LOCUS       CN3044_pAAV-3xco        4782 bp DNA     circular SYN 17-NOV-2024
DEFINITION  synthetic circular DNA
ACCESSION   urn.local...b8-cq6a6ml
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 17, 2024 from SnapGene 7.2.1
            https://www.snapgene.com
COMMENT     ##Comment-START## Comment :: rAAV-ESYN-SYFP2-WPRE3-BGHpA            
            clone #8-1 correct , 8-2 backup ##Comment-END## 
            ##Imported_From-START## Path     :: C:\VNTI Database Filename :: 
            _exdata.ma4 ##Imported_From-END##
FEATURES             Location/Qualifiers
     source          1..4782
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..141
                     /label=L-ITR
     misc_feature    156..737
                     /label=3xcore2_eHGT_779m
                     /note="/created_by=ximena.opitzaraya"
     misc_feature    157..737
                     /label=TAGGTACCGAGCTCTATGCACTCACAGTGGTTTGGCATGCATCTGGTGAA 
                     TTTTTTTTAACGAAAAATTAGTGTTGGTTTCGATGTATGGTAGCATTCTCCCTAACGT 
                     AATTTGAATAATTCAGCAAAGCCCCACTACCAGCTGTACTTCTGCAGCCTCTTCCATT 
                     CTTTTCAGCATTATAATTTTGGTTAATTTTCAATTTTAGGTCCTACGTCTCTGCAATT 
                     TGTGTATGAATAACAGAATAATTTCCCTCTTTTGTTTCGCCTTTCCTGTTCCTGAATC 
                     TAAATAAAGATGGCTTTTTAGTATTAAAAGTGGAAGAAAATTACAGGTAATTATCTTT 
                     GACGGTAAAAACGCTGTAATCAGCGGGCTACATGAAAAATTACTCTAATTATGGCTGC 
                     ATTTAAGAGAATGGAAAAAAACCTTCTTGTGGATAAAAACCTTAAATTGTCCCCAATG 
                     TCTGCTTCAAATTGGATGGCACTGCAGCTGGAGGCTTTGTTCAGAATTGATCCTGGGG 
                     AGCTACGAACCCAAAGTTTCACAGTAGG
                     /label=nonstandard type: Editing History Replacement
                     /note="/Original_Bases=TAGGTACCGAGCTCTATGCACTCACAGTGGTTTGGC
                     ATGCA 
                     TCTGGTGAATTTTTTTTAACGAAAAATTAGTGTTGGTTTCGATGTATGGTAGCATTCT 
                     CCCTAACGTAATTTGAATAATTCAGCAAAGCCCCACTACCAGCTGTACTTCTGCAGCC 
                     TCTTCCATTCTTTTCAGCATTATAATTTTGGTTAATTTTCAATTTTAGGTCCTACGTC 
                     TCTGCAATTTGTGTATGAATAACAGAATAATTTCCCTCTTTTGTTTCGCCTTTCCTGT 
                     TCCTGAATCTAAATAAAGATGGCTTTTTAGTATTAAAAGTGGAAGAAAATTACAGGTA 
                     ATTATCTTTGACGGTAAAAACGCTGTAATCAGCGGGCTACATGAAAAATTACTCTAAT 
                     TATGGCTGCATTTAAGAGAATGGAAAAAAACCTTCTTGTGGATAAAAACCTTAAATTG 
                     TCCCCAATGTCTGCTTCAAATTGGATGGCACTGCAGCTGGAGGCTTTGTTCAGAATTG 
                     ATCCTGGGGAGCTACGAACCCAAAGTTTCACAGTAGG"
     misc_feature    744..796
                     /label=Beta Globin minimal promoter
     misc_feature    753..758
                     /label=TATA box
     misc_feature    785
                     /label=transcriptional start
     misc_feature    821..1531
                     /label=CN1244_pAAV Linearized (digested by SacI, MluI)
                     Fragment 3 (concatenated sequence 1)
                     /label=nonstandard type: Concatenated sequence
                     /note="/Source=referenceToDocument: 
                     urn:local:.:12i-fwwg6gs"
     misc_feature    821..1514
                     /label=CN1244_pAAV Linearized (digested by SacI, MluI)
                     Fragment 3 (concatenated sequence 1)
                     /label=nonstandard type: Concatenated sequence
                     /note="/Source=referenceToDocument: 
                     urn:local:.:3sf-fn0ztcm"
     misc_feature    823..1500
                     /label=TACCGGTCGCCACCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTG 
                     GTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCG 
                     GCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGCTGATCTGCACCAC 
                     CGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGGGCTACGGCGTGCAG 
                     TGCTTCGCCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGC 
                     CCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGAC 
                     CCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGC 
                     ATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACA 
                     GCCACAACGTCTATATCACCGCCGACAAGCAGAAGAACGGCATCAAGGCCAACTTCAA 
                     GATCCGCCACAACATCGAGGACGGCGGCGTGCAGCTCGCCGACCACTACCAGCAGAAC 
                     ACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCTACCAGT 
                     CCAAGCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGT 
                     GACCGCCGCCGGGATCACTCT
                     /label=nonstandard type: Editing History Replacement
                     /note="/Original_Bases=TACCGGTCGCCACCATGGTGAGCAAGGGCGAGGAGC
                     TGTTC 
                     ACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCA 
                     GCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGCTGAT 
                     CTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGGGCTAC 
                     GGCGTGCAGTGCTTCGCCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGT 
                     CCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAA 
                     CTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAG 
                     CTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACA 
                     ACTACAACAGCCACAACGTCTATATCACCGCCGACAAGCAGAAGAACGGCATCAAGGC 
                     CAACTTCAAGATCCGCCACAACATCGAGGACGGCGGCGTGCAGCTCGCCGACCACTAC 
                     CAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGA 
                     GCTACCAGTCCAAGCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCT 
                     GGAGTTCGTGACCGCCGCCGGGATCACTCT"
     gene            827..1525
                     /label=mScarlet
                     /note="/Color=#ff0084"
                     /note="/modified_by=erikh"
                     /note="/modified_by=refugio.martinez"
                     /note="/modified_by=ximena.opitzaraya"
     misc_feature    1560..1805
                     /label=WPRE3
     misc_feature    1819..2022
                     /label=BGH-pA
     primer_bind     complement(1933..1952)
                     /label=BGH-R
     misc_feature    2045..2185
                     /label=R-ITR
     rep_origin      2277..2583
                     /label=f1 Origin
     misc_feature    3102..3959
                     /label=AmpR
     rep_origin      4110..4777
                     /label=pUC ori
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtaagtt tgagacaaat taaggaaaag
      181 cgggttggga ggagtctgag gctgtgggtg gctgcctcga gaggctgctc acaacctgac
      241 atgtttcaga tcttttgaca cggtcatgca agcaacagaa acttatatgg tttttagtct
      301 cccaggtagc agggaaatga tgatgtggga ggattagggg ggggaaaata agtttgagac
      361 aaattaagga aaagcgggtt gggaggagtc tgaggctgtg ggtggctgcc tcgagaggct
      421 gctcacaacc tgacatgttt cagatctttt gacacggtca tgcaagcaac agaaacttat
      481 atggttttta gtctcccagg tagcagggaa atgatgatgt gggaggatta ggggggggaa
      541 aataagtttg agacaaatta aggaaaagcg ggttgggagg agtctgaggc tgtgggtggc
      601 tgcctcgaga ggctgctcac aacctgacat gtttcagatc ttttgacacg gtcatgcaag
      661 caacagaaac ttatatggtt tttagtctcc caggtagcag ggaaatgatg atgtgggagg
      721 attagggggg ggaaaatgag ctcgggctgg gcataaaagt cagggcagag ccatctattg
      781 cttacatttg cttctgggat ccagatcttt cgaagctagc gccaccatgg tgagcaaggg
      841 cgaggcagtg atcaaggagt tcatgcggtt caaggtgcac atggagggct ccatgaacgg
      901 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
      961 caagctgaag gtgaccaagg gtggccccct gcccttctcc tgggacatcc tgtcccctca
     1021 gttcatgtac ggctccaggg ccttcaccaa gcaccccgcc gacatccccg actactataa
     1081 gcagtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgc
     1141 cgtgaccgtg acccaggaca cctccctgga ggacggcacc ctgatctaca aggtgaagct
     1201 ccgcggcacc aacttccctc ctgacggccc cgtaatgcag aagaagacaa tgggctggga
     1261 agcgtccacc gagcggttgt accccgagga cggcgtgctg aagggcgaca ttaagatggc
     1321 cctgcgcctg aaggacggcg gccgctacct ggcggacttc aagaccacct acaaggccaa
     1381 gaagcccgtg cagatgcccg gcgcctacaa cgtcgaccgc aagttggaca tcacctccca
     1441 caacgaggac tacaccgtgg tggaacagta cgaacgctcc gagggccgcc actccaccgg
     1501 cggcatggac gagctgtaca agtaagtcga cggcgcgccg cggccgcgaa ttcgatatca
     1561 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     1621 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     1681 tatggctttc attttctcct ccttgtataa atcctggtta gttcttgcca cggcggaact
     1741 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     1801 cgtggctcga gagatcttcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     1861 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     1921 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     1981 aggacagcaa gggggaggat tgggaagaca atagcaggca tgcacgtgcg gaccgagcgg
     2041 ccgcaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact
     2101 gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc
     2161 gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt attttctcct tacgcatctg
     2221 tgcggtattt cacaccgcat acgtcaaagc aaccatagta cgcgccctgt agcggcgcat
     2281 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     2341 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     2401 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     2461 ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     2521 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     2581 caacactcaa ccctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg
     2641 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     2701 taacgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     2761 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     2821 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     2881 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
     2941 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     3001 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     3061 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     3121 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     3181 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     3241 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     3301 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     3361 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     3421 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     3481 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     3541 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     3601 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     3661 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     3721 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     3781 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     3841 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     3901 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     3961 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     4021 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     4081 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     4141 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     4201 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     4261 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     4321 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     4381 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     4441 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     4501 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     4561 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     4621 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     4681 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     4741 ttttacggtt cctggccttt tgctggcctt ttgctcacat gt
//
