LOCUS       pCS2_FLAG-Fem1B_L597A   6028 bp ds-DNA     circular     20-NOV-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    2157..2190
                     /label="CMV fwd"
     misc_feature    2266..2283
                     /label="SP6_Fwd"
     misc_feature    2311..2313
                     /label="SP6"
     misc_feature    2314..2379
                     /label="3X FLAG"
     CDS             2314..2379
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             2377..2397
                     /translation="KGGGVDM"
     misc_feature    2379..2396
                     /label="linker?"
     CDS             2379..2396
                     /translation="GRRSRH"
     CDS             2395..3426
                     /label="Translation 2395-3426"
                     /translation="MEGLAGYVYKAASEGKVLTLAALLLNRSESDIRYLLGYVSQQGGQRSTPLIIAARNGHAKVVRLLLEHYRVQTQQTGTVRFDGYVIDGATALWCAAGAGHFEVVKLLVSHGANVNHTTVTNSTPLRAACFDGRLDIVKYLVENNANISIANKYDNTCLMIAAYKGHTDVVRYLLEQRADPNAKAHCGATALHFAAEAGHIDIVKELIKWRAAIVVNGHGMTPLKVAAESCKADVVELLLSHADCDRRSRIEALELLGASFANDRENYDIMKTYHYLYLAMLERFQDGDNILEKEVLPPIHAYGNRTECRNPQELEAIRQDRDALHMEGLIVRERILGADNIDVS"
     CDS             2395..4278
                     /translation="MEGLAGYVYKAASEGKVLTLAALLLNRSESDIRYLLGYVSQQGGQRSTPLIIAARNGHAKVVRLLLEHYRVQTQQTGTVRFDGYVIDGATALWCAAGAGHFEVVKLLVSHGANVNHTTVTNSTPLRAACFDGRLDIVKYLVENNANISIANKYDNTCLMIAAYKGHTDVVRYLLEQRADPNAKAHCGATALHFAAEAGHIDIVKELIKWRAAIVVNGHGMTPLKVAAESCKADVVELLLSHADCDRRSRIEALELLGASFANDRENYDIMKTYHYLYLAMLERFQDGDNILEKEVLPPIHAYGNRTECRNPQELEAIRQDRDALHMEGLIVRERILGADNIDVSHPIIYRGAVYADNMEFEQCIKLWLHALHLRQKGNRNTHKDLLRFAQVFSQMIHLNEAVKAPDIECVLRCSVLEIEQSMNRVKNISDADVHSAMDNYECNLYTFLYLVCISTKTQCSEEDQCRINKQIYNLIHLDPRTREGFSLLHLAVNSNTPVDDFHTNDVCSFPNALVTKLLLDCGAEVNAVDNEGNSALHIIVQYNRPISDFLTLHSIIISLVEAGAHTDMTNKQNKTPLDKSTTGVSEILLKTQMKMSAKCLAARAVRANDINYQDQIPRTLEEFVGFH*"
     misc_feature    2397..2642
                     /label="Exon 1"
     misc_feature    2621..2678
                     /label="I86"
     misc_feature    2643..4278
                     /label="exon 2"
     misc_feature    2645..2665
                     /label="exon 2 fwd"
     misc_feature    2704..2723
                     /label="gRNA(56) 3:1 0.92"
     misc_feature    2715..2732
                     /label="Fem1B_R126_seq_F"
     misc_feature    2732..2751
                     /label="Exon2-1"
     misc_feature    complement(2732..2751)
                     /label="IDT3*"
     misc_feature    2770..2772
                     /label="R126Q"
     misc_feature    complement(2851..2871)
                     /label="Fem1B_R126_seq_R"
     misc_feature    2861..2911
                     /label="K164"
     misc_feature    2950..2952
                     /label="C186S"
     misc_feature    2995..3020
                     /label="oAGM 475"
     misc_feature    3048..3067
                     /label="exon2-2"
     misc_feature    complement(3048..3067)
                     /label="***IDT5"
     misc_feature    3119..3138
                     /label="gRNA exon 2-1 (56) CHOP#2"
     misc_feature    complement(3130..3151)
                     /label="exon 2 rev"
     misc_feature    complement(3132..3151)
                     /label="IDT4"
     misc_feature    3424..3525
                     /label="TPR"
     CDS             3427..4278
                     /label="Translation 3427-4278"
                     /translation="HPIIYRGAVYADNMEFEQCIKLWLHALHLRQKGNRNTHKDLLRFAQVFSQMIHLNEAVKAPDIECVLRCSVLEIEQSMNRVKNISDADVHSAMDNYECNLYTFLYLVCISTKTQCSEEDQCRINKQIYNLIHLDPRTREGFSLLHLAVNSNTPVDDFHTNDVCSFPNALVTKLLLDCGAEVNAVDNEGNSALHIIVQYNRPISDFLTLHSIIISLVEAGAHTDMTNKQNKTPLDKSTTGVSEILLKTQMKMSAKCLAARAVRANDINYQDQIPRTLEEFVGFH*"
     misc_feature    complement(3505..3525)
                     /label="TPR-rev"
     misc_feature    3596..3620
                     /label="oAGM 476"
     misc_feature    3841..3975
                     /label="ANK7"
     misc_feature    complement(3955..3975)
                     /label="ank7-rev"
     misc_feature    3985..4098
                     /label="ANK8"
     misc_feature    complement(4078..4098)
                     /label="ANK8-rev"
     misc_feature    4195..4219
                     /label="oAGM 477"
     misc_feature    4318..4437
                     /label="SV40_PA_terminator"
     misc_feature    complement(4590..4605)
                     /label="M13R"
     misc_feature    5755..586
                     /label="amp"
ORIGIN
        1 AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA
       61 CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA
      121 TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA
      181 AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT
      241 GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA
      301 GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG
      361 CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC
      421 TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA
      481 TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT
      541 GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT
      601 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT
      661 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC
      721 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT
      781 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG
      841 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT
      901 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA
      961 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA
     1021 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA
     1081 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC
     1141 GCGAATTTTA ACAAAATATT AACGCTTACA ATTTCCATTC GCCATTCAGG CTGCGCAACT
     1201 GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG CCAGTCGACC ATAGCCAATT
     1261 CAATATGGCG TATATGGACT CATGCCAATT CAATATGGTG GATCTGGACC TGTGCCAATT
     1321 CAATATGGCG TATATGGACT CGTGCCAATT CAATATGGTG GATCTGGACC CCAGCCAATT
     1381 CAATATGGCG GACTTGGCAC CATGCCAATT CAATATGGCG GACTTGGCAC TGTGCCAACT
     1441 GGGGAGGGGT CTACTTGGCA CGGTGCCAAG TTTGAGGAGG GGTCTTGGCC CTGTGCCAAG
     1501 TCCGCCATAT TGAATTGGCA TGGTGCCAAT AATGGCGGCC ATATTGGCTA TATGCCAGGA
     1561 TCAATATATA GGCAATATCC AATATGGCCC TATGCCAATA TGGCTATTGG CCAGGTTCAA
     1621 TACTATGTAT TGGCCCTATG CCATATAGTA TTCCATATAT GGGTTTTCCT ATTGACGTAG
     1681 ATAGCCCCTC CCAATGGGCG GTCCCATATA CCATATATGG GGCTTCCTAA TACCGCCCAT
     1741 AGCCACTCCC CCATTGACGT CAATGGTCTC TATATATGGT CTTTCCTATT GACGTCATAT
     1801 GGGCGGTCCT ATTGACGTAT ATGGCGCCTC CCCCATTGAC GTCAATTACG GTAAATGGCC
     1861 CGCCTGGCTC AATGCCCATT GACGTCAATA GGACCACCCA CCATTGACGT CAATGGGATG
     1921 GCTCATTGCC CATTCATATC CGTTCTCACG CCCCCTATTG ACGTCAATGA CGGTAAATGG
     1981 CCCACTTGGC AGTACATCAA TATCTATTAA TAGTAACTTG GCAAGTACAT TACTATTGGA
     2041 AGGACGCCAG GGTACATTGG CAGTACTCCC ATTGACGTCA ATGGCGGTAA ATGGCCCGCG
     2101 ATGGCTGCCA AGTACATCCC CATTGACGTC AATGGGGAGG GGCAATGACG CAAATGGGCG
     2161 TTCCATTGAC GTAAATGGGC GGTAGGCGTG CCTAATGGGA GGTCTATATA AGCAATGCTC
     2221 GTTTAGGGAA CCGCCATTCT GCCTGGGGAC GTCGGAGCAA GCTTGATTTA GGTGACACTA
     2281 TAGAATACAA GCTACTTGTT CTTTTTGCAG ATGGACTACA AAGACCATGA CGGTGATTAT
     2341 AAAGATCATG ACATCGATTA CAAGGATGAC GATGACAAGG GCGGAGGAGT CGACATGGAG
     2401 GGCCTGGCTG GCTATGTGTA CAAGGCGGCC AGCGAGGGCA AGGTGCTCAC TCTGGCTGCC
     2461 TTGCTCCTTA ACCGGTCAGA GAGCGATATC CGCTACCTGC TGGGCTATGT CAGTCAGCAG
     2521 GGAGGACAGC GCTCCACACC CCTCATCATC GCAGCCCGCA ATGGGCACGC CAAGGTGGTG
     2581 CGCTTGCTGT TAGAACACTA CCGTGTGCAG ACCCAGCAGA CCGGCACCGT CCGCTTCGAC
     2641 GGGTATGTCa ttgacGGTGC CACTGCTCTT TGGTGTGCtG CgGGAGCcGG ACATTTTGAA
     2701 GTTGTTAAAC TTCTAGTCAG CCATGGAGCC AACGTGAACC AcACCACAGT cACTAAcTCA
     2761 ACtCCatTGC gGGCAGCATG CtttGATGGC AGACTGGACA TTGTGAAATA tTTGGTTGAA
     2821 AATAATGCCA ATATCAGCAT TGCCAACAAG TATGACaacA CCTGCCTAAT GATcGCaGCA
     2881 TATaaaGGGC ACACTGATGT GGTCAGATAC CTcTTAGAAC AACGTGCTGA TCCCAATGCt
     2941 AAAGCACAcT GTGGAGCCAC AGCtTTGCAC TTTGCAGCcG AAGCTGGtCA CATtGAcATT
     3001 GTGAAAGAaC TGATAAAATG GaGaGCTGCa ATAGTgGTGA ATGGCCATGG GATGACaCCA
     3061 TTGAAgGTgG CcGCtGAAAG CTGTAAAGCT GATGTCGTtG AACTGTTgCT CTCTCATGCT
     3121 GATTGtGACC GcAGAAGTCG GATTGAAGCc TTGGAgCTCT TGGGTGCCTC CTTTGCAAAT
     3181 GAtCGTGAGA ACTATGACAT CATgAAGACA TACCACTATt TATATTTAGC tATGTTgGAG
     3241 AGaTTtCAgG ATGGTGAcAA CATTCTtGAA AAAGAGGTTC TcCCACCcAT CCATGCTTAT
     3301 GGGAAcAGAA CTGAgTGTAG gAAcCCaCAG GAAtTGGAGg CtATTCGGCA AGACAGAGAT
     3361 GCTCTTCAcA TGGAgGGCCT TATAGTTCGG GAACGGATTT TAGGTGCTGA CAAcATTGAT
     3421 GTTTCcCAcC CCATCATTTA CAGAGGgGCT GTcTATGCtG ATAAcATGGA gTTcGAGCAG
     3481 TGcATCAAaT TGTGGCTcCA cGCaCTaCAC CTgAGgCAgA AAGGTAACAG GAAtACCCAC
     3541 AAaGATCTgC TTCGgTTTGC TCAAGTcTTC TCtCAgATGA TACAccTcAA TGAAgCTGTG
     3601 AAGGCCCCAG ACATAGAgTG cGTTTTGAGA TGCAGTGTcT TGGAAATAGA gCAgAGcATG
     3661 AACAGAGTtA AAAATATcTC cGATGCcGAc GTCCACAgTG CcATGGAtAA cTATGAgTGT
     3721 AAcCTCTATA CCTTTCTGTA ccTcGTGTGC ATCTCcACCA AgACACAGTG tAGCGAAGAA
     3781 GATCAGTGCA gAATTAACAA GCAGATCTAC AACCTGATTC ACCTgGAcCC CAGAACaCGg
     3841 GAAGGTTTtt CCTTGCTaCA cCTGGCTGTC AAcTCgAAcA CaCCAGTTGA TGATTTCCAt
     3901 ACtAAcGAtG TCTGCAGCTT TCCcAAcGCt CTgGTCACAA AGCTCCTGCT GGACTGTGGc
     3961 GCTGAGGTaA ATGCaGTGGA CAATGAGGGg AACAGTGCCC TcCAcATTAT CGTcCAGTAC
     4021 AACAGGCCCA TCAGTGATTT TcTGACtTTG CACTCCATCA TCATcAGCCT tGTgGAgGCt
     4081 GGAGCTCACA CcGACATGAC aAAcAAgCAG AATAAaACTC CGCTAGACAA AAGTACAACT
     4141 GGGGTgTCTG AAATACTaCT TAAAACTCAg ATGAAGATGA GcGCCAAGTG CCTGGCTGCC
     4201 CGAGCAGTTC GGGCTAATGA CATTAACTAC CAAGACCAGA TCCCCcGgAC TCTTGAAGAG
     4261 TTTGTTGGAT TTCATTAAtC TAGAACTATA GTGAGTCGTA TTACGTAGAT CCAGACATGA
     4321 TAAGATACAT TGATGAGTTT GGACAAACCA CAACTAGAAT GCAGTGAAAA AAATGCTTTA
     4381 TTTGTGAAAT TTGTGATGCT ATTGCTTTAT TTGTAACCAT TATAAGCTGC AATAAACAAG
     4441 TTAACAACAA CAATTGCATT CATTTTATGT TTCAGGTTCA GGGGGAGGTG TGGGAGGTTT
     4501 TTTAATTCGC GGCCGCGGCG CCAATGCATT GGGCCCGGTA CCCAGCTTTT GTTCCCTTTA
     4561 GTGAGGGTTA ATTGCGCGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG
     4621 TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG
     4681 TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC
     4741 GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT
     4801 GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT
     4861 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA
     4921 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC
     4981 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG
     5041 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG
     5101 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT
     5161 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT
     5221 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG
     5281 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT
     5341 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT
     5401 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT
     5461 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC
     5521 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC
     5581 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG
     5641 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA
     5701 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA
     5761 ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC
     5821 CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC
     5881 TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC
     5941 AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT
     6001 TAATTGTTGC CGGGAAGCTA GAGTAAGT
//