LOCUS       pdl655                  9328 bp    DNA        circular     22-NOV-2024
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    243..268
                     /locus_tag="T-DNA border (R)"
                     /label="T-DNA border (R)"
                     /ApEinfo_label="T-DNA border (R)"
                     /ApEinfo_fwdcolor="#b4abac"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    complement(1310..2310)
                     /locus_tag="STA region from pVS1"
                     /label="STA region from pVS1"
                     /ApEinfo_label="STA region from pVS1"
                     /ApEinfo_fwdcolor="#d59687"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     rep_origin      complement(2903..3903)
                     /locus_tag="pVS1 ori"
                     /label="pVS1 ori"
                     /ApEinfo_label="pVS1 ori"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    complement(4313..4573)
                     /locus_tag="bom site from pBR322"
                     /label="bom site from pBR322"
                     /ApEinfo_label="bom site from pBR322"
                     /ApEinfo_fwdcolor="#faac61"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     rep_origin      complement(4713..4993)
                     /locus_tag="pBR322 ori"
                     /label="pBR322 ori"
                     /ApEinfo_label="pBR322 ori"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     gene            complement(5284..6078)
                     /locus_tag="KanR"
                     /label="KanR"
                     /ApEinfo_label="KanR"
                     /ApEinfo_fwdcolor="#faac61"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    6503..6528
                     /locus_tag="T-DNA border (L)"
                     /label="T-DNA border (L)"
                     /ApEinfo_label="T-DNA border (L)"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     terminator      complement(6595..6798)
                     /locus_tag="35S terminator"
                     /label="35S terminator"
                     /ApEinfo_label="35S terminator"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     cds             complement(6836..7633)
                     /locus_tag="neomycin phosphotransferase (kanR)"
                     /label="neomycin phosphotransferase (kanR)"
                     /ApEinfo_label="neomycin phosphotransferase (kanR)"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     promoter        complement(7664..8443)
                     /locus_tag="2x35S promoter"
                     /label="2x35S promoter"
                     /ApEinfo_label="2x35S promoter"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     promoter        8742..9186
                     /note=""label: AtU6 promoter note: /created_by=User note:"
                     /modified_by="User""
                     /locus_tag="AtU6 promoter"
                     /label="AtU6 promoter"
                     /ApEinfo_label="AtU6 promoter"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    9207..9292
                     /locus_tag=">esgRNA"
                     /label=">esgRNA"
                     /ApEinfo_label=">esgRNA"
                     /ApEinfo_fwdcolor="#b4abac"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     source          9207..9292
                     /note="label: source:unspecified"
                     /locus_tag="source:unspecified"
                     /label="source:unspecified"
                     /ApEinfo_label="source:unspecified"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
                     /ApEinfo_hidden
ORIGIN
        1 tggcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
       61 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
      121 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ctagagcagc ttgagcttgg
      181 atcagattgt cgtttcccgc cttcagttta aactatcagt gtttgacagg atatattggc
      241 gggtaaacct aagagaaaag agcgtttatt agaataatcg gatatttaaa agggcgtgaa
      301 aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc cctcgggatc
      361 aaagtacttt gatccaaccc ctccgctgct atagtgcagt cggcttctga cgttcagtgc
      421 agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag gctgccgccc
      481 tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag aatacttgcg
      541 actagaaccg gagacattac gccatgaaca agagcgccgc cgctggcctg ctgggctatg
      601 cccgcgtcag caccgacgac caggacttga ccaaccaacg ggccgaactg cacgcggccg
      661 gctgcaccaa gctgttttcc gagaagatca ccggcaccag gcgcgaccgc ccggagctgg
      721 ccaggatgct tgaccaccta cgccctggcg acgttgtgac agtgaccagg ctagaccgcc
      781 tggcccgcag cacccgcgac ctactggaca ttgccgagcg catccaggag gccggcgcgg
      841 gcctgcgtag cctggcagag ccgtgggccg acaccaccac gccggccggc cgcatggtgt
      901 tgaccgtgtt cgccggcatt gccgagttcg agcgttccct aatcatcgac cgcacccgga
      961 gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg cccccgccct accctcaccc
     1021 cggcacagat cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc gtgaaagagg
     1081 cggctgcact gcttggcgtg catcgctcga ccctgtaccg cgcacttgag cgcagcgagg
     1141 aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg
     1201 ccgacgccct ggcggccgcc gagaatgaac gccaagagga acaagcatga aaccgcacca
     1261 ggacggccag gacgaaccgt ttttcattac cgaagagatc gaggcggaga tgatcgcggc
     1321 cgggtacgtg ttcgagccgc ccgcgcacgg ctcaaccgtg cggctgcatg aaatcctggc
     1381 cggtttgtct gatgccaagc tggcggcctg gccggccagc ttggccgctg aagaaaccga
     1441 gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc
     1501 tgcgtatatg atgcgatgag taaataaaca aatacgcaag gggaacgcat gaaggttatc
     1561 gctgtactta accagaaagg cgggtcaggc aagacgacca tcgcaaccca tctagcccgc
     1621 gccctgcaac tcgccggggc cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc
     1681 cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg
     1741 acgattgacc gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat cgacggagcg
     1801 ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg
     1861 gtgcagccaa gcccttacga catatgggcc accgccgacc tggtggagct ggttaagcag
     1921 cgcattgagg tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa
     1981 ggcacgcgca tcggcggtga ggttgccgag gcgctggccg ggtacgagct gcccattctt
     2041 gagtcccgta tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt
     2101 cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt
     2161 aaatcaaaac tcatttgagt taatgaggta aagagaaaat gagcaaaagc acaaacacgc
     2221 taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc agcctggcag
     2281 acacgccagc catgaagcgg gtcaactttc agttgccggc ggaggatcac accaagctga
     2341 agatgtacgc ggtacgccaa ggcaagacca ttaccgagct gctatctgaa tacatcgcgc
     2401 agctaccaga gtaaatgagc aaatgaataa atgagtagat gaattttagc ggctaaagga
     2461 ggcggcatgg aaaatcaaga acaaccaggc accgacgccg tggaatgccc catgtgtgga
     2521 ggaacgggcg gttggccagg cgtaagcggc tgggttgtct gccggccctg caatggcact
     2581 ggaaccccca agcccgagga atcggcgtga cggtcgcaaa ccatccggcc cggtacaaat
     2641 cggcgcggcg ctgggtgatg acctggtgga gaagttgaag gccgcgcagg ccgcccagcg
     2701 gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat
     2761 ccgcaaagaa tcccggcaac cgccggcagc cggtgcgccg tcgattagga agccgcccaa
     2821 gggcgacgag caaccagatt ttttcgttcc gatgctctat gacgtgggca cccgcgatag
     2881 tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac gagctggcga
     2941 ggtgatccgc tacgagcttc cagacgggca cgtagaggtt tccgcagggc cggccggcat
     3001 ggccagtgtg tgggattacg acctggtact gatggcggtt tcccatctaa ccgaatccat
     3061 gaaccgatac cgggaaggga agggagacaa gcccggccgc gtgttccgtc cacacgttgc
     3121 ggacgtactc aagttctgcc ggcgagccga tggcggaaag cagaaagacg acctggtaga
     3181 aacctgcatt cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa
     3241 cggccgcctg gtgacggtat ccgagggtga agccttgatt agccgctaca agatcgtaaa
     3301 gagcgaaacc gggcggccgg agtacatcga gatcgagcta gctgattgga tgtaccgcga
     3361 gatcacagaa ggcaagaacc cggacgtgct gacggttcac cccgattact ttttgatcga
     3421 tcccggcatc ggccgttttc tctaccgcct ggcacgccgc gccgcaggca aggcagaagc
     3481 cagatggttg ttcaagacga tctacgaacg cagtggcagc gccggagagt tcaagaagtt
     3541 ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg atttgaagga
     3601 ggaggcgggg caggctggcc cgatcctagt catgcgctac cgcaacctga tcgagggcga
     3661 agcatccgcc ggttcctaat gtacggagca gatgctaggg caaattgccc tagcagggga
     3721 aaaaggtcga aaaggcctct ttcctgtgga tagcacgtac attgggaacc caaagccgta
     3781 cattgggaac cggaacccgt acattgggaa cccaaagccg tacattggga accggtcaca
     3841 catgtaagtg actgatataa aagagaaaaa aggcgatttt tccgcctaaa actctttaaa
     3901 acttattaaa actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc
     3961 ccaagagctg caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc
     4021 gcgtcggcct atcgcggccg ctggccgctc aaaaatggct ggcctacggc caggcaatct
     4081 accagggcgc ggacaagccg cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac
     4141 cctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaaa
     4201 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     4261 cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt
     4321 atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
     4381 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggccctctt ccgcttcctc
     4441 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     4501 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     4561 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     4621 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     4681 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     4741 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     4801 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     4861 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     4921 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     4981 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     5041 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     5101 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     5161 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     5221 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgcattctag
     5281 gtactaaaac aattcatcca gtaaaatata atattttatt ttctcccaat caggcttgat
     5341 ccccagtaag tcaaaaaata gctcgacata ctgttcttcc ccgatatcct ccctgatcga
     5401 ccggacgcag aaggcaatgt cataccactt gtccgccctg ccgcttctcc caagatcaat
     5461 aaagccactt actttgccat ctttcacaaa gatgttgctg tctcccaggt cgccgtggga
     5521 aaagacaagt tcctcttcgg gcttttccgt ctttaaaaaa tcatacagct cgcgcggatc
     5581 tttaaatgga gtgtcttctt cccagttttc gcaatccaca tcggccagat cgttattcag
     5641 taagtaatcc aattcggcta agcggctgtc taagctattc gtatagggac aatccgatat
     5701 gtcgatggag tgaaagagcc tgatgcactc cgcatacagc tcgataatct tttcagggct
     5761 ttgttcatct tcatactctt ccgagcaaag gacgccatcg gcctcactca tgagcagatt
     5821 gctccagcca tcatgccgtt caaagtgcag gacctttgga acaggcagct ttccttccag
     5881 ccatagcatc atgtcctttt cccgttccac atcataggtg gtccctttat accggctgtc
     5941 cgtcattttt aaatataggt tttcattttc tcccaccagc ttatatacct tagcaggaga
     6001 cattccttcc gtatctttta cgcagcggta tttttcgatc agttttttca attccggtga
     6061 tattctcatt ttagccattt attatttcct tcctcttttc tacagtattt aaagataccc
     6121 caagaagcta attataacaa gacgaactcc aattcactgt tccttgcatt ctaaaacctt
     6181 aaataccaga aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa catagtatcg
     6241 acggagccga ttttgaaacc gcggtgatca caggcagcaa cgctctgtca tcgttacaat
     6301 caacatgcta ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc
     6361 ttccgaatag catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga
     6421 cgccgtcccg gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt
     6481 cggggagctg ttggctggct ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta
     6541 gacaacttaa taacacattg cggacgtttt taatgtactg aattaacgcc gaattaattc
     6601 gggggatctg gattttagta ctggattttg gttttaggaa ttagaaattt tattgataga
     6661 agtattttac aaatacaaat acatactaag ggtttcttat atgctcaaca catgagcgaa
     6721 accctatagg aaccctaatt cccttatctg ggaactactc acacattatt atggagaaac
     6781 tcgagcttgt cgatcgactc tagctagagg atcgatccga accccagagt cccgctcaga
     6841 agaactcgtc aagaaggcga tagaaggcga tgcgctgcga atcgggagcg gcgataccgt
     6901 aaagcacgag gaagcggtca gcccattcgc cgccaagttc ttcagcaata tcacgggtag
     6961 ccaacgctat gtcctgatag cggtccgcca cacccagccg gccacagtcg atgaatccag
     7021 aaaagcggcc attttccacc atgatattcg gcaagcaggc atcgccatgt gtcacgacga
     7081 gatcctcgcc gtcgggcatg cgcgccttga gcctggcgaa cagttcggct ggcgcgagcc
     7141 cctgatgttc ttcgtccaga tcatcctgat cgacaagacc ggcttccatc cgagtacgtg
     7201 ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca ggtagccgga tcaagcgtat
     7261 gcagccgccg cattgcatca gccatgatgg atactttctc ggcaggagca aggtgagatg
     7321 acaggagatc ctgccccggc acttcgccca atagcagcca gtcccttccc gcttcagtga
     7381 caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc cagccacgat agccgcgctg
     7441 cctcgtcctg gagttcattc agggcaccgg acaggtcggt cttgacaaaa agaaccgggc
     7501 gcccctgcgc tgacagccga aacacggcgg catcagagca gccgattgtc tgttgtgccc
     7561 agtcatagcc gaatagcctc tccacccaag cggccggaga acctgcgtgc aatccatctt
     7621 gttcaatccc catggtcgat cgacagatct gcgaaagctc gagagagata gatttgtaga
     7681 gagagactgg tgatttcagc gtgtcctctc caaatgaaat gaacttcctt atatagagga
     7741 agggtcttgc gaaggatagt gggattgtgc gtcatccctt acgtcagtgg agatatcaca
     7801 tcaatccact tgctttgaag acgtggttgg aacgtcttct ttttccacga tgctcctcgt
     7861 gggtgggggt ccatctttgg gaccactgtc ggcagaggca tcttgaacga tagcctttcc
     7921 tttatcgcaa tgatggcatt tgtaggtgcc accttccttt tctactgtcc ttttgatgaa
     7981 gtgacagata gctgggcaat ggaatccgag gaggtttccc gatattaccc tttgttgaaa
     8041 agtctcaata gccctttggt cttctgagac tgtatctttg atattcttgg agtagacgag
     8101 agtgtcgtgc tccaccatgt tcacatcaat ccacttgctt tgaagacgtg gttggaacgt
     8161 cttctttttc cacgatgctc ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag
     8221 aggcatcttg aacgatagcc tttcctttat cgcaatgatg gcatttgtag gtgccacctt
     8281 ccttttctac tgtccttttg atgaagtgac agatagctgg gcaatggaat ccgaggaggt
     8341 ttcccgatat taccctttgt tgaaaagtct caatagccct ttggtcttct gagactgtat
     8401 ctttgatatt cttggagtag acgagagtgt cgtgctccac catgttggca agctgctcta
     8461 gccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     8521 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     8581 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     8641 gagcggataa caatttcaca caggaaacag ctatgacatg attacgaatt cgagctcggt
     8701 acccggggat cggcgcgccG ATATCgagct cGGACccatg gcttcgttga acaacggaaa
     8761 ctcgacttgc cttccgcaca atacatcatt tcttcttagc tttttttctt cttcttcgtt
     8821 catacagttt ttttttgttt atcagcttac attttcttga accgtagctt tcgttttctt
     8881 ctttttaact ttccattcgg agtttttgta tcttgtttca tagtttgtcc caggattaga
     8941 atgattaggc atcgaacctt caagaatttg attgaataaa acatcttcat tcttaagata
     9001 tgaagataat cttcaaaagg cccctgggaa tctgaaagaa gagaagcagg cccatttata
     9061 tgggaaagaa caatagtatt tcttatatag gcccatttaa gttgaaaaca atcttcaaaa
     9121 gtcccacatc gcttagataa gaaaacgaag ctgagtttat atacagctag agtcgaagta
     9181 gtgattGGAC TCTTGCCAGC AATGCTgttt aagagctatg ctggaaacag catagcaagt
     9241 ttaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gctttttttc
     9301 ccgggGcgcg ccGATATCga gctcAGTG   
//
