LOCUS       Exported               10700 bp ds-DNA     circular SYN 07-JUN-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 10700)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Dec 9, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10700
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    579..704
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             751..931
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(949..1147)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        1149..1528
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     1700..1719
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        1794..1898
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1899..2759
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2117..2136)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2930..3518
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3419..3438
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             3580..3760
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3761..4080
                     /label=U3
     misc_feature    4171..4183
                     /label=CMV_enhancer
     enhancer        4184..4548
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4551..4826
                     /label=chicken beta-actin promoter
     primer_bind     complement(4662..4671)
                     /label=Oligo_131
     primer_bind     complement(4705..4785)
                     /label=Oligo_131
     intron          4827..5788
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     misc_feature    5789..5835
                     /label=chimeric intro
     primer_bind     5836..5857
                     /label=Primer 6
     primer_bind     5849..5873
                     /label=AgeI-F
     primer_bind     5867..5884
                     /label=dualpromoter-F5v2
                     /note="tctaga : XbaI site, destroy AgeI here."
     CDS             5873..6589
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     6284..6295
                     /label=Oligo_19
     misc_feature    6559
                     /label=A?
     misc_feature    6599..6652
                     /label=T2A
     primer_bind     6628..6672
                     /label=Primer 1
     primer_bind     complement(6633..6653)
                     /label=dualpromoter-R5v2
     primer_bind     complement(6633..6652)
                     /label=Primer 5
     misc_feature    6659..8878
                     /label=FOXP2
     primer_bind     complement(8854..8903)
                     /label=Primer 2
     primer_bind     8879..8923
                     /label=Oligo_40
     primer_bind     8899..8923
                     /label=Primer 2
     misc_feature    8912..9500
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8965..8985)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9383..9394)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9487..9505)
                     /label=Oligo_41
     primer_bind     complement(9487..9501)
                     /label=Oligo_42
     primer_bind     complement(9492..9501)
                     /label=Primer 1
     primer_bind     9502..9533
                     /label=Oligo_19
     misc_feature    9514
                     /label=GFP homology
     misc_feature    9515..9534
                     /label=BFP_Perturb_Primer
     misc_feature    9535
                     /label=Linker
     misc_feature    9622
                     /label=Barcode_Cloning_Site
     polyA_signal    9623..9819
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     9808..9830
                     /label=Primer 4
     misc_feature    9820..9847
                     /label=bGH
     misc_feature    9905..10022
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    join(10549..10700,1..82)
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 tctggcctgt accgtcagcg tcattgacgc tgcgcccata gtgcttcctg ctgctcccaa
       61 gaacccaagg aacaaagctc ctattcccac tgctcttttt tctctctgca ccactcttct
      121 ctttgccttg gtgggtgcta ctcctaatgg ttcaattttt actactttat atttatataa
      181 ttcacttctc caattgtccc tcatatctcc tcctccaggt ctgaagatca gcggccggcc
      241 gcttgctgtg cggtggtctt acttttgttt tgctcttcct ctatcttgtc taaagcttcc
      301 ttggtgtctt ttatctctat cctttgatgc acacaataga gggttgctac tgtattatat
      361 aatgatctaa gttcttctga tcctgtctga agggatggtt gtagctgtcc cagtatttgt
      421 ctacagcctt ctgatgtttc taacaggcca ggattaactg cgaatcgttc tagctccctg
      481 cttgcccata ctatatgttt taatttatat tttttctttc cccctggcct taaccgaatt
      541 ttttcccatc gcgatctaat tctcccccgc ttaatactga cgctctcgca cccatctctc
      601 tccttctagc ctccgctagt caaaattttt ggcgtactca ccagtcgccg cccctcgcct
      661 cttgccgtgc gcgcttcagc aagccgagtc ctgcgtcgag agagctcctc tggtttccct
      721 ttcgctttca agtccctgtt cgggcgccac tgctagagat tttccacact gactaaaagg
      781 gtctgaggga tctctagtta ccagagtcac acaacagacg ggcacacact acttgaagca
      841 ctcaaggcaa gctttattga ggcttaagca gtgggttccc tagttagcca gagagctccc
      901 aggctcagat ctggtctaac cagagagacc cagtacaggc aaaacgcgct gcttatatag
      961 acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggagtt gttacgacat
     1021 tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc aatggggtgg
     1081 agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac
     1141 cgcatcacca tggtaatagc gatgactaat acgtagatgt actgccaagt aggaaagtcc
     1201 cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt gacgtcaata
     1261 gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt ttaccgtaaa
     1321 tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg aacatacgtc
     1381 attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc atttaccgta
     1441 agttatgtaa cgcggaactc catatatggg ctatgaacta atgaccccgt aattgattac
     1501 tattaataac tagtcaataa tcaatgtcaa cgcgtatatc tggcccgtac atcgcgaagc
     1561 agcgcaaaac gcctaaccct aagcagattc ttcatgcaat tgtcggtcaa gccttgcctt
     1621 gttgtagctt aaattttgct cgcgcactac tcagcgacct ccaacacaca agcagggagc
     1681 agatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatagggga
     1741 tcgggagatc tcccgatccg tcgacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     1801 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     1861 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     1921 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     1981 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     2041 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     2101 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     2161 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     2221 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     2281 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     2341 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     2401 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     2461 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     2521 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     2581 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     2641 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     2701 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     2761 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     2821 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     2881 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     2941 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     3001 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     3061 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     3121 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     3181 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     3241 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     3301 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     3361 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     3421 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     3481 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     3541 tacggttcct ggccttttgc tggccttttg ctcacatgct gctagagatt ttccacactg
     3601 actaaaaggg tctgagggat ctctagttac cagagtcaca caacagacgg gcacacacta
     3661 cttgaagcac tcaaggcaag ctttattgag gcttaagcag tgggttccct agttagccag
     3721 agagctccca ggctcagatc tggtctaacc agagagaccc agtacagtcc ggatgcagct
     3781 ctcgggccat gtgatgaaat gctaggcggc tgtcaaacct ccactctaat acttctctct
     3841 ccgggtcatc catcccatgc aggctcacag ggtgtaacaa gcgggtgttc tctccttcat
     3901 tggcttcttc taccttctct tgctcaactg gtactagctt gtagcaccat ccaaaggtca
     3961 gtggatatct gatccctggc cctggtgtgt agttctgcca atcagggaag tagccttgtg
     4021 tgtggtagat ccacagatca aggatatctt gtcttcgttg ggagtgaatt agcccttcca
     4081 gtcccccctt ttcttttaaa aagtggctaa gatctacagc tgccttgtaa gtcattggtc
     4141 ttaaaggtac tacatcgtag atcagctcga cattgattat tgactagtta ttaatagtaa
     4201 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
     4261 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     4321 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggactattta
     4381 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
     4441 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
     4501 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggg tcgaggtgag
     4561 ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa ttttgtattt
     4621 atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg cgcgcgccag
     4681 gcggggcggg gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca
     4741 atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct
     4801 ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgtt gccttcgccc cgtgccccgc
     4861 tccgcgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga
     4921 gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggctcg
     4981 tttcttttct gtggctgcgt gaaagcctta aagggctccg ggagggccct ttgtgcgggg
     5041 gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggcccg
     5101 cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg ctccgcgtgt
     5161 gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc gggggggctg cgaggggaac
     5221 aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg cgcggcggtc
     5281 gggctgtaac ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg
     5341 tgcggggctc cgtgcggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca
     5401 ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg gggaggggcg
     5461 cggcggcccc ggagcgccgg cggctgtcga ggcgcggcga gccgcagcca ttgcctttta
     5521 tggtaatcgt gcgagagggc gcagggactt cctttgtccc aaatctggcg gagccgaaat
     5581 ctgggaggcg ccgccgcacc ccctctagcg ggcgcgggcg aagcggtgcg gcgccggcag
     5641 gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc ttctccatct
     5701 ccagcctcgg ggctgccgca gggggacggc tgccttcggg ggggacgggg cagggcgggg
     5761 ttcggcttct ggcgtgtgac cggcggctct agagcctctg ctaaccatgt tcatgccttc
     5821 ttctttttcc tacagctcct gctagtcgta actagcgcta ccggtcgcca ccatggtgag
     5881 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     5941 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     6001 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     6061 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
     6121 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     6181 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     6241 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     6301 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     6361 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     6421 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     6481 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     6541 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagg ggtccgggga
     6601 agggcgcggt tccttgctta cgtgtggtga cgtggaggag aatcctgggc ctgaattcat
     6661 gcaggaatct gcgacagaga caataagcaa cagttcaatg aatcaaaatg gaatgagcac
     6721 tctaagcagc caattagatg ctggcagcag agatggaaga tcaagtggtg acaccagctc
     6781 tgaagtaagc acagtagaac tgctgcatct gcaacaacag caggctctcc aggcagcaag
     6841 acaacttctt ttacagcagc aaacaagtgg attgaaatct cctaagagca gtgataaaca
     6901 gagaccactg caggaattgc ttccagaaac aaaattatgt atctgtggcc actcttctgg
     6961 tgatgggcat cctcacaaca catttgcagt gcctgtgtca gtggccatga tgactcccca
     7021 ggtgatcacc cctcagcaaa tgcagcagat ccttcagcaa caagtcctgt ctcctcagca
     7081 gctacaagcc cttctccaac aacagcaggc tgtcatgctg cagcagcaac aactacaaga
     7141 gttttacaag aaacagcaag agcagttaca tcttcagctt ttgcagcagc agcagcaaca
     7201 gcagcagcag caacaacagc agcaacaaca gcagcagcaa caacaacaac aacagcagca
     7261 acaacagcag cagcagcagc aacagcagca gcagcagcaa cagcatcctg gaaagcaagc
     7321 gaaagagcag cagcagcagc agcagcagca acagcaattg gcagcccagc agcttgtctt
     7381 ccagcagcag cttctccaga tgcaacaact ccagcagcag cagcatctgc tcagccttca
     7441 gcgtcaggga ctcatctcca ttccacctgg ccaggcagca cttcctgtcc aatcgctgcc
     7501 tcaagctggc ttaagtcctg ctgagattca gcagttatgg aaagaagtga ctggagttca
     7561 cagtatggaa gacaatggca ttaaacatgg agggctagac ctcactacta acaattcctc
     7621 ctcgactacc tcctccaaca cttccaaagc atcaccacca ataactcatc attccatagt
     7681 gaatggacag tcttcagttc taagtgcaag acgagacagc tcgtcacatg aggagactgg
     7741 ggcctctcac actctctatg gccatggagt ttgcaaatgg ccaggctgtg aaagcatttg
     7801 tgaagatttt ggacagtttt taaagcacct taacaatgaa cacgcattgg atgaccgaag
     7861 cactgctcag tgtcgagtgc aaatgcaggt ggtgcaacag ttagaaatac agctttctaa
     7921 agaacgcgaa cgtcttcaag caatgatgac ccacttgcac atgcgaccct cagagcccaa
     7981 accatctccc aaacctctaa atctggtgtc tagtgtcacc atgtcgaaga atatgttgga
     8041 gacatcccca cagagcttac ctcaaacccc taccacacca acggccccag tcaccccgat
     8101 tacccaggga ccctcagtaa tcaccccagc cagtgtgccc aatgtgggag ccatacgaag
     8161 gcgacattca gacaaataca acattcccat gtcatcagaa attgccccaa actatgaatt
     8221 ttataaaaat gcagatgtca gacctccatt tacttatgca actctcataa ggcaggctat
     8281 catggagtca tctgacaggc agttaacact taatgaaatt tacagctggt ttacacggac
     8341 atttgcttac ttcaggcgta atgcagcaac ttggaagaat gcagtacgtc ataatcttag
     8401 cctgcacaag tgttttgttc gagtagaaaa tgttaaagga gcagtatgga ctgtggatga
     8461 agtagaatac cagaagcgaa ggtcacaaaa gataacagga agtccaacct tagtaaaaaa
     8521 tatacctacc agtttaggct atggagcagc tcttaatgcc agtttgcagg ctgccttggc
     8581 agagagcagt ttacctttgc taagtaatcc tggactgata aataatgcat ccagtggcct
     8641 actgcaggcc gtccacgaag acctcaatgg ttctctggat cacattgaca gcaatggaaa
     8701 cagtagtccg ggctgctcac ctcagccgca catacattca atccacgtca aggaagagcc
     8761 agtgattgca gaggatgaag actgcccaat gtccttagtg acaacagcta atcacagtcc
     8821 agaattagaa gacgacagag agattgaaga agagccttta tctgaagatc tggaatgagg
     8881 ccgctacaaa gcttatgccg acgtacgatc caatcaacct ctggattaca aaatttgtga
     8941 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     9001 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     9061 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     9121 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     9181 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     9241 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     9301 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     9361 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     9421 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     9481 cctttgggcc gcctccccgc gtcctggacc tccctagcaa actggggcac aagcgaatta
     9541 tcgaattccc acctaggtag ggatccaagg gtaccttcgg cgcgccgtat gatcattggc
     9601 tagctcgaca tgtctactcg agctgtgcct tctagttgcc agccatctgt tgtttgcccc
     9661 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     9721 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     9781 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     9841 tctatggcta gagcgggccc ggtactaacc aaactggatc tctgctgtcc ctgtaataaa
     9901 cccgaaaatt ttgaattttt gtaatttgtt tttgtaattc tttagtttgt atgtctgttg
     9961 ctattatgtc tactattctt tcccctgcac tgtacccccc aatcccccct tttcttttaa
    10021 aattgtggat gaatactgcc atttgtctgc agaattggcg cacgcagtgc cgatccgttc
    10081 actaatcgaa tggatctgtc tctgtctctc tctccacctt cttcttctat tccttcgggc
    10141 ctgtcgggtc ccctcggggt tgggaggtgg gtctgaaacg ataatggtga atatccctgc
    10201 ctaactctat tcactataga aagtacagca aaaactattc ttaaacctac caagcctcct
    10261 actatcatta tgaataattt tatataccac agccaatttg ttatgttaaa ccaattccac
    10321 aaacttgccc atttatctaa ttccaataat tcttgttcat tcttttcttg ctggttttgc
    10381 gattcttcaa ttaaggagtg tattaagctt gtgtaattgt taatttctct gtcccactcc
    10441 atccaggtcg tgtgattcca aatctgttcc agagatttat tactccaact agcattccaa
    10501 ggcacagcag tggtgcaaat gagttttcca gagcaacccc aaatccccag gagctgttga
    10561 tcctttaggt atctttccac agccaggatt cttgcctgga gctgcttgat gccccagact
    10621 gtgagttgca acagatgctg ttgcgcctca atagccctca gcaaattgtt ctgctgctgc
    10681 actataccag acaataattg
//
