LOCUS       CA-0244_CMVTO_ERret-H2M 8745 bp ds-DNA     circular     26-NOV-2024
DEFINITION  .
COMMENT     Concatenation of 4 sequences.
FEATURES             Location/Qualifiers
     CDS             complement(570..1430)
                     /label="Amp (bla)"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     CDS             complement(573..1232)
                     /label="AmpR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     promoter        complement(1431..1529)
                     /label="bla promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          1525..1541
                     /label="AmpR_seq_F"
                     /note="sequence: ggggttccgcgcacatt"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     promoter        1793..2420
                     /label="CMV"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        1872..2175
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     promoter        2176..2379
                     /label="CMV promoter"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          2330..2350
                     /label="CMV-Forward Genewiz"
                     /note="sequence: CGCAAATGGGCGGTAGGCGTG"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     TATA_signal     2365..2371
                     /label="TATA box"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2381..2399
                     /label="TetO"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2381..2420
                     /label="TetR binding sites"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2402..2420
                     /label="TetO"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          2481..2501
                     /label="CMVF1"
                     /note="sequence: tgacctccatagaagacaccg"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2527..2543
                     /label="INF"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          2527..2548
                     /label="CA-0084.F"
                     /note="sequence: gcgtttaaacttaagcttcgcc"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          2527..2572
                     /label="CA-0184.F"
                     /note="sequence: gcgtttaaacttaagcttcgccaccATGGACGGTTGGAGAAGGATG"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    2544..2551
                     /label="Kozak"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2552..2572
                     /label="PCR FWD"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    2552..2620
                     /label="Renin Signal Sequence (human)"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             2552..2620
                     /label="Translation 2552-2620"
                     /translation="MDGWRRMPRWGLLLLLWGSCTFG"
     misc_feature    2609..2620
                     /label="INF"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             2621..2623
                     /label="Translation 2621-2623"
                     /translation="A"
     CDS             2624..2626
                     /label="Translation 2624-2626"
                     /translation="S"
     CDS             2627..3646
                     /label="H2M-Renin"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             2627..3646
                     /label="Translation 2627-3646"
                     /translation="LTLGNTTSSVILTNYMDTQYYGEIGIGTPPQTFKVVFDTGSSNVWVPSSKCSRLYTACVYHKLFDASDSSSYKHNGTELTIHYSTGTVSGFLSQDIITVGGITVTQMFGEVTEMPALPFMLAEFDGVVGMGFIEQAVGRVTPIFDNIISQGVLKEDVFSFYYNRDSENSQSLGGQIVLGGSDPQHYEGNFHYINLIKTGVWQIQMKGVSVGSSTLLCEDGCLAVVDTGASYISGSTSSIEKLMEALGAKKRLFDYVVKCNEGPTLPDISFHLGGKEYTLTSADYVFQESYSSKKLCTLAIHAMDIPPPTGPTWALGATFIRKFYTEFDRRNNRIGFALAR"
     misc_feature    2639..2641
                     /label="Glycosylation Site #1"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          2708..2729
                     /label="Renin1"
                     /note="sequence: ACACCCCCTCAGACTTTTAAGG"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2777..2800
                     /label="Disulfide #1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2846..2848
                     /label="Histidine #1"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    2849..2851
                     /label="Glycosylation Site #2"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    2867..2869
                     /label="L->I"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2870..2872
                     /label="R->H"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          3002..3018
                     /label="Renin-2-seq"
                     /note="sequence: GGGGTCGTTGGCATGGG"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    3035..3037
                     /label="I->V"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          3116..3142
                     /label="Renseq_F"
                     /note="sequence: AGAGATTCTGAAAACTCTCAGAGTTTG"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    3179..3181
                     /label="Histidine #2"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    3197..3199
                     /label="Histidine #3"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    3275..3289
                     /label="Disulfide #2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    3296..3298
                     /label="L->V"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    3401..3514
                     /label="Disulfide #3"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    3437..3439
                     /label="Histidine #4"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    3527..3529
                     /label="Histidine #5"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    3629..3646
                     /label="PCR REV"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(3629..3664)
                     /label="CA-0076.R"
                     /note="sequence: ccctcctgtcccggcgccCCTGGCAAGTGCAAAGCC"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             3647..3769
                     /label="45-ECL"
                     /ApEinfo_revcolor="#339966"
                     /ApEinfo_fwdcolor="#339966"
     CDS             3647..3769
                     /label="Translation 3647-3769"
                     /translation="GAGTGGSGGGTGGSGGDYKDDDDNGGSGGSGGSGGSGGGTG"
     misc_feature    3650..3664
                     /label="INF REV"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             3770..3775
                     /label="Translation 3770-3775"
                     /translation="GS"
     misc_feature    3776..3841
                     /label="CD4 TM"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             3776..3841
                     /label="Translation 3776-3841"
                     /translation="MALIVLGGVAGLLLFIGLGIFF"
     CDS             3842..3886
                     /label="Translation 3842-3886"
                     /translation="GGGSLENLYFQSGSG"
     CDS             3857..3877
                     /label="TEVsite"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     CDS             3887..3892
                     /label="Translation 3887-3892"
                     /translation="GT"
     misc_feature    3893..4000
                     /label="C-Terminal Tail of Kir6.2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             3893..4000
                     /label="Translation 3893-4000"
                     /translation="LLEALTLASARGPLRKRSVPMAKAKPKFSISPDSLS"
     misc_feature    3935..3943
                     /label="RXR Retention Motif"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    3981..4000
                     /label="PCR REV"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(3981..4033)
                     /label="CA-0162.R"
                     /note="sequence: ATCGCCACAGGTAAGCAAACTCCCCCGACCTTCGCTCAAGCTGTCAGGTGAGA"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             4001..4054
                     /label="T2A"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             4001..4054
                     /label="Translation 4001-4054"
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    4019..4033
                     /label="INF"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          4019..4070
                     /label="CA-0163.F"
                     /note="sequence: CTTACCTGTGGCGATGTCGAGGAGAATCCAGGACCCATGCACTCCTCCGCGC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    4055..4070
                     /label="PCR FWD"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    4055..4108
                     /label="IL10 signal peptide"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             4055..4108
                     /label="Translation 4055-4108"
                     /translation="MHSSALLCCLVLLTGVRA"
     misc_feature    4109..4606
                     /label="hIL10 monomer (R5A11M)"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             4109..4606
                     /label="Translation 4109-4606"
                     /translation="SPGQGTQSENSCTHFPGILPNMLRDLRDAFSRVKTFFQMKDQLDNLLLKESLLEDFKGYLGCQALSEMIQFYLEEVMPQAENQDPDIKAHVISLGENLNTLRLRLRRCHRLLPCENGGGSGGKSKAVEQVKNAFNKLQEKGIYKAMSEFDIFINYIEAYMTMKIRN"
     misc_feature    4457..4474
                     /label="Extended linker"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    4580..4606
                     /label="PCR REV1"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(4580..4636)
                     /label="CA-0105.R1"
                     /note="sequence: GAGCAGGTGGAAGGGATGGATGTAGGCGCCGTTACGAATTTTCATGGTCATGTAGGC"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     CDS             4607..4612
                     /label="Translation 4607-4612"
                     /translation="GA"
     misc_feature    4607..4612
                     /label="for cloning"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    4613..4651
                     /label="Renin cleavage site (mouse)"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             4613..4651
                     /label="Translation 4613-4651"
                     /translation="YIHPFHLLYHNKS"
     misc_feature    4622..4636
                     /label="INF"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          4622..4667
                     /label="CA-0105.F2"
                     /note="sequence: CCCTTCCACCTGCTCTACCACAACAAGAGCGGCGGTGGGGGATCTG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             4652..4666
                     /label="Translation 4652-4666"
                     /translation="GGGGS"
     misc_feature    4652..4667
                     /label="PCR"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             4667..4681
                     /label="Translation 4667-4681"
                     /translation="GGGGS"
     CDS             4682..4696
                     /label="Translation 4682-4696"
                     /translation="GGGGS"
     misc_feature    4697..5338
                     /label="hIL10Ra (Extracellular)"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             4697..5338
                     /label="Translation 4697-5338"
                     /translation="HGTELPSPPSVWFEAEFFHHILHWTPIPNQSESTCYEVALLRYGIESWNSISNCSQTLSYDLTAVTLDLYHSNGYRARVRAVDGSRHSNWTVTNTRFSVDEVTLTVGSVNLEIHNGFILGKIQLPRPKMAPANDTYESIFSHFREYEIAIRKVPGNFTFTHKKVKHENFSLLTSGEVGEFCVQVKPSVASRSNKGMWSKEECISLTRQYFTVTN"
     misc_feature    5316..5338
                     /label="PCR REV"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(5316..5383)
                     /label="CA-0163.R"
                     /note="sequence: ACTTCCCCGTCCCTCCAGGTCCTCTTCGCTGATGAGCTTCTGCTCATTGGTGACCGTAAAATACTGCC"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    5339..5368
                     /label="Myc-tag"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             5339..5368
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="EQKLISEEDL"
     misc_feature    5369..5383
                     /label="INF"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             5369..5422
                     /label="T2A (modified)"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             5369..5422
                     /label="Translation 5369-5422"
                     /translation="EGRGSLLTCGDVEENPGP"
     primer          5369..5439
                     /label="CA-0158.F"
                     /note="sequence: GAGGGACGGGGAAGTTTGCTTACCTGTGGCGATGTCGAGGAGAATCCAGGTCCCatggtgagcaagggcga"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    5423..5439
                     /label="PCR FWD"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             5423..6130
                     /label="mCherry"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     CDS             5423..6130
                     /label="Translation 5423-6130"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYK"
     misc_feature    6111..6130
                     /label="PCR REV"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(6111..6154)
                     /label="CA-0042.R"
                     /note="sequence: agatgagtttttgTTCgggccCTActtgtacagctcgtccatgc"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             6131..6133
                     /label="Translation 6131-6133"
                     /translation="*"
     primer          complement(6133..6154)
                     /label="CA-0127.R2"
                     /note="sequence: agatgagtttttgTTCgggccC"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    6139..6155
                     /label="INF"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             6143..6172
                     /label="c-myc epitope"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     misc_feature    6172..6174
                     /label="New Stop"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             6185..6202
                     /label="Polyhistidine tag"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     misc_feature    6203..6205
                     /label="STOP (missing now)"
                     /ApEinfo_revcolor="#a187be"
                     /ApEinfo_fwdcolor="#a187be"
     polyA_signal    6224..6438
                     /label="BGH polyadenylation sequence"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(6225..6242)
                     /label="BGHR Genewiz"
                     /note="sequence: TAGAAGGCACAGTCGAGG"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     polyA_signal    6471..6494
                     /label="BGH pA"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    6777..6824
                     /label="FRT"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_recomb     6791..6824
                     /label="FRT"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     gene            6832..7852
                     /label="Hygromycin"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     polyA_signal    7984..8114
                     /label="SV40 pA"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     complement(8150..8170)
                     /label="M13-rev"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_binding    complement(8176..8198)
                     /label="LacO"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     promoter        complement(8202..8232)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     rep_origin      8497..425
                     /label="pUC ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     rep_origin      complement(8538..475)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
ORIGIN
        1 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
       61 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
      121 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
      181 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
      241 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
      301 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
      361 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
      421 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
      481 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
      541 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
      601 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
      661 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
      721 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
      781 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
      841 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
      901 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
      961 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     1021 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     1081 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     1141 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     1201 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     1261 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     1321 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     1381 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     1441 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     1501 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     1561 gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct
     1621 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
     1681 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
     1741 atctgcttag ggttaggcgt tttgcgctgc ttcgtacggg ccagatatac gcgttgacat
     1801 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
     1861 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     1921 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     1981 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     2041 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     2101 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     2161 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
     2221 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
     2281 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
     2341 ggtaggcgtg tacggtggga ggtctatata agcagagctc tccctatcag tgatagagat
     2401 ctccctatca gtgatagaga tcgtcgacga gctcgtttag tgaaccgtca gatcgcctgg
     2461 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg
     2521 actctagcgt ttaaacttaa gcttcgccac cATGGACGGT TGGAGAAGGA TGCCCCGGTG
     2581 GGGCCTTTTG CTGCTCCTGT GGGGAAGCTG CACCTTCGGG GCTagcCTCA CTCTCGGTAA
     2641 TACTACTTCC TCAGTCATCT TAACCAACTA CATGGATACG CAGTATTACG GGGAAATCGG
     2701 CATTGGCACA CCCCCTCAGA CTTTTAAGGT AGTTTTTGAT ACTGGGTCTA GCAATGTTTG
     2761 GGTCCCTAGC AGTAAGTGTT CCCGCCTGTA TACAGCGTGT GTTTACCATA AGTTATTTGA
     2821 CGCCTCAGAC TCCTCTAGCT ATAAACACAA CGGCACCGAG CTCACCATCC ACTACAGCAC
     2881 AGGGACTGTT TCGGGGTTTC TGTCCCAGGA CATTATTACA GTGGGAGGTA TTACAGTGAC
     2941 CCAAATGTTT GGGGAGGTGA CCGAGATGCC GGCTCTGCCG TTCATGCTGG CAGAGTTCGA
     3001 CGGGGTCGTT GGCATGGGCT TCATCGAGCA GGCTGTGGGG CGGGTAACCC CCATCTTCGA
     3061 CAACATCATT AGCCAGGGCG TGCTAAAGGA GGACGTGTTC AGCTTTTATT ACAATAGAGA
     3121 TTCTGAAAAC TCTCAGAGTT TGGGCGGCCA GATAGTGCTT GGAGGCAGTG ATCCCCAGCA
     3181 CTACGAAGGC AATTTTCACT ATATAAATCT GATCAAAACT GGGGTGTGGC AGATCCAGAT
     3241 GAAGGGGGTG TCAGTGGGTT CCTCTACGTT ATTGTGCGAG GACGGATGTC TGGCCGTGGT
     3301 AGACACTGGA GCTAGTTACA TCAGCGGCTC AACCAGCTCA ATTGAAAAGC TGATGGAGGC
     3361 ACTGGGAGCA AAAAAGCGGT TGTTCGATTA CGTCGTGAAA TGTAATGAGG GACCTACCCT
     3421 GCCCGACATC TCTTTCCATC TGGGTGGAAA AGAGTATACT CTGACAAGCG CCGATTACGT
     3481 ATTTCAGGAG TCCTACTCGT CCAAAAAACT CTGCACGTTA GCAATCCACG CCATGGACAT
     3541 CCCTCCACCA ACCGGACCTA CCTGGGCTCT GGGGGCCACA TTTATACGAA AATTCTATAC
     3601 CGAGTTCGAT CGCAGGAACA ACCGTATCGG CTTTGCACTT GCCAGGggcg ccgggacagg
     3661 agggtctggg ggtggcactg gcggatctgg aggagactac aaggacgatg acgacaacgg
     3721 cggatcgggg gggtctggtg gtagtggcgg ttcgggggga GGCACAggtg gatccATGGC
     3781 TCTCATTGTC TTGGGCGGTG TGGCCGGGTT ACTGCTGTTC ATCGGACTTG GCATATTTTT
     3841 CGGGGGCGGC TCTctcgaga acttgtactt ccagagtGGC AGTggaGGTA CCCTTCTCGA
     3901 AGCCCTGACC TTAGCCAGTG CACGGGGACC CCTGAGGAAG AGATCTGTGC CAATGGCTAA
     3961 GGCGAAACCG AAATTCTCCA TCTCACCTGA CAGCTTGAGC GAAGGTCGGG GGAGTTTGCT
     4021 TACCTGTGGC GATGTCGAGG AGAATCCAGG ACCCATGCAC TCCTCCGCGC TCTTGTGTTG
     4081 CCTAGTTTTA TTGACCGGAG TCCGCGCATC ACCTGGGCAA GGCACTCAGA GTGAAAATTC
     4141 TTGCACTCAC TTCCCTGGAA TTCTCCCCAA CATGCTCAGA GACCTGCGAG ATGCTTTCTC
     4201 TAGAGTCAAA ACGTTCTTTC AAATGAAGGA CCAGCTAGAT AATCTTTTGC TGAAAGAGTC
     4261 CCTTCTCGAG GATTTTAAAG GCTATCTGGG CTGTCAGGCT CTTAGCGAGA TGATACAGTT
     4321 CTACCTCGAG GAGGTGATGC CACAGGCTGA GAACCAAGAC CCAGACATTA AAGCCCATGT
     4381 AATCTCACTG GGGGAAAACC TGAACACATT ACGCCTGCGG CTGCGGAGGT GCCATAGGCT
     4441 GCTGCCTTGT GAAAATGGTG GGGGCAGTGG AGGCAAGAGC AAGGCCGTGG AACAGGTGAA
     4501 GAATGCCTTT AATAAGCTGC AGGAAAAGGG GATCTACAAG GCAATGAGCG AGTTTGATAT
     4561 ATTCATCAAC TATATCGAGG CCTACATGAC CATGAAAATT CGTAACGGCG CCTACATCCA
     4621 TCCCTTCCAC CTGCTCTACC ACAACAAGAG CGGCGGTGGG GGATCTGGTG GAGGGGGATC
     4681 AGGAGGTGGC GGATCCCATG GCACTGAGCT TCCATCTCCT CCTTCTGTAT GGTTCGAAGC
     4741 AGAGTTCTTC CATCACATTC TGCATTGGAC TCCCATTCCA AACCAATCGG AGAGCACCTG
     4801 CTACGAAGTG GCTCTGTTAA GATACGGCAT TGAGTCTTGG AACAGCATCA GCAACTGCTC
     4861 ACAAACGCTC TCTTATGATT TGACCGCGGT GACACTTGAC CTCTACCACT CAAACGGCTA
     4921 TAGAGCCCGG GTTCGAGCCG TGGATGGCTC CCGACACTCC AACTGGACCG TCACTAATAC
     4981 ACGGTTTAGC GTGGACGAGG TGACCCTGAC AGTGGGAAGT GTGAACCTCG AAATTCATAA
     5041 CGGGTTTATT CTGGGAAAAA TACAGCTGCC TCGTCCAAAG ATGGCACCAG CCAACGACAC
     5101 GTACGAAAGC ATTTTCAGCC ACTTCCGGGA GTACGAAATC GCAATCCGCA AGGTACCGGG
     5161 TAATTTTACT TTTACCCACA AGAAGGTTAA GCACGAAAAC TTCAGCTTGT TAACCTCTGG
     5221 CGAGGTCGGA GAGTTTTGCG TGCAAGTGAA GCCTAGCGTC GCTTCACGCT CCAATAAAGG
     5281 TATGTGGAGT AAAGAGGAGT GCATCAGTCT CACAAGGCAG TATTTTACGG TCACCAATGA
     5341 GCAGAAGCTC ATCAGCGAAG AGGACCTGGA GGGACGGGGA AGTTTGCTTA CCTGTGGCGA
     5401 TGTCGAGGAG AATCCAGGTC CCatggtgag caagggcgag gaggataaca tggccatcat
     5461 caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga
     5521 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
     5581 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg
     5641 ctccaaggcc tacgtgaagc accccgccga catTcccgac tacttgaagc tgtccttccc
     5701 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac
     5761 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa
     5821 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga
     5881 gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa
     5941 ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca
     6001 gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta
     6061 caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga
     6121 gctgtacaag TAGaggcccG AAcaaaaact catctcagaa gaggatctga atagcgccgt
     6181 cgaccatcat cataatcatc attgagttta aacccgctga tcagcctcga ctgtgccttc
     6241 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     6301 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     6361 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
     6421 tagcaggcat gctggggatg cggtgggctc tatggagatc tatcgatgtt ctggggatgc
     6481 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
     6541 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     6601 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     6661 gttcgccggc tttccccgtc aagctctaaa tcgggggtcc ctttagggtt ccgatttagt
     6721 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tacctagaag
     6781 ttcctattcc gaagttccta ttctctagaa agtataggaa cttccttggc caaaaagcct
     6841 gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac
     6901 ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt aggagggcgt
     6961 ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg ttatgtttat
     7021 cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg ggaattcagc
     7081 gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca agacctgcct
     7141 gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg
     7201 gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat cggtcaatac
     7261 actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca ctggcaaact
     7321 gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg
     7381 gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc caacaatgtc
     7441 ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat gttcggggat
     7501 tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg tatggagcag
     7561 cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccgcg gctccgggcg
     7621 tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat
     7681 gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc
     7741 gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg tgtagaagta
     7801 ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagga atagcacgta
     7861 ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     7921 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     7981 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     8041 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     8101 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     8161 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     8221 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     8281 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     8341 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     8401 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     8461 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     8521 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     8581 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     8641 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     8701 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctt
//