LOCUS       AVEC3__C-mEGFP_02              7125 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             1885..2958
                     /note="pLannotate"
                     /label="pVS1 RepA"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             6170..6979
                     /note="pLannotate"
                     /label="KanR"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             827..1456
                     /note="pLannotate"
                     /label="pVS1 StaA"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      1..589
                     /note="pLannotate"
                     /label="ori"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        4465..4806
                     /note="pLannotate"
                     /label="CaMV 35S promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="99.1304347826087"
                     /fragment="False"
                     /other="promoter"
     rep_origin      3024..3218
                     /note="pLannotate"
                     /label="pVS1 oriV"
                     /database="addgene"
                     /identity="99.49"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     polyA_signal    complement(5795..5954)
                     /note="pLannotate"
                     /label="CaMV poly(A) signal"
                     /database="addgene"
                     /identity="98.137"
                     /match_length="91.47727272727273"
                     /fragment="True"
                     /other="polyA signal"
     misc_feature    3385..3408
                     /note="pLannotate"
                     /label="LB T-DNA repeat"
                     /database="addgene"
                     /identity="95.833"
                     /match_length="96.0"
                     /fragment="False"
                     /other="misc feature"
     misc_feature    3430..3453
                     /note="pLannotate"
                     /label="LB T-DNA repeat"
                     /database="addgene"
                     /identity="95.833"
                     /match_length="96.0"
                     /fragment="False"
                     /other="misc feature"
     CDS             4998..5710
                     /note="pLannotate"
                     /label="mEGFP"
                     /database="fpbase"
                     /identity="100.0"
                     /match_length="99.581589958159"
                     /fragment="False"
                     /other="CDS"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgactga agaaccgagc
      721 gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg
      781 cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc
      841 tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc
      901 cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg
      961 cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac
     1021 gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc
     1081 ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt
     1141 gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg
     1201 cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg
     1261 cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga
     1321 gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct
     1381 tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa
     1441 atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta
     1501 agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac
     1561 acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag
     1621 atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag
     1681 ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg
     1741 cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg
     1801 aacgggcggt tggccaggcg taagcggctg ggttgcctgc cggccctgca atggcactgg
     1861 aacccccaag cccgaggaat cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc
     1921 ggcgcggcgc tgggtgatga cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg
     1981 caacgcatcg aggcagaagc acgccccggt gaatcgtggc aagcggccgc tgatcgaatc
     2041 cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag
     2101 ggcgacgagc aaccagattt tttcgttccg atgctctatg acgtgggcac ccgcgatagt
     2161 cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag
     2221 gtgatccgct acgagcttcc agacgggcac gtagaggttt ccgcagggcc ggccggcatg
     2281 gccagtgtgt gggattacga cctggtactg atggcggttt cccatctaac cgaatccatg
     2341 aaccgatacc gggaagggaa gggagacaag cccggccgcg tgttccgtcc acacgttgcg
     2401 gacgtactca agttctgccg gcgagccgat ggcggaaagc agaaagacga cctggtagaa
     2461 acctgcattc ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac
     2521 ggccgcctgg tgacggtatc cgagggtgaa gccttgatta gccgctacaa gatcgtaaag
     2581 agcgaaaccg ggcggccgga gtacatcgag atcgagctag ctgattggat gtaccgcgag
     2641 atcacagaag gcaagaaccc ggacgtgctg acggttcacc ccgattactt tttgatcgat
     2701 cccggcatcg gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc
     2761 agatggttgt tcaagacgat ctacgaacgc agtggcagcg ccggagagtt caagaagttc
     2821 tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc cggagtacga tttgaaggag
     2881 gaggcggggc aggctggccc gatcctagtc atgcgctacc gcaacctgat cgagggcgaa
     2941 gcatccgccg gttcctaatg tacggagcag atgctagggc aaattgccct agcaggggaa
     3001 aaaggtcgaa aaggtctctt tcctgtggat agcacgtaca ttgggaaccc aaagccgtac
     3061 attgggaacc ggaacccgta cattgggaac ccaaagccgt acattgggaa ccggtcacac
     3121 atgtaagtga ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa
     3181 cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc
     3241 gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg
     3301 cgtcggccta tcgcggccgc tggccgctca aaaatggctg gcctacggcc aggcaatcta
     3361 ccagcgcgcg gacaagctag gattggcagg atatattcag ttgtaaatgc gagctgttgg
     3421 ctggctggtg gcaggatata ttcagttgta aataaattga cgcttagaca acttaataac
     3481 acattgcgga cgtttttaat gtactgaatt aacgccgaat taattcgagc tctctagaat
     3541 cgtcgactcg agactagagc caagctgatc tcctttgccc cggagatcac catggacgac
     3601 tttctctatc tctacgatct aggaagaaag ttcgacggag aaggtgacga taccatgttc
     3661 accaccgata atgagaagat tagcctcttc aatttcagaa agaatgctga cccacagatg
     3721 gttagagagg cttacgcggc aggtctcatc aagacgatct acccgagtaa taatctccag
     3781 gagatcaaat accttcccaa gaaggttaaa gatgcagtca aaagattcag gactaactgc
     3841 atcaagaaca cagagaaaga tatatttctc aagatcagaa gtactattcc agtatggacg
     3901 attcaaggct tgcttcataa accaaggcaa gtaatagaga ttggagtctc taagaaagta
     3961 gttcctactg aatcaaaggc catggagtca aaaattcaga tcgaggatct aacagaactc
     4021 gccgtgaaga ctggcgaaca gttcatacag agtcttttac gactcaatga caagaagaaa
     4081 atcttcgtca acatggtgga gcacgacact ctcgtctact ccaagaatat caaagataca
     4141 gtctcagaag accaaagggc tattgagact tttcaacaaa gggtaatatc gggaaacctc
     4201 ctcggattcc attgcccagc tatctgtcac ttcatcaaaa ggacagtaga aaaggaaggt
     4261 ggcacctaca aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga tgcctctgcc
     4321 gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt
     4381 ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac
     4441 gcacaatccc actatccttc gcaatgagac ttttcaacaa agggtaatat cgggaaacct
     4501 cctcggattc cattgcccag ctatctgtca cttcatcaaa aggacagtag aaaaggaagg
     4561 tggcacctac aaatgccatc attgcgataa aggaaaggct atcgttcaag atgcctctgc
     4621 cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt
     4681 tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg taagggatga
     4741 cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt
     4801 ggagagaggc ctatttttac aacaattacc aacaacaaca aacaacaaac aacattacaa
     4861 ttactattta caattacagg cctcccgggt caggaagcga aacaccagga acatcagaaa
     4921 gtgcaacacc agaatctgga tccggctctg aaactcctgg tactagtgaa tcggctaccc
     4981 ctgaatcagg gtcctctgtt tcaaagggag aggagttgtt cactggagtt gttcctatct
     5041 tggttgagct tgatggagat gttaacggac acaagttctc agtttctgga gagggagagg
     5101 gagatgctac ttacggaaag ctcactttga agttcatctg cactactggt aagcttcctg
     5161 ttccatggcc aacccttgtt accaccctta cctacggtgt tcaatgcttc tctcgttacc
     5221 ctgatcacat gaagcaacac gatttcttca agtctgctat gcctgagggt tacgtccaag
     5281 agagaaccat cttctttaag gatgatggta actacaagac tagagctgag gtcaagtttg
     5341 agggtgacac tcttgtcaac agaatcgaac ttaagggtat cgactttaag gaagacggta
     5401 acatccttgg tcacaagttg gaatacaact acaactctca caacgtttac atcatggctg
     5461 acaagcagaa gaacggtatc aaggttaact ttaagatcag acacaacatt gaagatggtt
     5521 ctgttcagtt ggctgatcac taccagcaga acactcctat tggtgatggt cctgttttgt
     5581 tgccagataa ccactacttg tctactcagt ctaaattgag taaagatcca aacgagaaga
     5641 gagatcacat ggttcttctt gagtttgtta ctgctgctgg tattactctt ggaatggatg
     5701 agctttacaa gtgagggtcc tctgtcctga tcacggccat gctagagtcc gcaaaaatca
     5761 ccagtctctc tctacaaatc tatctctctc tatttttctc cagaataatg tgtgagtagt
     5821 tcccagataa gggaattagg gttcttatag ggtttcgctc atgtgttgag catataagaa
     5881 acccttagta tgtatttgta tttgtaaaat acttctatca ataaaatttc taattcctaa
     5941 aaccaaaatc cagtgacctg gtaacctggc aggatatatg cggttgtaat tttggcagga
     6001 tatatgcggt tgtaattcat ttttattgtc taaatttctg tatttgtttg tttgcaatta
     6061 gatccacgtt gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc
     6121 atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat
     6181 tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta
     6241 taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa
     6301 gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac
     6361 agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca
     6421 ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc
     6481 attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt
     6541 gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt
     6601 atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt
     6661 tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt
     6721 gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt
     6781 tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata
     6841 ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg
     6901 gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat
     6961 gctcgatgag tttttctaat cagaattggt taattggttg taacactggc agagcattac
     7021 gctgacttga cgggacggcg caagctcatg accaaaatcc cttaacgtga gttacgcgtc
     7081 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttc
//
