LOCUS       Exported               10802 bp ds-DNA     circular SYN 07-JUN-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 10802)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Dec 9, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10802
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    579..704
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             751..931
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(949..1147)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        1149..1528
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     1700..1719
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        1794..1898
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1899..2759
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2117..2136)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2930..3518
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3419..3438
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             3580..3760
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3761..4080
                     /label=U3
     misc_feature    4171..4183
                     /label=CMV_enhancer
     enhancer        4184..4548
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4551..4826
                     /label=chicken beta-actin promoter
     primer_bind     complement(4662..4671)
                     /label=Oligo_131
     primer_bind     complement(4705..4785)
                     /label=Oligo_131
     intron          4827..5788
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     misc_feature    5789..5835
                     /label=chimeric intro
     primer_bind     5836..5857
                     /label=Primer 6
     primer_bind     5849..5873
                     /label=AgeI-F
     primer_bind     5867..5884
                     /label=dualpromoter-F5v2
                     /note="tctaga : XbaI site, destroy AgeI here."
     CDS             5873..6589
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     6284..6295
                     /label=Oligo_19
     misc_feature    6559
                     /label=A?
     misc_feature    6599..6652
                     /label=T2A
     primer_bind     6628..6675
                     /label=Primer 1
     primer_bind     complement(6633..6653)
                     /label=dualpromoter-R5v2
     primer_bind     complement(6633..6652)
                     /label=Primer 5
     CDS             6659..8980
                     /codon_start=1
                     /label=DGCR8
                     /translation="METDESPSPLPCGPAGEAVMESRARPFQALPREQSPPPPLQTSSG
                     AEVMDVGSGGDGQSELPAEDPFNFYGASLLSKGSFSKGRLLIDPNCSGHSPRTARHAPA
                     VRKFSPDLKLLKDVKISVSFTESCRSKDRKVLYTGAERDVRAECGLLLSPVSGDVHACP
                     FGGSVGDGVGIGGESADKKDEENELDQEKRVEYAVLDELEDFTDNLELDEEGAGGFTAK
                     AIVQRDRVDEEALNFPYEDDFDNDVDALLEEGLCAPKKRRTEEKYGGDSDHPSDGETSV
                     QPMMTKIKTVLKSRGRPPTEPLPDGWIMTFHNSGVPVYLHRESRVVTWSRPYFLGTGSI
                     RKHDPPLSSIPCLHYKKMKDNEEREQSSDLTPSGDVSPVKPLSRSAELEFPLDEPDSMG
                     ADPGPPDEKDPLGAEAAPGALGQVKAKVEVCKDESVDLEEFRSYLEKRFDFEQVTVKKF
                     RTWAERRQFNREMKRKQAESERPILPANQKLITLSVQDAPTKKEFVINPNGKSEVCILH
                     EYMQRVLKVRPVYNFFECENPSEPFGASVTIDGVTYGSGTASSKKLAKNKAARATLEIL
                     IPDFVKQTSEEKPKDSEELEYFNHISIEDSRVYELTSKAGLLSPYQILHECLKRNHGMG
                     DTSIKFEVVPGKNQKSEYVMACGKHTVRGWCKNKRVGKQLASQKILQLLHPHVKNWGSL
                     LRMYGRESSKMVKQETSDKSVIELQQYAKKNKPNLHILSKLQEEMKRLAEEREETRKKP
                     KMSIVASAQPGGEPLCTVDV"
     primer_bind     complement(6905..6914)
                     /label=Oligo_42
     primer_bind     complement(6905..6914)
                     /label=Oligo_41
     primer_bind     complement(8961..9005)
                     /label=Primer 3
     primer_bind     complement(8961..8980)
                     /label=Primer 2
     primer_bind     8981..9025
                     /label=Oligo_40
     primer_bind     9001..9025
                     /label=Primer 2
     misc_feature    9014..9602
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9067..9087)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9485..9496)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9589..9607)
                     /label=Oligo_41
     primer_bind     complement(9589..9603)
                     /label=Oligo_42
     primer_bind     complement(9594..9603)
                     /label=Primer 1
     primer_bind     9604..9635
                     /label=Oligo_19
     misc_feature    9616
                     /label=GFP homology
     misc_feature    9617..9636
                     /label=BFP_Perturb_Primer
     misc_feature    9637
                     /label=Linker
     misc_feature    9724
                     /label=Barcode_Cloning_Site
     polyA_signal    9725..9921
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     9910..9932
                     /label=Primer 4
     misc_feature    9922..9949
                     /label=bGH
     misc_feature    10007..10124
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    join(10651..10802,1..82)
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 tctggcctgt accgtcagcg tcattgacgc tgcgcccata gtgcttcctg ctgctcccaa
       61 gaacccaagg aacaaagctc ctattcccac tgctcttttt tctctctgca ccactcttct
      121 ctttgccttg gtgggtgcta ctcctaatgg ttcaattttt actactttat atttatataa
      181 ttcacttctc caattgtccc tcatatctcc tcctccaggt ctgaagatca gcggccggcc
      241 gcttgctgtg cggtggtctt acttttgttt tgctcttcct ctatcttgtc taaagcttcc
      301 ttggtgtctt ttatctctat cctttgatgc acacaataga gggttgctac tgtattatat
      361 aatgatctaa gttcttctga tcctgtctga agggatggtt gtagctgtcc cagtatttgt
      421 ctacagcctt ctgatgtttc taacaggcca ggattaactg cgaatcgttc tagctccctg
      481 cttgcccata ctatatgttt taatttatat tttttctttc cccctggcct taaccgaatt
      541 ttttcccatc gcgatctaat tctcccccgc ttaatactga cgctctcgca cccatctctc
      601 tccttctagc ctccgctagt caaaattttt ggcgtactca ccagtcgccg cccctcgcct
      661 cttgccgtgc gcgcttcagc aagccgagtc ctgcgtcgag agagctcctc tggtttccct
      721 ttcgctttca agtccctgtt cgggcgccac tgctagagat tttccacact gactaaaagg
      781 gtctgaggga tctctagtta ccagagtcac acaacagacg ggcacacact acttgaagca
      841 ctcaaggcaa gctttattga ggcttaagca gtgggttccc tagttagcca gagagctccc
      901 aggctcagat ctggtctaac cagagagacc cagtacaggc aaaacgcgct gcttatatag
      961 acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggagtt gttacgacat
     1021 tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc aatggggtgg
     1081 agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac
     1141 cgcatcacca tggtaatagc gatgactaat acgtagatgt actgccaagt aggaaagtcc
     1201 cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt gacgtcaata
     1261 gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt ttaccgtaaa
     1321 tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg aacatacgtc
     1381 attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc atttaccgta
     1441 agttatgtaa cgcggaactc catatatggg ctatgaacta atgaccccgt aattgattac
     1501 tattaataac tagtcaataa tcaatgtcaa cgcgtatatc tggcccgtac atcgcgaagc
     1561 agcgcaaaac gcctaaccct aagcagattc ttcatgcaat tgtcggtcaa gccttgcctt
     1621 gttgtagctt aaattttgct cgcgcactac tcagcgacct ccaacacaca agcagggagc
     1681 agatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatagggga
     1741 tcgggagatc tcccgatccg tcgacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     1801 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     1861 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     1921 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     1981 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     2041 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     2101 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     2161 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     2221 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     2281 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     2341 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     2401 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     2461 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     2521 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     2581 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     2641 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     2701 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     2761 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     2821 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     2881 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     2941 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     3001 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     3061 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     3121 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     3181 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     3241 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     3301 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     3361 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     3421 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     3481 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     3541 tacggttcct ggccttttgc tggccttttg ctcacatgct gctagagatt ttccacactg
     3601 actaaaaggg tctgagggat ctctagttac cagagtcaca caacagacgg gcacacacta
     3661 cttgaagcac tcaaggcaag ctttattgag gcttaagcag tgggttccct agttagccag
     3721 agagctccca ggctcagatc tggtctaacc agagagaccc agtacagtcc ggatgcagct
     3781 ctcgggccat gtgatgaaat gctaggcggc tgtcaaacct ccactctaat acttctctct
     3841 ccgggtcatc catcccatgc aggctcacag ggtgtaacaa gcgggtgttc tctccttcat
     3901 tggcttcttc taccttctct tgctcaactg gtactagctt gtagcaccat ccaaaggtca
     3961 gtggatatct gatccctggc cctggtgtgt agttctgcca atcagggaag tagccttgtg
     4021 tgtggtagat ccacagatca aggatatctt gtcttcgttg ggagtgaatt agcccttcca
     4081 gtcccccctt ttcttttaaa aagtggctaa gatctacagc tgccttgtaa gtcattggtc
     4141 ttaaaggtac tacatcgtag atcagctcga cattgattat tgactagtta ttaatagtaa
     4201 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
     4261 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     4321 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggactattta
     4381 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
     4441 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
     4501 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggg tcgaggtgag
     4561 ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa ttttgtattt
     4621 atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg cgcgcgccag
     4681 gcggggcggg gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca
     4741 atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct
     4801 ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgtt gccttcgccc cgtgccccgc
     4861 tccgcgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga
     4921 gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggctcg
     4981 tttcttttct gtggctgcgt gaaagcctta aagggctccg ggagggccct ttgtgcgggg
     5041 gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggcccg
     5101 cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg ctccgcgtgt
     5161 gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc gggggggctg cgaggggaac
     5221 aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg cgcggcggtc
     5281 gggctgtaac ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg
     5341 tgcggggctc cgtgcggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca
     5401 ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg gggaggggcg
     5461 cggcggcccc ggagcgccgg cggctgtcga ggcgcggcga gccgcagcca ttgcctttta
     5521 tggtaatcgt gcgagagggc gcagggactt cctttgtccc aaatctggcg gagccgaaat
     5581 ctgggaggcg ccgccgcacc ccctctagcg ggcgcgggcg aagcggtgcg gcgccggcag
     5641 gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc ttctccatct
     5701 ccagcctcgg ggctgccgca gggggacggc tgccttcggg ggggacgggg cagggcgggg
     5761 ttcggcttct ggcgtgtgac cggcggctct agagcctctg ctaaccatgt tcatgccttc
     5821 ttctttttcc tacagctcct gctagtcgta actagcgcta ccggtcgcca ccatggtgag
     5881 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     5941 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     6001 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     6061 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
     6121 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     6181 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     6241 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     6301 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     6361 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     6421 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     6481 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     6541 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagg ggtccgggga
     6601 agggcgcggt tccttgctta cgtgtggtga cgtggaggag aatcctgggc ctgtcgacat
     6661 ggagacagat gagagcccct ctccgctccc gtgtgggccc gcaggagaag cggtgatgga
     6721 gagccgagct cgccccttcc aagcgctgcc ccgtgagcag tctccaccac ctcccctgca
     6781 aacgtccagt ggtgcagagg taatggacgt tggctctggt ggtgatggac agtccgaact
     6841 ccctgctgag gaccccttca acttctacgg agcttctctt ctctccaaag gatccttctc
     6901 taagggccgc ctcctcatag acccgaactg tagtggccac agcccgcgca ccgcccggca
     6961 cgcacctgcg gtccggaagt tctcccctga ccttaagttg cttaaggatg taaagattag
     7021 cgtgagcttt accgagagct gcaggagtaa ggacaggaag gtgctgtaca caggagcaga
     7081 gcgcgacgtg cgggcggagt gcggtctgct ccttagccct gtcagtgggg acgtgcatgc
     7141 ttgtcccttt ggcgggagtg ttggtgacgg ggtaggcata gggggtgaga gtgctgataa
     7201 gaaggatgag gagaatgagc tggatcagga aaagagagtg gagtatgcag tgctcgatga
     7261 gttagaagat tttactgaca atttggagct agatgaagaa ggagcaggcg ggttcacggc
     7321 taaagcaatc gttcagagag acagagtgga tgaagaggcc ttgaatttcc cctacgagga
     7381 tgactttgac aacgatgtgg atgctctgct ggaagaaggc ctttgtgccc ccaaaaagag
     7441 gcgaacagag gaaaaatatg gcggagacag cgaccatccg tccgatggag agacaagtgt
     7501 gcagccgatg atgaccaaga ttaaaacagt gctcaaaagt cgtggccgcc cacctacaga
     7561 gccgctgccc gacgggtgga tcatgacatt ccataactct ggagtcccgg tgtacctaca
     7621 cagagagtct cgggtggtca cctggtccag gccatacttc ttgggaacgg gaagcatacg
     7681 gaaacacgac cctcctctga gtagcatccc ttgtctgcat tataagaaaa tgaaggacaa
     7741 cgaggaacgg gagcaaagca gtgacctcac ccctagtggg gatgtgtccc ccgtcaagcc
     7801 cctgagccga tctgcagagc tggagtttcc cctggatgag cctgactcta tgggtgctga
     7861 cccggggccc ccggacgaga aagacccact aggggctgag gcagcccctg gggccctggg
     7921 gcaggtgaag gccaaagtcg aggtgtgcaa agatgaatcc gttgatctcg aggaatttcg
     7981 aagctacctg gagaagcgtt ttgactttga gcaagttact gtgaaaaaat tcaggacttg
     8041 ggctgagcgg cggcaattca atcgggaaat gaagcggaag caggcggagt ccgagaggcc
     8101 catcttgcca gccaatcaga agctcattac tttatcagtg caagatgcac ccacaaagaa
     8161 agagtttgtt attaacccca acgggaaatc cgaggtctgc atcctgcacg agtacatgca
     8221 gcgtgtcctc aaggtccgcc ctgtctataa tttctttgaa tgtgagaacc caagtgagcc
     8281 ttttggtgcc tcggtgacca ttgatggtgt gacttacgga tctggaactg caagcagcaa
     8341 aaaacttgcg aagaataaag ctgcccgagc tacactggaa atcctcatcc ctgactttgt
     8401 taaacagacc tctgaagaga agcccaaaga cagtgaagaa ctcgagtatt ttaaccacat
     8461 cagcatcgag gactcgcggg tctacgagct gaccagcaag gctgggctgt tgtctccata
     8521 tcagatcctc cacgagtgcc ttaaaagaaa ccatgggatg ggtgacacgt ctatcaagtt
     8581 tgaagtggtt cctgggaaaa accagaagag tgaatacgtc atggcgtgtg gcaagcacac
     8641 agtgcgcggg tggtgtaaga acaagagagt tggaaagcag ttagcctcac agaagatcct
     8701 tcagctgctg cacccacatg tcaagaactg ggggtcttta ctgcgcatgt atggccgtga
     8761 gagcagcaag atggtcaagc aggagacatc ggacaagagt gtgattgagc tgcagcagta
     8821 tgccaagaag aacaagccca acctgcacat cctcagcaag ctccaagagg agatgaagag
     8881 gctagctgag gaaagggagg agactcgaaa gaagcccaag atgtccattg tggcgtccgc
     8941 ccagcctggc ggtgagcccc tgtgcaccgt ggacgtgtga ggccgctaca aagcttatgc
     9001 cgacgtacga tccaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     9061 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     9121 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     9181 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     9241 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     9301 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     9361 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     9421 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     9481 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     9541 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     9601 gcgtcctgga cctccctagc aaactggggc acaagcgaat tatcgaattc ccacctaggt
     9661 agggatccaa gggtaccttc ggcgcgccgt atgatcattg gctagctcga catgtctact
     9721 cgagctgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     9781 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     9841 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     9901 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc tagagcgggc
     9961 ccggtactaa ccaaactgga tctctgctgt ccctgtaata aacccgaaaa ttttgaattt
    10021 ttgtaatttg tttttgtaat tctttagttt gtatgtctgt tgctattatg tctactattc
    10081 tttcccctgc actgtacccc ccaatccccc cttttctttt aaaattgtgg atgaatactg
    10141 ccatttgtct gcagaattgg cgcacgcagt gccgatccgt tcactaatcg aatggatctg
    10201 tctctgtctc tctctccacc ttcttcttct attccttcgg gcctgtcggg tcccctcggg
    10261 gttgggaggt gggtctgaaa cgataatggt gaatatccct gcctaactct attcactata
    10321 gaaagtacag caaaaactat tcttaaacct accaagcctc ctactatcat tatgaataat
    10381 tttatatacc acagccaatt tgttatgtta aaccaattcc acaaacttgc ccatttatct
    10441 aattccaata attcttgttc attcttttct tgctggtttt gcgattcttc aattaaggag
    10501 tgtattaagc ttgtgtaatt gttaatttct ctgtcccact ccatccaggt cgtgtgattc
    10561 caaatctgtt ccagagattt attactccaa ctagcattcc aaggcacagc agtggtgcaa
    10621 atgagttttc cagagcaacc ccaaatcccc aggagctgtt gatcctttag gtatctttcc
    10681 acagccagga ttcttgcctg gagctgcttg atgccccaga ctgtgagttg caacagatgc
    10741 tgttgcgcct caatagccct cagcaaattg ttctgctgct gcactatacc agacaataat
    10801 tg
//
