LOCUS       CAG-FRT-MCS-WPRE-SV40_1__seq_1520_ 6208 bp    DNA     circular SYN 05-OCT-2024
DEFINITION  CAG-FRT-MCS-WPRE-SV40_1__seq_1520_.
ACCESSION   CAG-FRT-MCS-WPRE-SV40_1__seq_1520_
VERSION     CAG-FRT-MCS-WPRE-SV40_1__seq_1520_
KEYWORDS    .
SOURCE      
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     misc_feature    complement(1390..1978)
                     /note="pLannotate"
                     /label="WPRE"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     rep_origin      4336..4716
                     /note="pLannotate"
                     /label="M13 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     enhancer        complement(3548..3927)
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     repeat_region   3957..4097
                     /note="pLannotate"
                     /label="AAV2 ITR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="repeat_region"
     repeat_region   complement(1007..1147)
                     /note="pLannotate"
                     /label="AAV2 ITR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="repeat_region"
     polyA_signal    1253..1374
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     misc_feature    complement(2033..2140)
                     /note="pLannotate"
                     /label="MCS"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     promoter        5073..5177
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     intron          complement(2249..3265)
                     /note="pLannotate"
                     /label="chimeric intron"
                     /database="snapgene"
                     /identity="99.9"
                     /match_length="100.0"
                     /fragment="False"
                     /other="intron"
     CDS             5178..6038
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      1..589
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     protein_bind    1985..2032
                     /note="pLannotate"
                     /label="FRT"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     protein_bind    complement(2141..2188)
                     /note="pLannotate"
                     /label="FRT"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     promoter        913..943
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        complement(3266..3546)
                     /note="pLannotate"
                     /label="chicken β-actin promoter"
                     /database="snapgene"
                     /identity="98.9"
                     /match_length="101.1"
                     /fragment="False"
                     /other="promoter"
     protein_bind    877..898
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     protein_bind    951..967
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             4106..4260
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="89.1"
                     /fragment="True"
                     /other="CDS"
     misc_feature    complement(4266..4317)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="36.9"
                     /fragment="True"
                     /other="misc_feature"
     misc_feature    complement(4792..4841)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="35.5"
                     /fragment="True"
                     /other="misc_feature"
     CDS             772..864
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(4943..4978)
                     /note="pLannotate"
                     /label="rop (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="18.8"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(2183..2251)
                     /note="pLannotate"
                     /label="HBB (fragment)"
                     /database="swissprot"
                     /identity="82.6"
                     /match_length="15.6"
                     /fragment="True"
                     /other="CDS"
     CDS             1162..1230
                     /note="pLannotate"
                     /label="large T antigen (fragment)"
                     /database="snapgene"
                     /identity="98.6"
                     /match_length="3.2"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gaattgcctg caggcagctg
     1021 cgcgctcgct cgctcactga ggccgcccgg gcaaagcccg ggcgtcgggc gacctttggt
     1081 cgcccggcct cagtgagcga gcgagcgcgc agagagggag tggccaactc catcactagg
     1141 ggttcctatc gatggcgcgc ctaccacatt tgtagaggtt ttacttgctt taaaaaacct
     1201 cccacatctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
     1261 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     1321 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     1381 ctgtgtacag cggggaggcg gcccaaaggg agatccgact cgtctgaggg cgaaggcgaa
     1441 gacgcggaag aggccgcaga gccggcagca ggccgcggga aggaaggtcc gctggattga
     1501 gggccgaagg gacgtagcag aaggacgtcc cgcgcagaat ccaggtggca acacaggcga
     1561 gcagccaagg aaaggacgat gatttccccg acaacaccac ggaattgtca gtgcccaaca
     1621 gccgagcccc tgtccagcag cgggcaaggc aggcggcgat gagttccgcc gtggcaatag
     1681 ggagggggaa agcgaaagtc ccggaaagga gctgacaggt ggtggcaatg ccccaaccag
     1741 tgggggttgc gtcagcaaac acagtgcaca ccacgccacg ttgcctgaca acgggccaca
     1801 actcctcata aagagacagc aaccaggatt tatacaagga ggagaaaatg aaagccatac
     1861 gggaagcaat agcatgatac aaaggcatta aagcagcgta tccacatagc gtaaaaggag
     1921 caacatagtt aagaatacca gtcaatcttt cacaaatttt gtaatccaga ggttgattgc
     1981 tagcgaagtt cctattccga agttcctatt ctctagaaag tataggaact tcggtaccgg
     2041 gccccccctc gaggtcgacg gtatcgataa gcttgatatc gaattcctgc agcccggggg
     2101 atccactagt tctagagcgg ccgccaccgc ggtggagctc gaagttccta tactttctag
     2161 agaataggaa cttcggaata ggaacttcac cggtcaattc tttgccaaaa tgatgagaca
     2221 gcacaataac cagcacgttg cccaggagct gtaggaaaaa gaagaaggca tgaacatggt
     2281 tagcagaggc tctagagccg ccggtcacac gccagaagcc gaaccccgcc ctgccccgtc
     2341 ccccccgaag gcagccgtcc ccccgcggac agccccgagg ctggagaggg agaaggggac
     2401 ggcggcgcgg cgacgcacga aggccctccc cgcccatttc cttcctgccg gcgccgcacc
     2461 gcttcgcccc gcgcccgcta gagggggtgc ggcggcgcct cccagatttc ggctccgcac
     2521 agatttggga caaaggaagt ccctgcgccc tctcgcacga ttaccataaa aggcaatggc
     2581 tgcggctcgc cgcgcctcga cagccgccgg cgctccgggg gccgccgcgc ccctcccccg
     2641 agccctcccc ggcccgaggc ggccccgccc cgcccggcac ccccacctgc cgccaccccc
     2701 cgcccggcac ggcgagcccc gcgccacgcc ccgtacggag ccccgcaccc gaagccgggc
     2761 cgtgctcagc aactcgggga ggggggtgca gggggggttg cagcccgacc gacgcgccca
     2821 caccccctgc tcaccccccc acgcacacac cccgcacgca gcctttgttc ccctcgcagc
     2881 cccccccgca ccgcggggca ccgcccccgg ccgcgctccc ctcgcgcaca ctgcggagcg
     2941 cacaaagccc cgcgccgcgc ccgcagcgct cacagccgcc gggcagcgcg gagccgcacg
     3001 cggcgctccc cacgcacaca cacacgcacg caccccccga gccgctcccc ccgcacaaag
     3061 ggccctcccg gagcccctca aggctttcac gcagccacag aaaagaaaca agccgtcatt
     3121 aaaccaagcg ctaattacag cccggaggag aagggccgtc ccgcccgctc acctgtggga
     3181 gtaacgcggt cagtcagagc cggggcgggc ggcgcgaggc ggcggcggag cggggcacgg
     3241 ggcgaaggca gcgcgcagcg actcccgccc gccgcgcgct tcgcttttta tagggccgcc
     3301 gccgccgccg cctcgccata aaaggaaact ttcggagcgc gccgctctga ttggctgccg
     3361 ccgcacctct ccgcctcgcc ccgccccgcc cctcgccccg ccccgccccg cctggcgcgc
     3421 gccccccccc cccccccccc gcccccatcg ctgcacaaaa taattaaaaa ataaataaat
     3481 acaaaattgg gggtggggag gggggggaga tggggagagt gaagcagaac gtggggctca
     3541 cctcgaccat ggtaatagcg atgactaata cgtagatgta ctgccaagta ggaaagtccc
     3601 ataaggtcat gtactgggca taatgccagg cgggccattt accgtcattg acgtcaatag
     3661 ggggcgtact tggcatatga tacacttgat gtactgccaa gtgggcagtt taccgtaaat
     3721 actccaccca ttgacgtcaa tggaaagtcc ctattggcgt tactatggga acatacgtca
     3781 ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa
     3841 gttatgtaac gcggaactcc atatatgggc tatgaactaa tgaccccgta attgattact
     3901 attaataact agtcaataat caatgtcgag ggttaccgcc taggatcgat agatctagga
     3961 acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg
     4021 gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc
     4081 gcgcagctgc ctgcaggcag cttggcactg gccgtcgttt tacaacgtcg tgactgggaa
     4141 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     4201 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     4261 tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt
     4321 caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     4381 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     4441 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     4501 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg
     4561 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     4621 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcgggct
     4681 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     4741 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca
     4801 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
     4861 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     4921 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
     4981 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     5041 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     5101 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     5161 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     5221 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     5281 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     5341 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     5401 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     5461 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     5521 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     5581 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     5641 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     5701 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     5761 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     5821 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     5881 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     5941 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     6001 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     6061 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     6121 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     6181 accccgtaga aaagatcaaa ggatcttc
//
