LOCUS       Exported                9360 bp ds-DNA     circular SYN 18-NOV-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 9360)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Nov 19, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9360
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    679..796
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    854..881
                     /label=bGH
     primer_bind     complement(871..893)
                     /label=Primer 4
     polyA_signal    882..1078
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     1057..1083
                     /label=Primer 5
     misc_feature    1079
                     /label=Barcode_Cloning_Site
     primer_bind     complement(1166..1185)
                     /label=Primer 6
     misc_feature    1166
                     /label=Linker
     misc_feature    1167..1186
                     /label=BFP_Perturb_Primer
     primer_bind     complement(1168..1199)
                     /label=Oligo_19
     misc_feature    1187
                     /label=GFP homology
     primer_bind     1196..1214
                     /label=Oligo_41
     primer_bind     1200..1214
                     /label=Oligo_42
     misc_feature    1201..1789
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             1307..1318
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1716..1736
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     primer_bind     complement(1778..1824)
                     /label=Oligo_40
     primer_bind     complement(1778..1802)
                     /label=Primer 2
     primer_bind     complement(1802..1822)
                     /label=x6
     primer_bind     1817..1854
                     /label=oG-reverse-cloneseq-overhang
     CDS             complement(1831..3405)
                     /codon_start=1
                     /label=HumOpt oG
                     /translation="MVPQALLFVPLLVFPLCFGKFPIYTIPDKLGPWSPIDIHHLSCPN
                     NLVVEDEGCTNLSGFSYMELKVGYISAIKMNGFTCTGVVTEAETYTNFVGYVTTTFKRK
                     HFRPTPDACRAAYNWKMAGDPRYEESLHNPYPDYHWLRTVKTTKESLVIISPSVADLDP
                     YDRSLHSPVFPGGNCSGVAVSSTYCSTNHDYTIWMPENPRLGMSCDIFTNSRGKRASKG
                     SETCGFVDERGLYKSLKGACKLKLCGVLGLRLMDGTWVAMQTSNETKWCPPGQLVNLHD
                     FRSDEIEHLVVEELVKKREECLDALESIMTTKSVSFRRLSHLRKLVPGFGKAYTIFNKT
                     LMEADAHYKSVRTWNEIIPSKGCLRVGGRCHPHVNGVFFNGIILGPDGNVLIPEMQSSL
                     LQQHMELLVSSVIPLMHPLADPSTVFKNGDEAEDFVEVHLPDVHERISGVDLGLPNWGK
                     YVLLSAGALTALMLIIFLMTCWRRVNRSEPTQHNLRGTGREVSVTPQSGKIISSWESHK
                     SGGETRL"
     misc_feature    2708..2714
                     /label=GGGAAAA?
     misc_feature    2747
                     /label=Feature 31
     primer_bind     complement(3386..3417)
                     /label=oG-F-addSbfI-MCS
     primer_bind     3410..3447
                     /label=TVA-R-addE2A-MCS
     misc_feature    3415..3474
                     /label=HumOpt E2A
     primer_bind     3427..3501
                     /label=Primer attach humoptE2A-oG-5'
     primer_bind     3475..3493
                     /label=TVA-R-addP2A
     CDS             complement(3484..3954)
                     /codon_start=1
                     /label=TVA_human_optimized
                     /translation="MARLLPALLLLLLPGNVTGNGSGNGSLSRCPPGQFRCSEPPGAHG
                     ECYPQDWLCDGHPDCDDGRDEWGCGTSAIPAVPTDNGTEAPTAPAPGRALPARNHGRMW
                     MLITAVLLCCLVAVGGIAAWGKSKAKSRSDIFSLESASKELLVPDKSQADLFS"
     primer_bind     3484..3504
                     /label=Primer 2
     primer_bind     complement(3633..3653)
                     /label=TVA-F
     primer_bind     complement(3935..4037)
                     /label=Attach T2A-GFP BsrGI site
     primer_bind     complement(3935..4023)
                     /label=Primer 3
     primer_bind     complement(3937..4037)
                     /label=Primer 1
     misc_feature    3961..4014
                     /label=T2A
     primer_bind     3961..3980
                     /label=dualpromoter-R5v2
                     /note="CCTGCAGG is SbfI"
     CDS             complement(4024..4740)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    4054
                     /label=A?
     primer_bind     complement(4318..4329)
                     /label=Oligo_19
     primer_bind     complement(4729..4746)
                     /label=dualpromoter-F5v2
                     /note="Destroy AgeI due to high GC, replace with XbaI 
                     (aTCTAGA), overlap with short CAG promoter"
     primer_bind     4748..4779
                     /label=hSyn-R-attachCloneSeqAgeI
     primer_bind     4754..4778
                     /label=dualpromoter-R2
                     /note="30bp overlap with TVA"
     promoter        complement(4761..5208)
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     primer_bind     complement(5188..5216)
                     /label=hSyn-F-addBstEIInovel
     primer_bind     complement(5195..5216)
                     /label=dualpromoter-F2v2
     primer_bind     5209..5230
                     /label=CloneSeqU3-R-addBstEII
     primer_bind     5209..5230
                     /label=x5
     misc_feature    5281..5600
                     /label=U3
     primer_bind     complement(5580..5599)
                     /label=CloneSeq-U3-F
     LTR             5601..5781
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(5843..6431)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5923..5942)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6602..7462)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7225..7244
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7463..7567)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7642..7661)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7744..7761)
                     /label=dualpromoter F2
     enhancer        7833..8212
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     7908..7925
                     /label=[P] hSyn 15bp +  (Bst EII + 40bp + [CAG] + Bsu36I)
     promoter        8214..8412
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             8430..8610
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8657..8782
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    join(9279..9360,1..152)
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
       61 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
      121 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
      181 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
      241 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
      301 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
      361 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
      421 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
      481 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
      541 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
      601 gacagatcca ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat
      661 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
      721 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
      781 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt ggttagtacc
      841 gggcccgctc tagccataga gcccaccgca tccccagcat gcctgctatt gtcttcccaa
      901 tcctccccct tgctgtcctg ccccacccca ccccccagaa tagaatgaca cctactcaga
      961 caatgcgatg caatttcctc attttattag gaaaggacag tgggagtggc accttccagg
     1021 gtcaaggaag gcacggggga ggggcaaaca acagatggct ggcaactaga aggcacagct
     1081 cgagtagaca tgtcgagcta gccaatgatc atacggcgcg ccgaaggtac ccttggatcc
     1141 ctacctaggt gggaattcga taattcgctt gtgccccagt ttgctaggga ggtccaggac
     1201 gcggggaggc ggcccaaagg gagatccgac tcgtctgagg gcgaaggcga agacgcggaa
     1261 gaggccgcag agccggcagc aggccgcggg aaggaaggtc cgctggattg agggccgaag
     1321 ggacgtagca gaaggacgtc ccgcgcagaa tccaggtggc aacacaggcg agcagccaag
     1381 gaaaggacga tgatttcccc gacaacacca cggaattgtc agtgcccaac agccgagccc
     1441 ctgtccagca gcgggcaagg caggcggcga tgagttccgc cgtggcaata gggaggggga
     1501 aagcgaaagt cccggaaagg agctgacagg tggtggcaat gccccaacca gtgggggttg
     1561 cgtcagcaaa cacagtgcac accacgccac gttgcctgac aacgggccac aactcctcat
     1621 aaagagacag caaccaggat ttatacaagg aggagaaaat gaaagccata cgggaagcaa
     1681 tagcatgata caaaggcatt aaagcagcgt atccacatag cgtaaaagga gcaacatagt
     1741 taagaatacc agtcaatctt tcacaaattt tgtaatccag aggttgattg gatcgtacgt
     1801 cggcataagc tttgtagcgg ccgctacaaa ttacagtcgg gtctcaccac cagacttatg
     1861 gctctcccag gaagatatga ttttcccgga ttgtggagtt acagacactt cccgacctgt
     1921 ccctcgcagg ttatgttggg ttggttcgga tctgttcact ctccgccagc acgtcatgag
     1981 gaatattatc agcatgaggg cggtcaacgc tccggcactg agaagaacgt acttgcccca
     2041 gtttggaagc ccgaggtcta ccccactgat tcgttcatgt acatctggca agtggacctc
     2101 tacaaagtct tcggcctcat caccattttt gaataccgta ctggggtctg cgagaggatg
     2161 cataagtggt atgacggaag aaacgagcaa ctccatgtgt tgttgcaaaa gggagctttg
     2221 catctctgga atcagcacgt tgccgtcggg gccaaggata ataccattga agaatacgcc
     2281 gtttacgtgg gggtggcatc taccaccaac ccgcaagcac cccttggatg gtatgatctc
     2341 gttccaagtc cgaacagact tgtaatgcgc gtcagcctcc atgagagttt tgttgaaaat
     2401 tgtatatgct ttcccaaagc ctggtacaag tttcctaaga tgggacaacc ggcggaaaga
     2461 aacagattta gtagtcataa tagattcgag agcgtccagg cactcttctc tcttcttcac
     2521 aagttcttca acgaccaaat gttctatttc atcactccgg aagtcgtgga gattcaccag
     2581 ctggcctggt ggacaccact ttgtctcgtt ggaagtttgc atcgccaccc aggttccatc
     2641 catcaaccga agaccaagga cgccgcagag tttgagtttg caggcacctt tcaatgattt
     2701 gtaaaggccc ctttcatcta caaatccaca cgtctcacta cccttggacg cccgcttgcc
     2761 gcgagagtta gtgaatatgt cacaactcat acccaacctg ggattctccg gcatccagat
     2821 cgtgtaatca tggttggtgc tgcaataggt ggaactcacc gctacgccgc tacaattgcc
     2881 gcccgggaac acaggactgt gcaggctccg gtcgtaaggg tccagatctg cgactgaggg
     2941 actaatgatt acgagggatt cttttgtcgt tttcacagtt cgcaaccaat ggtaatctgg
     3001 gtagggatta tgcagtgact cttcatacct cggatcgcca gccattttcc aattgtaagc
     3061 tgctcggcac gcgtcaggcg ttggtctaaa atgttttctt ttaaacgtgg tggttacata
     3121 cccgacaaag ttagtgtatg tctcagcttc agttacaaca cctgtacacg taaaaccatt
     3181 cattttgatg gctgaaatat accctacctt gagctccata taggagaacc cactcaagtt
     3241 ggtgcatccc tcatcttcta ctaccaggtt gttagggcaa gacaaatggt ggatgtcgat
     3301 aggagaccac ggaccgagct tatccggtat ggtgtaaata gggaatttac caaagcacag
     3361 tgggaacacc aacagaggaa cgaacaacag cgcttggggc accatggtgc ctgcaggacc
     3421 tgggtttgac tcgacgtctc cggccaattt gagcaaggca taattcgtgc actgtcctga
     3481 accactgaac aggtccgcct gactcttatc cgggacgaga agttccttag aagctgactc
     3541 caatgaaaaa atatcagagc ggctctttgc tttggacttg ccccatgcgg ctatcccccc
     3601 gacggctacg agacaacaga gcaaaaccgc tgttatcagc atccacatcc gtccgtgatt
     3661 ccgtgcggga agcgcccgac ctggagctgg agccgtgggt gcctcagttc cgttatccgt
     3721 aggcaccgca ggaattgcac ttgtaccaca gccccattca tctcttccat cgtcgcagtc
     3781 tggatgcccg tcgcaaagcc aatcctgtgg gtaacattcc ccatgtgcgc ccgggggctc
     3841 actacacctg aattgacctg gtggacaccg ggacaatgat ccattacctg agccgttccc
     3901 cgttacattg cctggcagca ggagcagaag aagggctgga agaagccttg ccatcgtgcg
     3961 aggcccagga ttctcctcca cgtcaccaca cgtaagcaag gaaccgcgcc cttccccgga
     4021 ccccttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga actccagcag
     4081 gaccatgtga tcgcgcttct cgttggggtc tttgctcagg gcggactggg tgctcaggta
     4141 gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct ggtagtggtc
     4201 ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc ttgaagttca ccttgatgcc
     4261 gttcttctgc ttgtcggcca tgatatagac gttgtggctg ttgtagttgt actccagctt
     4321 gtgccccagg atgttgccgt cctccttgaa gtcgatgccc ttcagctcga tgcggttcac
     4381 cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa
     4441 gaagatggtg cgctcctgga cgtagccttc gggcatggcg gacttgaaga agtcgtgctg
     4501 cttcatgtgg tcggggtagc ggctgaagca ctgcacgccg taggtcaggg tggtcacgag
     4561 ggtgggccag ggcacgggca gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc
     4621 gtaggtggca tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt ttacgtcgcc
     4681 gtccagctcg accaggatgg gcaccacccc ggtgaacagc tcctcgccct tgctcaccat
     4741 ggtggcgacc ggtttctcga ctgcgctctc aggcacgaca cgactcctcc gctgcccacc
     4801 gcagactgag gcagcgctga gtcgccggcg ccgcagcgca gatggtcgcg cccgtgcccc
     4861 cctatctcgc gcctcgcgtg gtgcggtccg gctgggccgg cggcggcgcg gacgcgacca
     4921 aggtggccgg gaaggggagt ttgcggggga ccggcgagtg acgtcagcgc gccttcagtg
     4981 ctgaggcggc ggtggcgcgc gccgccaggc gggggcgaag gcactgtccg cggtgctgaa
     5041 gctggcagtg cgcacgcgcc tcgccgcatc ctgtttcccc tccccctctc tgatagggga
     5101 tgcgcaattt ggggaatggg ggttgggtgc ttgtccagtg ggtcggggtc ggtcgtcagg
     5161 taggcacccc caccccgcct catcctggtc ctaaaaccca cttgcactgg tgacctttaa
     5221 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     5281 tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca
     5341 cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc agatatccac
     5401 tgacctttgg atggtgctac aagctagtac cagttgagca agagaaggta gaagaagcca
     5461 atgaaggaga gaacacccgc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
     5521 agagagaagt attagagtgg aggtttgaca gccgcctagc atttcatcac atggcccgag
     5581 agctgcatcc ggactgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     5641 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     5701 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     5761 cagtgtggaa aatctctagc agcatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     5821 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     5881 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     5941 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     6001 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     6061 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     6121 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     6181 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     6241 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     6301 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     6361 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     6421 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     6481 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     6541 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     6601 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     6661 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     6721 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     6781 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     6841 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     6901 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     6961 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     7021 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     7081 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     7141 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     7201 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     7261 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     7321 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     7381 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     7441 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     7501 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     7561 ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga
     7621 tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct
     7681 gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac
     7741 aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct
     7801 gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata
     7861 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
     7921 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     7981 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
     8041 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
     8101 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
     8161 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
     8221 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
     8281 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
     8341 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
     8401 ctatataagc agcgcgtttt gcctgtactg ggtctctctg gttagaccag atctgagcct
     8461 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     8521 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     8581 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
     8641 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
     8701 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
     8761 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
     8821 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     8881 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     8941 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     9001 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     9061 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     9121 ggccggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     9181 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     9241 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     9301 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
//
