LOCUS       CN2909_pAAV-3xc2_eHGT_445h-minBglobin-SYFP2-WPRE3-BGHpA        4897 bp    DNA     circular UNA 26-SEP-2020
DEFINITION  .
ACCESSION   urn.local...9l-cgep1l9
VERSION     urn.local...9l-cgep1l9
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     ##Comment-START##
            Comment :: rAAV-ESYN-SYFP2-WPRE3-BGHpA
                       clone #8-1 correct , 8-2 backup
            ##Comment-END##
            ##Imported_From-START##
            Path     :: C:\VNTI Database
            Filename :: _exdata.ma4
            ##Imported_From-END##
FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /label="L-ITR"
     misc_feature    156..821
                     /Original_Bases="ATAGGTACCGAGCTCTATGCACTCACAGTGGTTTGGCATGC
                     ATCTGGTGAATTTTTTTTAACGAAAAATTAGTGTTGGTTTCGATGTATGGTAGCATTC
                     TCCCTAACGTAATTTGAATAATTCAGCAAAGCCCCACTACCAGCTGTACTTCTGCAGC
                     CTCTTCCATTCTTTTCAGCATTATAATTTTGGTTAATTTTCAATTTTAGGTCCTACGT
                     CTCTGCAATTTGTGTATGAATAACAGAATAATTTCCCTCTTTTGTTTCGCCTTTCCTG
                     TTCCTGAATCTAAATAAAGATGGCTTTTTAGTATTAAAAGTGGAAGAAAATTACAGGT
                     AATTATCTTTGACGGTAAAAACGCTGTAATCAGCGGGCTACATGAAAAATTACTCTAA
                     TTATGGCTGCATTTAAGAGAATGGAAAAAAACCTTCTTGTGGATAAAAACCTTAAATT
                     GTCCCCAATGTCTGCTTCAAATTGGATGGCACTGCAGCTGGAGGCTTTGTTCAGAATT
                     GATCCTGGGGAGCTACGAACCCAAAGTTTCACAGTAGG"
                     /label="ATAGGTACCGAGCTCTATGCACTCACAGTGGTTTGGCATGCATCTGGTGA
                     ATTTTTTTTAACGAAAAATTAGTGTTGGTTTCGATGTATGGTAGCATTCTCCCTAACG
                     TAATTTGAATAATTCAGCAAAGCCCCACTACCAGCTGTACTTCTGCAGCCTCTTCCAT
                     TCTTTTCAGCATTATAATTTTGGTTAATTTTCAATTTTAGGTCCTACGTCTCTGCAAT
                     TTGTGTATGAATAACAGAATAATTTCCCTCTTTTGTTTCGCCTTTCCTGTTCCTGAAT
                     CTAAATAAAGATGGCTTTTTAGTATTAAAAGTGGAAGAAAATTACAGGTAATTATCTT
                     TGACGGTAAAAACGCTGTAATCAGCGGGCTACATGAAAAATTACTCTAATTATGGCTG
                     CATTTAAGAGAATGGAAAAAAACCTTCTTGTGGATAAAAACCTTAAATTGTCCCCAAT
                     GTCTGCTTCAAATTGGATGGCACTGCAGCTGGAGGCTTTGTTCAGAATTGATCCTGGG
                     GAGCTACGAACCCAAAGTTTCACAGTAGG"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    156..821
                     /created_by="ximena.opitzaraya"
                     /label="3xc2_eHGT_445h"
     misc_feature    828..880
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Beta Globin minimal promoter"
     misc_feature    837..842
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="TATA box"
     misc_feature    869
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="transcriptional start"
     misc_feature    921..1640
                     /label="SYFP2"
     primer_bind     967..987
                     /label="EGFP-N-R"
     misc_feature    1675..1920
                     /label="WPRE3"
     misc_feature    1934..2137
                     /label="BGH-pA"
     primer          complement(2048..2067)
                     /label="BGH-R"
     misc_feature    2160..2300
                     /label="R-ITR"
     rep_origin      2392..2698
                     /label="f1 Origin"
     misc_feature    3217..4074
                     /label="AmpR"
     rep_origin      4225..4892
                     /label="pUC ori"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtgga ggactttgag cttaacccta
      181 ttaaatttaa tctaatatat tcagcagcct gtcacaatct tgttaaatct ttattccgtc
      241 acccacctta ttagggggcc ctcccacttt agtcatcagc agattagtaa gcatgccatt
      301 tagatgtcat ctaagttaat gaacctgact gagccagagt cacatggcag agtcacagaa
      361 actccttctg agctggcgtg gaggactttg agcttaaccc tattaaattt aatctaatat
      421 attcagcagc ctgtcacaat cttgttaaat ctttattccg tcacccacct tattaggggg
      481 ccctcccact ttagtcatca gcagattagt aagcatgcca tttagatgtc atctaagtta
      541 atgaacctga ctgagccaga gtcacatggc agagtcacag aaactccttc tgagctggcg
      601 tggaggactt tgagcttaac cctattaaat ttaatctaat atattcagca gcctgtcaca
      661 atcttgttaa atctttattc cgtcacccac cttattaggg ggccctccca ctttagtcat
      721 cagcagatta gtaagcatgc catttagatg tcatctaagt taatgaacct gactgagcca
      781 gagtcacatg gcagagtcac agaaactcct tctgagctgg cgagctcggg ctgggcataa
      841 aagtcagggc agagccatct attgcttaca tttgcttctg ggatccagat ctttcgaagc
      901 tagcgctacc ggtcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc
      961 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     1021 gcgagggcga tgccacctac ggcaagctga ccctgaagct gatctgcacc accggcaagc
     1081 tgcccgtgcc ctggcccacc ctcgtgacca ccctgggcta cggcgtgcag tgcttcgccc
     1141 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     1201 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     1261 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     1321 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     1381 ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg
     1441 acggcggcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     1501 tgctgctgcc cgacaaccac tacctgagct accagtccaa gctgagcaaa gaccccaacg
     1561 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     1621 tggacgagct gtacaagtaa gtcgacggcg cgccgcggcc gcgaattcga tatcataatc
     1681 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     1741 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     1801 ctttcatttt ctcctccttg tataaatcct ggttagttct tgccacggcg gaactcatcg
     1861 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     1921 ctcgagagat cttcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     1981 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     2041 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     2101 agcaaggggg aggattggga agacaatagc aggcatgcac gtgcggaccg agcggccgca
     2161 ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc
     2221 cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg
     2281 agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     2341 tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc
     2401 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     2461 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     2521 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     2581 aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     2641 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     2701 ctcaacccta tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     2761 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     2821 tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     2881 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     2941 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     3001 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     3061 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     3121 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     3181 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     3241 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     3301 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     3361 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     3421 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     3481 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     3541 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     3601 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     3661 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     3721 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     3781 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     3841 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     3901 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     3961 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     4021 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     4081 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     4141 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     4201 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     4261 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     4321 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     4381 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     4441 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     4501 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     4561 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     4621 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     4681 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     4741 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     4801 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     4861 cggttcctgg ccttttgctg gccttttgct cacatgt
//
