LOCUS       pCS2_GFP_CDK5R1_degron_ 6180 bp ds-DNA     circular     28-OCT-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    78..83
                     /label="New Feature"
     misc_feature    91..807
                     /label="GFP"
     misc_feature    808..818
                     /label="Gly Ser linker"
     CDS             808..822
                     /label="Translation 808-822"
                     /translation="GGGGS"
     misc_feature    819..822
                     /label="extra"
     misc_feature    823..897
                     /label="CDK5R1 degron"
     CDS             823..897
                     /label="Translation 823-897"
                     /translation="TQVFSDLKNESGQEDKKRLLLGLDR"
     misc_feature    898..900
                     /label="stop"
     misc_feature    901..906
                     /label="New Feature"
     misc_feature    901..906
                     /label="New Feature"
     misc_feature    907..1480
                     /label="IRES"
     misc_feature    1489..2199
                     /label="mCherry"
     misc_feature    2200..2205
                     /label="New Feature"
ORIGIN
        1 CGCCATTCTG CCTGGGGACG TCGGAGCAAG CTTGATTTAG GTGACACTAT AGAATACAAG
       61 CTACTTGTTC TTTTTGCGGA TCCCGCCACC ATGGTGAGCA AGGGCGCCGA GCTGTTCACC
      121 GGCATCGTGC CCATCCTGAT CGAGCTGAAT GGCGATGTGA ATGGCCACAA GTTCAGCGTG
      181 AGCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGTT CATCTGCACC
      241 ACCGGCAAGC TGCCTGTGCC CTGGCCCACC CTGGTGACCA CCCTGAGCTA CGGCGTGCAG
      301 TGCTTCTCAC GCTACCCCGA TCACATGAAG CAGCACGACT TCTTCAAGAG CGCCATGCCT
      361 GAGGGCTACA TCCAGGAGCG CACCATCTTC TTCGAGGATG ACGGCAACTA CAAGTCGCGC
      421 GCCGAGGTGA AGTTCGAGGG CGATACCCTG GTGAATCGCA TCGAGCTGAC CGGCACCGAT
      481 TTCAAGGAGG ATGGCAACAT CCTGGGCAAT AAGATGGAGT ACAACTACAA CGCCCACAAT
      541 GTGTACATCA TGACCGACAA GGCCAAGAAT GGCATCAAGG TGAACTTCAA GATCCGCCAC
      601 AACATCGAGG ATGGCAGCGT GCAGCTGGCC GACCACTACC AGCAGAATAC CCCCATCGGC
      661 GATGGCCCTG TGCTGCTGCC CGATAACCAC TACCTGTCCA CCCAGAGCGC CCTGTCCAAG
      721 GACCCCAACG AGAAGCGCGA TCACATGATC TACTTCGGCT TCGTGACCGC CGCCGCCATC
      781 ACCCACGGCA TGGATGAGCT GTACAAGgga ggcggaggca gcACACAGGT CTTCTCCGAC
      841 CTGAAGAACG AGAGCGGCCA GGAGGACAAG AAGCGGCTCC TCCTAGGCCT GGATCGGTGA
      901 TCTAGAGCCC CTCTCCCTCC CCCCCCCCTA ACGTTACTGG CCGAAGCCGC TTGGAATAAG
      961 GCCGGTGTGC GTTTGTCTAT ATGTTATTTT CCACCATATT GCCGTCTTTT GGCAATGTGA
     1021 GGGCCCGGAA ACCTGGCCCT GTCTTCTTGA CGAGCATTCC TAGGGGTCTT TCCCCTCTCG
     1081 CCAAAGGAAT GCAAGGTCTG TTGAATGTCG TGAAGGAAGC AGTTCCTCTG GAAGCTTCTT
     1141 GAAGACAAAC AACGTCTGTA GCGACCCTTT GCAGGCAGCG GAACCCCCCA CCTGGCGACA
     1201 GGTGCCTCTG CGGCCAAAAG CCACGTGTAT AAGATACACC TGCAAAGGCG GCACAACCCC
     1261 AGTGCCACGT TGTGAGTTGG ATAGTTGTGG AAAGAGTCAA ATGGCTCTCC TCAAGCGTAT
     1321 TCAACAAGGG GCTGAAGGAT GCCCAGAAGG TACCCCATTG TATGGGATCT GATCTGGGGC
     1381 CTCGGTGCAC ATGCTTTACA TGTGTTTAGT CGAGGTTAAA AAAACGTCTA GGCCCCCCGA
     1441 ACCACGGGGA CGTGGTTTTC CTTTGAAAAA CACGATAATA CCTCCGGAAT GGTGAGCAAG
     1501 GGCGAGGAGG ATAACATGGC CATCATCAAG GAGTTCATGC GCTTCAAGGT GCACATGGAG
     1561 GGCTCCGTGA ACGGCCACGA GTTCGAGATC GAGGGCGAGG GCGAGGGCCG CCCCTACGAG
     1621 GGCACCCAGA CCGCCAAGCT GAAGGTGACC AAGGGTGGCC CCCTGCCCTT CGCCTGGGAC
     1681 ATCCTGTCCC CTCAGTTCAT GTACGGCTCC AAGGCCTACG TGAAGCACCC CGCCGACATC
     1741 CCCGACTACT TGAAGCTGTC CTTCCCCGAG GGCTTCAAGT GGGAGCGCGT GATGAACTTC
     1801 GAGGACGGCG GCGTGGTGAC CGTGACCCAG GACTCCTCCC TGCAGGACGG CGAGTTCATC
     1861 TACAAGGTGA AGCTGCGCGG CACCAACTTC CCCTCCGACG GCCCCGTAAT GCAGAAGAAG
     1921 ACCATGGGCT GGGAGGCCTC CTCCGAGCGG ATGTACCCCG AGGACGGCGC CCTGAAGGGC
     1981 GAGATCAAGC AGAGGCTGAA GCTGAAGGAC GGCGGCCACT ACGACGCTGA GGTCAAGACC
     2041 ACCTACAAGG CCAAGAAGCC CGTGCAGCTG CCCGGCGCCT ACaACGTCAA CATCAAGTTG
     2101 GACATCACCT CCCACAACGA GGACTACACC ATCGTGGAAC AGTACGAACG CGCCGAGGGC
     2161 CGCCACTCCA CCGGCGGCAT GGACGAGCTG TACAAGTGAT CTAGAACTAT AGTGAGTCGT
     2221 ATTACGTAGA TCCAGACATG ATAAGATACA TTGATGAGTT TGGACAAACC ACAACTAGAA
     2281 TGCAGTGAAA AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA
     2341 TTATAAGCTG CAATAAACAA GTTAACAACA ACAATTGCAT TCATTTTATG TTTCAGGTTC
     2401 AGGGGGAGGT GTGGGAGGTT TTTTAATTCG CGGCCGCGGC GCCAATGCAT TGGGCCCGGT
     2461 ACCCAGCTTT TGTTCCCTTT AGTGAGGGTT AATTGCGCGC TTGGCGTAAT CATGGTCATA
     2521 GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG
     2581 CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG
     2641 CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA
     2701 ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC
     2761 GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG
     2821 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA
     2881 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA
     2941 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG
     3001 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT
     3061 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG
     3121 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC
     3181 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT
     3241 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA
     3301 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC
     3361 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC
     3421 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT
     3481 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC
     3541 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT
     3601 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA
     3661 AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT
     3721 ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG
     3781 CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA
     3841 TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT
     3901 ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT
     3961 TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT
     4021 TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT
     4081 GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC
     4141 CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC
     4201 CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT
     4261 GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG
     4321 AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT
     4381 ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC
     4441 TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA
     4501 GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG
     4561 AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA
     4621 TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTAAAT TGTAAGCGTT
     4681 AATATTTTGT TAAAATTCGC GTTAAATTTT TGTTAAATCA GCTCATTTTT TAACCAATAG
     4741 GCCGAAATCG GCAAAATCCC TTATAAATCA AAAGAATAGA CCGAGATAGG GTTGAGTGTT
     4801 GTTCCAGTTT GGAACAAGAG TCCACTATTA AAGAACGTGG ACTCCAACGT CAAAGGGCGA
     4861 AAAACCGTCT ATCAGGGCGA TGGCCCACTA CGTGAACCAT CACCCTAATC AAGTTTTTTG
     4921 GGGTCGAGGT GCCGTAAAGC ACTAAATCGG AACCCTAAAG GGAGCCCCCG ATTTAGAGCT
     4981 TGACGGGGAA AGCCGGCGAA CGTGGCGAGA AAGGAAGGGA AGAAAGCGAA AGGAGCGGGC
     5041 GCTAGGGCGC TGGCAAGTGT AGCGGTCACG CTGCGCGTAA CCACCACACC CGCCGCGCTT
     5101 AATGCGCCGC TACAGGGCGC GTCCCATTCG CCATTCAGGC TGCGCAACTG TTGGGAAGGG
     5161 CGATCGGTGC GGGCCTCTTC GCTATTACGC CAGTCGACCA TAGCCAATTC AATATGGCGT
     5221 ATATGGACTC ATGCCAATTC AATATGGTGG ATCTGGACCT GTGCCAATTC AATATGGCGT
     5281 ATATGGACTC GTGCCAATTC AATATGGTGG ATCTGGACCC CAGCCAATTC AATATGGCGG
     5341 ACTTGGCACC ATGCCAATTC AATATGGCGG ACTTGGCACT GTGCCAACTG GGGAGGGGTC
     5401 TACTTGGCAC GGTGCCAAGT TTGAGGAGGG GTCTTGGCCC TGTGCCAAGT CCGCCATATT
     5461 GAATTGGCAT GGTGCCAATA ATGGCGGCCA TATTGGCTAT ATGCCAGGAT CAATATATAG
     5521 GCAATATCCA ATATGGCCCT ATGCCAATAT GGCTATTGGC CAGGTTCAAT ACTATGTATT
     5581 GGCCCTATGC CATATAGTAT TCCATATATG GGTTTTCCTA TTGACGTAGA TAGCCCCTCC
     5641 CAATGGGCGG TCCCATATAC CATATATGGG GCTTCCTAAT ACCGCCCATA GCCACTCCCC
     5701 CATTGACGTC AATGGTCTCT ATATATGGTC TTTCCTATTG ACGTCATATG GGCGGTCCTA
     5761 TTGACGTATA TGGCGCCTCC CCCATTGACG TCAATTACGG TAAATGGCCC GCCTGGCTCA
     5821 ATGCCCATTG ACGTCAATAG GACCACCCAC CATTGACGTC AATGGGATGG CTCATTGCCC
     5881 ATTCATATCC GTTCTCACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCACTTGGCA
     5941 GTACATCAAT ATCTATTAAT AGTAACTTGG CAAGTACATT ACTATTGGAA GGACGCCAGG
     6001 GTACATTGGC AGTACTCCCA TTGACGTCAA TGGCGGTAAA TGGCCCGCGA TGGCTGCCAA
     6061 GTACATCCCC ATTGACGTCA ATGGGGAGGG GCAATGACGC AAATGGGCGT TCCATTGACG
     6121 TAAATGGGCG GTAGGCGTGC CTAATGGGAG GTCTATATAA GCAATGCTCG TTTAGGGAAC
//