LOCUS       Exported               14986 bp ds-DNA     circular SYN 09-DEC-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14986)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Dec 9, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14986
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2364..2397
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_RNA        complement(2408..2479)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(2512..2752)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     primer_bind     complement(2562..2581)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(2732..2752)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        2785..3996
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3833..3852
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      4025..4034
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             4034..4099
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             4106..4126
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4136..8239
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             8252..8272
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8306..8500
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQILYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     CDS             8567..8587
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8594..8647
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             8654..9367
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             8654..9367
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(8696..8717)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8957..8976)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9304..9325
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    9383..9416
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    9441..10029
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9494..9514)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9912..9923)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10032..10048)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10033..10049)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             10554..10734
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10760..10777)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10766..10990
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      11036..11464
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11123..11142)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11333..11354
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(11473..11493)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11478..11807
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11658..11793
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     11720..11739
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        11855..11902
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             11921..12295
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    12425..12546
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12462..12481)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12516..12535
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(12595..12611)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12595..12611)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12608..12630)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12619..12635
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(12643..12673)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12688..12709
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12826..12843)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12997..13585)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13077..13096)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(13756..14616)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14379..14398
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14617..14721)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(14796..14815)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttaa ttaataactt cgtatagcat acattatacg aagttattaa
     2401 aaaaaaagca ccgactcggt gccacttttt caagttgata acggactagc cttattttaa
     2461 cttgctattt ctagctctaa aacgtgtctg gtctcccggc tccggtgttt cgtcctttcc
     2521 acaagatata taaagccaag aaatcgaaat actttcaagt tacggtaagc atatgatagt
     2581 ccattttaaa acataatttt aaaactgcaa actacccaag aaattattac tttctacgtc
     2641 acgtattttg tactaatatc tttgtgttta cagtcaaatt aattccaatt atctctctaa
     2701 cagccttgta tcgtatatgc aaatatgaag gaatcatggg aaataggccc tcttaattaa
     2761 ttaacccgtg tcggctccag atctggcctc cgcgccgggt tttggcgcct cccgcgggcg
     2821 cccccctcct cacggcgagc gctgccacgt cagacgaagg gcgcagcgag cgtcctgatc
     2881 cttccgcccg gacgctcagg acagcggccc gctgctcata agactcggcc ttagaacccc
     2941 agtatcagca gaaggacatt ttaggacggg acttgggtga ctctagggca ctggttttct
     3001 ttccagagag cggaacaggc gaggaaaagt agtcccttct cggcgattct gcggagggat
     3061 ctccgtgggg cggtgaacgc cgatgattat ataaggacgc gccgggtgtg gcacagctag
     3121 ttccgtcgca gccgggattt gggtcgcggt tcttgtttgt ggatcgctgt gatcgtcact
     3181 tggtgagtag cgggctgctg ggctggccgg ggctttcgtg gccgccgggc cgctcggtgg
     3241 gacggaagcg tgtggagaga ccgccaaggg ctgtagtctg ggtccgcgag caaggttgcc
     3301 ctgaactggg ggttgggggg agcgcagcaa aatggcggct gttcccgagt cttgaatgga
     3361 agacgcttgt gaggcgggct gtgaggtcgt tgaaacaagg tggggggcat ggtgggcggc
     3421 aagaacccaa ggtcttgagg ccttcgctaa tgcgggaaag ctcttattcg ggtgagatgg
     3481 gctggggcac catctgggga ccctgacgtg aagtttgtca ctgactggag aactcggttt
     3541 gtcgtctgtt gcgggggcgg cagttatggc ggtgccgttg ggcagtgcac ccgtaccttt
     3601 gggagcgcgc gccctcgtcg tgtcgtgacg tcacccgttc tgttggctta taatgcaggg
     3661 tggggccacc tgccggtagg tgtgcggtag gcttttctcc gtcgcaggac gcagggttcg
     3721 ggcctagggt aggctctcct gaatcgacag gcgccggacc tctggtgagg ggagggataa
     3781 gtgaggcgtc agtttctttg gtcggtttta tgtacctatc ttcttaagta gctgaagctc
     3841 cggttttgaa ctatgcgctc ggggttggcg agtgtgtttt gtgaagtttt ttaggcacct
     3901 tttgaaatgt aatcatttgg gtcaatatgt aattttcagt gttagactag taaattgtcc
     3961 gctaaattct ggccgttttt ggcttttttg ttagacgaag cttgggctgc aggtcgactc
     4021 tagagccacc atggactaca aagaccatga cggtgattat aaagatcatg acatcgatta
     4081 caaggatgac gatgacaaga tggcccccaa gaagaagagg aaggtgggcc gcggaatgga
     4141 caagaagtac tccattgggc tcgccatcgg cacaaacagc gtcggctggg ccgtcattac
     4201 ggacgagtac aaggtgccga gcaaaaaatt caaagttctg ggcaataccg atcgccacag
     4261 cataaagaag aacctcattg gcgccctcct gttcgactcc ggggaaaccg ccgaagccac
     4321 gcggctcaaa agaacagcac ggcgcagata tacccgcaga aagaatcgga tctgctacct
     4381 gcaggagatc tttagtaatg agatggctaa ggtggatgac tctttcttcc ataggctgga
     4441 ggagtccttt ttggtggagg aggataaaaa gcacgagcgc cacccaatct ttggcaatat
     4501 cgtggacgag gtggcgtacc atgaaaagta cccaaccata tatcatctga ggaagaagct
     4561 tgtagacagt actgataagg ctgacttgcg gttgatctat ctcgcgctgg cgcatatgat
     4621 caaatttcgg ggacacttcc tcatcgaggg ggacctgaac ccagacaaca gcgatgtcga
     4681 caaactcttt atccaactgg ttcagactta caatcagctt ttcgaagaga acccgatcaa
     4741 cgcatccgga gttgacgcca aagcaatcct gagcgctagg ctgtccaaat cccggcggct
     4801 cgaaaacctc atcgcacagc tccctgggga gaagaagaac ggcctgtttg gtaatcttat
     4861 cgccctgtca ctcgggctga cccccaactt taaatctaac ttcgacctgg ccgaagatgc
     4921 caagcttcaa ctgagcaaag acacctacga tgatgatctc gacaatctgc tggcccagat
     4981 cggcgaccag tacgcagacc tttttttggc ggcaaagaac ctgtcagacg ccattctgct
     5041 gagtgatatt ctgcgagtga acacggagat caccaaagct ccgctgagcg ctagtatgat
     5101 caagcgctat gatgagcacc accaagactt gactttgctg aaggcccttg tcagacagca
     5161 actgcctgag aagtacaagg aaattttctt cgatcagtct aaaaatggct acgccggata
     5221 cattgacggc ggagcaagcc aggaggaatt ttacaaattt attaagccca tcttggaaaa
     5281 aatggacggc accgaggagc tgctggtaaa gcttaacaga gaagatctgt tgcgcaaaca
     5341 gcgcactttc gacaatggaa gcatccccca ccagattcac ctgggcgaac tgcacgctat
     5401 cctcaggcgg caagaggatt tctacccctt tttgaaagat aacagggaaa agattgagaa
     5461 aatcctcaca tttcggatac cctactatgt aggccccctc gcccggggaa attccagatt
     5521 cgcgtggatg actcgcaaat cagaagagac catcactccc tggaacttcg aggaagtcgt
     5581 ggataagggg gcctctgccc agtccttcat cgaaaggatg actaactttg ataaaaatct
     5641 gcctaacgaa aaggtgcttc ctaaacactc tctgctgtac gagtacttca cagtttataa
     5701 cgagctcacc aaggtcaaat acgtcacaga agggatgaga aagccagcat tcctgtctgg
     5761 agagcagaag aaagctatcg tggacctcct cttcaagacg aaccggaaag ttaccgtgaa
     5821 acagctcaaa gaagactatt tcaaaaagat tgaatgtttc gactctgttg aaatcagcgg
     5881 agtggaggat cgcttcaacg catccctggg aacgtatcac gatctcctga aaatcattaa
     5941 agacaaggac ttcctggaca atgaggagaa cgaggacatt cttgaggaca ttgtcctcac
     6001 ccttacgttg tttgaagata gggagatgat tgaagaacgc ttgaaaactt acgctcatct
     6061 cttcgacgac aaagtcatga aacagctcaa gaggcgccga tatacaggat gggggcggct
     6121 gtcaagaaaa ctgatcaatg ggatccgaga caagcagagt ggaaagacaa tcctggattt
     6181 tcttaagtcc gatggatttg ccaaccggaa cttcatgcag ttgatccatg atgactctct
     6241 cacctttaag gaggacatcc agaaagcaca agtttctggc cagggggaca gtcttcacga
     6301 gcacatcgct aatcttgcag gtagcccagc tatcaaaaag ggaatactgc agaccgttaa
     6361 ggtcgtggat gaactcgtca aagtaatggg aaggcataag cccgagaata tcgttatcga
     6421 gatggcccga gagaaccaaa ctacccagaa gggacagaag aacagtaggg aaaggatgaa
     6481 gaggattgaa gagggtataa aagaactggg gtcccaaatc cttaaggaac acccagttga
     6541 aaacacccag cttcagaatg agaagctcta cctgtactac ctgcagaacg gcagggacat
     6601 gtacgtggat caggaactgg acatcaatcg gctctccgac tacgacgtgg atgccatcgt
     6661 gccccagtct tttctcaaag atgattctat tgataataaa gtgttgacaa gatccgataa
     6721 aaatagaggg aagagtgata acgtcccctc agaagaagtt gtcaagaaaa tgaaaaatta
     6781 ttggcggcag ctgctgaacg ccaaactgat cacacaacgg aagttcgata atctgactaa
     6841 ggctgaacga ggtggcctgt ctgagttgga taaagccggc ttcatcaaaa ggcagcttgt
     6901 tgagacacgc cagatcacca agcacgtggc ccaaattctc gattcacgca tgaacaccaa
     6961 gtacgatgaa aatgacaaac tgattcgaga ggtgaaagtt attactctga agtctaagct
     7021 ggtctcagat ttcagaaagg actttcagtt ttataaggtg agagagatca acaattacca
     7081 ccatgcgcat gatgcctacc tgaatgcagt ggtaggcact gcacttatca aaaaatatcc
     7141 caagcttgaa tctgaatttg tttacggaga ctataaagtg tacgatgtta ggaaaatgat
     7201 cgcaaagtct gagcaggaaa taggcaaggc caccgctaag tacttctttt acagcaatat
     7261 tatgaatttt ttcaagaccg agattacact ggccaatgga gagattcgga agcgaccact
     7321 tatcgaaaca aacggagaaa caggagaaat cgtgtgggac aagggtaggg atttcgcgac
     7381 agtccggaag gtcctgtcca tgccgcaggt gaacatcgtt aaaaagaccg aagtacagac
     7441 cggaggcttc tccaaggaaa gtatcctccc gaaaaggaac agcgacaagc tgatcgcacg
     7501 caaaaaagat tgggacccca agaaatacgg cggattcgat tctcctacag tcgcttacag
     7561 tgtactggtt gtggccaaag tggagaaagg gaagtctaaa aaactcaaaa gcgtcaagga
     7621 actgctgggc atcacaatca tggagcgatc aagcttcgaa aaaaacccca tcgactttct
     7681 cgaggcgaaa ggatataaag aggtcaaaaa agacctcatc attaagcttc ccaagtactc
     7741 tctctttgag cttgaaaacg gccggaaacg aatgctcgct agtgcgggcg agctgcagaa
     7801 aggtaacgag ctggcactgc cctctaaata cgttaatttc ttgtatctgg ccagccacta
     7861 tgaaaagctc aaagggtctc ccgaagataa tgagcagaag cagctgttcg tggaacaaca
     7921 caaacactac cttgatgaga tcatcgagca aataagcgaa ttctccaaaa gagtgatcct
     7981 cgccgacgct aacctcgata aggtgctttc tgcttacaat aagcacaggg ataagcccat
     8041 cagggagcag gcagaaaaca ttatccactt gtttactctg accaacttgg gcgcgcctgc
     8101 agccttcaag tacttcgaca ccaccataga cagaaagcgg tacacctcta caaaggaggt
     8161 cctggacgcc acactgattc atcagtcaat tacggggctc tatgaaacaa gaatcgacct
     8221 ctctcagctc ggtggagaca gcagggctga ccccaagaag aagaggaagg tggctagcga
     8281 tgctaagtca ctgactgcct ggtcccggac actggtgacc ttcaaggatg tgtttgtgga
     8341 cttcaccagg gaggagtgga agctgctgga cactgctcag cagatcctgt acagaaatgt
     8401 gatgctggag aactataaga acctggtttc cttgggttat cagcttacta agccagatgt
     8461 gatcctccgg ttggagaagg gagaagagcc ctggctggtg gagagagaaa ttcaccaaga
     8521 gacccatcct gattcagaga ctgcatttga aatcaaatca tcagttccga aaaagaaacg
     8581 caaagttgct agcgagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc
     8641 cggccctggt accgtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     8701 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     8761 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     8821 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga
     8881 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     8941 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     9001 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     9061 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa
     9121 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt
     9181 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     9241 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga
     9301 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     9361 gtacaagacc ggttgataat agataacttc gtatagcata cattatacga agttatgaat
     9421 tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
     9481 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     9541 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     9601 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     9661 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     9721 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     9781 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     9841 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     9901 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     9961 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
    10021 cctccccgca tcgataccgt cgacctcgag acctagaaaa acatggagca atcacaagta
    10081 gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg
    10141 tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag
    10201 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
    10261 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga
    10321 actacacacc agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag
    10381 taccagttga gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac
    10441 accctgtgag cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg
    10501 acagccgcct agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc
    10561 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
    10621 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
    10681 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc
    10741 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
    10801 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
    10861 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
    10921 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
    10981 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
    11041 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
    11101 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
    11161 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
    11221 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
    11281 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
    11341 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
    11401 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
    11461 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
    11521 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
    11581 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
    11641 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
    11701 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
    11761 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
    11821 cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta
    11881 tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc
    11941 cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct
    12001 cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac
    12061 cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg
    12121 ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg
    12181 ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc
    12241 cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt
    12301 gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
    12361 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
    12421 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
    12481 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
    12541 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
    12601 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
    12661 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
    12721 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
    12781 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
    12841 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
    12901 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
    12961 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
    13021 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
    13081 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
    13141 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
    13201 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
    13261 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
    13321 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
    13381 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
    13441 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
    13501 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
    13561 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
    13621 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
    13681 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
    13741 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
    13801 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
    13861 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    13921 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    13981 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    14041 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    14101 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    14161 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    14221 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    14281 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    14341 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    14401 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    14461 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    14521 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    14581 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    14641 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    14701 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
    14761 gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag
    14821 ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa
    14881 atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt
    14941 aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgtt
//
