LOCUS       pCS2-GFP-degron-IRES-mC 6125 bp ds-DNA     circular     05-NOV-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    35..52
                     /label="SP6 F primer"
     misc_feature    78..83
                     /label="New Feature"
     misc_feature    91..108
                     /label="F"
     misc_feature    91..807
                     /label="GFP"
     CDS             91..807
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDDGNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYFGFVTAAAITHGMDELYK"
     misc_feature    808..822
                     /label="Gly Ser linker"
     CDS             808..831
                     /label="Translation 808-831"
                     /translation="GGGGSGDG"
     misc_feature    823..841
                     /label="spacer"
     misc_feature    843..845
                     /label="stop"
     misc_feature    846..851
                     /label="New Feature"
     misc_feature    846..851
                     /label="New Feature"
     misc_feature    852..1425
                     /label="IRES"
     misc_feature    1426..1433
                     /label="IRES"
     misc_feature    1434..2144
                     /label="mCherry"
     misc_feature    complement(2125..2144)
                     /label="R"
     misc_feature    2145..2150
                     /label="New Feature"
ORIGIN
        1 CGCCATTCTG CCTGGGGACG TCGGAGCAAG CTTGATTTAG GTGACACTAT AGAATACAAG
       61 CTACTTGTTC TTTTTGCGGA TCCCGCCACC ATGGTGAGCA AGGGCGCCGA GCTGTTCACC
      121 GGCATCGTGC CCATCCTGAT CGAGCTGAAT GGCGATGTGA ATGGCCACAA GTTCAGCGTG
      181 AGCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGTT CATCTGCACC
      241 ACCGGCAAGC TGCCTGTGCC CTGGCCCACC CTGGTGACCA CCCTGAGCTA CGGCGTGCAG
      301 TGCTTCTCAC GCTACCCCGA TCACATGAAG CAGCACGACT TCTTCAAGAG CGCCATGCCT
      361 GAGGGCTACA TCCAGGAGCG CACCATCTTC TTCGAGGATG ACGGCAACTA CAAGTCGCGC
      421 GCCGAGGTGA AGTTCGAGGG CGATACCCTG GTGAATCGCA TCGAGCTGAC CGGCACCGAT
      481 TTCAAGGAGG ATGGCAACAT CCTGGGCAAT AAGATGGAGT ACAACTACAA CGCCCACAAT
      541 GTGTACATCA TGACCGACAA GGCCAAGAAT GGCATCAAGG TGAACTTCAA GATCCGCCAC
      601 AACATCGAGG ATGGCAGCGT GCAGCTGGCC GACCACTACC AGCAGAATAC CCCCATCGGC
      661 GATGGCCCTG TGCTGCTGCC CGATAACCAC TACCTGTCCA CCCAGAGCGC CCTGTCCAAG
      721 GACCCCAACG AGAAGCGCGA TCACATGATC TACTTCGGCT TCGTGACCGC CGCCGCCATC
      781 ACCCACGGCA TGGATGAGCT GTACAAGgga ggcggaggca gcgGAGACGg acgaaCGTCT
      841 CtTGATCTAG AGCCCCTCTC CCTCCCCCCC CCCTAACGTT ACTGGCCGAA GCCGCTTGGA
      901 ATAAGGCCGG TGTGCGTTTG TCTATATGTT ATTTTCCACC ATATTGCCGT CTTTTGGCAA
      961 TGTGAGGGCC CGGAAACCTG GCCCTGTCTT CTTGACGAGC ATTCCTAGGG GTCTTTCCCC
     1021 TCTCGCCAAA GGAATGCAAG GTCTGTTGAA TGTCGTGAAG GAAGCAGTTC CTCTGGAAGC
     1081 TTCTTGAAGA CAAACAACGT CTGTAGCGAC CCTTTGCAGG CAGCGGAACC CCCCACCTGG
     1141 CGACAGGTGC CTCTGCGGCC AAAAGCCACG TGTATAAGAT ACACCTGCAA AGGCGGCACA
     1201 ACCCCAGTGC CACGTTGTGA GTTGGATAGT TGTGGAAAGA GTCAAATGGC TCTCCTCAAG
     1261 CGTATTCAAC AAGGGGCTGA AGGATGCCCA GAAGGTACCC CATTGTATGG GATCTGATCT
     1321 GGGGCCTCGG TGCACATGCT TTACATGTGT TTAGTCGAGG TTAAAAAAAC GTCTAGGCCC
     1381 CCCGAACCAC GGGGACGTGG TTTTCCTTTG AAAAACACGA TAATACCTCC GGAATGGTGA
     1441 GCAAGGGCGA GGAGGATAAC ATGGCCATCA TCAAGGAGTT CATGCGCTTC AAGGTGCACA
     1501 TGGAGGGCTC CGTGAACGGC CACGAGTTCG AGATCGAGGG CGAGGGCGAG GGCCGCCCCT
     1561 ACGAGGGCAC CCAGACCGCC AAGCTGAAGG TGACCAAGGG TGGCCCCCTG CCCTTCGCCT
     1621 GGGACATCCT GTCCCCTCAG TTCATGTACG GCTCCAAGGC CTACGTGAAG CACCCCGCCG
     1681 ACATCCCCGA CTACTTGAAG CTGTCCTTCC CCGAGGGCTT CAAGTGGGAG CGCGTGATGA
     1741 ACTTCGAGGA CGGCGGCGTG GTGACCGTGA CCCAGGACTC CTCCCTGCAG GACGGCGAGT
     1801 TCATCTACAA GGTGAAGCTG CGCGGCACCA ACTTCCCCTC CGACGGCCCC GTAATGCAGA
     1861 AGAAGACCAT GGGCTGGGAG GCCTCCTCCG AGCGGATGTA CCCCGAGGAC GGCGCCCTGA
     1921 AGGGCGAGAT CAAGCAGAGG CTGAAGCTGA AGGACGGCGG CCACTACGAC GCTGAGGTCA
     1981 AGACCACCTA CAAGGCCAAG AAGCCCGTGC AGCTGCCCGG CGCCTACaAC GTCAACATCA
     2041 AGTTGGACAT CACCTCCCAC AACGAGGACT ACACCATCGT GGAACAGTAC GAACGCGCCG
     2101 AGGGCCGCCA CTCCACCGGC GGCATGGACG AGCTGTACAA GTGATCTAGA ACTATAGTGA
     2161 GTCGTATTAC GTAGATCCAG ACATGATAAG ATACATTGAT GAGTTTGGAC AAACCACAAC
     2221 TAGAATGCAG TGAAAAAAAT GCTTTATTTG TGAAATTTGT GATGCTATTG CTTTATTTGT
     2281 AACCATTATA AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA
     2341 GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA ATTCGCGGCC GCGGCGCCAA TGCATTGGGC
     2401 CCGGTACCCA GCTTTTGTTC CCTTTAGTGA GGGTTAATTG CGCGCTTGGC GTAATCATGG
     2461 TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC
     2521 GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG
     2581 TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC
     2641 GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT
     2701 GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA
     2761 ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG
     2821 CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC
     2881 CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA
     2941 TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG
     3001 CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC
     3061 TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC
     3121 GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC
     3181 CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG
     3241 AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA
     3301 AGGACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT
     3361 AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG
     3421 CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT
     3481 GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG
     3541 ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT
     3601 GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC
     3661 TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG
     3721 GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT
     3781 CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA
     3841 ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG
     3901 CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG
     3961 TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC
     4021 CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG
     4081 TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG
     4141 CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG
     4201 TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT
     4261 AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG
     4321 ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA
     4381 GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA
     4441 AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT
     4501 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG
     4561 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TAAATTGTAA
     4621 GCGTTAATAT TTTGTTAAAA TTCGCGTTAA ATTTTTGTTA AATCAGCTCA TTTTTTAACC
     4681 AATAGGCCGA AATCGGCAAA ATCCCTTATA AATCAAAAGA ATAGACCGAG ATAGGGTTGA
     4741 GTGTTGTTCC AGTTTGGAAC AAGAGTCCAC TATTAAAGAA CGTGGACTCC AACGTCAAAG
     4801 GGCGAAAAAC CGTCTATCAG GGCGATGGCC CACTACGTGA ACCATCACCC TAATCAAGTT
     4861 TTTTGGGGTC GAGGTGCCGT AAAGCACTAA ATCGGAACCC TAAAGGGAGC CCCCGATTTA
     4921 GAGCTTGACG GGGAAAGCCG GCGAACGTGG CGAGAAAGGA AGGGAAGAAA GCGAAAGGAG
     4981 CGGGCGCTAG GGCGCTGGCA AGTGTAGCGG TCACGCTGCG CGTAACCACC ACACCCGCCG
     5041 CGCTTAATGC GCCGCTACAG GGCGCGTCCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG
     5101 AAGGGCGATC GGTGCGGGCC TCTTCGCTAT TACGCCAGTC GACCATAGCC AATTCAATAT
     5161 GGCGTATATG GACTCATGCC AATTCAATAT GGTGGATCTG GACCTGTGCC AATTCAATAT
     5221 GGCGTATATG GACTCGTGCC AATTCAATAT GGTGGATCTG GACCCCAGCC AATTCAATAT
     5281 GGCGGACTTG GCACCATGCC AATTCAATAT GGCGGACTTG GCACTGTGCC AACTGGGGAG
     5341 GGGTCTACTT GGCACGGTGC CAAGTTTGAG GAGGGGTCTT GGCCCTGTGC CAAGTCCGCC
     5401 ATATTGAATT GGCATGGTGC CAATAATGGC GGCCATATTG GCTATATGCC AGGATCAATA
     5461 TATAGGCAAT ATCCAATATG GCCCTATGCC AATATGGCTA TTGGCCAGGT TCAATACTAT
     5521 GTATTGGCCC TATGCCATAT AGTATTCCAT ATATGGGTTT TCCTATTGAC GTAGATAGCC
     5581 CCTCCCAATG GGCGGTCCCA TATACCATAT ATGGGGCTTC CTAATACCGC CCATAGCCAC
     5641 TCCCCCATTG ACGTCAATGG TCTCTATATA TGGTCTTTCC TATTGACGTC ATATGGGCGG
     5701 TCCTATTGAC GTATATGGCG CCTCCCCCAT TGACGTCAAT TACGGTAAAT GGCCCGCCTG
     5761 GCTCAATGCC CATTGACGTC AATAGGACCA CCCACCATTG ACGTCAATGG GATGGCTCAT
     5821 TGCCCATTCA TATCCGTTCT CACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCACT
     5881 TGGCAGTACA TCAATATCTA TTAATAGTAA CTTGGCAAGT ACATTACTAT TGGAAGGACG
     5941 CCAGGGTACA TTGGCAGTAC TCCCATTGAC GTCAATGGCG GTAAATGGCC CGCGATGGCT
     6001 GCCAAGTACA TCCCCATTGA CGTCAATGGG GAGGGGCAAT GACGCAAATG GGCGTTCCAT
     6061 TGACGTAAAT GGGCGGTAGG CGTGCCTAAT GGGAGGTCTA TATAAGCAAT GCTCGTTTAG
     6121 GGAAC
//