LOCUS       CN5571_pAAV-eHGT_1426m-minBG-iCre(R297T)-BGHpA        5094 bp    DNA     linear       26-APR-2024
DEFINITION  .
ACCESSION   urn.local...2u-hk16jta
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     rAAV-hI56i-minBG-IRES2-iCre(V318K)-BGHpA                           
            clone #1,2 correct                                                 
                                                                               
                                                                               
                                                                               
                                                                               
                                                                               
                                                                               
            This file is created by Vector NTI                                 
            http://www.invitrogen.com/                                         
            ORIGDB|GenBank                                                     
            VNTDATE|-13763174|                                                 
            VNTDBDATE|-13763174|                                               
            LSOWNER|                                                           
            VNTNAME|CN3084 pAAV-hI56i-minBglobin-iCre(R297T)-BGHpA (JT)|       
            VNTAUTHORNAME|Jonathan Ting|                                       
            VNTOAUTHORNAME|Feng Zhang|.
FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /vntifkey=21
                     /label="L-ITR"
     misc_feature    complement(156..954)
                     /created_by="erin.groce"
                     /modified_by="ximena.opitzaraya"
                     /label="eHGT_1426m"
     misc_feature    complement(<156..>954)
                     /label="1426-SYFP2_5_Seq_R.ab1 (concatenated sequence 2)
                     (reversed)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    158..954
                     /Original_Bases="AGGTACCGAGCTCTATGCACTCACAGTGGTTTGGCATGCAT
                     CTGGTGAATTTTTTTTAACGAAAAATTAGTGTTGGTTTCGATGTATGGTAGCATTCTC
                     CCTAACGTAATTTGAATAATTCAGCAAAGCCCCACTACCAGCTGTACTTCTGCAGCCT
                     CTTCCATTCTTTTCAGCATTATAATTTTGGTTAATTTTCAATTTTAGGTCCTACGTCT
                     CTGCAATTTGTGTATGAATAACAGAATAATTTCCCTCTTTTGTTTCGCCTTTCCTGTT
                     CCTGAATCTAAATAAAGATGGCTTTTTAGTATTAAAAGTGGAAGAAAATTACAGGTAA
                     TTATCTTTGACGGTAAAAACGCTGTAATCAGCGGGCTACATGAAAAATTACTCTAATT
                     ATGGCTGCATTTAAGAGAATGGAAAAAAACCTTCTTGTGGATAAAAACCTTAAATTGT
                     CCCCAATGTCTGCTTCAAATTGGATGGCACTGCAGCTGGAGGCTTTGTTCAGAATTGA
                     TCCTGGGGAGCTACGAACCCAAAGTTTCACAGTAGG"
                     /label="AGGTACCGAGCTCTATGCACTCACAGTGGTTTGGCATGCATCTGGTGAAT
                     TTTTTTTAACGAAAAATTAGTGTTGGTTTCGATGTATGGTAGCATTCTCCCTAACGTA
                     ATTTGAATAATTCAGCAAAGCCCCACTACCAGCTGTACTTCTGCAGCCTCTTCCATTC
                     TTTTCAGCATTATAATTTTGGTTAATTTTCAATTTTAGGTCCTACGTCTCTGCAATTT
                     GTGTATGAATAACAGAATAATTTCCCTCTTTTGTTTCGCCTTTCCTGTTCCTGAATCT
                     AAATAAAGATGGCTTTTTAGTATTAAAAGTGGAAGAAAATTACAGGTAATTATCTTTG
                     ACGGTAAAAACGCTGTAATCAGCGGGCTACATGAAAAATTACTCTAATTATGGCTGCA
                     TTTAAGAGAATGGAAAAAAACCTTCTTGTGGATAAAAACCTTAAATTGTCCCCAATGT
                     CTGCTTCAAATTGGATGGCACTGCAGCTGGAGGCTTTGTTCAGAATTGATCCTGGGGA
                     GCTACGAACCCAAAGTTTCACAGTAGG"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    961..1013
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey=21
                     /label="Beta\Globin\minimal\promoter"
     misc_feature    970..975
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey=21
                     /label="TATA\box"
     misc_feature    1002
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey=21
                     /label="transcriptional\start"
     misc_feature    1029
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    1042..2097
                     /vntifkey=21
                     /label="iCre(297T)"
     misc_feature    1042..1068
                     /vntifkey=21
                     /label="nls"
     misc_feature    1954..1956
                     /vntifkey=21
                     /label="R297T"
     misc_feature    2017..2019
                     /vntifkey=21
                     /label="V318"
     misc_feature    2085
                     /vntifkey=21
                     /label="G-->A\silent\mutation\to\remove\XhoI\site"
     misc_feature    2131..2334
                     /vntifkey=21
                     /label="BGH-pA"
     primer          complement(2245..2264)
                     /vntifkey=27
                     /label="BGH-R"
     misc_feature    2357..2497
                     /vntifkey=21
                     /label="R-ITR"
     rep_origin      2589..2895
                     /vntifkey=33
                     /label="f1\Origin"
     misc_feature    3414..4271
                     /vntifkey=21
                     /label="AmpR"
     rep_origin      4422..5089
                     /vntifkey=33
                     /label="pUC\ori"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtattat gctaatatgc cataattaac
      181 ataaattaac atagttcttg tgggacccca gttaattcat gtgagaaaac ataaaatgtc
      241 agggtcgcgg cgaggggccg gtaggggtgg gcggaggttt ctgaggaggg agcttttggg
      301 agcttcgagt caacaataaa ggaccgaggg ttcggagcag gagtggcctc ggtaaatgaa
      361 ttcagatata attcaagctt attagagggc tttttaaaaa aataaaaaat tgattcagtg
      421 tatgaaagca agttaactac ttctgttcac cattgagaga cttacaggtg cttgcctgaa
      481 ctgcaataaa ggactcttat tattgatcag gacttattta tctcttcatt ttggagagcc
      541 tcataaactg ttattacagt ttctctactg actttgaaaa gtttgaagtt tgaaagagac
      601 acctgtgcag ctaacaaagc atgagcacgg ccagaacaga gaaccctgtt atcatgggtc
      661 tatccagtca gaacggccag ctgaggggcc ctgtgaaagc cagtgctggc cctggaggag
      721 ggggcacaca gccacagatg aaccagttga agaacaccaa cacaatcaat aacggcactc
      781 cgcagcaagc ccagagcatg gccgccacga ttaagcatgt ctgatgttca taaaggccag
      841 aagagtttgt tgaagccact gaacttaggt tataaatgat gacctggtga tgactggaaa
      901 gacattaaaa ctccctccaa aggatctaag aatcaaaact ctggatgtga cctcgagctc
      961 gggctgggca taaaagtcag ggcagagcca tctattgctt acatttgctt ctgggatcca
     1021 gatctttcga agctagccac catggtgccc aagaagaaga ggaaagtctc caacctgctg
     1081 actgtgcacc aaaacctgcc tgccctccct gtggatgcca cctctgatga agtcaggaag
     1141 aacctgatgg acatgttcag ggacaggcag gccttctctg aacacacctg gaagatgctc
     1201 ctgtctgtgt gcagatcctg ggctgcctgg tgcaagctga acaacaggaa atggttccct
     1261 gctgaacctg aggatgtgag ggactacctc ctgtacctgc aagccagagg cctggctgtg
     1321 aagaccatcc aacagcacct gggccagctc aacatgctgc acaggagatc tggcctgcct
     1381 cgcccttctg actccaatgc tgtgtccctg gtgatgagga gaatcagaaa ggagaatgtg
     1441 gatgctgggg agagagccaa gcaggccctg gcctttgaac gcactgactt tgaccaagtc
     1501 agatccctga tggagaactc tgacagatgc caggacatca ggaacctggc cttcctgggc
     1561 attgcctaca acaccctgct gcgcattgcc gaaattgcca gaatcagagt gaaggacatc
     1621 tcccgcaccg atggtgggag aatgctgatc cacattggca ggaccaagac cctggtgtcc
     1681 acagctggtg tggagaaggc cctgtccctg ggggttacca agctggtgga gagatggatc
     1741 tctgtgtctg gtgtggctga tgaccccaac aactacctgt tctgccgggt cagaaagaat
     1801 ggtgtggctg ccccttctgc cacctcccaa ctgtccaccc gggccctgga agggatcttt
     1861 gaggccaccc accgcctgat ctatggtgcc aaggatgact ctgggcagag atacctggcc
     1921 tggtctggcc actctgccag agtgggtgct gccaccgaca tggccagggc tggtgtgtcc
     1981 atccctgaaa tcatgcaggc tggtggctgg accaatgtga acattgtgat gaactacatc
     2041 agaaacctgg actctgagac tggggccatg gtgaggctgc tcgaagatgg ggactgaggc
     2101 gcgccgaatt caagcttctc gagagatctt cgactgtgcc ttctagttgc cagccatctg
     2161 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     2221 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     2281 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgcacgtg
     2341 cggaccgagc ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct
     2401 cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg
     2461 gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc
     2521 cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct
     2581 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     2641 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     2701 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     2761 ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct
     2821 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     2881 tccaaactgg aacaacactc aaccctatct cgggctattc ttttgattta taagggattt
     2941 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     3001 ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg
     3061 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     3121 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     3181 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     3241 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     3301 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     3361 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     3421 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     3481 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     3541 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     3601 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     3661 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     3721 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     3781 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     3841 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     3901 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     3961 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     4021 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     4081 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     4141 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     4201 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     4261 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     4321 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     4381 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     4441 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     4501 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     4561 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     4621 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     4681 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     4741 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     4801 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     4861 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     4921 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     4981 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     5041 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgt
//
