LOCUS       CN5646_pAAV-AiE1426m_3xC2-minBG-SYFP2-WPRE3-BGHpA        5128 bp    DNA     linear   UNA 02-MAY-2024
DEFINITION  .
ACCESSION   urn.local...4bu-hlx0q74
VERSION     urn.local...4bu-hlx0q74
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /label="L-ITR"
     misc_feature    156..454
                     /created_by="ximena.opitzaraya"
                     /label="eHGT_1426m_c2"
     misc_feature    157..1051
                     /Original_Bases="TAGGTACCGAGCTCTATGCACTCACAGTGGTTTGGCATGCA
                     TCTGGTGAATTTTTTTTAACGAAAAATTAGTGTTGGTTTCGATGTATGGTAGCATTCT
                     CCCTAACGTAATTTGAATAATTCAGCAAAGCCCCACTACCAGCTGTACTTCTGCAGCC
                     TCTTCCATTCTTTTCAGCATTATAATTTTGGTTAATTTTCAATTTTAGGTCCTACGTC
                     TCTGCAATTTGTGTATGAATAACAGAATAATTTCCCTCTTTTGTTTCGCCTTTCCTGT
                     TCCTGAATCTAAATAAAGATGGCTTTTTAGTATTAAAAGTGGAAGAAAATTACAGGTA
                     ATTATCTTTGACGGTAAAAACGCTGTAATCAGCGGGCTACATGAAAAATTACTCTAAT
                     TATGGCTGCATTTAAGAGAATGGAAAAAAACCTTCTTGTGGATAAAAACCTTAAATTG
                     TCCCCAATGTCTGCTTCAAATTGGATGGCACTGCAGCTGGAGGCTTTGTTCAGAATTG
                     ATCCTGGGGAGCTACGAACCCAAAGTTTCACAGTAG"
                     /label="TAGGTACCGAGCTCTATGCACTCACAGTGGTTTGGCATGCATCTGGTGAA
                     TTTTTTTTAACGAAAAATTAGTGTTGGTTTCGATGTATGGTAGCATTCTCCCTAACGT
                     AATTTGAATAATTCAGCAAAGCCCCACTACCAGCTGTACTTCTGCAGCCTCTTCCATT
                     CTTTTCAGCATTATAATTTTGGTTAATTTTCAATTTTAGGTCCTACGTCTCTGCAATT
                     TGTGTATGAATAACAGAATAATTTCCCTCTTTTGTTTCGCCTTTCCTGTTCCTGAATC
                     TAAATAAAGATGGCTTTTTAGTATTAAAAGTGGAAGAAAATTACAGGTAATTATCTTT
                     GACGGTAAAAACGCTGTAATCAGCGGGCTACATGAAAAATTACTCTAATTATGGCTGC
                     ATTTAAGAGAATGGAAAAAAACCTTCTTGTGGATAAAAACCTTAAATTGTCCCCAATG
                     TCTGCTTCAAATTGGATGGCACTGCAGCTGGAGGCTTTGTTCAGAATTGATCCTGGGG
                     AGCTACGAACCCAAAGTTTCACAGTAG"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    455..753
                     /created_by="ximena.opitzaraya"
                     /label="eHGT_1426m_c2"
     misc_feature    754..1052
                     /created_by="ximena.opitzaraya"
                     /label="eHGT_1426m_c2"
     misc_feature    1059..1111
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Beta Globin minimal promoter"
     misc_feature    1068..1073
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="TATA box"
     misc_feature    1100
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="transcriptional start"
     misc_feature    1152..1871
                     /label="SYFP2"
     primer_bind     1198..1218
                     /label="EGFP-N-R"
     misc_feature    1906..2151
                     /label="WPRE3"
     misc_feature    2165..2368
                     /label="BGH-pA"
     primer          complement(2279..2298)
                     /label="BGH-R"
     misc_feature    2391..2531
                     /label="R-ITR"
     rep_origin      2623..2929
                     /label="f1 Origin"
     misc_feature    3448..4305
                     /label="AmpR"
     rep_origin      4456..5123
                     /label="pUC ori"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtaaaaa ttgattcagt gtatgaaagc
      181 aagttaacta cttctgttca ccattgagag acttacaggt gcttgcctga actgcaataa
      241 aggactctta ttattgatca ggacttattt atctcttcat tttggagagc ctcataaact
      301 gttattacag tttctctact gactttgaaa agtttgaagt ttgaaagaga cacctgtgca
      361 gctaacaaag catgagcacg gccagaacag agaaccctgt tatcatgggt ctatccagtc
      421 agaacggcca gctgaggggc cctgtgaaag ccagaaaaat tgattcagtg tatgaaagca
      481 agttaactac ttctgttcac cattgagaga cttacaggtg cttgcctgaa ctgcaataaa
      541 ggactcttat tattgatcag gacttattta tctcttcatt ttggagagcc tcataaactg
      601 ttattacagt ttctctactg actttgaaaa gtttgaagtt tgaaagagac acctgtgcag
      661 ctaacaaagc atgagcacgg ccagaacaga gaaccctgtt atcatgggtc tatccagtca
      721 gaacggccag ctgaggggcc ctgtgaaagc cagaaaaatt gattcagtgt atgaaagcaa
      781 gttaactact tctgttcacc attgagagac ttacaggtgc ttgcctgaac tgcaataaag
      841 gactcttatt attgatcagg acttatttat ctcttcattt tggagagcct cataaactgt
      901 tattacagtt tctctactga ctttgaaaag tttgaagttt gaaagagaca cctgtgcagc
      961 taacaaagca tgagcacggc cagaacagag aaccctgtta tcatgggtct atccagtcag
     1021 aacggccagc tgaggggccc tgtgaaagcc aggagctcgg gctgggcata aaagtcaggg
     1081 cagagccatc tattgcttac atttgcttct gggatccaga tctttcgaag ctagcgctac
     1141 cggtcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     1201 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     1261 atgccaccta cggcaagctg accctgaagc tgatctgcac caccggcaag ctgcccgtgc
     1321 cctggcccac cctcgtgacc accctgggct acggcgtgca gtgcttcgcc cgctaccccg
     1381 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     1441 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     1501 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     1561 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc accgccgaca
     1621 agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag gacggcggcg
     1681 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     1741 ccgacaacca ctacctgagc taccagtcca agctgagcaa agaccccaac gagaagcgcg
     1801 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     1861 tgtacaagta agtcgacggc gcgccgcggc cgcgaattcg atatcataat caacctctgg
     1921 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     1981 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     2041 tctcctcctt gtataaatcc tggttagttc ttgccacggc ggaactcatc gccgcctgcc
     2101 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gctcgagaga
     2161 tcttcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     2221 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     2281 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     2341 gaggattggg aagacaatag caggcatgca cgtgcggacc gagcggccgc aggaacccct
     2401 agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc
     2461 aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag
     2521 ctgcctgcag gggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     2581 ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt
     2641 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     2701 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     2761 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     2821 ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     2881 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     2941 atctcgggct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     3001 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt
     3061 ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     3121 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     3181 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     3241 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     3301 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     3361 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     3421 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     3481 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     3541 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     3601 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     3661 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     3721 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     3781 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     3841 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     3901 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     3961 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     4021 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     4081 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     4141 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     4201 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     4261 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     4321 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     4381 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     4441 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     4501 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     4561 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     4621 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     4681 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     4741 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     4801 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     4861 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     4921 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     4981 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     5041 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     5101 gccttttgct ggccttttgc tcacatgt
//
