcactaaat tgtatctttc taactttgct gcattctttg acctatcaca agcagctatt
     3481 cgggtttttg gttactttat gacctgcatg aaacccaaaa atgatttaat cattttcaat
     3541 agaaaaaaat gcctagaata taccaaatac aaaacagaca aagccgttta taaaggactt
     3601 gcagaacttg taaaagctga aatcatagcc cgaggaccag ccgataatct ttggtttatt
     3661 aatcctctga tagtattcaa tggtgaccga gtgacatttg ctaaaacata cgttcggaaa
     3721 aagactttag ctgcccaaaa gaaagaagaa gcagagaaac gacaattatc acttggcttt
     3781 gatgaacagt aacactccat tgagtgaagc tgccgtttgg tcgctccccc tttgggggcg
     3841 ggggggggat agataaagtt cctctatgta aagttataat gggggatgaa aggcaaggtt
     3901 cgctaacctt accccgattg tttatgcttc ccagcattca atctatcccc gtttatgggt
     3961 ttctctttca ccctacaaag ataacctcat gggggaaaaa tgtccaagaa tatggggtaa
     4021 aaactatcaa gtcggtagaa aataagtatc tttgaagtac atttttagta gaggtactgg
     4081 tgtgcctagg cgaagcagat aggcaaaaat aaagccgctg gagatgtcgg atgtccgtca
     4141 attaactcca gcggcttgta ttttggtaac tctcccgtat cggtcagcta ccgtttggtc
     4201 agcaacaaag gtagtacttt attatcgcaa atccaaatgt ttttcccttt atttgtgcat
     4261 ttacaaaaca ttcactaact tcgcacctat gcagtatggt gatagcacac tagcacccta
     4321 aagagtgcgt gtgtaagggg acaagccccc ttaaccccct gtcagccggc tgtcaccggc
     4381 tgttatcgat tgtcacaaaa atgttaactt atgggagcaa caagtattca tgtacaagca
     4441 gtgaagccgg ggagcgagat tcacaacttt agggaaaaag agttggacta tgttcgtccc
     4501 gaacttagtc atttgaatga aagctgggtt ggagatagca tttcccatcg gctggagagt
     4561 gcaaaacaaa gatgctagca tatagcgact tttttacaaa atgctaaaat ttgttgattt
     4621 ccgtcaagca attgttgagc aaaaatgtct tttacgataa aatgatacct caatatcaac
     4681 tgtttagcaa aacgatattt ctcttaaaga gagaaacacc tttttgttca ccaatccccg
     4741 acttttaatc ccgcggccat gattgaaaaa ggaagagtat gagtattcaa catttccgtg
     4801 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     4861 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     4921 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     4981 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     5041 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     5101 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     5161 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     5221 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     5281 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     5341 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     5401 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     5461 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     5521 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     5581 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     5641 tgtcagacca agtttactca taacgcgtca attcgagggg gatcaattcc gtgataggtg
     5701 ggctgccctt cctggttggc ttggtttcat cagccatccg cttgccctca tctgttacgc
     5761 cggcggtagc cggccagcct cgcagagcag gattcccgtt gagcaccgcc aggtgcgaat
     5821 aagggacagt gaagaaggaa cacccgctcg cgggtgggcc tacttcacct atcctgcccg
     5881 gctgacgccg ttggatacac caaggaaagt ctacacgaac cctttggcaa aatcctgtat
     5941 atcgtgcgaa aaaggatgga tataccgaaa aaatcgctat aatgaccccg aagcagggtt
     6001 atgcagcgga aaacggaatt gatccggcca cgatgcgtcc ggcgtagag
//
