LOCUS       Exported                8535 bp ds-DNA     circular SYN 15-NOV-2024
DEFINITION  521.
ACCESSION   521
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8535)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Nov 18, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     source          1..8535
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1..546
                     /label=p15A ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     rep_origin      634..689
                     /label=RSF ori (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=7.5"
                     /note="/other=rep_origin"
     terminator      complement(708..735)
                     /label=T7Te terminator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
     terminator      complement(751..822)
                     /label=rrnB T1 terminator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
     CDS             complement(848..4952)
                     /label=dCas9 (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     protein_bind    complement(4985..5008)
                     /label=tetracycline response element (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.9"
                     /note="/other=protein_bind"
     promoter        complement(5015..5069)
                     /label=tetR/tetA promoters
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=98.2"
                     /note="/match_length=98.2"
                     /note="/other=promoter"
     CDS             5088..5711
                     /label=TetR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     misc_feature    5727..5858
                     /label=crRNA leader
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     repeat_region   5859..5894
                     /label=DR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=repeat_region"
     repeat_region   5925..5960
                     /label=DR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=repeat_region"
     misc_RNA        6081..6159
                     /label=tracrRNA
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_RNA"
     CDS             6350..6469
                     /label=tnpA (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=86.0"
                     /note="/match_length=8.4"
                     /note="/other=CDS"
     CDS             6501..7292
                     /label=neo
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     terminator      7314..7408
                     /label=lambda t0 terminator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
     terminator      complement(7501..7528)
                     /label=T7Te terminator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=96.4"
                     /note="/match_length=100.0"
                     /note="/other=terminator"
     CDS             complement(7623..8339)
                     /label=mCerulean
                     /note="pLannotate"
                     /note="/database=fpbase"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(8361..8395)
                     /label=J23119(SpeI) promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     terminator      8413..8429
                     /label=bacterial terminator (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=38.6"
                     /note="/other=terminator"
ORIGIN
        1 ttgagatcgt tttggtctgc gcgtaatctc ttgctctgaa aacgaaaaaa ccgccttgca
       61 gggcggtttt tcgaaggttc tctgagctac caactctttg aaccgaggta actggcttgg
      121 aggagcgcag tcaccaaaac ttgtcctttc agtttagcct taaccggcgc atgacttcaa
      181 gactaactcc tctaaatcaa ttaccagtgg ctgctgccag tggtgctttt gcatgtcttt
      241 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcggac tgaacggggg
      301 gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc ggaactgagt gtcaggcgtg
      361 gaatgagaca aacgcggcca taacagcgga atgacaccgg taaaccgaaa ggcaggaaca
      421 ggagagcgca cgagggagcc gccaggggga aacgcctggt atctttatag tcctgtcggg
      481 tttcgccacc actgatttga gcgtcagatt tcgtgatgct tgtcaggggg gcggagccta
      541 tggaaaaacg gctttgccgc ggccctctca cttccctgtt aagtatcttc ctggcatctt
      601 ccaggaaatc tccgccccgt tcgtaagcca tttccgctcg ccgcagtcga acgaccgagc
      661 gtagcgagtc agtgagcgag gaagcggaat atatccctag gtataaacgc agaaaggccc
      721 acccgaaggt gagccagtgt gactctagta gagagcgttc accgacaaac aacagataaa
      781 acgaaaggcc cagtctttcg actgagcctt tcgttttatt tgatgcctgg agatccttac
      841 tcgagttagt cacctcctag ctgactcaaa tcaatgcgtg tttcataaag accagtgatg
      901 gattgatgga taagagtggc atctaaaact tcttttgtag acgtatatcg tttacgatca
      961 attgttgtat caaaatattt aaaagcagcg ggagctccaa gattcgtcaa cgtaaataaa
     1021 tgaataatat tttctgcttg ttcacgtatt ggtttgtctc tatgtttgtt atatgcacta
     1081 agaactttat ctaaattggc atctgctaaa ataacacgct tagaaaattc actgatttgc
     1141 tcaataatct catctaaata atgcttatgc tgctccacaa acaattgttt ttgttcgtta
     1201 tcttctggac tacccttcaa cttttcataa tgactagcta aatataaaaa attcacatat
     1261 ttgcttggca gagccagctc atttcctttt tgtaattctc cggcactagc cagcatccgt
     1321 ttacgaccgt tttctaactc aaaaagacta tatttaggta gtttaatgat taagtctttt
     1381 ttaacttcct tatatccttt agcttctaaa aagtcaatcg gatttttttc aaaggaactt
     1441 ctttccataa ttgtgatccc tagtaactct ttaacggatt ttaacttctt cgatttccct
     1501 ttttccacct tagcaaccac taggactgaa taagctaccg ttggactatc aaaaccacca
     1561 tatttttttg gatcccagtc ttttttacga gcaataagct tgtccgaatt tctttttggt
     1621 aaaattgact ccttggagaa tccgcctgtc tgtacttctg ttttcttgac aatattgact
     1681 tggggcatgg acaatacttt gcgcactgtg gcaaaatctc gccctttatc ccagacaatt
     1741 tctccagttt ccccattagt ttcgattaga gggcgtttgc gaatctctcc atttgcaagt
     1801 gtaatttctg ttttgaagaa gttcatgata ttagagtaaa agaaatattt tgcggttgct
     1861 ttgcctattt cttgctcaga cttagcaatc attttacgaa catcataaac tttataatca
     1921 ccatagacaa actccgattc aagttttgga tatttcttaa tcaaagcagt tccaacgacg
     1981 gcatttagat acgcatcatg ggcatgatgg taattgttaa tctcacgtac tttatagaat
     2041 tggaaatctt ttcggaagtc agaaactaat ttagatttta aggtaatcac tttaacctct
     2101 cgaataagtt tatcattttc atcgtattta gtattcatgc gactatccaa aatttgtgcc
     2161 acatgcttag tgatttggcg agtttcaacc aattggcgtt tgataaaacc agctttatca
     2221 agttcactca aacctccacg ttcagctttc gttaaattat caaacttacg ttgagtgatt
     2281 aacttggcgt ttagaagttg tctccaatag tttttcatct ttttgactac ttcttcactt
     2341 ggaacgttat ccgatttacc acgattttta tcagaacgcg ttaagacctt attgtctatt
     2401 gaatcgtctt taaggaaact ttgtggaaca atggcatcga catcataatc acttaaacga
     2461 ttaatatcta attcttggtc cacatacatg tctcttccat tttggagata atagagatag
     2521 agcttttcat tttgcaattg agtattttca acaggatgct ctttaagaat ctgacttcct
     2581 aattctttga taccttcttc gattcgtttc atacgctctc gcgaattttt ctggcccttt
     2641 tgagttgtct gattttcacg tgccatttca ataacgatat tttctggctt atgccgcccc
     2701 attactttga ccaattcatc aacaactttt acagtctgta aaataccttt tttaatagca
     2761 gggctaccag ctaaatttgc aatatgttca tgtaaactat cgccttgtcc agacacttgt
     2821 gctttttgaa tgtcttcttt aaatgtcaaa ctatcatcat ggatcagctg cataaaattg
     2881 cgattggcaa aaccatctga tttcaaaaaa tctaatattg ttttgccaga ttgcttatcc
     2941 ctaataccat taatcaattt tcgagacaaa cgtccccaac cagtataacg gcgacgttta
     3001 agctgtttca tcaccttatc atcaaagagg tgagcatatg ttttaagtct ttcctcaatc
     3061 atctccctat cttcaaataa ggtcaatgtt aaaacaatat cctctaagat atcttcattt
     3121 tcttcattat ccaaaaaatc tttatcttta ataattttta gcaaatcatg gtaggtacct
     3181 aatgaagcat taaatctatc ttcaactcct gaaatttcaa cactatcaaa acattctatt
     3241 tttttgaaat aatcttcttt taattgctta acggttactt ttcgatttgt tttgaagagt
     3301 aaatcaacaa tggctttctt ctgttcacct gaaagaaatg ctggttttcg cattccttca
     3361 gtaacatatt tgacctttgt caattcgtta taaaccgtaa aatactcata aagcaaacta
     3421 tgttttggta gtactttttc atttggaaga tttttatcaa agtttgtcat gcgttcaata
     3481 aatgattgag ctgaagcacc tttatcgaca acttcttcaa aattccatgg ggtaattgtt
     3541 tcttcagact tccgagtcat ccatgcaaaa cgactattgc cacgcgccaa tggaccaaca
     3601 taataaggaa ttcgaaaagt caagattttt tcaatcttct cacgattgtc ttttaaaaat
     3661 ggataaaagt cttcttgtct tctcaaaata gcatgcagct cacccaagtg aatttgatgg
     3721 ggaatagagc cgttgtcaaa ggtccgttgc ttgcgcagca aatcttcacg atttagtttc
     3781 accaataatt cctcagtacc atccattttt tctaaaattg gtttgataaa tttataaaat
     3841 tcttcttggc tagctccccc atcaatataa cctgcatatc cgttttttga ttgatcaaaa
     3901 aagatttctt tatacttttc tggaagttgt tgtcgaacta aagcttttaa aagagtcaag
     3961 tcttgatgat gttcatcgta gcgtttaatc attgaagctg ataggggagc cttagttatt
     4021 tcagtattta ctcttaggat atctgaaagt aaaatagcat ctgataaatt cttagctgcc
     4081 aaaaacaaat cagcatattg atctccaatt tgcgccaata aattatctaa atcatcatcg
     4141 taagtatctt ttgaaagctg taatttagca tcttctgcca aatcaaaatt tgatttaaaa
     4201 ttaggggtca aacccaatga caaagcaatg agattcccaa ataagccatt tttcttctca
     4261 ccggggagct gagcaatgag attttctaat cgtcttgatt tactcaatcg tgcagaaaga
     4321 atcgctttag catctactcc acttgcgtta atagggtttt cttcaaataa ttgattgtag
     4381 gtttgtacca actggataaa tagtttgtcc acatcactat tatcaggatt taaatctccc
     4441 tcaatcaaaa aatgaccacg aaacttaatc atatgcgcta aggccaaata gattaagcgc
     4501 aaatccgctt tatcagtaga atctaccaat ttttttcgca gatgatagat agttggatat
     4561 ttctcatgat aagcaacttc atctactata tttccaaaaa taggatgacg ttcatgcttc
     4621 ttgtcttctt ccaccaaaaa agactcttca agtcgatgaa agaaactatc atctactttc
     4681 gccatctcat ttgaaaaaat ctcctgtaga taacaaatac gattcttccg acgtgtatac
     4741 cttctacgag ctgtccgttt gagacgagtc gcttccgctg tctctccact gtcaaataaa
     4801 agagccccta taagattttt tttgatactg tggcggtctg tatttcccag aaccttgaac
     4861 tttttagacg gaaccttata ttcatcagtg atcaccgccc atccgacgct atttgtgccg
     4921 atagctaagc ctattgagta tttcttatcc atagatcctt tctcctcttt agatcttttg
     4981 aattcttttc tctatcactg atagggaggg tcaatctcta tcactgatag ggagtggtaa
     5041 aataactcta tcaacgatag agtgtcaaca aaaattagga attaatgatg tctagattag
     5101 ataaaagtaa agtgattaac agcgcattag agctgcttaa tgaggtcgga atcgaaggtt
     5161 taacaacccg taaactcgcc cagaagctag gtgtagagca gcctacattg tattggcatg
     5221 taaaaaataa gcgggctttg ctcgacgcct tagccattga gatgttagat aggcaccata
     5281 ctcacttttg ccctttagaa ggggaaagct ggcaagattt tttacgtaat aacgctaaaa
     5341 gttttagatg tgctttacta agtcatcgcg atggagcaaa agtacattta ggtacacggc
     5401 ctacagaaaa acagtatgaa actctcgaaa atcaattagc ctttttatgc caacaaggtt
     5461 tttcactaga gaatgcatta tatgcactca gcgctgtggg gcattttact ttaggttgcg
     5521 tattggaaga tcaagagcat caagtcgcta aagaagaaag ggaaacacct actactgata
     5581 gtatgccgcc attattacga caagctatcg aattatttga tcaccaaggt gcagagccag
     5641 ccttcttatt cggccttgaa ttgatcatat gcggattaga aaaacaactt aaatgtgaaa
     5701 gtgggtctta aggtaccatg ggatcctatt tcttaataac taaaaatatg gtataatact
     5761 cttaataaat gcagtaatac aggggctttt caagactgaa gtctagctga gacaaatagt
     5821 gcgattacga aattttttag acaaaaatag tctacgaggt tttagagcta tgctgttttg
     5881 aatggtccca aaacgctgtg tgtagtaata aatcagccct aaatgtttta gagctatgct
     5941 gttttgaatg gtcccaaaac ttcagcacac tgagacttgt tgagttgaat tcatgagtat
     6001 taagtattgt tttatggctg ataaatttct ttgaatttct ccttgattat ttgttataaa
     6061 agttataaaa taatcttgtt ggaaccattc aaaacagcat agcaagttaa aataaggcta
     6121 gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttgata cttctattct
     6181 actctgactg caaaccaaaa aaacaagcgc tttcaaaacg cttgttttat catttttagg
     6241 gaaattaatc tcttaatcct tttatcattc tacatttagg cgctgccatc ttaagacgaa
     6301 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     6361 cgtcggaatt gccagctggg gcgccctctg gtaaggttgg gaagccctgc aaagtaaact
     6421 ggatggcttt cttgccgcca aggatctgat ggcgcagggg atcaagatct gatcaagaga
     6481 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
     6541 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     6601 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     6661 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg
     6721 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     6781 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     6841 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     6901 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     6961 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     7021 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
     7081 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     7141 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     7201 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     7261 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctctcgc ttggactcct
     7321 gttgatagat ccagtaatga cctcagaact ccatctggat ttgttcagaa cgctcggttg
     7381 ccgccgggcg ttttttattg gtgagaatcc aagcactagt tggtaccggc atcaatagta
     7441 aaataataaa aaagccggat taataatctg gctttttata ttctctctct agtatataaa
     7501 cgcagaaagg ccaacccgaa ggtgagccag tgtgagacta tcatactcat atcgtgtgaa
     7561 gatttgctgc gctattcaga tcctcttctg agatgagttt ttgttcgggc ccaagcttct
     7621 cacttataca attcatccat gccgagggta ataccggccg ctgtcacaaa ctccaggaga
     7681 accatatggt cacgtttttc gtttgggtct ttagagagtt ttgactgcgt cgacaggtaa
     7741 tggttgtcag ggagcagtac cggcccatcg ccgatcgggg tattctgctg gtagtgatcc
     7801 gccagttgca cgctcccatc ttcgatattg tggcgaattt taaagtttgc cttgatgcca
     7861 tttttctgtt tatctgcagt gatataaaca ttatcgctaa tagcgttgta ttccagcttg
     7921 tgcccaagaa tgttgccgtc ttctttgaaa tcaattcctt tcagttcgat gcgatttacc
     7981 agcgtatcgc cctcaaattt cacttccgca cgggttttgt agttcccatc gtccttaaag
     8041 aaaattgtgc gttcctgaac ataaccttcc ggcattgcag atttaaagaa gtcgtgttgt
     8101 ttcatatgat ctgggtaacg cgcaaaacac tgtacccccc acgttaatgt cgtgacaagg
     8161 gtaggccagg gcaccggcag tttgccagtc gtacagatga atttgagcgt gagcttgccg
     8221 tacgtcgcgt caccctcgcc ctcgccgctg accgaaaatt tatggccatt cacatcacca
     8281 tcaagttcaa caagaatcgg caccacgccg gtaaataatt cttcgccctt gcttaccatt
     8341 tagtatttct cctctttagt actagtatta tacctaggac tgagctagct gtcaaagatc
     8401 tttagaattc cagaaatcat ccttagcgac tagtaacaac ttatatcgta tggggctgac
     8461 ttcaggtgct acatttgaag agataaattg cactgaaatc tagaaatatt ttatctgatt
     8521 aataagatga tcttc
//
