LOCUS       AVEC4__N-mEGFP_34              8321 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             1885..2958
                     /note="pLannotate"
                     /label="pVS1 RepA"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             7366..8175
                     /note="pLannotate"
                     /label="KanR"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             827..1456
                     /note="pLannotate"
                     /label="pVS1 StaA"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      1..589
                     /note="pLannotate"
                     /label="ori"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             complement(3807..4358)
                     /note="pLannotate"
                     /label="BlpR"
                     /database="addgene"
                     /identity="99.819"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        complement(4364..4744)
                     /note="pLannotate"
                     /label="MAS promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        5671..6012
                     /note="pLannotate"
                     /label="CaMV 35S promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="99.1304347826087"
                     /fragment="False"
                     /other="promoter"
     terminator      3545..3797
                     /note="pLannotate"
                     /label="MAS terminator"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     rep_origin      3024..3218
                     /note="pLannotate"
                     /label="pVS1 oriV"
                     /database="addgene"
                     /identity="99.49"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     polyA_signal    complement(6991..7150)
                     /note="pLannotate"
                     /label="CaMV poly(A) signal"
                     /database="addgene"
                     /identity="98.137"
                     /match_length="91.47727272727273"
                     /fragment="True"
                     /other="polyA signal"
     misc_feature    3385..3408
                     /note="pLannotate"
                     /label="LB T-DNA repeat"
                     /database="addgene"
                     /identity="95.833"
                     /match_length="96.0"
                     /fragment="False"
                     /other="misc feature"
     misc_feature    3430..3453
                     /note="pLannotate"
                     /label="LB T-DNA repeat"
                     /database="addgene"
                     /identity="95.833"
                     /match_length="96.0"
                     /fragment="False"
                     /other="misc feature"
     CDS             6099..6814
                     /note="pLannotate"
                     /label="mEGFP"
                     /database="fpbase"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgactga agaaccgagc
      721 gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg
      781 cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc
      841 tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc
      901 cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg
      961 cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac
     1021 gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc
     1081 ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt
     1141 gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg
     1201 cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg
     1261 cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga
     1321 gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct
     1381 tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa
     1441 atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta
     1501 agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac
     1561 acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag
     1621 atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag
     1681 ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg
     1741 cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg
     1801 aacgggcggt tggccaggcg taagcggctg ggttgcctgc cggccctgca atggcactgg
     1861 aacccccaag cccgaggaat cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc
     1921 ggcgcggcgc tgggtgatga cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg
     1981 caacgcatcg aggcagaagc acgccccggt gaatcgtggc aagcggccgc tgatcgaatc
     2041 cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag
     2101 ggcgacgagc aaccagattt tttcgttccg atgctctatg acgtgggcac ccgcgatagt
     2161 cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag
     2221 gtgatccgct acgagcttcc agacgggcac gtagaggttt ccgcagggcc ggccggcatg
     2281 gccagtgtgt gggattacga cctggtactg atggcggttt cccatctaac cgaatccatg
     2341 aaccgatacc gggaagggaa gggagacaag cccggccgcg tgttccgtcc acacgttgcg
     2401 gacgtactca agttctgccg gcgagccgat ggcggaaagc agaaagacga cctggtagaa
     2461 acctgcattc ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac
     2521 ggccgcctgg tgacggtatc cgagggtgaa gccttgatta gccgctacaa gatcgtaaag
     2581 agcgaaaccg ggcggccgga gtacatcgag atcgagctag ctgattggat gtaccgcgag
     2641 atcacagaag gcaagaaccc ggacgtgctg acggttcacc ccgattactt tttgatcgat
     2701 cccggcatcg gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc
     2761 agatggttgt tcaagacgat ctacgaacgc agtggcagcg ccggagagtt caagaagttc
     2821 tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc cggagtacga tttgaaggag
     2881 gaggcggggc aggctggccc gatcctagtc atgcgctacc gcaacctgat cgagggcgaa
     2941 gcatccgccg gttcctaatg tacggagcag atgctagggc aaattgccct agcaggggaa
     3001 aaaggtcgaa aaggtctctt tcctgtggat agcacgtaca ttgggaaccc aaagccgtac
     3061 attgggaacc ggaacccgta cattgggaac ccaaagccgt acattgggaa ccggtcacac
     3121 atgtaagtga ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa
     3181 cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc
     3241 gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg
     3301 cgtcggccta tcgcggccgc tggccgctca aaaatggctg gcctacggcc aggcaatcta
     3361 ccagcgcgcg gacaagctag gattggcagg atatattcag ttgtaaatgc gagctgttgg
     3421 ctggctggtg gcaggatata ttcagttgta aataaattga cgcttagaca acttaataac
     3481 acattgcgga cgtttttaat gtactgaatt aacgccgaat taattcgagc tcttctagag
     3541 atctgataat ttatttgaaa attcataaga aaagcaaacg ttacatgaat tgatgaaaca
     3601 atacaaagac agataaagcc acgcacattt aggatattgg ccgagattac tgaatattga
     3661 gtaagatcac ggaatttctg acaggagcat gtcttcaatt cagcccaaat ggcagttgaa
     3721 atactcaaac cgccccatat gcaggagcgg atcattcatt gtttgtttgg ttgcctttgc
     3781 caacatggga gtccaagatt ctgcagtcaa atctcggtga cgggcaggac cggacggggc
     3841 ggtaccggca ggctgaagtc cagctgccag aaacccacgt catgccagtt cccgtgcttg
     3901 aagccggccg cccgcagcat gccgcggggg gcatatccga gcgcctcgtg catgcgcacg
     3961 ctcgggtcgt tgggcagccc gatgacagcg accacgctct tgaagccctg tgcctccagg
     4021 gacttcagca ggtgggtgta gagcgtggag cccagtcccg tccgctggtg gcggggggag
     4081 acgtacacgg tcgactcggc cgtccagtcg taggcgttgc gtgccttcca ggggccagcg
     4141 taggcgatgc cggcgacctc gccgtccacc tcggcgacga gccagggata gcgctcccgc
     4201 agacggacga ggtcgtccgt ccactcctgc ggttcctgcg gctcggtacg gaagttgacc
     4261 gtgcttgtct cgatgtagtg gttgacgatg gtgcagaccg ccggcatgtc cgcctcggtg
     4321 gcacggcgga tgtcggccgg gcgtcgttct gggctcatcg attcgatttg gtgtatcgag
     4381 attggttatg aaattcagat gctagtgtaa tgtattggta atttgggaag atataatagg
     4441 aagcaaggct atttatccat ttctgaaaag gcgaaatggc gtcaccgcga gcgtcacgcg
     4501 cattccgttc ttgctgtaaa gcgttgtttg gtacactttt gactagcgag gcttggcgtg
     4561 tcagcgtatc tattcaaaag tcgttaatgg ctgcggatca agaaaaagtt ggaatagaaa
     4621 cagaataccc gcgaaattca ggcccggttg ccatgtccta cacgccgaaa taaacgacca
     4681 aattagtaga aaaataaaaa ctgactcgga tacttacgtc acgtcttgcg cactgatttg
     4741 aaaaatctca gctcgaggac tagagccaag ctgatctcct ttgccccgga gatcaccatg
     4801 gacgactttc tctatctcta cgatctagga agaaagttcg acggagaagg tgacgatacc
     4861 atgttcacca ccgataatga gaagattagc ctcttcaatt tcagaaagaa tgctgaccca
     4921 cagatggtta gagaggctta cgcggcaggt ctcatcaaga cgatctaccc gagtaataat
     4981 ctccaggaga tcaaatacct tcccaagaag gttaaagatg cagtcaaaag attcaggact
     5041 aactgcatca agaacacaga gaaagatata tttctcaaga tcagaagtac tattccagta
     5101 tggacgattc aaggcttgct tcataaacca aggcaagtaa tagagattgg agtctctaag
     5161 aaagtagttc ctactgaatc aaaggccatg gagtcaaaaa ttcagatcga ggatctaaca
     5221 gaactcgccg tgaagactgg cgaacagttc atacagagtc ttttacgact caatgacaag
     5281 aagaaaatct tcgtcaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa
     5341 gatacagtct cagaagacca aagggctatt gagacttttc aacaaagggt aatatcggga
     5401 aacctcctcg gattccattg cccagctatc tgtcacttca tcaaaaggac agtagaaaag
     5461 gaaggtggca cctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc
     5521 tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa
     5581 gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg
     5641 gatgacgcac aatcccacta tccttcgcaa tgagactttt caacaaaggg taatatcggg
     5701 aaacctcctc ggattccatt gcccagctat ctgtcacttc atcaaaagga cagtagaaaa
     5761 ggaaggtggc acctacaaat gccatcattg cgataaagga aaggctatcg ttcaagatgc
     5821 ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga
     5881 agacgttcca accacgtctt caaagcaagt ggattgatgt gatatctcca ctgacgtaag
     5941 ggatgacgca caatcccact atccttcgca agacccttcc tctatataag gaagttcatt
     6001 tcatttggag agaggcctat ttttacaaca attaccaaca acaacaaaca acaaacaaca
     6061 ttacaattac tatttacaat tacaggcctc ccgggaccat ggtttcaaag ggagaggagt
     6121 tgttcactgg agttgttcct atcttggttg agcttgatgg agatgttaac ggacacaagt
     6181 tctcagtttc tggagaggga gagggagatg ctacttacgg aaagctcact ttgaagttca
     6241 tctgcactac tggtaagctt cctgttccat ggccaaccct tgttaccacc cttacctacg
     6301 gtgttcaatg cttctctcgt taccctgatc acatgaagca acacgatttc ttcaagtctg
     6361 ctatgcctga gggttacgtc caagagagaa ccatcttctt taaggatgat ggtaactaca
     6421 agactagagc tgaggtcaag tttgagggtg acactcttgt caacagaatc gaacttaagg
     6481 gtatcgactt taaggaagac ggtaacatcc ttggtcacaa gttggaatac aactacaact
     6541 ctcacaacgt ttacatcatg gctgacaagc agaagaacgg tatcaaggtt aactttaaga
     6601 tcagacacaa cattgaagat ggttctgttc agttggctga tcactaccag cagaacactc
     6661 ctattggtga tggtcctgtt ttgttgccag ataaccacta cttgtctact cagtctaaat
     6721 tgagtaaaga tccaaacgag aagagagatc acatggttct tcttgagttt gttactgctg
     6781 ctggtattac tcttggaatg gatgagcttt acaaggggtc aggaagcgaa acaccaggaa
     6841 catcagaaag tgcaacacca gaatctggat ccggctctga aactcctggt actagtgaat
     6901 cggctacccc tgaatcaggg tcctgatcac ggccatgcta gagtccgcaa aaatcaccag
     6961 tctctctcta caaatctatc tctctctatt tttctccaga ataatgtgtg agtagttccc
     7021 agataaggga attagggttc ttatagggtt tcgctcatgt gttgagcata taagaaaccc
     7081 ttagtatgta tttgtatttg taaaatactt ctatcaataa aatttctaat tcctaaaacc
     7141 aaaatccagt gacctggtaa cctggcagga tatatgcggt tgtaattttg gcaggatata
     7201 tgcggttgta attcattttt attgtctaaa tttctgtatt tgtttgtttg caattagatc
     7261 cacgttgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga
     7321 acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa
     7381 cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa
     7441 tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta tgggaagccc
     7501 gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat
     7561 gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt
     7621 atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa aacagcattc
     7681 caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc
     7741 ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt
     7801 cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat
     7861 gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca
     7921 ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac
     7981 gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag
     8041 gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt
     8101 tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc
     8161 gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag cattacgctg
     8221 acttgacggg acggcgcaag ctcatgacca aaatccctta acgtgagtta cgcgtcgttc
     8281 cactgagcgt cagaccccgt agaaaagatc aaaggatctt c
//
