LOCUS       CA-0034_CMVTO-hIL6-rens 6914 bp ds-DNA     circular     26-NOV-2024
DEFINITION  .
COMMENT     Concatenation of 4 sequences.
FEATURES             Location/Qualifiers
     CDS             complement(403..1263)
                     /label="Amp (bla)"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     CDS             complement(406..1065)
                     /label="AmpR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     promoter        complement(1264..1362)
                     /label="bla promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          1475..1495
                     /label="FlpInF1"
                     /note="sequence: agtatctgctccctgcttgtg"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     promoter        1626..2253
                     /label="CMV"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        1705..2008
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     promoter        2009..2212
                     /label="CMV promoter"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          2163..2183
                     /label="CMV-Forward Genewiz"
                     /note="sequence: CGCAAATGGGCGGTAGGCGTG"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     TATA_signal     2198..2204
                     /label="TATA box"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2214..2232
                     /label="TetO"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2214..2253
                     /label="TetR binding sites"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2235..2253
                     /label="TetO"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          2314..2334
                     /label="CMVF1"
                     /note="sequence: tgacctccatagaagacaccg"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          2335..2350
                     /label="ToFlpF"
                     /note="sequence: GGACCGATCCAGCCTC"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    2377..2384
                     /label="Kozak"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2385..2471
                     /label="hIL6 Signal Peptide"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     CDS             2385..3020
                     /label="Translation 2385-3020"
                     /translation="MNSFSTSAFGPVAFSLGLLLVLPAAFPAPVPPGEDSKDVAAPHRQPLTSSERIDKQIRYILDGISALRKETCNKSNMCESSKEALAENNLNLPKMAEKDGCFQSGFNEETCLVKIITGLLEFEVYLEYLQNRFESSEEQARAVQMSTKVLIQFLQKKAKNLDAITTPDPTTNASLLTKLQAQNQWLQDMTTHLILRSFKEFLQSSLRALRQM"
     misc_feature    2472..3020
                     /label="IL-6 (Human)"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          2808..2833
                     /label="IL6.seq"
                     /note="sequence: GCTGTCCAGATGTCTACCAAAGTATT"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             3021..3026
                     /label="Translation 3021-3026"
                     /translation="TG"
     misc_feature    3021..3026
                     /label="for cloning"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          3021..4141
                     /label="Rens_IL6Ra_M4.TWIST"
                     /note="sequence: accggTggcGGCGGTAGCggaTATATTCACCCATTCCATCTGGTTATTCATAACGAGTCGGGCGGTGGTACCGGCCTTGCGCCGAGAAGATGCCCCGCCCAAGAGGTGGCACGGGGAGTTCTGACCAGTCTCCCAGGCGATTCTGTGACTCTGACCTGCCCTGGTGTAGAACCGGAAGACAATGCAACCGTCCACTGGGTGTTGAGAAAGCCAGCAGCGGGAAGCCATCCCTCCAGGTGGGCCGGCATGGGACGACGCCTGCTGCTGAGGAGTGTGCAGCTACACGATTCTGGTAACTACTCTTGTTATAGGGCCGGCCGGCCGGCTGGCACCGTCCATTTACTCGTGGATGTGCCTCCCGAGGAGCCCCAATTGAGTTGCTTTCGAAAAAGCCCACTGTCCAATGTAGTCTGCGAGTGGGGTCCACGGTCGACCCCCTCCCTCACAACGAAAGCGGTCTTACTTGTTAGGAAGTTCCAGAACAGCCCTGCTGAGGACTTCCAGGAACCTTGTCAGTACAGTCAAGAGTCTCAGAAGTTTAGCTGTCAATTGGCAGTCCCAGAGGGGGACAGCAGCTTTTATATCGTATCAATGTGCGTGGCCTCCTCCGTGGGGAGTAAGTTCTCCAAGACCCAGACTTTCCAGGGCTGTGGCATTCTTCAGCCTGACCCACCAGCCAACATTACTGTGACCGCCGTAGACAGAGATCCCCGTTGGCTGAGCGTTACTTGGCAAGACCCCCATAGTTGGAACTCATCTTTCTATCGGCTCCGCTTTGAGCTCCGTTACCGCGCCGAACGCAGCAAGACATTTACGACATGGATGGTCAAAGATCTTCAGCACCACTGTGTGATATCTGTGGCTTGGTCCGGGCTGAGGCACGTTGTCCAGCTGAGAGCCCAGGAGGAATTTGGACAGGGGGAGTGGTCCGAGTGGTCACCAGAAGCTATGGGGACTCCTTGGACAGAATCTCGATCCCCCCCCGCTGAGAATGAAGTGTCAACCCCCATGCAGGCACTGACAACAAATAAAGATGACGATAATATCCTATTCCGGGACTCAGCCAACGCTACTTCGTTGCCTGTTCAGGACAGCTCTTCAGTGCCTCTGCCTTAGggccc"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             3027..3095
                     /label="Translation 3027-3095"
                     /translation="GGGSGYIHPFHLVIHNESGGGTG"
     misc_feature    3042..3080
                     /label="Renin cleavage site"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    3096..4133
                     /label="shIL-6Ra (Mut-4)"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             3096..4133
                     /label="Translation 3096-4133"
                     /translation="LAPRRCPAQEVARGVLTSLPGDSVTLTCPGVEPEDNATVHWVLRKPAAGSHPSRWAGMGRRLLLRSVQLHDSGNYSCYRAGRPAGTVHLLVDVPPEEPQLSCFRKSPLSNVVCEWGPRSTPSLTTKAVLLVRKFQNSPAEDFQEPCQYSQESQKFSCQLAVPEGDSSFYIVSMCVASSVGSKFSKTQTFQGCGILQPDPPANITVTAVDRDPRWLSVTWQDPHSWNSSFYRLRFELRYRAERSKTFTTWMVKDLQHHCVISVAWSGLRHVVQLRAQEEFGQGEWSEWSPEAMGTPWTESRSPPAENEVSTPMQALTTNKDDDNILFRDSANATSLPVQDSSSVPLP"
     primer          3461..3482
                     /label="IL6RaM4.seq"
                     /note="sequence: CCTCACAACGAAAGCGGTCTTA"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             4134..4136
                     /label="Translation 4134-4136"
                     /translation="*"
     CDS             4145..4174
                     /label="c-myc epitope"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     misc_feature    4174..4176
                     /label="New Stop"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             4187..4204
                     /label="Polyhistidine tag"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     misc_feature    4205..4207
                     /label="STOP (missing now)"
                     /ApEinfo_revcolor="#a187be"
                     /ApEinfo_fwdcolor="#a187be"
     primer          complement(4208..4226)
                     /label="ToFlpR"
                     /note="sequence: CTGATCAGCGGGTTTAAAC"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     polyA_signal    4226..4440
                     /label="BGH polyadenylation sequence"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(4227..4244)
                     /label="BGHR Genewiz"
                     /note="sequence: TAGAAGGCACAGTCGAGG"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(4306..4325)
                     /label="BghR1"
                     /note="sequence: attaggaaaggacagtggga"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          4417..4435
                     /label="bghF"
                     /note="sequence: AGACAATAGCAGGCATGCT"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     polyA_signal    4473..4496
                     /label="BGH pA"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(4575..4590)
                     /label="FlpInR"
                     /note="sequence: cgcgtaaccaccacac"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          4707..4725
                     /label="FrtF"
                     /note="sequence: agggttccgatttagtgct"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    4779..4826
                     /label="FRT"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_recomb     4793..4826
                     /label="FRT"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     gene            4834..5854
                     /label="Hygromycin"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             4834..5854
                     /label="Translation 4834-5854"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     primer          5782..5800
                     /label="Hyg_F"
                     /note="sequence: ACCGATGGCTGTGTAGAAG"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     polyA_signal    5986..6116
                     /label="SV40 pA"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(5999..6024)
                     /label="SV40PA_R"
                     /note="sequence: GCTTTATTTGTAACCATTATAAGCTG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(6023..6042)
                     /label="SV40pA-R Genewiz"
                     /note="sequence: GAAATTTGTGATGCTATTGC"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer_bind     complement(6152..6172)
                     /label="M13-rev"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(6170..6189)
                     /label="M13-48REV Genewiz"
                     /note="sequence: CGGATAACAATTTCACACAG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_binding    complement(6178..6200)
                     /label="LacO"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     promoter        complement(6204..6234)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          6448..6467
                     /label="pUCori5F"
                     /note="sequence: AAGGCGGTAATACGGTTATC"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     rep_origin      6499..258
                     /label="pUC ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     rep_origin      complement(6540..308)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          complement(6619..6638)
                     /label="pUCoriR"
                     /note="sequence: tatagtcctgtcgggtttcg"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
ORIGIN
        1 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
       61 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
      121 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
      181 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
      241 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
      301 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
      361 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
      421 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
      481 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
      541 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
      601 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
      661 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
      721 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
      781 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
      841 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
      901 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
      961 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     1021 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     1081 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     1141 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     1201 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     1261 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     1321 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     1381 aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc
     1441 agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg
     1501 gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac
     1561 aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgtac gggccagata
     1621 tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
     1681 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
     1741 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
     1801 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
     1861 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
     1921 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
     1981 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
     2041 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
     2101 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
     2161 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctctccctat
     2221 cagtgataga gatctcccta tcagtgatag agatcgtcga cgagctcgtt tagtgaaccg
     2281 tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg
     2341 atccagcctc cggactctag cgtttaaact taagcttcgc caccATGAAC AGCTTCTCTA
     2401 CCAGCGCTTT TGGACCCGTG GCCTTTTCGT TGGGCCTGCT TCTCGTTCTT CCTGCCGCAT
     2461 TCCCTGCACC AGTCCCCCCA GGCGAAGATT CTAAAGATGT GGCTGCTCCA CACAGACAGC
     2521 CCCTTACTAG CAGTGAACGT ATTGATAAAC AGATCAGGTA CATCCTGGAC GGAATCTCAG
     2581 CCCTAAGAAA AGAGACATGC AATAAGTCCA ACATGTGTGA GAGTTCCAAG GAGGCCCTCG
     2641 CCGAGAATAA CCTGAATCTA CCCAAGATGG CCGAGAAGGA CGGTTGTTTC CAGAGTGGGT
     2701 TTAACGAAGA GACTTGCCTG GTGAAAATTA TAACCGGGCT GCTGGAATTC GAAGTGTACC
     2761 TGGAGTATTT GCAAAATCGG TTCGAGTCAT CTGAAGAGCA GGCCAGGGCT GTCCAGATGT
     2821 CTACCAAAGT ATTAATCCAG TTCTTGCAGA AGAAAGCTAA GAACCTGGAT GCAATAACAA
     2881 CTCCTGACCC CACCACAAAC GCGAGCCTGC TTACAAAGCT CCAGGCGCAG AATCAGTGGT
     2941 TACAAGACAT GACGACCCAT CTGATTCTCC GATCATTTAA GGAATTTTTG CAATCCTCCC
     3001 TCCGGGCACT GCGCCAAATG accggTggcG GCGGTAGCgg aTATATTCAC CCATTCCATC
     3061 TGGTTATTCA TAACGAGTCG GGCGGTGGTA CCGGCCTTGC GCCGAGAAGA TGCCCCGCCC
     3121 AAGAGGTGGC ACGGGGAGTT CTGACCAGTC TCCCAGGCGA TTCTGTGACT CTGACCTGCC
     3181 CTGGTGTAGA ACCGGAAGAC AATGCAACCG TCCACTGGGT GTTGAGAAAG CCAGCAGCGG
     3241 GAAGCCATCC CTCCAGGTGG GCCGGCATGG GACGACGCCT GCTGCTGAGG AGTGTGCAGC
     3301 TACACGATTC TGGTAACTAC TCTTGTTATA GGGCCGGCCG GCCGGCTGGC ACCGTCCATT
     3361 TACTCGTGGA TGTGCCTCCC GAGGAGCCCC AATTGAGTTG CTTTCGAAAA AGCCCACTGT
     3421 CCAATGTAGT CTGCGAGTGG GGTCCACGGT CGACCCCCTC CCTCACAACG AAAGCGGTCT
     3481 TACTTGTTAG GAAGTTCCAG AACAGCCCTG CTGAGGACTT CCAGGAACCT TGTCAGTACA
     3541 GTCAAGAGTC TCAGAAGTTT AGCTGTCAAT TGGCAGTCCC AGAGGGGGAC AGCAGCTTTT
     3601 ATATCGTATC AATGTGCGTG GCCTCCTCCG TGGGGAGTAA GTTCTCCAAG ACCCAGACTT
     3661 TCCAGGGCTG TGGCATTCTT CAGCCTGACC CACCAGCCAA CATTACTGTG ACCGCCGTAG
     3721 ACAGAGATCC CCGTTGGCTG AGCGTTACTT GGCAAGACCC CCATAGTTGG AACTCATCTT
     3781 TCTATCGGCT CCGCTTTGAG CTCCGTTACC GCGCCGAACG CAGCAAGACA TTTACGACAT
     3841 GGATGGTCAA AGATCTTCAG CACCACTGTG TGATATCTGT GGCTTGGTCC GGGCTGAGGC
     3901 ACGTTGTCCA GCTGAGAGCC CAGGAGGAAT TTGGACAGGG GGAGTGGTCC GAGTGGTCAC
     3961 CAGAAGCTAT GGGGACTCCT TGGACAGAAT CTCGATCCCC CCCCGCTGAG AATGAAGTGT
     4021 CAACCCCCAT GCAGGCACTG ACAACAAATA AAGATGACGA TAATATCCTA TTCCGGGACT
     4081 CAGCCAACGC TACTTCGTTG CCTGTTCAGG ACAGCTCTTC AGTGCCTCTG CCTTAGggcc
     4141 cGAAcaaaaa ctcatctcag aagaggatct gaatagcgcc gtcgaccatc atcataatca
     4201 tcattgagtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     4261 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     4321 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     4381 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     4441 tgcggtgggc tctatggaga tctatcgatg ttctggggat gcggtgggct ctatggcttc
     4501 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
     4561 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     4621 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     4681 tcaagctcta aatcgggggt ccctttaggg ttccgattta gtgctttacg gcacctcgac
     4741 cccaaaaaac ttgattaggg tgatggttca cgtacctaga agttcctatt ccgaagttcc
     4801 tattctctag aaagtatagg aacttccttg gccaaaaagc ctgaactcac cgcgacgtct
     4861 gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag
     4921 ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta
     4981 aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc
     5041 gcgctcccga ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc
     5101 atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct
     5161 gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg
     5221 agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc
     5281 atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc
     5341 agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa
     5401 gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc
     5461 ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc
     5521 aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag
     5581 cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt
     5641 cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag
     5701 ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc
     5761 cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac
     5821 cgacgcccca gcactcgtcc gagggcaaag gaatagcacg tactacgaga tttcgattcc
     5881 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     5941 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
     6001 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     6061 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
     6121 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     6181 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     6241 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     6301 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     6361 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     6421 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     6481 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     6541 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     6601 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     6661 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     6721 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     6781 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     6841 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     6901 ctggcagcag ccac
//