LOCUS       75_fabp10a_DD-Cr        9831 bp    DNA     circular SYN 19-AUG-2024
DEFINITION  Clone #22 of ecDHFR based destable domain function at low 
            temperature for regulating protein stability in Caenorhabditis 
            elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 14, 2024 from SnapGene 8.0.0
            https://www.snapgene.com
COMMENT     Cho U, Zimmerman SM, Chen LC, Owen E, Kim JV, Kim SK, Wandless 
            TJ.Rapid and Tunable Control of Protein Stability in Caenorhabditis 
            elegans Using a Small Molecule.PLoS One. 2013 Aug 22;8(8):e72393. 
            doi: 10.1371/journal.pone.0072393.PubMed ID:  23991108
FEATURES             Location/Qualifiers
     source          1..9831
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(444..462)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(495..511)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(652..1107)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        1134..1238
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1239..1307,1308..2099)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2270..2858
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3146..3167
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3182..3187,3188..3205,3206..3212)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3220..3236
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3244..3260
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3306..3324
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        3339..5881
                     /label=fabp10a
     CDS             5902..6378
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /label=ecDHFR
                     /translation="MISLIAALAVDHVIGMENAMPWSLPADLAWFKRNTLNKPVIMGRH
                     TWESIGRPLPGRKNIILSSQPSTDDRVTWVKSADEAIAACGDVPEIMVIGGGRVYEQFL
                     PKAQKLYLTHIDAEVGGDTHFPDYEPDDWGSVFSEFHDADAQNSHSYCFVILGRR"
     CDS             6385..7410
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLL
                     SVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
                     GD"
     CDS             7423..8358
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             8368..8394
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    8578..8699
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    8742..8949
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(join(8973..9686,9687..9689,9690..9692))
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     promoter        complement(join(9695..9831,1..425))
                     /label=cryaa
ORIGIN
        1 cagacccctg gttatatacc tgtgctgcag aaagaggggc ttacaaatgg atctctctgg
       61 aatggcatta tctcatggtg ggaacagtgt tgtcagcaga atcagcgggg tggactctga
      121 cctgagaatg acagtctagc actgaatggc tcagacaata ctcttgtggc caactatcac
      181 cggaaaataa gtgcctgcca aaacaaacca gaccagatga agctacatgg ctgtagaacg
      241 tcagtattta atgtgttgtg gccacttggt tcaccagatt taagagctaa tttttttaaa
      301 tatcacagat aatcaatcgc tttttttttt cttgactgaa aagcttagac tggaaaattc
      361 tttgaaaaag tttgcactac agaaaataaa tacataacat cctgcactga atgcacacta
      421 ttaatggtac ccagcttttg ttccctttag tgagggttaa ttgcgcgcta gggataacag
      481 ggtaatacgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
      541 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
      601 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gaaattgtaa
      661 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
      721 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
      781 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
      841 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
      901 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
      961 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     1021 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     1081 cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa
     1141 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     1201 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     1261 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     1321 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     1381 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     1441 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     1501 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     1561 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     1621 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     1681 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     1741 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     1801 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     1861 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     1921 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     1981 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     2041 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     2101 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     2161 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     2221 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     2281 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     2341 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     2401 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     2461 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     2521 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     2581 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     2641 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     2701 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     2761 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     2821 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     2881 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     2941 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     3001 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     3061 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     3121 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     3181 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     3241 acacaggaaa cagctatgac catgattacg ccaagcgcgt tagggataac agggtaatgc
     3301 gcgcgtaata cgactcacta tagggcgaat tggagctctg cagaagagca tctctgaaca
     3361 cacaacacgt ccaaccttga ggcagatggg ctacagcagc agaagaccac accgggtgcc
     3421 gctcctgtca gctaagaaca ggaaactgag gctacaattc acacagactc accaaaactg
     3481 gacgatatat tattagcccc ccttagaatt tttcatttga taatattttt cttctggcga
     3541 aagcctcatt tgttttatat attatagaat aaaattagtt tttaatagtt tttatgccat
     3601 tttaaggtca atattattag cccctttaag ctattttttt tcgatagtct acagaacaaa
     3661 ccatcggtat acaatgactt gcctaattac cctaacctgc ctagttaccc taattaacct
     3721 agttaagcct ttaaatgtca ctttaagctg tatagaagtg tcttgaagaa tatctagtct
     3781 aatattattg actgtcatca tggcaaagat aaaataaatc agttattaaa actattatga
     3841 ttagaaatgt gctgaaacaa tctgctctcc gataaacaga aattgaacaa aataaacagg
     3901 ggggctaata aatttaaggg gttaaataat tctgattgca aaaaaaatga tgtctgcaaa
     3961 actgttgcaa ataatttatt tgtgttgatt ttaagcaaac aaattaaatt taataaaata
     4021 caacttaatc tgtttgttta aattcagccc taataaattg tttacagcca cttaacgtaa
     4081 aaaaattgag taaatccaag gaatcatctc tgaataattt ttttcagtgt atatatatat
     4141 atatatattc ttacaaaaca actcatttac tttagtttaa ttttcagggg caaaaaacta
     4201 aagtaatcga cgttgcttga ataaaaagtg taattaaggg aatgaggtaa catttaacca
     4261 tgtgtcaatg cagtttaaat atgccagtta gtggtatatg tttaaatggt aagctattca
     4321 aaactttaaa ctaacttaac cagccttttg ttgtcagact gaacagactt tccatctgca
     4381 ttattagaga ctaatctttg gctggatgaa tgattcatct gctgatattt cagaatagac
     4441 agattgaggc tgtttctaat atgattatgc aacctgaggg tgattatttg aagcaaactc
     4501 cacagaccag caggtcattg accgtcgtgt gttcaaacag agcagaaaca tttgcaaaac
     4561 tggtctgaca ggagaatcca gtccagcaca acacatatgc tgagcaaact gaatcaatcc
     4621 tgcaggtcaa ctctcgtgct ttaagtttat taagattatt ttatttattt attattttat
     4681 ttatctattt atttatttag ttgtttattt agcaattatt tgtttattta tttgtttatt
     4741 tattcctgca gatcatgcct tgtgcctttt tacatttaat ttaattttta atttaatttc
     4801 cttttacttt tttttatttt tttattttat tttattttac agtctgacaa atactgaact
     4861 aaaaaccctc agatcatgtc ttatgcattt cattttattt tatttcattt tatattatta
     4921 attttaatat ttttatttta cagtctgaca aatactgaat taaaaaccat cagatcatgt
     4981 ctcatgcatt taacttaact ttatttaatt caattaaatt gtttgtttgt ttgtttcctt
     5041 gcatttgttt gtttgttttt tacaatctga catactggac cgaaaaaact cagatcatgt
     5101 cttatgcatt ttattttatt ttatttttta ttttattgtc tattttattt tatttcatct
     5161 tattttattt tacagtctga catatggacc aataaatctc tcagatcatg tctcatgcat
     5221 ttaatttaat ttttttattt attcattttt atttatttat tttattattt attttatttt
     5281 aatatttatt ttattttatt ttattttatt ttacagtctg acatactgga ccaataaatc
     5341 tctcagatca tgtctcatgc attttacttt tattttatta gaattagaaa gatcaaagga
     5401 acaactttta aaatattaat tctgtatcaa aatctctttt gatacattta attgatttaa
     5461 aaaagcagtt cacccaagaa acatttcctc acagtcgaat ggttgtaaac ttttatgaat
     5521 tactttcaca gaaaaagatt tttggaagaa tattggaaaa aaagcagcca ttgacttcca
     5581 tagtaacaac aaaaaatact atggaagtca atggctgttt tttcaccatt cggtatcttc
     5641 attctggagc agaatttttt gggtgatctg tccctttaag tcgtcaaatc ctggtgcaat
     5701 attccacatg cactgacgag tctttatttt tggtctgcta ctgctgtgtg tgtgagggca
     5761 tttacctcat cttctgctgg agttgatgaa cggtgggttg ttcaaacagc agcaggtcat
     5821 tgactgaact cctctcgata taaaagctgc agatctgaag ctgaccttca ctttgtgttg
     5881 atcgctagcg aattcgccac catgatcagt ctgattgcgg cgttagcggt agatcacgtt
     5941 atcggcatgg aaaacgccat gccgtggagc ctgcctgccg atctcgcctg gtttaaacgc
     6001 aacaccttaa ataaacccgt gattatgggc cgccatacct gggaatcaat cggtcgtccg
     6061 ttgccaggac gcaaaaacat tatcctcagc agtcaaccga gtacggacga tcgcgtaacg
     6121 tgggtgaagt cggcggatga agccatcgcg gcgtgtggtg acgtaccaga aatcatggtg
     6181 attggcggcg gtcgcgttta tgagcagttc ttgccaaaag cgcaaaaact gtatctgacg
     6241 catatcgacg cagaagtggg aggcgacacc catttcccgg attacgagcc ggatgactgg
     6301 ggatcggtat tcagcgagtt ccacgatgct gatgcgcaga actctcacag ctattgcttt
     6361 gtgattctgg ggcggcgagc atgctccaat ttactgaccg tacaccaaaa tttgcctgca
     6421 ttaccggtcg atgcaacgag tgatgaggtt cgcaagaacc tgatggacat gttcagggat
     6481 cgccaggcgt tttctgagca tacctggaaa atgcttctgt ccgtttgccg gtcgtgggcg
     6541 gcatggtgca agttgaataa ccggaaatgg tttcccgcag aacctgaaga tgttcgcgat
     6601 tatcttctat atcttcaggc gcgcggtctg gcagtaaaaa ctatccagca acatttgggc
     6661 cagctaaaca tgcttcatcg tcggtccggg ctgccacgac caagtgacag caatgctgtt
     6721 tcactggtta tgcggcggat ccgaaaagaa aacgttgatg ccggtgaacg tgcaaaacag
     6781 gctctagcgt tcgaacgcac tgatttcgac caggttcgtt cactcatgga aaatagcgat
     6841 cgctgccagg atatacgtaa tctggcattt ctggggattg cttataacac cctgttacgt
     6901 atagccgaaa ttgccaggat cagggttaaa gatatctcac gtactgacgg tgggagaatg
     6961 ttaatccata ttggcagaac gaaaacgctg gttagcaccg caggtgtaga gaaggcactt
     7021 agcctggggg taactaaact ggtcgagcga tggatttccg tctctggtgt agctgatgat
     7081 ccgaataact acctgttttg ccgggtcaga aaaaatggtg ttgccgcgcc atctgccacc
     7141 agccagctat caactcgcgc cctggaaggg atttttgaag caactcatcg attgatttac
     7201 ggcgctaagg atgactctgg tcagagatac ctggcctggt ctggacacag tgcccgtgtc
     7261 ggagccgcgc gagatatggc ccgcgctgga gtttcaatac cggagatcat gcaagctggt
     7321 ggctggacca atgtaaatat tgtcatgaac tatatccgta acctggatag tgaaacaggg
     7381 gcaatggtgc gcctgctgga agatggcgat ctcgagccat ctgctggaga catgagagct
     7441 gccaaccttt ggccaagccc gctcatgatc aaacgctcta agaagaacag cctggccttg
     7501 tccctgacgg ccgaccagat ggtcagtgcc ttgttggatg ctgagccccc catactctat
     7561 tccgagtatg atcctaccag acccttcagt gaagcttcga tgatgggctt actgaccaac
     7621 ctggcagaca gggagctggt tcacatgatc aactgggcga agagggtgcc aggctttgtg
     7681 gatttgaccc tccatgatca ggtccacctt ctagaatgtg cctggctaga gatcctgatg
     7741 attggtctcg tctggcgctc catggagcac ccagtgaagc tactgtttgc tcctaacttg
     7801 ctcttggaca ggaaccaggg aaaatgtgta gagggcatgg tggagatctt cgacatgctg
     7861 ctggctacat catctcggtt ccgcatgatg aatctgcagg gagaggagtt tgtgtgcctc
     7921 aaatctatta ttttgcttaa ttctggagtg tacacatttc tgtccagcac cctgaagtct
     7981 ctggaagaga aggaccatat ccaccgagtc ctggacaaga tcacagacac tttgatccac
     8041 ctgatggcca aggcaggcct gaccctgcag cagcagcacc agcggctggc ccagctcctc
     8101 ctcatcctct cccacatcag gcacatgagt aacaaaggca tggagcatct gtacagcatg
     8161 aagtgcaaga acgtggtgcc cctctatgac ctgctgctgg aggcggcgga cgcccaccgc
     8221 ctacatgcgc ccactagccg tggaggggca tccgtggagg agacggacca aagccacttg
     8281 gccactgcgg gctctacttc atcgcattcc ttgcaaaagt attacatcac gggggaggca
     8341 gagggtttcc ctgccacagc tgtcgactac ccatacgacg taccagatta cgcttgatta
     8401 attaagtaag tttaaactga gttctactaa ctaacgagta atatttaaat tttcaggcgg
     8461 ccgcgactct agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     8521 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     8581 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     8641 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa
     8701 tcgagggggg gcccggtacc caccgcggtg gcggcgatct ctccccagca tgcctgctat
     8761 tgtcttccca atcctccccc ttgctgtcct gccccacccc accccccaga atagaatgac
     8821 acctactcag acaatgcgat gcaatttcct cattttatta ggaaaggaca gtgggagtgg
     8881 caccttccag ggtcaaggaa ggcacggggg aggggcaaac aacagatggc tggcaactag
     8941 aaggcacagt cgaggctgat cagcgggccg ctttacttgt acagctcgtc catgccgaga
     9001 gtgatcccgg cggcggtcac gaactccagc aggaccatgt gatcgcgctt ctcgttgggg
     9061 tctttgctca gcttggactg ggtgctcagg tagtggttgt cgggcagcag cacggggccg
     9121 tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg
     9181 ttgtggcgga tcttgaagtt ggccttgatg ccgttcttct gcttgtcggc ggtgatatag
     9241 acgttgtcgc tgatggcgtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg
     9301 aagtcgatgc ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg
     9361 gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct
     9421 tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta gcgggcgaag
     9481 cactgcacgc cccaggtcag ggtggtcacg agggtgggcc agggcacggg cagcttgccg
     9541 gtggtgcaga tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc gccctcgccg
     9601 gacacgctga acttgtggcc gtttacgtcg ccgtccagct cgaccaggat gggcaccacc
     9661 ccggtgaaca gctcctcgcc cttgctcacc atgttgtcag acctggtaac tccttactgt
     9721 aacttattgc acctgatgaa caaaagatgt gaactgaacg caagtaggag gctaatagag
     9781 gctagtacag tacaaagatc cccaaatgat gtgtgccggc cttagtggag g
//
