LOCUS       pBK-EcWRS(H14)          2986 bp ds-DNA     circular     25-NOV-2024
DEFINITION  .
COMMENT     LOCUS       pBK-MJYRS-wt\(se        2881 bp    DNA     circular
            3-MAY-2007 SOURCE         ORGANISM   COMMENT     This file is
            created by Vector NTI             http://www.invitrogen.com/
            COMMENT     VNTDATE|426601173| COMMENT     VNTDBDATE|445450179|
            COMMENT     LSOWNER| COMMENT     VNTNAME|pBK-MJYRS-wt (sequence
            verified)| COMMENT     VNTAUTHORNAME|Michael Jahnz| COMMENT
            VNTAUTHORTEL|858-784-9336| COMMENT
            VNTAUTHOREML|jahnz@scripps.edu| COMMENT     VNTREPLTYPE|Plasmid
            COMMENT     VNTEXTCHREPL|Bacteria
FEATURES             Location/Qualifiers
     misc_feature    1..30
                     /label="CTTTTGCTGAGTTGAAGGATCCGCGGCCGC"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          4..27
                     /label="pBK-MCS-BamH-F"
                     /note="sequence: TTGCTGAGTTGAAGGATCCGCGGC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          6..30
                     /label="pBK-BamHI-F2"
                     /note="sequence: gctgagttgaaggatccGCGGCCGC"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          7..37
                     /label="pBK-BamHI-F"
                     /note="sequence: ctgagttgaaggatccGCGGCCGCtcgggtt"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(30..59)
                     /label="pBK-backbone-R"
                     /note="sequence: CTTATAAGCGGGACAGGCTGACAACCCGAG"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    37..66
                     /label="TGTCAGCCTGTCCCGCTTATAAGATCATAC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     promoter        59..65
                     /label="constitutive glutamine promotor"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     RBS             92..97
                     /label="rbs"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    94..153
                     /label="GAATCCCATATGATGACTAAGCCCATCGTTnnnnnnGGCGCACAGCCCTCAGGTGAATTG"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    94..153
                     /label="GAATCCCATATGATGACTAAGCCCATCGTTnnkAGTGGCGCACAGCCCTCAGGTGAATTG"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    94..153
                     /label="GAATCCCATATGATGACTAAGCCCATCGtttttNSTggcGCACAGCCCTCAGGTGAATTG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          100..131
                     /label="EcWRS-NdeI-F"
                     /note="sequence: attattacaTATGatgactaagcccatcgtttttagtgg"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    106..123
                     /label="aataataGCTAGCgccaccATGACTAAGCCCATCGTT"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    106..126
                     /label="AATAATAgcggccgcATGACTAAGCCCATCGTTTTT"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          106..133
                     /label="EcWRS-NdeI-F2"
                     /note="sequence: attattacatatgactaagcccatcgtttttagtggcg"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    106..1110
                     /label="Tryptophanyl-tRNA synthetase"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             106..1110
                     /label="Translation 106-1110"
                     /translation="MTKPIVFAGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQHAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFGELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLGPCGEDQKQHLELSRDIAQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVKKIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGEVADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP*"
     primer          109..133
                     /label="EcWRS-NdeI-10xHisN-F"
                     /note="sequence: attattacatatgcatcatcatcaccaccatcatcatcaccatactaagcccatcgtttttagtggcg"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    124..126
                     /label="Naphthalene Optional"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(181..202)
                     /label="TrpRS-R-Seq"
                     /note="sequence: cctgcatgtttacccactgacg"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(184..228)
                     /label="GATACAGTAAATGCAATGGTAGTCATCCTGCATGTTTACCCACTG"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    184..267
                     /label="CAGTGGGTAAACATGCAGGATGACTACCATTGCATTTACTGTATCnbtGACCAAnnkGCGATCACCGTGCGCCAGGATGCACAG"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    229..231
                     /label="(optional)"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    238..240
                     /label="Naphthalene"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(476..507)
                     /label="CGCTGCCATCAGCACCGGATAGTCAAACAGAC"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    476..555
                     /label="GTCTGTTTGACTATCCGGTGCTGATGGCAGCGnnknnkCTGCTGTATCAAACTAATCTGGTACCGGTGGGTGAAGACCAG"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    complement(498..534)
                     /label="CAGATTAGTTTGATACAGCAGGATGTCCGCTGCCATC"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    498..576
                     /label="GATGGCAGCGGACATCCTGCTGTATCAAACTAATCTGnnknnknnkGGTGAAGACCAGAAACAGCACCTCGAACTGAGC"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    498..576
                     /label="GATGGCAGCGGACATCCTGCTGTATCAAACTAATCTGnnnnnnnnnGGTGAAGACCAGAAACAGCACCTCGAACTGAGC"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    508..510
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    511..513
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          538..565
                     /label="EcWRS-145Fo"
                     /note="sequence: CCGGTGGGTGAAGACCAGAAACAGCACC"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          568..597
                     /label="EcWRS 155 Int-F"
                     /note="sequence: GAACTGAGCCGCGATATTGCCCAGCGTTTC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          568..603
                     /label="EcWRS 155 Int F 2"
                     /note="sequence: GAACTGAGCCGCGATATTGCCCAGCGTTTCAACGCG"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          568..612
                     /label="EcWRS 155 Int F 3"
                     /note="sequence: GAACTGAGCCGCGATATTGCCCAGCGTTTCAACGCGCTGTATGGC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(602..633)
                     /label="PFI_Mut-iR"
                     /note="sequence: CTCCGGCACCTTAAAGATCTCGCCATACAGCG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          643..666
                     /label="PFI-strandBias-F"
                     /note="sequence: CCGAAATCTGGCGCGCGCGTAATG"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    complement(713..750)
                     /label="CGATTTCGGATCTTCCAGCAGGCCGATAACGTTATTGC"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    713..790
                     /label="GCAATAACGTTATCGGCCTGCTGGAAGATCCGAAATCGnnkGTGAAGAAAATCAAACGTGCGGTCACTGACTCCGACG"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    751..753
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    complement(1084..1110)
                     /label="agcgtttgaaactgcagccatggtaccTTACGGCTTCGCCACAAAACCAATCGC"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(1084..1111)
                     /label="EcWRS-10xHisC-HindIII-R"
                     /note="sequence: attattaaagcttttaatggtgatgatgatggtggtgatgatgatgcggcttcgccacaaaaccaatcgc"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(1086..1110)
                     /label="EcWRS-HindIII-R"
                     /note="sequence: attattaaagcttttacggcttcgccacaaaaccaatc"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          complement(1152..1179)
                     /label="pBK MCS JIsqR"
                     /note="sequence: gagatcatgtaggcctgataagcgtagc"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          1158..1191
                     /label="pBK-backbone-F"
                     /note="sequence: CTTATCAGGCCTACATGATCTCTGCAATATATTG"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     terminator      1260..1284
                     /label="terminator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     rep_origin      1357..1957
                     /label="ColE1 Origin"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    2090..2905
                     /label="KAN"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          2942..2964
                     /label="pBK_SeqT-F"
                     /note="sequence: attacgctgacttgacgggacgg"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
ORIGIN
        1 cttttgctga gttgaaggat ccGCGGCCGC tcgggttgtc agcctgtccc gcttataaga
       61 tcatacgccg ttatacgttg tttacgcttt gaggaatccc aTATGatgac taagcccatc
      121 gtttttgctg gcgcacagcc ctcaggtgaa ttgaccattg gtaactacat gggtgcgctg
      181 cgtcagtggg taaacatgca ggatgactac cattgcattt actgtatcgt tgaccaacac
      241 gcgatcaccg tgcgccagga tgcacagaag ctgcgtaaag cgacgctgga tacgctggcc
      301 ttgtatctgg cttgtggtat cgatcctgag aaaagcacca tttttgttca gtcccacgtg
      361 ccggaacatg cacagttagg ctgggcactg aactgctata cctacttcgg cgaactgagt
      421 cgcatgacgc agtttaaaga taaatctgcg cgttatgccg agaacatcaa cgctggtctg
      481 tttgactatc cggtgctgat ggcagcggac atcctgctgt atcaaactaa tctgggtcct
      541 tgtggtgaag accagaaaca gcacctcgaa ctgagccgcg atattgccca gcgtttcaac
      601 gcgctgtatg gcgagatctt taaggtgccg gagccgttta ttccgaaatc tggcgcgcgc
      661 gtaatgtcgc tgctggagcc gaccaagaag atgtccaagt ctgacgataa tcgcaataac
      721 gttatcggcc tgctggaaga tccgaaatcg gtagtgaaga aaatcaaacg tgcggtcact
      781 gactccgacg agccgccggt agttcgctac gatgtgcaga acaaagcggg cgtttccaac
      841 ctgttggata tcctttcagc ggtaacgggc cagagcatcc cagaactgga aaaacagttc
      901 gaaggcaaga tgtatggtca tctgaaaggt gaagtggctg atgccgtttc cggtatgctg
      961 actgaattgc aggaacgcta tcaccgtttc cgcaacgatg aagccttcct gcaacaggtg
     1021 atgaaagatg gcgcggaaaa agccagcgcg cacgcttccc gtacgctaaa agcggtgtac
     1081 gaagcgattg gttttgtggc gaagccgtaa GGTACCATGG CTGCAgtttc aaacgctaaa
     1141 ttgcctgatg cgctacgctt atcaggccta catgatctct gcaatatatt gagtttgcgt
     1201 gcttttgtag gccggataag gcgttcacgc cgcatccggc aagaaacagc aaacaatcca
     1261 aaacgccgcg ttcagcggcg ttttttctgc ttttcttcgc gaattaattc cgcttcgcac
     1321 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     1381 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     1441 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     1501 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     1561 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     1621 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     1681 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     1741 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     1801 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     1861 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     1921 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     1981 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     2041 atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat
     2101 tcaacgggaa acgtcttgct cgaggccgcg attaaattcc aacatggatg ctgatttata
     2161 tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgattgta
     2221 tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga
     2281 tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat
     2341 caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga tccccgggaa
     2401 aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct
     2461 ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga
     2521 tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag
     2581 tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa
     2641 gcttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct
     2701 tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga
     2761 ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca
     2821 gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca
     2881 tttgatgctc gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag
     2941 cattacgctg acttgacggg acggcggctt tgttgaataa atcgaa
//