LOCUS       pBK_MCS_EcWRS_H14-5HTP_ 4618 bp ds-DNA     circular     14-NOV-2024
DEFINITION  .
COMMENT     LOCUS       pBK-MJYRS-wt\(se        2881 bp    DNA     circular
            3-MAY-2007 SOURCE         ORGANISM   COMMENT     This file is
            created by Vector NTI             http://www.invitrogen.com/
            COMMENT     VNTDATE|426601173| COMMENT     VNTDBDATE|445450179|
            COMMENT     LSOWNER| COMMENT     VNTNAME|pBK-MJYRS-wt (sequence
            verified)| COMMENT     VNTAUTHORNAME|Michael Jahnz| COMMENT
            VNTAUTHORTEL|858-784-9336| COMMENT
            VNTAUTHOREML|jahnz@scripps.edu| COMMENT     VNTREPLTYPE|Plasmid
            COMMENT     VNTEXTCHREPL|Bacteria
FEATURES             Location/Qualifiers
     terminator      119..143
                     /label="terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     rep_origin      216..816
                     /label="ColE1 Origin"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    949..1764
                     /label="KAN"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     promoter        1975..1981
                     /label="constitutive glutamine promoter"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     RBS             2008..2013
                     /label="rbs"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    2019..3293
                     /label="EcYRS-OMeY"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             2019..3293
                     /label="Translation 2019-3293"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALVCGFDPTADSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVDKIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQRLNREGQGISFTEFSYNLLQGYSMACANKQYGVVLQIGGSDQWGNITSGIDLTRRLHQNQVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTARADVYRFLKFFTFMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSALSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQSDPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK*"
     misc_feature    2127..2129
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    complement(2562..2564)
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    2811..2813
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     terminator      3439..3463
                     /label="terminator"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     promoter        3536..3542
                     /label="constitutive glutamine promotor"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    3539..3568
                     /label="GlnRS promoter"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     RBS             3569..3574
                     /label="rbs"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    3583..4587
                     /label="Tryptophanyl-tRNA synthetase"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3601..3603
                     /label="NBT-lib1"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    4592..4593
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
ORIGIN
        1 tgcctgatgc gctacgctta tcaggcctac atgatctctg caatatattg agtttgcgtg
       61 cttttgtagg ccggataagg cgttcacgcc gcatccggca agaaacagca aacaatccaa
      121 aacgccgcgt tcagcggcgt tttttctgct tttcttcgcg aattaattcc gcttcgcaca
      181 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
      241 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
      301 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
      361 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
      421 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
      481 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
      541 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
      601 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
      661 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
      721 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
      781 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
      841 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
      901 tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt
      961 caacgggaaa cgtcttgctc gaggccgcga ttaaattcca acatggatgc tgatttatat
     1021 gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgattgtat
     1081 gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat
     1141 gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc
     1201 aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat ccccgggaaa
     1261 acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg
     1321 gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat
     1381 cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt
     1441 gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataag
     1501 cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt
     1561 atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac
     1621 cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag
     1681 aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat
     1741 ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc
     1801 attacgctga cttgacggga cggcggcttt gttgaataaa tcgaactttt gctgagttga
     1861 aggatccGCG GCCGCGTGCG AAGCGGAATT AATTCGCGAA GAAAAGCAGA AAAAACGCCG
     1921 CTGAACGCGG CGACCCGAGC GGCCGCtcgg gttgtcagcc tgtcccgctt ataagatcat
     1981 acgccgttat acgttgttta cgctttgagg aatcccatat ggcaagcagt aacttgatta
     2041 aacaattgca agagcggggg ctggtagccc aggtgacgga cgaggaagcg ttagcagagc
     2101 gactggcgca aggcccgatc gcgctcGTGt gcggcttcga tcctaccgct gacagcttgc
     2161 atttggggca tcttgttcca ttgttatgcc tgaaacgctt ccagcaggcg ggccacaagc
     2221 cggttgcgct ggtaggcggc gcgacgggtc tgattggcga cccgagcttc aaagctgccg
     2281 agcgtaagct gaacaccgaa gaaactgttc aggagtgggt ggacaaaatc cgtaagcagg
     2341 ttgccccgtt cctcgatttc gactgtggag aaaactctgc tatcgcggcg aacaactatg
     2401 actggttcgg caatatgaat gtgctgacct tcctgcgcga tattggcaaa cacttctccg
     2461 ttaaccagat gatcaacaaa gaagcggtta agcagcgtct caaccgtgaa ggtcagggga
     2521 tttcgttcac tgagttttcc tacaacctgt tgcagggtta tAgtatggcc tgtgcgaaca
     2581 aacagtacgg tgtggtgctg caaattggtg gttctgacca Gtggggtaac atcacttctg
     2641 gtatcgacct gacccgtcgt ctgcatcaga atcaggtgtt tggcctgacc gttccgctga
     2701 tcactaaagc agatggcacc aaatttggta aaactgaagg cggcgcagtc tggttagaTc
     2761 cgaagaaaac cagcccgtac aaattctacc agttctggat caacactgcg CGtgccgacg
     2821 tttaccgctt cctgaagttc ttcaccttta tgagcattga agagatcaac gccctggaag
     2881 aagaagataa aaacagcggt aaagcaccgc gcgcccagta tgtactggcg gagcaggtga
     2941 ctcgtctggt tcacggtgaa gaaggtttac aggcggcaaa acgtattacc gaatgcctgt
     3001 tcagcggttc tttgagtgcg ctgagtgaag cggacttcga acagctggcg caggacggcg
     3061 taccgatggt tgagatggaa aagggcgcag acctgatgca ggcactggtc gattctgaac
     3121 tgcaaccttc ccgtggtcag gcacgtaaaa ctatcgcctc caatgccatc accattaacg
     3181 gtgaaaaaca gtccgatcct gaatacttct ttaaagaaga agatcgtctg tttggtcgtt
     3241 ttaccttact gcgtcgcggt aaaaagaatt actgtctgat ttgctggaaa taaccatggc
     3301 tgcagtttca aacgctaaat tgcctgatgc gctacgctta tcaggcctac atgatctctg
     3361 caatatattg agtttgcgtg cttttgtagg ccggataagg cgttcacgcc gcatccggca
     3421 agaaacagca aacaatccaa aacgccgcgt tcagcggcgt tttTTCTGCT TTTCTTCGCG
     3481 AATTAATTCC GCTTCGCACG CGGCCGCtcg ggttgtcagc ctgtcccgct tataagatca
     3541 tacgccgtta tacgttgttt acgctttgag gaatcccaTA TGatgactaa gcccatcgtt
     3601 tttgctggcg cacagccctc aggtgaattg accattggta actacatggg tgcgctgcgt
     3661 cagtgggtaa acatgcagga tgactaccat tgcatttact gtatcgttga ccaacacgcg
     3721 atcaccgtgc gccaggatgc acagaagctg cgtaaagcga cgctggatac gctggccttg
     3781 tatctggctt gtggtatcga tcctgagaaa agcaccattt ttgttcagtc ccacgtgccg
     3841 gaacatgcac agttaggctg ggcactgaac tgctatacct acttcggcga actgagtcgc
     3901 atgacgcagt ttaaagataa atctgcgcgt tatgccgaga acatcaacgc tggtctgttt
     3961 gactatccgg tgctgatggc agcggacatc ctgctgtatc aaactaatct gggtccttgt
     4021 ggtgaagacc agaaacagca cctcgaactg agccgcgata ttgcccagcg tttcaacgcg
     4081 ctgtatggcg agatctttaa ggtgccggag ccgtttattc cgaaatctgg cgcgcgcgta
     4141 atgtcgctgc tggagccgac caagaagatg tccaagtctg acgataatcg caataacgtt
     4201 atcggcctgc tggaagatcc gaaatcggta gtgaagaaaa tcaaacgtgc ggtcactgac
     4261 tccgacgagc cgccggtagt tcgctacgat gtgcagaaca aagcgggcgt ttccaacctg
     4321 ttggatatcc tttcagcggt aacgggccag agcatcccag aactggaaaa acagttcgaa
     4381 ggcaagatgt atggtcatct gaaaggtgaa gtggctgatg ccgtttccgg tatgctgact
     4441 gaattgcagg aacgctatca ccgtttccgc aacgatgaag ccttcctgca acaggtgatg
     4501 aaagatggcg cggaaaaagc cagcgcgcac gcttcccgta cgctaaaagc ggtgtacgaa
     4561 gcgattggtt ttgtggcgaa gccgtaaggt accatggctg cagtttcaaa cgctaaat
//