LOCUS       pLS408                  2749 bp    DNA     circular SYN 22-NOV-2024
DEFINITION  LMBP 9329.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 22, 2024 from SnapGene 8.0.0
            https://www.snapgene.com
COMMENT     Accession number: LMBP 9329
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium) 
            Last modified: 16-MAR-2017
FEATURES             Location/Qualifiers
     source          1..2749
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          1..1609
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     promoter        1..64
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             65..925
                     /codon_start=1
                     /label=AmpR
                     /label=ampicillin resistance
                     /label=ampicillin\resistance
                     /note="color: #993366"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    926..947
                     /label=AmpR TER
                     /note="color: #ffffff"
     rep_origin      948..1536
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     primer_bind     1537..1553
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     misc_feature    1554..1609
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
                     /note="color: #a6acb3"
     primer_bind     complement(1610..1626)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     promoter        1627..1970
                     /label=TEF pr
                     /note="Ashbya gossypii TEF promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             1971..2546
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=natMX6
                     /note="confers resistance to nourseothricin"
                     /note="color: #ff6600"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVISYSAWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFAGERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGERQALYMSMPCP"
     terminator      2552..2749
                     /label=TEF 3'-UTR
                     /note="Ashbya gossypii TEF terminator"
                     /note="color: #ffff99"
ORIGIN
        1 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
       61 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
      121 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgcagaag atcagttggg
      181 tgcgcgagtg ggttacatcg aactggacct caacagtggt aagatactag agagttttcg
      241 ccccgaagaa cgattcccaa tgatgtccac ttttaaggtt ctgctatgtg gcgcggtatt
      301 atcccgtatt gacgcggggc aagagcaact aggtcgccgc atacactatt ctcagaatga
      361 cttggttgag tattcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
      421 attatgtagt gctgccataa cgatgagtga taacacggcg gcgaacttac ttctgacaac
      481 gattggagga ccaaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
      541 ccttgatcgt tgggaaccag agctgaatga agccatacca aacgacgagc gtgacaccac
      601 gatgcctgtt gctatggcaa caaccttgcg gaaactatta actggcgaac tacttactct
      661 agcttcccgg caacagttaa tagactggat ggaggctgat aaagttgcag gaccacttct
      721 gcgctctgcg cttccggctg gctggtttat tgctgataaa tctggggccg gagagcgtgg
      781 gtcacgcggt atcatagcag cactagggcc agatggtaag ccctcccgta tcgtagttat
      841 ctacacgacg gggagccagg caactatgga tgaacgaaat agacagatcg ctgagatagg
      901 tgcgtcactg attaagcatt ggtagtagaa aagatcaaag gatcttcttg agatcctttt
      961 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     1021 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     1081 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     1141 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     1201 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     1261 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     1321 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     1381 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     1441 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     1501 ttgtgatgct cgtcaggggg gcggagccta tggaaacagg aaacagctat gacgcgcgcg
     1561 agctcggtac ccgggatcct ctagagtcga cctgcaggca tgccctagga ctggccgtcg
     1621 ttttacgaca tggaggccca gaataccctc cttgacagtc ttgacgtgcg cagctcaggg
     1681 gcatgatgtg actgtcgccc gtacatttag cccatacatc cccatgtata atcatttgca
     1741 tccatacatt ttgatggccg cacggcgcga agcaaaaatt acggctcctc gctgcagacc
     1801 tgcgagcagg gaaacgctcc cctcacagac gcgttgaatt gtccccacgc cgcgcccctg
     1861 tagagaaata taaaaggtta ggatttgcca ctgaggttct tctttcatat acttcctttt
     1921 aaaatcttgc taggatacag ttctcacatc acatccgaac ataaacaacc atgggaacca
     1981 ctcttgacga cacggcttac cgctaccgca ccagtgttcc gggggacgca gaggcaatcg
     2041 aggcactgga tggttccttc accaccgaca ccgtattccg cgtcaccgcc accggggacg
     2101 gcttcaccct gcgggaagtg ccagtggacc cgcccctgac caaagtgttc cccgacgacg
     2161 aatcggacga cgaatcggac gacggggagg acggcgaccc ggactcacgg acgttcgtag
     2221 cgtatgggga cgacggcgac ctggcgggct tcgtggtcat ctcgtactca gcgtggaacc
     2281 gacggctgac cgtcgaggac atcgaggtag ccccggaaca ccggggtcac ggggtcgggc
     2341 gagcgttgat ggggctggcg acggagttcg caggcgaacg gggggctggt catctctggc
     2401 tggaagtcac caacgtaaac gcacccgcga tccacgcgta ccgacgaatg gggttcaccc
     2461 tctgcggcct ggacaccgcc ctgtacgacg ggaccgcctc ggacggcgaa cggcaggcac
     2521 tctacatgag tatgccctgc ccctaatcaa tactgacaat aaaaagattc ttgttttcaa
     2581 gaacttgtca tttgtatagt ttttttatat tgtagttgtt ctattttaat caaatgttag
     2641 cgtgatttat attttttttc gcctcgacat catctgccca gatgcgaagt taagtgcgca
     2701 gaaagtaata tcatgcgtca atcgtatgtg aatgatggtc gctatactg
//
