LOCUS       CN3985_pAAV-3xc2_eHGT_445h-minBglobin-jRGECO1a-mod-WPRE3-BGHpA        5560 bp    DNA     circular UNA 25-FEB-2022
DEFINITION  .
ACCESSION   urn.local...8z-eewgm0n
VERSION     urn.local...8z-eewgm0n
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     ##Comment-START##
            Comment :: rAAV-ESYN-SYFP2-WPRE3-BGHpA
                       clone #8-1 correct , 8-2 backup
            ##Comment-END##
            ##Imported_From-START##
            Path     :: C:\VNTI Database
            Filename :: _exdata.ma4
            ##Imported_From-END##
FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /label="L-ITR"
     misc_feature    156..821
                     /Original_Bases="ATAGGTACCGAGCTCTATGCACTCACAGTGGTTTGGCATGC
                     ATCTGGTGAATTTTTTTTAACGAAAAATTAGTGTTGGTTTCGATGTATGGTAGCATTC
                     TCCCTAACGTAATTTGAATAATTCAGCAAAGCCCCACTACCAGCTGTACTTCTGCAGC
                     CTCTTCCATTCTTTTCAGCATTATAATTTTGGTTAATTTTCAATTTTAGGTCCTACGT
                     CTCTGCAATTTGTGTATGAATAACAGAATAATTTCCCTCTTTTGTTTCGCCTTTCCTG
                     TTCCTGAATCTAAATAAAGATGGCTTTTTAGTATTAAAAGTGGAAGAAAATTACAGGT
                     AATTATCTTTGACGGTAAAAACGCTGTAATCAGCGGGCTACATGAAAAATTACTCTAA
                     TTATGGCTGCATTTAAGAGAATGGAAAAAAACCTTCTTGTGGATAAAAACCTTAAATT
                     GTCCCCAATGTCTGCTTCAAATTGGATGGCACTGCAGCTGGAGGCTTTGTTCAGAATT
                     GATCCTGGGGAGCTACGAACCCAAAGTTTCACAGTAGG"
                     /label="ATAGGTACCGAGCTCTATGCACTCACAGTGGTTTGGCATGCATCTGGTGA
                     ATTTTTTTTAACGAAAAATTAGTGTTGGTTTCGATGTATGGTAGCATTCTCCCTAACG
                     TAATTTGAATAATTCAGCAAAGCCCCACTACCAGCTGTACTTCTGCAGCCTCTTCCAT
                     TCTTTTCAGCATTATAATTTTGGTTAATTTTCAATTTTAGGTCCTACGTCTCTGCAAT
                     TTGTGTATGAATAACAGAATAATTTCCCTCTTTTGTTTCGCCTTTCCTGTTCCTGAAT
                     CTAAATAAAGATGGCTTTTTAGTATTAAAAGTGGAAGAAAATTACAGGTAATTATCTT
                     TGACGGTAAAAACGCTGTAATCAGCGGGCTACATGAAAAATTACTCTAATTATGGCTG
                     CATTTAAGAGAATGGAAAAAAACCTTCTTGTGGATAAAAACCTTAAATTGTCCCCAAT
                     GTCTGCTTCAAATTGGATGGCACTGCAGCTGGAGGCTTTGTTCAGAATTGATCCTGGG
                     GAGCTACGAACCCAAAGTTTCACAGTAGG"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    156..821
                     /created_by="ximena.opitzaraya"
                     /label="3xc2_eHGT_445h"
     misc_feature    828..880
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Beta Globin minimal promoter"
     misc_feature    837..842
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="TATA box"
     misc_feature    869
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="transcriptional start"
     misc_feature    888..2309
                     /Original_Bases="GATCTTTCGAAGCTAGCGCTACCGGTCGCCACCATGGTGAG
                     CAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGAC
                     GTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCA
                     AGCTGACCCTGAAGCTGATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCT
                     CGTGACCACCCTGGGCTACGGCGTGCAGTGCTTCGCCCGCTACCCCGACCACATGAAG
                     CAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCT
                     TCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACAC
                     CCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTG
                     GGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCACCGCCGACAAGC
                     AGAAGAACGGCATCAAGGCCAACTTCAAGATCCGCCACAACATCGAGGACGGCGGCGT
                     GCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTG
                     CCCGACAACCACTACCTGAGCTACCAGTCCAAGCTGAGCAAAGACCCCAACGAGAAGC
                     GCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGA
                     CGAGCTGTACAAGTAAGTCGAC"
                     /label="GATCTTTCGAAGCTAGCGCTACCGGTCGCCACCATGGTGAGCAAGGGCGA
                     GGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGC
                     CACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCC
                     TGAAGCTGATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCAC
                     CCTGGGCTACGGCGTGCAGTGCTTCGCCCGCTACCCCGACCACATGAAGCAGCACGAC
                     TTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGG
                     ACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAA
                     CCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAG
                     CTGGAGTACAACTACAACAGCCACAACGTCTATATCACCGCCGACAAGCAGAAGAACG
                     GCATCAAGGCCAACTTCAAGATCCGCCACAACATCGAGGACGGCGGCGTGCAGCTCGC
                     CGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAAC
                     CACTACCTGAGCTACCAGTCCAAGCTGAGCAAAGACCCCAACGAGAAGCGCGATCACA
                     TGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTA
                     CAAGTAAGTCGAC"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    897..2309
                     /vntifkey=21
                     /label="jRGECO1a-mod"
     misc_feature    2338..2583
                     /label="WPRE3"
     misc_feature    2597..2800
                     /label="BGH-pA"
     primer          complement(2711..2730)
                     /label="BGH-R"
     misc_feature    2823..2963
                     /label="R-ITR"
     rep_origin      3055..3361
                     /label="f1 Origin"
     misc_feature    3880..4737
                     /label="AmpR"
     rep_origin      4888..5555
                     /label="pUC ori"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtgga ggactttgag cttaacccta
      181 ttaaatttaa tctaatatat tcagcagcct gtcacaatct tgttaaatct ttattccgtc
      241 acccacctta ttagggggcc ctcccacttt agtcatcagc agattagtaa gcatgccatt
      301 tagatgtcat ctaagttaat gaacctgact gagccagagt cacatggcag agtcacagaa
      361 actccttctg agctggcgtg gaggactttg agcttaaccc tattaaattt aatctaatat
      421 attcagcagc ctgtcacaat cttgttaaat ctttattccg tcacccacct tattaggggg
      481 ccctcccact ttagtcatca gcagattagt aagcatgcca tttagatgtc atctaagtta
      541 atgaacctga ctgagccaga gtcacatggc agagtcacag aaactccttc tgagctggcg
      601 tggaggactt tgagcttaac cctattaaat ttaatctaat atattcagca gcctgtcaca
      661 atcttgttaa atctttattc cgtcacccac cttattaggg ggccctccca ctttagtcat
      721 cagcagatta gtaagcatgc catttagatg tcatctaagt taatgaacct gactgagcca
      781 gagtcacatg gcagagtcac agaaactcct tctgagctgg cgagctcggg ctgggcataa
      841 aagtcagggc agagccatct attgcttaca tttgcttctg ggatccaccc gccaccatgc
      901 tgcagaacga gcttgctctt aagttggctg gacttgatat taacaagact ggaggaggtt
      961 ctcatcatca tcatcatcat ggtatggcaa gcatgactgg tggacagcaa atgggtcggg
     1021 atctgtacga cgatgacgat aaggatctcg caacaatggt cgactcatca cgtcgtaagt
     1081 ggaataaggc aggtcacgca gtcagagcta taggtcggct gagctccccc gtggtttccg
     1141 agcggatgta ccccgaggac ggcgccctga agagcgagat caagaagggg ctgaggctga
     1201 aggacggcgg ccactacgcc gccgaggtca agaccaccta caaggccaag aagcccgtgc
     1261 agctgcccgg cgcctacatc gtggacatca agttggacat cgtgtcccac aacgaggact
     1321 acaccatcgt ggaacagtgc gaacgcgccg agggccgcca ctccaccggc ggcatggacg
     1381 agctgtacaa gggaggtaca ggcgggagtc tggtgagcaa gggcgaggag gataacatgg
     1441 ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg
     1501 agttcgagat cgagggcgag ggcgagggcc gcccctacga ggcctttcag accgctaagc
     1561 tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca
     1621 tgtacggctc caaggcctac attaagcacc cagccgacat ccccgactac ttcaagctgt
     1681 ccttccccga gggcttcagg tgggagcgcg tgatgaactt cgaggacggc ggcattattc
     1741 acgtgaacca ggactcctcc ctgcaggacg gcgtattcat ctacaaggtg aagctgcgcg
     1801 gcaccaactt cccccccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcta
     1861 ctcgtgacga cctgactgaa gagcagatcg cagaatttaa agaggctttc tccctatttg
     1921 acaaggacgg ggatgggacg ataacaacca aggagcttgg gacggtgttc cgatcgctgg
     1981 ggcagaaccc cacagaagca gagctgcagg acatgatcaa tgaagtagat gccgacggtg
     2041 acggcacatt cgacttccct gagttcctga cgatgatggc aagaaaaatg aatgacacag
     2101 acagtgaaga ggaaattcgc gaagcgttcc gcgtgtttga taaggacggc aatggctaca
     2161 tcggcgcagc agagcttcgc cacgtgatga cagaccttgg agagaagtta acagatgagg
     2221 aggttgatga aatgatcagg gtagcagaca tcgatgggga tggtcaggta aactacgaag
     2281 agtttgtaca aatgatgaca gcgaagtgag gcgcgccgcg gccgcgaatt cgatatcata
     2341 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     2401 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     2461 tggctttcat tttctcctcc ttgtataaat cctggttagt tcttgccacg gcggaactca
     2521 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     2581 tggctcgaga gatcttcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     2641 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     2701 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     2761 gacagcaagg gggaggattg ggaagacaat agcaggcatg cacgtgcgga ccgagcggcc
     2821 gcaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga
     2881 ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga
     2941 gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat tttctcctta cgcatctgtg
     3001 cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta
     3061 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     3121 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     3181 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     3241 aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     3301 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     3361 acactcaacc ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggcc
     3421 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     3481 acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     3541 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     3601 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     3661 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     3721 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     3781 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     3841 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     3901 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     3961 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     4021 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     4081 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     4141 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     4201 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     4261 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     4321 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     4381 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     4441 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     4501 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     4561 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     4621 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     4681 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     4741 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     4801 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     4861 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     4921 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     4981 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     5041 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     5101 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     5161 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     5221 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     5281 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     5341 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     5401 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     5461 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     5521 ttacggttcc tggccttttg ctggcctttt gctcacatgt
//
